Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060661.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g24140.1 68414.m03045 matrixin family protein similar to matr... 53 2e-07
At4g16640.1 68417.m02515 matrix metalloproteinase, putative meta... 46 2e-05
At1g70170.1 68414.m08074 matrixin family protein similar to SP|P... 45 4e-05
At1g59970.1 68414.m06755 matrixin family protein similar to SP|P... 44 7e-05
At2g45040.1 68415.m05607 matrix metalloproteinase nearly identic... 42 3e-04
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 31 0.94
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 31 0.94
At5g46080.1 68418.m05666 protein kinase family protein contains ... 29 2.9
At4g38130.1 68417.m05384 histone deacetylase (RPD3A) identical t... 29 3.8
At4g13750.1 68417.m02134 expressed protein 28 6.6
At2g44470.1 68415.m05529 glycosyl hydrolase family 1 protein con... 28 6.6
At2g17620.1 68415.m02038 cyclin, putative (CYC2a) similar to cyc... 27 8.8
>At1g24140.1 68414.m03045 matrixin family protein similar to matrix
metalloproteinase [Cucumis sativus] GI:7159629; contains
InterPro accession IPR001818: Matrixin
Length = 384
Score = 52.8 bits (121), Expect = 2e-07
Identities = 27/60 (45%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
Frame = +1
Query: 310 ETRSVLARALDVWEQASRLTFTEVNS-EEADILVSFAKRYHDDAYPFDGRGTILAHAFFP 486
E +SV +RA WE+ + LTFT V +DI + F H D PFDG LAHAF P
Sbjct: 188 EVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSP 247
Score = 41.9 bits (94), Expect = 4e-04
Identities = 21/47 (44%), Positives = 27/47 (57%), Gaps = 5/47 (10%)
Frame = +3
Query: 510 HFDDDELWLLEPKDDD-----EEGTSLFAVAVHEFGHSLGLSHSSVK 635
H D +E W++ + D E L +VAVHE GH LGL HSSV+
Sbjct: 253 HLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVE 299
>At4g16640.1 68417.m02515 matrix metalloproteinase, putative
metalloproteinase [Arabidopsis thaliana] GI:3128477;
contains InterPro accession IPR001818: Matrixin
Length = 364
Score = 46.0 bits (104), Expect = 2e-05
Identities = 25/73 (34%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = +1
Query: 271 LSSTRRPSLLDPYETRSVLARALDVWEQASRLTFTEVNS-EEADILVSFAKRYHDDAYPF 447
+S T + L + ++V RA W ++F EV+ AD+ + F H D PF
Sbjct: 168 ISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPF 227
Query: 448 DGRGTILAHAFFP 486
DG LAHAF P
Sbjct: 228 DGVLGTLAHAFAP 240
Score = 39.5 bits (88), Expect = 0.002
Identities = 19/42 (45%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +3
Query: 510 HFDDDELWLLEP--KDDDEEGTSLFAVAVHEFGHSLGLSHSS 629
H D E W+++ K E L +VA HE GH LGL HSS
Sbjct: 246 HLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSS 287
>At1g70170.1 68414.m08074 matrixin family protein similar to
SP|P29136 Metalloendoproteinase 1 precursor (EC
3.4.24.-) (SMEP1) {Glycine max}; contains InterPro
accession IPR001818: Matrixin
Length = 378
Score = 45.2 bits (102), Expect = 4e-05
Identities = 25/60 (41%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +1
Query: 310 ETRSVLARALDVWEQASRLTFTEVNS-EEADILVSFAKRYHDDAYPFDGRGTILAHAFFP 486
E +SV +RA W + L FT S +DI + F H D PFDG LAHAF P
Sbjct: 184 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 243
Score = 39.1 bits (87), Expect = 0.003
Identities = 21/46 (45%), Positives = 25/46 (54%), Gaps = 4/46 (8%)
Frame = +3
Query: 510 HFDDDELWLLEPKDDD----EEGTSLFAVAVHEFGHSLGLSHSSVK 635
H D DE W++ D L +VAVHE GH LGL HSSV+
Sbjct: 249 HLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVE 294
>At1g59970.1 68414.m06755 matrixin family protein similar to
SP|P29136 Metalloendoproteinase 1 precursor (EC
3.4.24.-) (SMEP1) {Glycine max}; contains InterPro
accession IPR001818: Matrixin
Length = 360
Score = 44.4 bits (100), Expect = 7e-05
Identities = 24/60 (40%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +1
Query: 310 ETRSVLARALDVWEQASRLTFTEVNSE-EADILVSFAKRYHDDAYPFDGRGTILAHAFFP 486
E + V +RA W + + L FT S ADI++ F H D PFDG LAHA P
Sbjct: 172 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 231
Score = 37.1 bits (82), Expect = 0.011
Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 6/48 (12%)
Frame = +3
Query: 510 HFDDDELWLLEPKDDDEE------GTSLFAVAVHEFGHSLGLSHSSVK 635
H D DE WL+ + L +VAVHE GH LGL HSSV+
Sbjct: 237 HLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 284
>At2g45040.1 68415.m05607 matrix metalloproteinase nearly identical
to metalloproteinase [Arabidopsis thaliana] GI:3128477;
contains InterPro accession IPR001818: Matrixin
Length = 342
Score = 42.3 bits (95), Expect = 3e-04
Identities = 24/64 (37%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
Frame = +1
Query: 298 LDPYETRSVLARALDVWEQASRLTFTEVNSEE-ADILVSFAKRYHDDAYPFDGRGTILAH 474
L P + R V RA W ++F E ADI + F H D PFDG +LAH
Sbjct: 155 LAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAH 214
Query: 475 AFFP 486
F P
Sbjct: 215 TFSP 218
Score = 41.9 bits (94), Expect = 4e-04
Identities = 20/43 (46%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = +3
Query: 510 HFDDDELWLLE-PKDDDEEGTSLFAVAVHEFGHSLGLSHSSVK 635
H D E W ++ ++ L +VAVHE GH LGL HSSVK
Sbjct: 224 HLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVK 266
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 30.7 bits (66), Expect = 0.94
Identities = 19/48 (39%), Positives = 27/48 (56%)
Frame = +3
Query: 18 SPEEEERANRRQEKTRSYALRRSSHEDRVNAILKPSLFSSNQSQHPFR 161
S E+E A +R E T++ +R + E R NAIL+ SL Q+ H R
Sbjct: 567 SSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHERR 614
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 30.7 bits (66), Expect = 0.94
Identities = 19/48 (39%), Positives = 27/48 (56%)
Frame = +3
Query: 18 SPEEEERANRRQEKTRSYALRRSSHEDRVNAILKPSLFSSNQSQHPFR 161
S E+E A +R E T++ +R + E R NAIL+ SL Q+ H R
Sbjct: 567 SSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHERR 614
>At5g46080.1 68418.m05666 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 332
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/21 (52%), Positives = 17/21 (80%)
Frame = -2
Query: 78 AGRRSESFLVVDWLSPLLLER 16
+GRRSE+ L+V W +PL+ E+
Sbjct: 256 SGRRSENGLIVKWATPLIKEQ 276
>At4g38130.1 68417.m05384 histone deacetylase (RPD3A) identical to
SP|O22446 Histone deacetylase (HD) {Arabidopsis
thaliana}
Length = 501
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +2
Query: 65 LLRPAPVKPRGPCERHTEAFVIFLEPIPAPVPRQPLRQ 178
+L+P P + R C H + +V FL I + +RQ
Sbjct: 61 VLKPFPARDRDLCRFHADDYVSFLRSITPETQQDQIRQ 98
>At4g13750.1 68417.m02134 expressed protein
Length = 2137
Score = 27.9 bits (59), Expect = 6.6
Identities = 22/81 (27%), Positives = 38/81 (46%), Gaps = 3/81 (3%)
Frame = +3
Query: 447 RWTRYDSSSRFLSW-RGPRW*RHFDD-DELWLLEPKDDDEEGTSLFAVAVHEFGHSLGL- 617
+W SS + W + + F + D++ + ++D+EG + V HSLG+
Sbjct: 1650 KWVSLHSSFGLVCWCDNEKLKKRFKNKDKIEFISFGENDDEGQEVLQTKVSGLMHSLGIP 1709
Query: 618 SHSSVKRRTDVXFGYQGIQSN 680
S S V +R Y+G+Q N
Sbjct: 1710 SISEVVKR---EAKYEGLQDN 1727
>At2g44470.1 68415.m05529 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to anther-specific protein ATA27 (GI:2746341)
[Arabidopsis thaliana]
Length = 451
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/32 (43%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = +1
Query: 343 VWEQASRLTFTE-VNSEEADILVSFAKRYHDD 435
+W+ S TF E N + AD+ V F RY DD
Sbjct: 62 IWDYFSH-TFPERTNMQNADVAVDFYHRYKDD 92
>At2g17620.1 68415.m02038 cyclin, putative (CYC2a) similar to cyclin
2b protein [Arabidopsis thaliana] GI:509423; contains
Pfam profiles PF00134: Cyclin, N-terminal domain,
PF02984: Cyclin, C-terminal domain; identical to cDNA
cyc2a mRNA for cyclin 2a protein GI:728518
Length = 429
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +3
Query: 24 EEEERANRRQEKTRSYALRRSSHEDRVNAILKPSLFSSN 140
EEEE +++ + ++ RS E+ N LKPS+ S+N
Sbjct: 69 EEEEGCQKKKFDSLRPSVTRSGVEEETNKKLKPSVPSAN 107
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,809,259
Number of Sequences: 28952
Number of extensions: 358360
Number of successful extensions: 1071
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1035
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1070
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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