BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060675.seq (634 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 48 1e-06 SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 46 3e-06 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 43 3e-05 SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 35 0.011 SPBC1921.03c |mex67||mRNA export receptor Mex67|Schizosaccharomy... 27 1.7 SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosacch... 25 6.9 SPAC56E4.03 |||aromatic aminotransferase |Schizosaccharomyces po... 25 6.9 SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 25 9.1 SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces pomb... 25 9.1 SPCC1529.01 ||SPCC794.14|membrane transporter|Schizosaccharomyce... 25 9.1 >SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 47.6 bits (108), Expect = 1e-06 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 1/51 (1%) Frame = +2 Query: 299 CAKCGEFV-IGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALCHV 448 C CG + GR+I A HP CF+C+ C+ L GF G+ CH+ Sbjct: 258 CHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308 Score = 47.6 bits (108), Expect = 1e-06 Identities = 18/66 (27%), Positives = 31/66 (46%) Frame = +2 Query: 260 YCEQDFQVLFAPCCAKCGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRAL 439 +C+ + +A C KC + ++G +K + +H C+ C CN L D G+ + Sbjct: 365 WCQTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPI 424 Query: 440 CHVCNA 457 C C A Sbjct: 425 CRPCKA 430 Score = 31.9 bits (69), Expect = 0.079 Identities = 29/113 (25%), Positives = 41/113 (36%), Gaps = 2/113 (1%) Frame = +2 Query: 251 GRKYCEQDFQVLFAPCCAKCGEFVIGRVIKAMNSNWHPACFRCEECN-VELADAGFIKHA 427 G+ YC D+ F+P C C + + + N +H C C+ V + I Sbjct: 302 GKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRD 361 Query: 428 GRALCHVCNARIKADGLQNYMCHKCHG*LTENLYVIGEKFTMG-YHFTCATCG 583 C C D C KC + + I K + G YH C TCG Sbjct: 362 DLYWCQTC-----YDNKYAVKCKKCR----KPILGISVKGSDGEYHSQCWTCG 405 Score = 31.1 bits (67), Expect = 0.14 Identities = 14/47 (29%), Positives = 22/47 (46%) Frame = +3 Query: 117 CTRCGDGFEPNEKIVNSNGELWHTNCFVCAQCFRMFPDGVFYEFEGE 257 C CG +I++++G+ H CF C C + FY EG+ Sbjct: 258 CHSCGGSLRAG-RIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGK 303 >SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1150 Score = 46.4 bits (105), Expect = 3e-06 Identities = 27/96 (28%), Positives = 44/96 (45%), Gaps = 2/96 (2%) Frame = +2 Query: 299 CAKCGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALCHVCNARIKADGL 478 CA CG+ + G+ ++A+ + +H CFRC +CN +A F V Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRR 175 Query: 479 QNYMCHKCHG*LTENLYV--IGEKFTMGYHFTCATC 580 + +C C G Y+ + +KF + HFTC+ C Sbjct: 176 LDLLCASC-GMALRGYYITALNKKFHI-EHFTCSLC 209 Score = 37.1 bits (82), Expect = 0.002 Identities = 19/62 (30%), Positives = 28/62 (45%), Gaps = 1/62 (1%) Frame = +2 Query: 263 CEQDFQVLFAPCCAKCGEFVIGRVIKAMNSNWHPACFRCEEC-NVELADAGFIKHAGRAL 439 CE D+ CA CG + G I A+N +H F C C V + + ++ G+ Sbjct: 168 CETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVY 227 Query: 440 CH 445 CH Sbjct: 228 CH 229 Score = 30.3 bits (65), Expect = 0.24 Identities = 12/44 (27%), Positives = 21/44 (47%), Gaps = 4/44 (9%) Frame = +2 Query: 251 GRKYCEQDFQVLFAPCCAKCGEFVIGRVIK----AMNSNWHPAC 370 G+ YC + LFA C C ++ + ++ ++ NWH C Sbjct: 224 GKVYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267 Score = 25.4 bits (53), Expect = 6.9 Identities = 10/32 (31%), Positives = 15/32 (46%) Frame = +3 Query: 117 CTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 212 C CG + + V + G ++H CF C C Sbjct: 116 CASCGQVI--SGQYVRALGNIYHLECFRCHDC 145 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 43.2 bits (97), Expect = 3e-05 Identities = 15/45 (33%), Positives = 24/45 (53%) Frame = +3 Query: 78 SLFLATMSLDNMYCTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 212 S+ + S + C RCG F+ E ++ G +WH +CF C +C Sbjct: 6 SISKSPSSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKC 50 Score = 34.3 bits (75), Expect = 0.015 Identities = 13/54 (24%), Positives = 24/54 (44%) Frame = +2 Query: 290 APCCAKCGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALCHVC 451 A C C + + + ++H CFRC +C ++ D+ F + C+ C Sbjct: 75 AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128 Score = 30.7 bits (66), Expect = 0.18 Identities = 27/87 (31%), Positives = 39/87 (44%), Gaps = 18/87 (20%) Frame = +2 Query: 299 CAKCGEFVIGRV--IKAMNSNWHPACFRCEECN--VELADAGFIKHA-GRALC----HVC 451 C +CG+ R I WH CF C +C+ +E +D ++ + GR +C H C Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSCAHTC 78 Query: 452 NA---RIK----ADGLQNY--MCHKCH 505 A RIK G +Y C +CH Sbjct: 79 TACRMRIKDYALMSGYDSYHRECFRCH 105 >SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosaccharomyces pombe|chr 2|||Manual Length = 933 Score = 34.7 bits (76), Expect = 0.011 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = +3 Query: 81 LFLATMSLDNMYCTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 212 L L T S +C +C + P+ V G+ WH++CF C C Sbjct: 12 LSLETPSERTCFCIKCWESV-PSTSQVWFGGKCWHSDCFKCVNC 54 Score = 28.3 bits (60), Expect = 0.97 Identities = 13/35 (37%), Positives = 15/35 (42%), Gaps = 1/35 (2%) Frame = +2 Query: 299 CAKCGEFVIGRVIKAMNSN-WHPACFRCEECNVEL 400 C KC E V WH CF+C CN +L Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKL 58 >SPBC1921.03c |mex67||mRNA export receptor Mex67|Schizosaccharomyces pombe|chr 2|||Manual Length = 596 Score = 27.5 bits (58), Expect = 1.7 Identities = 14/45 (31%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = -1 Query: 625 RTIFHFSGCMIQLNSTGSTGKVIAHGKL-LPDNVKVLRQLSMALM 494 R+ + F G + L S G+ K++ HG+ P N ++LR L+ Sbjct: 437 RSQWVFDGWNLVLPSVGAAIKIVVHGQFEEPQNKRLLRSFDRTLL 481 >SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 213 Score = 25.4 bits (53), Expect = 6.9 Identities = 12/34 (35%), Positives = 20/34 (58%), Gaps = 2/34 (5%) Frame = +3 Query: 90 ATMSLDNMYCTRCGDG--FEPNEKIVNSNGELWH 185 AT S+ N +CT+C D F ++I+ ++ E H Sbjct: 58 ATNSIINKFCTQCSDNDEFPTPKQIMETDVETLH 91 >SPAC56E4.03 |||aromatic aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.4 bits (53), Expect = 6.9 Identities = 12/43 (27%), Positives = 19/43 (44%) Frame = -2 Query: 294 GANKTWKSCSQYFLPQIHRRHHQETCESTGHKQNNLYAIILHW 166 G+ W + F+ + R T ++G Q LYA+ HW Sbjct: 302 GSRVGWFTAQPLFIERGLRAAETATQSASGISQGILYAMFKHW 344 >SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 334 Score = 25.0 bits (52), Expect = 9.1 Identities = 12/41 (29%), Positives = 20/41 (48%) Frame = -1 Query: 502 ALMTHVVLQPIRFDTRVTYMAQCSTSMLDKTGICKLYITLF 380 AL + L+ TR ++A+C T + IC+ Y + F Sbjct: 24 ALGKYAELKTYSDGTREDFLAKCKTEFQNVKAICRTYNSKF 64 >SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces pombe|chr 2|||Manual Length = 827 Score = 25.0 bits (52), Expect = 9.1 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = -1 Query: 565 KVIAHGKLLPDNVKVLRQLSMALMTHVV 482 + + H KL PDN L S+ L+ H++ Sbjct: 439 RTLHHVKLHPDNKSRLENFSVILLQHIL 466 >SPCC1529.01 ||SPCC794.14|membrane transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 462 Score = 25.0 bits (52), Expect = 9.1 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 4/54 (7%) Frame = -1 Query: 505 MALMTHVVLQPIRFDTRVTYMAQCSTSMLDKTGICKL----YITLFTSEAGRMP 356 + L+T +++PIR + CST M + GI L Y TL+T E P Sbjct: 259 LKLVTPALMRPIRMLFTQPIVILCSTYMAIQYGILYLVLTTYPTLWTEEYHERP 312 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,879,218 Number of Sequences: 5004 Number of extensions: 64298 Number of successful extensions: 185 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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