BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060660.seq (681 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 159 3e-40 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 152 5e-38 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 138 7e-34 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 136 2e-33 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 102 4e-23 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 56 5e-09 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 44 2e-05 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 32 0.088 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.82 SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 1.9 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 27 3.3 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 7.7 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 7.7 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 159 bits (387), Expect = 3e-40 Identities = 86/146 (58%), Positives = 99/146 (67%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423 P IFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603 VL KM+ETAEAYLG V +AV+TVPAYF QRQATKDA I+GL NE Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGL-NVLRIINEP-TAAA 178 Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681 AYG D+ NVL + GGT Sbjct: 179 IAYGLDRSNQ-GESNVL-IFDLGGGT 202 Score = 115 bits (276), Expect = 7e-27 Identities = 52/60 (86%), Positives = 57/60 (95%) Frame = +2 Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 152 bits (368), Expect = 5e-38 Identities = 82/146 (56%), Positives = 98/146 (67%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423 P IFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603 VL KM+E+AEA+LG V +AV+TVPAYF QRQATKDA I+GL NE Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGL-NVLRIINEP-TAAA 178 Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681 AYG D+ + NVL + GGT Sbjct: 179 IAYGLDRSNQHET-NVL-IFDLGGGT 202 Score = 115 bits (276), Expect = 7e-27 Identities = 52/60 (86%), Positives = 57/60 (95%) Frame = +2 Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 138 bits (334), Expect = 7e-34 Identities = 77/146 (52%), Positives = 93/146 (63%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423 P+ +FDAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+M Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603 VLTKMKE +EA L K V+ AVITVPAYF QR ATKDA I+GL NE Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGL-NVLRIINEP-TAAA 182 Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681 AYG D K K NVL + GGT Sbjct: 183 IAYGLDAKSD-KPKNVL-IFDLGGGT 206 Score = 102 bits (245), Expect = 4e-23 Identities = 46/60 (76%), Positives = 52/60 (86%) Frame = +2 Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 136 bits (330), Expect = 2e-33 Identities = 69/127 (54%), Positives = 89/127 (70%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423 P+ IFD KRLIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F PEE+S+M Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603 +L+KMK+TAEAYLGK V +AV+TVPAYF QRQATKDA TI+GL NE Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGL-NVIRIVNEP-TAAA 211 Query: 604 FAYGXDK 624 AYG DK Sbjct: 212 IAYGLDK 218 Score = 100 bits (240), Expect = 2e-22 Identities = 44/59 (74%), Positives = 51/59 (86%) Frame = +2 Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP N Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPEN 96 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 102 bits (245), Expect = 4e-23 Identities = 60/146 (41%), Positives = 81/146 (55%) Frame = +1 Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423 P+ F KRLIGR+F++ VQ D+K P+++V + KT+ P ++ Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166 Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603 +L+KM+ETA YLGK V+NAV+TVPAYF QRQATK A I+GL NE Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGL-NVLRVVNEP-TAAA 224 Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681 AYG DK KN ++ + GGT Sbjct: 225 LAYGLDK----KNDAIVAVFDLGGGT 246 Score = 76.2 bits (179), Expect = 4e-15 Identities = 35/66 (53%), Positives = 46/66 (69%), Gaps = 1/66 (1%) Frame = +2 Query: 65 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 241 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 242 NPNNKY 259 NP N + Sbjct: 109 NPENTF 114 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 56.0 bits (129), Expect = 5e-09 Identities = 36/120 (30%), Positives = 59/120 (49%), Gaps = 1/120 (0%) Frame = +1 Query: 268 KRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444 KRL GR ++D ++ + ++ G KV Y E+ F ++ + TK+K Sbjct: 72 KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131 Query: 445 TAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCRFAYGXDK 624 AEA L +V + VI++PA+F +QR+A +A I+GL +P + YG K Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQRRALLEAANIAGL--NPLRLMNDNAAAALTYGITK 189 Score = 52.0 bits (119), Expect = 8e-08 Identities = 22/64 (34%), Positives = 40/64 (62%) Frame = +2 Query: 62 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 241 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 242 NPNN 253 N N Sbjct: 63 NFRN 66 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 44.0 bits (99), Expect = 2e-05 Identities = 18/59 (30%), Positives = 33/59 (55%) Frame = +2 Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N +N Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADN 85 Score = 42.3 bits (95), Expect = 6e-05 Identities = 21/58 (36%), Positives = 32/58 (55%) Frame = +1 Query: 385 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGL 558 ++K E S L ++ E+AE +LG V V++VP YF QR+A + A +GL Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGL 191 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 31.9 bits (69), Expect = 0.088 Identities = 23/104 (22%), Positives = 45/104 (43%), Gaps = 2/104 (1%) Frame = +1 Query: 244 PQQQIFDAKRLIGRK-FEDATVQADMKHWPFEVVSDGGKPKIKVAYK-GEDKTFFPEEVS 417 P I + K L+ E VQ +P + + + +++ +++ + EE+ Sbjct: 82 PAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEII 141 Query: 418 SMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTI 549 +M + AE + + + V+TVP +F LQR +A I Sbjct: 142 AMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185 Score = 29.9 bits (64), Expect = 0.36 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Frame = +2 Query: 77 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253 + ID GT ++ + + G +EI+ R S VAF ER+ G A N P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82 Query: 254 KYSMPNV 274 +S+ NV Sbjct: 83 AHSIRNV 89 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.82 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 69 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 164 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 2244 Score = 27.5 bits (58), Expect = 1.9 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = +2 Query: 173 PSYVAFTDTERLIGDAAKNQVAMNPNNKYSMPNVSSDVSSKMLLC 307 P +A T ER++ AAK A+N Y++ ++ D K++ C Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 26.6 bits (56), Expect = 3.3 Identities = 20/95 (21%), Positives = 44/95 (46%), Gaps = 11/95 (11%) Frame = -2 Query: 488 ITAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPL-----------YATLILGLPPSL 342 I++ T+L + S + +S+ + S +LSS + Y++ ++ +L Sbjct: 618 ISSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSISTIPISSSLSTYSSSVIPSSSTL 677 Query: 341 TTSKGQCFMSACTVASSNLRPMRRLASNICCWGSS 237 +S +S+ VASS+ P+ +S + + +S Sbjct: 678 VSSSSSLIVSSSPVASSSSSPIPSSSSLVSTYSAS 712 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.4 bits (53), Expect = 7.7 Identities = 17/66 (25%), Positives = 31/66 (46%) Frame = +1 Query: 55 QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 234 +NGK T +R +S + W P++E R G D + V R + + +EP Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531 Query: 235 GDEPQQ 252 ++P++ Sbjct: 532 -EKPEE 536 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.4 bits (53), Expect = 7.7 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = -2 Query: 155 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 51 G W SP S + H ++++ +F +RVL C+ Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,908,112 Number of Sequences: 5004 Number of extensions: 62166 Number of successful extensions: 221 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 200 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 219 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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