BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060676.seq (630 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g10040.1 68416.m01204 expressed protein est match 31 0.83 At2g27930.1 68415.m03385 zinc-binding family protein similar to ... 30 1.5 At5g57230.1 68418.m07150 expressed protein 29 1.9 At5g61670.2 68418.m07738 expressed protein 29 2.5 At5g61670.1 68418.m07737 expressed protein 29 2.5 At5g58840.1 68418.m07373 subtilase family protein contains simil... 29 2.5 At4g17460.1 68417.m02612 homeobox-leucine zipper protein 1 (HAT1... 29 2.5 At1g75210.1 68414.m08737 5' nucleotidase family protein contains... 28 4.4 At5g10660.1 68418.m01234 calmodulin-binding protein-related cont... 28 5.9 At4g24750.1 68417.m03542 expressed protein 28 5.9 >At3g10040.1 68416.m01204 expressed protein est match Length = 431 Score = 30.7 bits (66), Expect = 0.83 Identities = 16/65 (24%), Positives = 31/65 (47%) Frame = -1 Query: 588 EVEGLVIEHSLKEWRRTRP*LEQSMVSVKMTPERERVVATRVAETWTEATLESTKIWAGA 409 E EG+ +E +W R R E+ M K+ +R R+ R+ + +E ++ + Sbjct: 362 EWEGVEMEKQRVKWMRYRSKKEREMEKAKLDNQRRRLETERMILMLRRSEIELNELQSSG 421 Query: 408 WKVEP 394 +V+P Sbjct: 422 TRVDP 426 >At2g27930.1 68415.m03385 zinc-binding family protein similar to zinc-binding protein [Pisum sativum] GI:16117799; contains Pfam profile PF04640 : Protein of unknown function, DUF597 Length = 135 Score = 29.9 bits (64), Expect = 1.5 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = +1 Query: 388 LSRFNFPGSCPNLRRLQCGFCPRFCYSRRHNSFPFW--SHFYGNHRLFQSR 534 L R NF CP R C FC S ++ +F F+ S F+ +H + Q R Sbjct: 10 LLRTNFFSICPRHRETPRNECNMFCLSCQNAAFCFYCRSSFHIDHPVLQIR 60 >At5g57230.1 68418.m07150 expressed protein Length = 160 Score = 29.5 bits (63), Expect = 1.9 Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 2/32 (6%) Frame = +1 Query: 418 PNLR--RLQCGFCPRFCYSRRHNSFPFWSHFY 507 PN++ R++C P FC +R+ N +PF F+ Sbjct: 79 PNIKFMRVECPKYPGFCITRQKNEYPFIEIFH 110 >At5g61670.2 68418.m07738 expressed protein Length = 307 Score = 29.1 bits (62), Expect = 2.5 Identities = 19/74 (25%), Positives = 29/74 (39%), Gaps = 1/74 (1%) Frame = +2 Query: 344 RVCAGTGHRALTFCSSQGS-TFQAPAQIFVDSNVASVHVSATLVATTRSRSGVIFTETID 520 + C GTG+ A CSS G+ P N S+ T + S +G + T Sbjct: 231 KYCLGTGYLACARCSSTGALVLTEPVSAIAGGN-HSLSPPKTERCSNCSGAGKVMCPTCL 289 Query: 521 CSNQGLVRRHSLRL 562 C+ + H R+ Sbjct: 290 CTGMAMASEHDPRI 303 >At5g61670.1 68418.m07737 expressed protein Length = 307 Score = 29.1 bits (62), Expect = 2.5 Identities = 19/74 (25%), Positives = 29/74 (39%), Gaps = 1/74 (1%) Frame = +2 Query: 344 RVCAGTGHRALTFCSSQGS-TFQAPAQIFVDSNVASVHVSATLVATTRSRSGVIFTETID 520 + C GTG+ A CSS G+ P N S+ T + S +G + T Sbjct: 231 KYCLGTGYLACARCSSTGALVLTEPVSAIAGGN-HSLSPPKTERCSNCSGAGKVMCPTCL 289 Query: 521 CSNQGLVRRHSLRL 562 C+ + H R+ Sbjct: 290 CTGMAMASEHDPRI 303 >At5g58840.1 68418.m07373 subtilase family protein contains similarity to prepro-cucumisin GI:807698 from [Cucumis melo]; non-consensus acceptor site TT at exon 6 Length = 713 Score = 29.1 bits (62), Expect = 2.5 Identities = 26/106 (24%), Positives = 41/106 (38%), Gaps = 5/106 (4%) Frame = +2 Query: 287 RRDNPHKSYPILTSSQQTARVCAGTGHRALTFCSSQGSTFQAPAQIFVDSNVASVHVSAT 466 +RDN H Y +L+ + + AG TF + Q + + ++ + T Sbjct: 484 KRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSPSM---IQSAIMTTAWPMNATGT 540 Query: 467 LVATTRSRSGVIFTETIDCSNQGLVRR-----HSLRLCSMTNPSTS 589 VA+T G + I N GLV H LC + +TS Sbjct: 541 AVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLNYNATS 586 >At4g17460.1 68417.m02612 homeobox-leucine zipper protein 1 (HAT1) / HD-ZIP protein 1 identical to Homeobox-leucine zipper protein HAT1 (SP:P46600) [Arabidopsis thaliana] Length = 282 Score = 29.1 bits (62), Expect = 2.5 Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 3/38 (7%) Frame = -3 Query: 547 APYQALIGTIDGFRKNDSRTGTS---CGDESSRNVDRS 443 +P + T+ G R++ R GTS CGD+ +DRS Sbjct: 79 SPNSTISSTVSGKRRSTEREGTSGGGCGDDLDITLDRS 116 >At1g75210.1 68414.m08737 5' nucleotidase family protein contains Pfam profile: PF05761 5' nucleotidase family Length = 642 Score = 28.3 bits (60), Expect = 4.4 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = +3 Query: 147 SNYRLLSEVEHLKPYRDFYCHWTHRLPL 230 SN RL S ++ +P DF H H LPL Sbjct: 615 SNLRLYSPDKYYRPSEDFMSHEFHLLPL 642 >At5g10660.1 68418.m01234 calmodulin-binding protein-related contains weak similarity to calmodulin-binding proteins Length = 407 Score = 27.9 bits (59), Expect = 5.9 Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 2/30 (6%) Frame = +2 Query: 527 NQGLVRRHSLRL--CSMTNPSTSSTHLQMN 610 NQ L+RR S S+T+PSTSS H +N Sbjct: 78 NQKLLRRRSFDHPPSSLTSPSTSSAHRSLN 107 >At4g24750.1 68417.m03542 expressed protein Length = 260 Score = 27.9 bits (59), Expect = 5.9 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = -3 Query: 301 WIVSTSDKTFLRVRSRTPKNRARRRGSRWV 212 + +S S+K L VR + +N+A +GS WV Sbjct: 98 YAISLSNKPLLDVRPSSERNKAWIKGSTWV 127 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,918,709 Number of Sequences: 28952 Number of extensions: 284184 Number of successful extensions: 887 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 873 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 887 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1285411824 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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