Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060671.seq
(688 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 165 4e-42
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 158 8e-40
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 141 7e-35
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 134 1e-32
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 105 5e-24
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 51 1e-07
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 1e-04
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.039
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.83
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 5.9
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.8
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 25 7.8
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 165 bits (402), Expect = 4e-42
Identities = 87/142 (61%), Positives = 104/142 (73%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435
NP+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SS
Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119
Query: 436 MVLTKMKETAEAYLGKTVQNAVITVPRTSMTLKDKPQKMQVPSSGLNVSSEFINEPTAAA 615
MVL KM+ETAEAYLG V +AV+TVP + + K +GLNV INEPTAAA
Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNV-LRIINEPTAAA 178
Query: 616 IAYGL*QKRYLENEMYLSFDLG 681
IAYGL + E+ + L FDLG
Sbjct: 179 IAYGLDRSNQGESNV-LIFDLG 199
Score = 107 bits (257), Expect = 1e-24
Identities = 49/58 (84%), Positives = 53/58 (91%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 158 bits (383), Expect = 8e-40
Identities = 83/142 (58%), Positives = 101/142 (71%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435
NP+NTIFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SS
Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119
Query: 436 MVLTKMKETAEAYLGKTVQNAVITVPRTSMTLKDKPQKMQVPSSGLNVSSEFINEPTAAA 615
MVL KM+E+AEA+LG V +AV+TVP + + K +GLNV INEPTAAA
Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNV-LRIINEPTAAA 178
Query: 616 IAYGL*QKRYLENEMYLSFDLG 681
IAYGL + E + L FDLG
Sbjct: 179 IAYGLDRSNQHETNV-LIFDLG 199
Score = 107 bits (257), Expect = 1e-24
Identities = 49/58 (84%), Positives = 53/58 (91%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 141 bits (342), Expect = 7e-35
Identities = 77/142 (54%), Positives = 92/142 (64%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435
NP NT+FDAKRLIGR++ED Q D+KHWPF+V+ G P I+V Y GE K F +E+S+
Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISA 123
Query: 436 MVLTKMKETAEAYLGKTVQNAVITVPRTSMTLKDKPQKMQVPSSGLNVSSEFINEPTAAA 615
MVLTKMKE +EA L K V+ AVITVP + K +GLNV INEPTAAA
Sbjct: 124 MVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNV-LRIINEPTAAA 182
Query: 616 IAYGL*QKRYLENEMYLSFDLG 681
IAYGL K + L FDLG
Sbjct: 183 IAYGLDAKSDKPKNV-LIFDLG 203
Score = 95.5 bits (227), Expect = 6e-21
Identities = 43/58 (74%), Positives = 49/58 (84%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A P
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 134 bits (323), Expect = 1e-32
Identities = 71/142 (50%), Positives = 96/142 (67%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435
NP NTIFD KRLIGRKF++ T+ D+K +PF +V+ +P ++V G+ K F PEE+S+
Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152
Query: 436 MVLTKMKETAEAYLGKTVQNAVITVPRTSMTLKDKPQKMQVPSSGLNVSSEFINEPTAAA 615
M+L+KMK+TAEAYLGK V +AV+TVP + + K +GLNV +NEPTAAA
Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNV-IRIVNEPTAAA 211
Query: 616 IAYGL*QKRYLENEMYLSFDLG 681
IAYGL K E + + +DLG
Sbjct: 212 IAYGL-DKTDTEKHIVV-YDLG 231
Score = 95.9 bits (228), Expect = 5e-21
Identities = 42/57 (73%), Positives = 49/57 (85%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNP 94
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 105 bits (253), Expect = 5e-24
Identities = 59/142 (41%), Positives = 83/142 (58%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435
NP NT F KRLIGR+F++ VQ D+K P+++V + KT+ P ++
Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGG 165
Query: 436 MVLTKMKETAEAYLGKTVQNAVITVPRTSMTLKDKPQKMQVPSSGLNVSSEFINEPTAAA 615
+L+KM+ETA YLGK V+NAV+TVP + + K +GLNV +NEPTAAA
Sbjct: 166 FILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNV-LRVVNEPTAAA 224
Query: 616 IAYGL*QKRYLENEMYLSFDLG 681
+AYGL +K + + FDLG
Sbjct: 225 LAYGLDKK---NDAIVAVFDLG 243
Score = 69.3 bits (162), Expect = 5e-13
Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
Frame = +2
Query: 80 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAR 256
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 257 TPTTQY 274
P +
Sbjct: 109 NPENTF 114
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 51.2 bits (117), Expect = 1e-07
Identities = 35/126 (27%), Positives = 60/126 (47%), Gaps = 1/126 (0%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVS 432
N NT+ KRL GR ++D ++ + ++ G KV Y E+ F ++
Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122
Query: 433 SMVLTKMKETAEAYLGKTVQNAVITVPRTSMTLKDKPQKMQVPSSGLNVSSEFINEPTAA 612
+ TK+K AEA L +V + VI++P ++ + +GLN +N+ AA
Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANIAGLN-PLRLMNDNAAA 181
Query: 613 AIAYGL 630
A+ YG+
Sbjct: 182 ALTYGI 187
Score = 49.2 bits (112), Expect = 6e-07
Identities = 20/59 (33%), Positives = 38/59 (64%)
Frame = +2
Query: 77 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 41.5 bits (93), Expect = 1e-04
Identities = 17/55 (30%), Positives = 31/55 (56%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAR 256
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ R
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVR 81
Score = 37.5 bits (83), Expect = 0.002
Identities = 30/128 (23%), Positives = 60/128 (46%), Gaps = 6/128 (4%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGED------KTFF 417
N +N++ + + L+G+ ++ T+ H+ V+ G+ K+ + ++ K
Sbjct: 82 NADNSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDEESDPKEKILT 139
Query: 418 PEEVSSMVLTKMKETAEAYLGKTVQNAVITVPRTSMTLKDKPQKMQVPSSGLNVSSEFIN 597
E S L ++ E+AE +LG V V++VP + K + +GL V + I+
Sbjct: 140 AHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGLPV-LQLIH 198
Query: 598 EPTAAAIA 621
+P A +A
Sbjct: 199 DPAAVILA 206
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 33.1 bits (72), Expect = 0.039
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTPTT 268
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N R P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83
Query: 269 QYSMPNV 289
+S+ NV
Sbjct: 84 -HSIRNV 89
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.83
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 84 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 179
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.8 bits (54), Expect = 5.9
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = -3
Query: 170 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 12
G W SP S + H ++++ +F +RVL C+ L K +K+A L
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 200 AFTDTERLIGDAAKNQVARTPTTQYSMPNVSSDV 301
AF D + G AK A P+T+ + N+S V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188
>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 570
Score = 25.4 bits (53), Expect = 7.8
Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = -1
Query: 262 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 113
WGS +G + TLG+ + + + L+VGD LHL V ++ +A +R
Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,029,965
Number of Sequences: 5004
Number of extensions: 65678
Number of successful extensions: 233
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -