SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060660.seq
         (681 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   159   3e-40
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   152   5e-38
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...   138   7e-34
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   136   2e-33
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...   102   4e-23
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    56   5e-09
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    44   2e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    32   0.088
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar...    29   0.82 
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo...    27   1.9  
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    27   3.3  
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar...    25   7.7  
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar...    25   7.7  

>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  159 bits (387), Expect = 3e-40
 Identities = 86/146 (58%), Positives = 99/146 (67%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P   IFDAKRLIGRKF+D  VQ+DMKHWPF+V+S  GKP ++V YKGE KTF PEE+SSM
Sbjct: 61  PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
           VL KM+ETAEAYLG  V +AV+TVPAYF   QRQATKDA  I+GL       NE      
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGL-NVLRIINEP-TAAA 178

Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681
            AYG D+       NVL +    GGT
Sbjct: 179 IAYGLDRSNQ-GESNVL-IFDLGGGT 202



 Score =  115 bits (276), Expect = 7e-27
 Identities = 52/60 (86%), Positives = 57/60 (95%)
 Frame = +2

Query: 74  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  152 bits (368), Expect = 5e-38
 Identities = 82/146 (56%), Positives = 98/146 (67%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P   IFDAKRLIGR+F D  VQ+DMKHWPF+V+   GKP I+V +KGE KTF PEE+SSM
Sbjct: 61  PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
           VL KM+E+AEA+LG  V +AV+TVPAYF   QRQATKDA  I+GL       NE      
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGL-NVLRIINEP-TAAA 178

Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681
            AYG D+    +  NVL +    GGT
Sbjct: 179 IAYGLDRSNQHET-NVL-IFDLGGGT 202



 Score =  115 bits (276), Expect = 7e-27
 Identities = 52/60 (86%), Positives = 57/60 (95%)
 Frame = +2

Query: 74  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score =  138 bits (334), Expect = 7e-34
 Identities = 77/146 (52%), Positives = 93/146 (63%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P+  +FDAKRLIGR++ED   Q D+KHWPF+V+ + G P I+V Y GE K F  +E+S+M
Sbjct: 65  PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
           VLTKMKE +EA L K V+ AVITVPAYF   QR ATKDA  I+GL       NE      
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGL-NVLRIINEP-TAAA 182

Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681
            AYG D K   K  NVL +    GGT
Sbjct: 183 IAYGLDAKSD-KPKNVL-IFDLGGGT 206



 Score =  102 bits (245), Expect = 4e-23
 Identities = 46/60 (76%), Positives = 52/60 (86%)
 Frame = +2

Query: 74  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           A+GIDLGTTYSCV V++   VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8   AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  136 bits (330), Expect = 2e-33
 Identities = 69/127 (54%), Positives = 89/127 (70%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P+  IFD KRLIGRKF++ T+  D+K +PF +V+D  +P ++V   G+ K F PEE+S+M
Sbjct: 94  PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
           +L+KMK+TAEAYLGK V +AV+TVPAYF   QRQATKDA TI+GL       NE      
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGL-NVIRIVNEP-TAAA 211

Query: 604 FAYGXDK 624
            AYG DK
Sbjct: 212 IAYGLDK 218



 Score =  100 bits (240), Expect = 2e-22
 Identities = 44/59 (74%), Positives = 51/59 (86%)
 Frame = +2

Query: 77  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ   NP N
Sbjct: 38  IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPEN 96


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score =  102 bits (245), Expect = 4e-23
 Identities = 60/146 (41%), Positives = 81/146 (55%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
           P+   F  KRLIGR+F++  VQ D+K  P+++V            +   KT+ P ++   
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166

Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
           +L+KM+ETA  YLGK V+NAV+TVPAYF   QRQATK A  I+GL       NE      
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGL-NVLRVVNEP-TAAA 224

Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681
            AYG DK    KN  ++ +    GGT
Sbjct: 225 LAYGLDK----KNDAIVAVFDLGGGT 246



 Score = 76.2 bits (179), Expect = 4e-15
 Identities = 35/66 (53%), Positives = 46/66 (69%), Gaps = 1/66 (1%)
 Frame = +2

Query: 65  KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 241
           K P +GIDLGTT SC+ + +    ++IAN +G RTTPS VAFT D ERL+G +AK Q  +
Sbjct: 49  KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108

