Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060675.seq
(634 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 42 3e-04
At5g66610.1 68418.m08396 LIM domain-containing protein contains ... 37 0.013
At5g66630.1 68418.m08398 LIM domain-containing protein contains ... 32 0.28
At5g17890.1 68418.m02098 LIM domain-containing protein / disease... 32 0.36
At4g36860.1 68417.m05226 LIM domain-containing protein low simil... 32 0.36
At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to ac... 31 0.84
At2g39830.1 68415.m04892 LIM domain-containing protein contains ... 31 0.84
At1g10200.1 68414.m01150 transcription factor LIM, putative stro... 29 1.9
At5g66620.1 68418.m08397 LIM domain-containing protein contains ... 29 2.6
At5g01760.1 68418.m00095 VHS domain-containing protein / GAT dom... 29 2.6
At2g46440.1 68415.m05779 cyclic nucleotide-regulated ion channel... 29 2.6
At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong simil... 29 2.6
At3g45530.1 68416.m04917 DC1 domain-containing protein contains ... 29 3.4
At2g39900.1 68415.m04904 LIM domain-containing protein similar t... 29 3.4
At1g25250.1 68414.m03133 zinc finger (C2H2 type) family protein ... 29 3.4
At4g39370.2 68417.m05572 ubiquitin-specific protease 27, putativ... 28 4.5
At4g39370.1 68417.m05573 ubiquitin-specific protease 27, putativ... 28 4.5
At5g29624.1 68418.m03640 DC1 domain-containing protein contains ... 28 5.9
At5g22620.1 68418.m02643 phosphoglycerate/bisphosphoglycerate mu... 28 5.9
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 28 5.9
At3g61230.1 68416.m06852 LIM domain-containing protein similar t... 27 7.8
At2g24800.1 68415.m02967 peroxidase, putative similar to peroxid... 27 7.8
At1g03630.1 68414.m00343 protochlorophyllide reductase C, chloro... 27 7.8
At1g01780.1 68414.m00097 LIM domain-containing protein similar t... 27 7.8
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 41.9 bits (94), Expect = 3e-04
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = +2
Query: 275 FQVLFAPCCAKCGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGF 415
FQ+ F C E GR + +NS WHP CFRC C+ +++ F
Sbjct: 165 FQMDFRICAGCNMEIGHGRFLNCLNSLWHPECFRCYGCSQPISEYEF 211
Score = 27.9 bits (59), Expect = 5.9
Identities = 15/52 (28%), Positives = 21/52 (40%)
Frame = +3
Query: 93 TMSLDNMYCTRCGDGFEPNEKIVNSNGELWHTNCFVCAQCFRMFPDGVFYEF 248
T +D C C + + +N LWH CF C C + + YEF
Sbjct: 164 TFQMDFRICAGCNMEIG-HGRFLNCLNSLWHPECFRCYGCSQPISE---YEF 211
>At5g66610.1 68418.m08396 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 529
Score = 36.7 bits (81), Expect = 0.013
Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = +2
Query: 299 CAKCGEFV-IGRVIKAMNSNWHPACFRCEECNVELADAGFIKH 424
C C + GR + A+ NWHP CF C C+ +A + +H
Sbjct: 201 CDGCKSAIEYGRSVHALGVNWHPECFCCRYCDKPIAMHEYKEH 243
>At5g66630.1 68418.m08398 LIM domain-containing protein contains low
similarity to Pfam profile PF00412: LIM domain
Length = 702
Score = 32.3 bits (70), Expect = 0.28
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +3
Query: 117 CTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 212
C C + +E+ VN G LWH CF C C
Sbjct: 347 CGGCNSAVK-HEESVNILGVLWHPGCFCCRSC 377
Score = 28.3 bits (60), Expect = 4.5
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2
Query: 356 WHPACFRCEECNVELA 403
WHP CF C C+ +A
Sbjct: 367 WHPGCFCCRSCDKPIA 382
>At5g17890.1 68418.m02098 LIM domain-containing protein / disease
resistance protein-related low similarity to disease
resistance protein RPP4 [Arabidopsis thaliana]
GI:20270890; contains Pfam profiles PF00412: LIM domain,
PF00931: NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1613
Score = 31.9 bits (69), Expect = 0.36
Identities = 12/32 (37%), Positives = 15/32 (46%)
Frame = +3
Query: 117 CTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 212
C C E I N+ G +WH CF C +C
Sbjct: 1240 CKDCKSAIEDGISI-NAYGSVWHPQCFCCLRC 1270
Score = 30.3 bits (65), Expect = 1.1
Identities = 14/36 (38%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +2
Query: 299 CAKCGEFVI-GRVIKAMNSNWHPACFRCEECNVELA 403
C C + G I A S WHP CF C C +A
Sbjct: 1240 CKDCKSAIEDGISINAYGSVWHPQCFCCLRCREPIA 1275
>At4g36860.1 68417.m05226 LIM domain-containing protein low
similarity to LIM-domain protein [Branchiostoma
floridae] GI:3360516, DRAL/Slim3/FHL2 [Homo sapiens]
GI:7209525; contains Pfam profile PF00412: LIM domain
Length = 351
Score = 31.9 bits (69), Expect = 0.36
Identities = 16/52 (30%), Positives = 21/52 (40%), Gaps = 3/52 (5%)
Frame = +2
Query: 344 MNSNWHPACFRCEECNVELADAGFIKHAGR---ALCHVCNARIKADGLQNYM 490
M WHP CF C C+ + D F R LC+ K D N++
Sbjct: 1 MGGVWHPECFCCNACDKPIIDYEFSMSGNRPYHKLCYKEQHHPKCDVCHNFI 52
>At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to
acyl-CoA oxidase [Arabidopsis thaliana] GI:3044214
Length = 675
Score = 30.7 bits (66), Expect = 0.84
Identities = 21/88 (23%), Positives = 37/88 (42%)
Frame = +3
Query: 42 LIKTRFFSLPNASLFLATMSLDNMYCTRCGDGFEPNEKIVNSNGELWHTNCFVCAQCFRM 221
L + F +LP A T L ++ T DG E K+ +G LW C + F +
Sbjct: 362 LAASDFATLPEAHA--CTAGLKSLTTTATADGIEECRKLCGGHGYLW---CSGLPELFAV 416
Query: 222 FPDGVFYEFEGESIVNRISKFYLHHAVQ 305
+ YE + + ++++F + Q
Sbjct: 417 YVPACTYEGDNVVLQLQVARFLMKTVAQ 444
>At2g39830.1 68415.m04892 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 503
Score = 30.7 bits (66), Expect = 0.84
Identities = 11/37 (29%), Positives = 17/37 (45%), Gaps = 1/37 (2%)
Frame = +2
Query: 299 CAKCGEFV-IGRVIKAMNSNWHPACFRCEECNVELAD 406
C C + G + M + +HP CFRC C + +
Sbjct: 162 CGGCNSDIGSGNYLGCMGTFFHPECFRCHSCGYAITE 198
>At1g10200.1 68414.m01150 transcription factor LIM, putative strong
similarity to transcription factor Ntlim1 [Nicotiana
tabacum] GI:5689136, LIM domain protein WLIM-1
[Helianthus annuus] GI:5070280; contains Pfam profile
PF00412: LIM domain
Length = 190
Score = 29.5 bits (63), Expect = 1.9
Identities = 12/49 (24%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Frame = +2
Query: 299 CAKCGEFVIGRVIKAMNSN-WHPACFRCEECNVELADAGFIKHAGRALC 442
C C + V ++N +H +CF+C ++ + +I H G+ C
Sbjct: 110 CVGCDKTVYPIEKVSVNGTLYHKSCFKCTHGGCTISPSNYIAHEGKLYC 158
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +3
Query: 117 CTRCGDGFEPNEKIVNSNGELWHTNCFVC 203
C C P EK V+ NG L+H +CF C
Sbjct: 110 CVGCDKTVYPIEK-VSVNGTLYHKSCFKC 137
Score = 28.7 bits (61), Expect = 3.4
Identities = 15/49 (30%), Positives = 21/49 (42%), Gaps = 1/49 (2%)
Frame = +2
Query: 299 CAKCGEFV-IGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALC 442
C C + V + + A N +H ACFRC C L + + G C
Sbjct: 10 CMACDKTVYLVDKLTADNRVYHKACFRCHHCKGTLKLSNYNSFEGVLYC 58
>At5g66620.1 68418.m08397 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 644
Score = 29.1 bits (62), Expect = 2.6
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +3
Query: 159 VNSNGELWHTNCFVCAQCFR 218
VN G LWH CF C C +
Sbjct: 299 VNILGVLWHPGCFCCRACHK 318
Score = 28.7 bits (61), Expect = 3.4
Identities = 12/36 (33%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +2
Query: 299 CAKCGEFVI-GRVIKAMNSNWHPACFRCEECNVELA 403
C C V G + + WHP CF C C+ +A
Sbjct: 286 CGGCNFAVEHGGSVNILGVLWHPGCFCCRACHKPIA 321
