Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060666.seq
(687 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 190 2e-49
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 114 1e-26
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 105 6e-24
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 104 1e-23
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 68 1e-12
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 63 3e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 52 1e-07
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 39 6e-04
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.36
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.36
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.63
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 27 1.9
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.4
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 4.4
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 5.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 190 bits (462), Expect = 2e-49
Identities = 89/108 (82%), Positives = 92/108 (85%)
Frame = +3
Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANRE 434
AQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE PLNPK+NRE
Sbjct: 58 AQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNRE 117
Query: 435 KMTQIMFETFNTPAMYVAIQAVLSLTRPXVPXVFVLDSGDGVSHNVXI 578
KMTQI+FETFN PA YVAIQAVLSL VLDSGDGV+H V I
Sbjct: 118 KMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPI 165
Score = 119 bits (287), Expect = 3e-28
Identities = 52/60 (86%), Positives = 58/60 (96%)
Frame = +1
Query: 88 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEHRAK 267
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDE ++K
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Score = 43.2 bits (97), Expect = 4e-05
Identities = 27/59 (45%), Positives = 33/59 (55%)
Frame = +2
Query: 509 YASGRTXGIRAGLRRRCLPQRAHLTKDYAXPHAILRLDLSRSRTSQXXFMKILXERGYS 685
YASGRT GI + + + YA PHAI+RLDL+ R MKIL ERGY+
Sbjct: 143 YASGRTTGIVLDSGDG-VTHTVPIYEGYALPHAIMRLDLA-GRDLTDYLMKILMERGYT 199
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 114 bits (275), Expect = 1e-26
Identities = 50/112 (44%), Positives = 78/112 (69%)
Frame = +3
Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANRE 434
A++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANRE
Sbjct: 58 AEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANRE 117
Query: 435 KMTQIMFETFNTPAMYVAIQAVLSLTRPXVPXVFVLDSGDGVSHNVXILRRI 590
KM + MFE + +YVAIQAVLSL + V+DSGDGV+H V + +
Sbjct: 118 KMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169
Score = 52.4 bits (120), Expect = 6e-08
Identities = 29/59 (49%), Positives = 36/59 (61%), Gaps = 2/59 (3%)
Frame = +1
Query: 94 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEHRA 264
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDE A
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 105 bits (252), Expect = 6e-24
Identities = 49/109 (44%), Positives = 72/109 (66%), Gaps = 1/109 (0%)
Frame = +3
Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEXPLNPKANR 431
AQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+
Sbjct: 62 AQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNK 121
Query: 432 EKMTQIMFETFNTPAMYVAIQAVLSLTRPXVPXVFVLDSGDGVSHNVXI 578
EK+ + +ET N PA+ ++Q VL+L VL+ GDG++H+V I
Sbjct: 122 EKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPI 170
Score = 65.7 bits (153), Expect = 6e-12
Identities = 28/50 (56%), Positives = 36/50 (72%)
Frame = +1
Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 255
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG E
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSE 61
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 104 bits (250), Expect = 1e-23
Identities = 49/108 (45%), Positives = 65/108 (60%), Gaps = 8/108 (7%)
Frame = +3
Query: 279 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQIMFE 458
+L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE
Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148
Query: 459 TFNTPAMYVAIQAVLSLTRPXVPXVF--------VLDSGDGVSHNVXI 578
+FN +Y+A+QAVL+L V+DSGDGV+H + +
Sbjct: 149 SFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPV 196
Score = 42.7 bits (96), Expect = 5e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +1
Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 255
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 67.7 bits (158), Expect = 1e-12
Identities = 34/100 (34%), Positives = 57/100 (57%), Gaps = 3/100 (3%)
Frame = +3
Query: 291 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQIMFETFNT 470
P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 471 PAMYVAIQAVLSL---TRPXVPXVFVLDSGDGVSHNVXIL 581
P++ I + S T+P + VL+ G+ SH + +L
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGI-VLNLGNAASHVIPVL 183
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 201
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 63.3 bits (147), Expect = 3e-11
Identities = 29/73 (39%), Positives = 41/73 (56%)
Frame = +3
Query: 276 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQIMF 455
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 456 ETFNTPAMYVAIQ 494
E+ PA Y+A Q
Sbjct: 123 ESLRCPATYLAKQ 135
Score = 40.3 bits (90), Expect = 3e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +1
Query: 91 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 192
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 51.6 bits (118), Expect = 1e-07
Identities = 21/67 (31%), Positives = 39/67 (58%)
Frame = +3
Query: 300 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQIMFETFNTPAM 479
+GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 480 YVAIQAV 500
Y+ QAV
Sbjct: 134 YLTKQAV 140
Score = 37.1 bits (82), Expect = 0.002
Identities = 19/55 (34%), Positives = 32/55 (58%)
Frame = +1
Query: 91 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 255
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDE 56
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 39.1 bits (87), Expect = 6e-04
Identities = 25/91 (27%), Positives = 40/91 (43%), Gaps = 4/91 (4%)
Frame = +3
Query: 291 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEXPLNPKANREKMTQIMFE 458
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 459 TFNTPAMYVAIQAVLSLTRPXVPXVFVLDSG 551
P +A + ++ L + V+D G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.9 bits (64), Expect = 0.36
Identities = 18/53 (33%), Positives = 30/53 (56%), Gaps = 5/53 (9%)
Frame = +3
Query: 351 FYNELRVAPEEHPVLLTE--XPLNPKANREKMTQIMFETFNTPAM---YVAIQ 494
+Y L E+HP+LLT+ L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.9 bits (64), Expect = 0.36
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -2
Query: 248 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 87
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 29.1 bits (62), Expect = 0.63
Identities = 18/66 (27%), Positives = 30/66 (45%)
Frame = -2
Query: 323 IPVSDDSVFDGVFEGQDTSFALCSSPT*ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 144
+P + +V +TSFAL ++ S PT + P +E + +S+PA
Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122
Query: 143 PALHIP 126
P L+ P
Sbjct: 123 PQLNSP 128
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = -3
Query: 250 LLHKSPSVPYRPSRPDGGAFPRSRGTR 170
L+ K+PS PY SRP A S TR
Sbjct: 309 LISKAPSYPYSSSRPSASASLASSPTR 335
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 3.4
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -2
Query: 251 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 135
S T +SF P+PT P LPT NT + P+ PAL
Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 26.2 bits (55), Expect = 4.4
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +1
Query: 151 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 255
G+ PRA F ++ P H G+++ M KD G+E
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -3
Query: 133 TYRSHCLLPTRQLLRRTS*ISEI 65
TYR H +PT LL T +SEI
Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,813,548
Number of Sequences: 5004
Number of extensions: 59805
Number of successful extensions: 172
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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