BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060667.seq (635 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 97 9e-21 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 94 6e-20 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 89 2e-18 At2g05350.1 68415.m00563 hypothetical protein 29 2.0 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 97.1 bits (231), Expect = 9e-21 Identities = 41/72 (56%), Positives = 52/72 (72%) Frame = +2 Query: 56 EDTHFETGDSGXSXTFPMQCSXLRKNGFXMLXGRPCKIVEMSTSKTGKHGHAKVHLVGIX 235 E+ HFE+ D+G S T+P Q +RKNG+ ++ RPCK+VE+STSKTGKHGHAK H V I Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 236 XFNGKKLKISVP 271 F KKL+ VP Sbjct: 64 IFTSKKLEDIVP 75 Score = 81.8 bits (193), Expect = 3e-16 Identities = 38/72 (52%), Positives = 51/72 (70%), Gaps = 1/72 (1%) Frame = +1 Query: 250 KVEDICPSTHXMXVPHVKREDYQLTDISDDGYLXLXADNGDLR*DLKIPDGD-LGTXLRT 426 K+EDI PS+H VPHV R DYQL DIS+DGY+ L DNG + DLK+P+ D L +++ Sbjct: 69 KLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIKS 128 Query: 427 DFDSGKELLCTV 462 FD GK+L+ +V Sbjct: 129 GFDDGKDLVVSV 140 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 94.3 bits (224), Expect = 6e-20 Identities = 40/72 (55%), Positives = 51/72 (70%) Frame = +2 Query: 56 EDTHFETGDSGXSXTFPMQCSXLRKNGFXMLXGRPCKIVEMSTSKTGKHGHAKVHLVGIX 235 ++ HFE+ D+G S T+P Q +RK G ++ GRPCK+VE+STSKTGKHGHAK H V I Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 236 XFNGKKLKISVP 271 F KKL+ VP Sbjct: 64 IFTSKKLEDIVP 75 Score = 78.6 bits (185), Expect = 3e-15 Identities = 37/72 (51%), Positives = 50/72 (69%), Gaps = 1/72 (1%) Frame = +1 Query: 250 KVEDICPSTHXMXVPHVKREDYQLTDISDDGYLXLXADNGDLR*DLKIP-DGDLGTXLRT 426 K+EDI PS+H VPHV R DYQL DIS+DG++ L DNG + DLK+P D L T L+ Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLKN 128 Query: 427 DFDSGKELLCTV 462 F+ GK+++ +V Sbjct: 129 GFEEGKDIVVSV 140 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 89.0 bits (211), Expect = 2e-18 Identities = 38/72 (52%), Positives = 48/72 (66%) Frame = +2 Query: 56 EDTHFETGDSGXSXTFPMQCSXLRKNGFXMLXGRPCKIVEMSTSKTGKHGHAKVHLVGIX 235 ++ HFE +SG S T+P +RK G ++ RPCK+VE+STSKTGKHGHAK H V I Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 236 XFNGKKLKISVP 271 F KKL+ VP Sbjct: 64 IFTAKKLEDIVP 75 Score = 76.2 bits (179), Expect = 2e-14 Identities = 35/72 (48%), Positives = 49/72 (68%), Gaps = 1/72 (1%) Frame = +1 Query: 250 KVEDICPSTHXMXVPHVKREDYQLTDISDDGYLXLXADNGDLR*DLKIP-DGDLGTXLRT 426 K+EDI PS+H VPHV R DYQL DI++DG++ L D+G + DLK+P D L +R Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRL 128 Query: 427 DFDSGKELLCTV 462 FD GK+++ +V Sbjct: 129 GFDEGKDIVVSV 140 >At2g05350.1 68415.m00563 hypothetical protein Length = 453 Score = 29.5 bits (63), Expect = 2.0 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +2 Query: 383 TSKFRMVTSAHXCVLTSTAARNCCAPWLKSCGEE 484 TS+ RMV + C+ +T A NC + K G+E Sbjct: 110 TSQLRMVFAQVPCICFNTIAYNCPGTFFKQLGQE 143 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,421,585 Number of Sequences: 28952 Number of extensions: 229007 Number of successful extensions: 455 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 441 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 454 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1305036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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