>At5g01760.1 68418.m00095 VHS domain-containing protein / GAT
domain-containing protein weak similarity to Hrs [Rattus
norvegicus] GI:8547026; contains Pfam profiles PF00790:
VHS domain, PF03127: GAT domain
Length = 542
Score = 29.1 bits (62), Expect = 2.6
Identities = 20/65 (30%), Positives = 28/65 (43%), Gaps = 6/65 (9%)
Frame = -2
Query: 276 KSCSQYFLPQIHRRHHQETCESTGHKQ------NNLYAIILHWSSQFSHLAQIHPHSEYN 115
K+C + I +H E K+ N L ++ W+ FS +A HPH YN
Sbjct: 87 KNCGDFVHSHIAEKHLLEDMVKLVRKKGDFEVRNKLLILLDTWNEAFSGVACKHPH--YN 144
Query: 114 TYYQE 100
YQE
Sbjct: 145 WAYQE 149
>At2g46440.1 68415.m05779 cyclic nucleotide-regulated ion channel,
putative (CNGC11) similar to cyclic nucleotide and
calmodulin-regulated ion channel (cngc3) GI:4581201 from
[Arabidopsis thaliana]
Length = 588
Score = 29.1 bits (62), Expect = 2.6
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +2
Query: 308 CGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAG 430
CG F I+ ++ W AC R +CN+ + D K AG
Sbjct: 182 CGAFWYLSSIERKSTCWRAACARTSDCNLTVTDL-LCKRAG 221
>At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong
similarity to acyl-CoA oxidase [Arabidopsis thaliana]
GI:3044214
Length = 664
Score = 29.1 bits (62), Expect = 2.6
Identities = 19/78 (24%), Positives = 34/78 (43%)
Frame = +3
Query: 57 FFSLPNASLFLATMSLDNMYCTRCGDGFEPNEKIVNSNGELWHTNCFVCAQCFRMFPDGV 236
F +LP A T L +M + DG E K+ +G LW C + F ++
Sbjct: 367 FATLPEAHA--CTAGLKSMTTSATSDGIEECRKLCGGHGYLW---CSGLPELFAVYVPAC 421
Query: 237 FYEFEGESIVNRISKFYL 290
YE + + ++++F +
Sbjct: 422 TYEGDNVVLQLQVARFLM 439
>At3g45530.1 68416.m04917 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 692
Score = 28.7 bits (61), Expect = 3.4
Identities = 12/36 (33%), Positives = 22/36 (61%), Gaps = 3/36 (8%)
Frame = +3
Query: 195 FVCAQCFRMFPDGVFYEFEG---ESIVNRISKFYLH 293
F+C C++MF +G FY +G + I + I+ ++H
Sbjct: 447 FLCRACYQMFCEGFFYSSKGVNFDLICSSITMPFIH 482
>At2g39900.1 68415.m04904 LIM domain-containing protein similar to
pollen specific LIM domain protein 1b [Nicotiana
tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
GI:4105772; contains Pfam profile PF00412: LIM domain
Length = 200
Score = 28.7 bits (61), Expect = 3.4
Identities = 13/49 (26%), Positives = 21/49 (42%), Gaps = 1/49 (2%)
Frame = +2
Query: 299 CAKCGEFVIG-RVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALC 442
C C + V ++ A ++H ACF+C C L + + G C
Sbjct: 10 CRACEKTVYPVELLSADGISYHKACFKCSHCKSRLQLSNYSSMEGVVYC 58
>At1g25250.1 68414.m03133 zinc finger (C2H2 type) family protein
contains zinc finger, C2H2 type, domain, PROSITE:PS00028
Length = 362
Score = 28.7 bits (61), Expect = 3.4
Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = -2
Query: 309 HFAQ-HGANKTW--KSCSQYFLPQIHRRHHQETCESTGH 202
HF + H +K W + CS+ + Q + H +TC S GH
Sbjct: 107 HFRRKHSVHKQWVCERCSKGYAVQSDYKAHLKTCGSRGH 145
>At4g39370.2 68417.m05572 ubiquitin-specific protease 27, putative
(UBP27) similar to GI:11993494; ubiquitin specific
protease 66 - Gallus gallus,PID:g3800764
Length = 361
Score = 28.3 bits (60), Expect = 4.5
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 398 LADAGFIKHAGRALCHVCNARIKADGLQNYMCHKC-HG*LTENLYVIG 538
L G+ +G L H + + ++NY C++C HG + L VIG
Sbjct: 263 LHHGGYNIMSGCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIG 310
>At4g39370.1 68417.m05573 ubiquitin-specific protease 27, putative
(UBP27) similar to GI:11993494; ubiquitin specific
protease 66 - Gallus gallus,PID:g3800764
Length = 494
Score = 28.3 bits (60), Expect = 4.5
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 398 LADAGFIKHAGRALCHVCNARIKADGLQNYMCHKC-HG*LTENLYVIG 538