Query: 242 NPNNKY 259
           NP N +
Sbjct: 109 NPENTF 114


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 56.0 bits (129), Expect = 5e-09
 Identities = 36/120 (30%), Positives = 59/120 (49%), Gaps = 1/120 (0%)
 Frame = +1

Query: 268 KRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444
           KRL GR ++D  ++  +      ++    G    KV Y  E+  F   ++ +   TK+K 
Sbjct: 72  KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131

Query: 445 TAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCRFAYGXDK 624
            AEA L  +V + VI++PA+F  +QR+A  +A  I+GL  +P      +      YG  K
Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQRRALLEAANIAGL--NPLRLMNDNAAAALTYGITK 189



 Score = 52.0 bits (119), Expect = 8e-08
 Identities = 22/64 (34%), Positives = 40/64 (62%)
 Frame = +2

Query: 62  AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 241
           ++   VGID G + + + V ++  +++I N+  NR+TPS V++ +  R +G+AAK+  A 
Sbjct: 3   SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62

Query: 242 NPNN 253
           N  N
Sbjct: 63  NFRN 66


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 18/59 (30%), Positives = 33/59 (55%)
 Frame = +2

Query: 77  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           +GI  G   S +   + GK +++AN++GNR  PS +++   +   G  A+ Q+  N +N
Sbjct: 27  IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADN 85



 Score = 42.3 bits (95), Expect = 6e-05
 Identities = 21/58 (36%), Positives = 32/58 (55%)
 Frame = +1

Query: 385 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGL 558
           ++K     E S   L ++ E+AE +LG  V   V++VP YF   QR+A + A   +GL
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGL 191


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 31.9 bits (69), Expect = 0.088
 Identities = 23/104 (22%), Positives = 45/104 (43%), Gaps = 2/104 (1%)
 Frame = +1

Query: 244 PQQQIFDAKRLIGRK-FEDATVQADMKHWPFEVVSDGGKPKIKVAYK-GEDKTFFPEEVS 417
           P   I + K L+     E   VQ     +P   + +  +    +++   +++ +  EE+ 
Sbjct: 82  PAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEII 141

Query: 418 SMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTI 549
           +M +      AE    + + + V+TVP +F  LQR    +A  I
Sbjct: 142 AMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185



 Score = 29.9 bits (64), Expect = 0.36
 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
 Frame = +2

Query: 77  VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 253
           + ID GT ++   + + G  +EI+      R   S VAF   ER+ G  A N     P  
Sbjct: 25  LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82

Query: 254 KYSMPNV 274
            +S+ NV
Sbjct: 83  AHSIRNV 89


>SPAC25B8.04c |||mitochondrial splicing suppressor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 378

 Score = 28.7 bits (61), Expect = 0.82
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +3

Query: 69  HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 164
           HP   S W+P    LVS+    W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314


>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2244

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 14/45 (31%), Positives = 24/45 (53%)
 Frame = +2

Query: 173  PSYVAFTDTERLIGDAAKNQVAMNPNNKYSMPNVSSDVSSKMLLC 307
            P  +A T  ER++  AAK   A+N    Y++  ++ D   K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 20/95 (21%), Positives = 44/95 (46%), Gaps = 11/95 (11%)
 Frame = -2

Query: 488 ITAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPL-----------YATLILGLPPSL 342
           I++  T+L     + S + +S+  + S    +LSS +           Y++ ++    +L
Sbjct: 618 ISSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSISTIPISSSLSTYSSSVIPSSSTL 677

Query: 341 TTSKGQCFMSACTVASSNLRPMRRLASNICCWGSS 237
            +S     +S+  VASS+  P+   +S +  + +S
Sbjct: 678 VSSSSSLIVSSSPVASSSSSPIPSSSSLVSTYSAS 712


>SPAC2F7.03c |pom1||DYRK family protein kinase
           Pom1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1087

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 17/66 (25%), Positives = 31/66 (46%)
 Frame = +1

Query: 55  QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 234
           +NGK T +R +S     + W  P++E    R   G  D +    V R   +  +  +EP 
Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531

Query: 235 GDEPQQ 252
            ++P++
Sbjct: 532 -EKPEE 536


>SPAC823.11 |||sphingosine-1-phosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 411

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 11/35 (31%), Positives = 19/35 (54%)
 Frame = -2

Query: 155 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 51
           G W  SP S +  H   ++++  +F +RVL   C+
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,908,112
Number of Sequences: 5004
Number of extensions: 62166
Number of successful extensions: 221
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -