BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060680.seq (630 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 65 7e-12 SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi... 30 0.32 SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces po... 26 5.2 SPBC36B7.05c |||phosphatidylinositol|Schizosaccharomyces pombe|c... 25 6.8 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.0 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 65.3 bits (152), Expect = 7e-12 Identities = 22/38 (57%), Positives = 29/38 (76%) Frame = +2 Query: 389 DICRICHCESEPQNPLLAPCYCSGSLKYVHQSCLQQWL 502 +ICR+C CE P +PL PC C+GS++YVHQ CL +WL Sbjct: 6 EICRVCRCEGAPDSPLFHPCKCTGSIRYVHQECLVEWL 43 >SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1053 Score = 29.9 bits (64), Expect = 0.32 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +2 Query: 185 HFSFQVNRKCILTYCNFCVNTFFIMPVQQI 274 HFSF + I +YCNF + FF+ I Sbjct: 320 HFSFGIVVLAIFSYCNFGIKQFFLFAAVMI 349 >SPCC1235.03 |||SMR and CUE domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 399 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/39 (30%), Positives = 21/39 (53%) Frame = -2 Query: 566 LILVCMMKLNLHSSQDXVSVPSTTAASNSGVRI*GFLNN 450 L ++C++K H+ + + S TA SNS + LN+ Sbjct: 185 LWILCLIKETQHNEMGNIKLSSQTAKSNSSTQTKTCLND 223 >SPBC36B7.05c |||phosphatidylinositol|Schizosaccharomyces pombe|chr 2|||Manual Length = 279 Score = 25.4 bits (53), Expect = 6.8 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = +2 Query: 206 RKCILTYCNFCVNTFFIMPVQQISVKNSSEI 298 R C +C C N+F +PV +SV + ++ Sbjct: 50 RWCGKLFCYNCCNSFAKLPVSSVSVDPTEDL 80 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 25.0 bits (52), Expect = 9.0 Identities = 7/12 (58%), Positives = 10/12 (83%) Frame = +2 Query: 470 YVHQSCLQQWLT 505 + HQ+C+ QWLT Sbjct: 788 FFHQACIDQWLT 799 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,375,801 Number of Sequences: 5004 Number of extensions: 46028 Number of successful extensions: 122 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 120 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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