L G+ +G L H + + ++NY C++C HG + L VIG
Sbjct: 263 LHHGGYNIMSGCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIG 310
>At5g29624.1 68418.m03640 DC1 domain-containing protein contains
Pfam PF03107: DC1 domain
Length = 488
Score = 27.9 bits (59), Expect = 5.9
Identities = 29/113 (25%), Positives = 45/113 (39%), Gaps = 13/113 (11%)
Frame = +2
Query: 299 CAKCGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALCHVCNARIKADGL 478
C +CG + + IK + NW C N+ + CH C +RIK+
Sbjct: 64 CTECGLNMHKKCIKRLFLNWPFLCNHV--LNIFQNTFTSSDNIDDFSCHFCQSRIKSSFA 121
Query: 479 QNYMCH-----KCHG*LTENLYVI---GEKFTMG-----YHFTCATCGVELDH 598
+ +C+ KC L L + K+++ F C+ CGVE DH
Sbjct: 122 RFTLCNINVDEKCMVELQPPLKISEPKHHKYSLTILLWLVTFICSPCGVEGDH 174
>At5g22620.1 68418.m02643 phosphoglycerate/bisphosphoglycerate
mutase family protein weak similarity to SP|P15259
Phosphoglycerate mutase, muscle form (EC 5.4.2.1 {Homo
sapiens}; contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 482
Score = 27.9 bits (59), Expect = 5.9
Identities = 16/54 (29%), Positives = 23/54 (42%)
Frame = -2
Query: 441 HNARPACLIKPASASSTLHSSHRKQAGCQLEFIAFITRPITNSPHFAQHGANKT 280
HNA L+ A T + Q+ C + + FI R SPH + N+T
Sbjct: 196 HNAVNQALLATAIGLGTEYFRSLLQSNCGVSVLDFIPRADGGSPHVCLNRLNQT 249
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 27.9 bits (59), Expect = 5.9
Identities = 13/49 (26%), Positives = 20/49 (40%), Gaps = 1/49 (2%)
Frame = +2
Query: 299 CAKCGEFVIGRVIKAMNSN-WHPACFRCEECNVELADAGFIKHAGRALC 442
C C + V + + N +H +CFRC C L + + G C
Sbjct: 10 CKACDKTVYVMDLLTLEGNTYHKSCFRCTHCKGTLVISNYSSMDGVLYC 58
>At3g61230.1 68416.m06852 LIM domain-containing protein similar to
SP|P29675 Pollen specific protein SF3 {Helianthus
annuus}; contains Pfam profile PF00412: LIM domain
Length = 213
Score = 27.5 bits (58), Expect = 7.8
Identities = 10/29 (34%), Positives = 13/29 (44%)
Frame = +2
Query: 356 WHPACFRCEECNVELADAGFIKHAGRALC 442
+H +CFRC CN L + G C
Sbjct: 31 YHKSCFRCSHCNGTLVICNYSSMDGVLYC 59
>At2g24800.1 68415.m02967 peroxidase, putative similar to peroxidase
[Spinacia oleracea] gi|1781338|emb|CAA71496
Length = 329
Score = 27.5 bits (58), Expect = 7.8
Identities = 15/50 (30%), Positives = 23/50 (46%), Gaps = 4/50 (8%)
Frame = +3
Query: 39 NLIKTRFFSLPNASLFLATMSLDNMYC----TRCGDGFEPNEKIVNSNGE 176
N +RF P +L L SLDN Y +C +P +V+++ E
Sbjct: 202 NTFNSRFKLDPKGNLELIDASLDNSYAQTLVNKCSSSLDPTTTVVDNDPE 251
>At1g03630.1 68414.m00343 protochlorophyllide reductase C,
chloroplast / PCR C / NADPH-protochlorophyllide
oxidoreductase C (PORC) identical to SP:O48741
protochlorophyllide reductase C, chloroplast precursor
(EC 1.3.1.33) (PCR C) (NADPH-protochlorophyllide
oxidoreductase C) (POR C) [Arabidopsis thaliana]
Length = 401
Score = 27.5 bits (58), Expect = 7.8
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -2
Query: 291 ANKTWKSCSQYFLPQIHRRHHQET 220
A K K C+ + ++HRR+H+ET
Sbjct: 275 AYKDSKVCNMLTMQELHRRYHEET 298
>At1g01780.1 68414.m00097 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 205
Score = 27.5 bits (58), Expect = 7.8
Identities = 13/30 (43%), Positives = 14/30 (46%)
Frame = +3
Query: 117 CTRCGDGFEPNEKIVNSNGELWHTNCFVCA 206
C C P EKI GE +H CF CA
Sbjct: 104 CAACEKTVYPLEKI-QMEGECFHKTCFRCA 132
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,917,004
Number of Sequences: 28952
Number of extensions: 334865
Number of successful extensions: 961
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 961
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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