Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060660.seq
(681 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 165 3e-41
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 163 1e-40
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 142 2e-34
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 132 2e-31
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 80 1e-15
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 76 2e-14
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 69 3e-12
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 44 1e-04
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 6e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.86
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.6
SB_50110| Best HMM Match : Ank (HMM E-Value=4.6e-20) 29 4.6
SB_48511| Best HMM Match : Ank (HMM E-Value=4.6e-20) 29 4.6
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 4.6
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.1
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 165 bits (401), Expect = 3e-41
Identities = 85/146 (58%), Positives = 102/146 (69%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
VLTKMKETAEAYLG V +AV+TVPAYF QRQATKDA TI+GL NE
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGL-NVLRIINEP-TAAA 180
Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681
AYG DKK ++ ++Y GGT
Sbjct: 181 IAYGLDKKVGVERNVLIY--DLGGGT 204
Score = 134 bits (324), Expect = 6e-32
Identities = 62/65 (95%), Positives = 63/65 (96%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 239 MNPNN 253
MNP N
Sbjct: 61 MNPEN 65
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 163 bits (396), Expect = 1e-40
Identities = 87/146 (59%), Positives = 102/146 (69%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ IFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
VL KMKETAEAYLG+ V +AVITVPAYF QRQATKDA TI+GL NE
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGL-NVLRVINEP-TAAA 180
Query: 604 FAYGXDKKGYLKNGNVLYLXTSAGGT 681
AYG DK L + + GGT
Sbjct: 181 LAYGLDKS--LSGEKNVLIFDLGGGT 204
Score = 129 bits (312), Expect = 2e-30
Identities = 58/65 (89%), Positives = 62/65 (95%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 239 MNPNN 253
+NP N
Sbjct: 61 LNPEN 65
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 142 bits (345), Expect = 2e-34
Identities = 76/133 (57%), Positives = 91/133 (68%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCR 603
VLTKMKETAEAYLGK V +AV+TVPAYF QRQATKDA I+GL NE
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGL-TVMRIINEP-TAAA 878
Query: 604 FAYGXDKKGYLKN 642
AYG DKK KN
Sbjct: 879 IAYGMDKKEGEKN 891
Score = 102 bits (244), Expect = 3e-22
Identities = 47/60 (78%), Positives = 53/60 (88%), Gaps = 1/60 (1%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 253
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 132 bits (320), Expect = 2e-31
Identities = 60/65 (92%), Positives = 62/65 (95%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 239 MNPNN 253
MNP N
Sbjct: 61 MNPTN 65
Score = 128 bits (309), Expect = 4e-30
Identities = 72/124 (58%), Positives = 81/124 (65%), Gaps = 25/124 (20%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV--- 414
P IFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 415 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQA 528
SSMVL KMKETAEAYLG V +AV+TVPAYF QRQA
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQA 182
Query: 529 TKDA 540
TKDA
Sbjct: 183 TKDA 186
Score = 79.0 bits (186), Expect = 3e-15
Identities = 51/91 (56%), Positives = 57/91 (62%)
Frame = +1
Query: 409 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNET 588
++SSMVL KMKETAEAYLG V +AV+TVPAYF QRQATKDA ISGL NE
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGL-NVLRIINEP 269
Query: 589 HCCCRFAYGXDKKGYLKNGNVLYLXTSAGGT 681
AYG DKK + NVL + GGT
Sbjct: 270 -TAAAIAYGLDKK-VGQERNVL-IFDLGGGT 297
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 80.2 bits (189), Expect = 1e-15
Identities = 47/121 (38%), Positives = 67/121 (55%), Gaps = 1/121 (0%)
Frame = +1
Query: 268 KRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444
KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M+ T++K
Sbjct: 127 KRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKT 186
Query: 445 TAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCCRFAYGXDK 624
TAE L V + VI+VP+Y+ QR+ DA +GL N+T AYG K
Sbjct: 187 TAEIALKTKVTDCVISVPSYYTDRQRRCMLDASATAGL-NCLRLMNDT-TAVSLAYGIYK 244
Query: 625 K 627
+
Sbjct: 245 Q 245
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 232
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 76.2 bits (179), Expect = 2e-14
Identities = 37/65 (56%), Positives = 50/65 (76%)
Frame = +1
Query: 364 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAV 543
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+F QRQATKDA
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61
Query: 544 TISGL 558
TI+GL
Sbjct: 62 TIAGL 66
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 69.3 bits (162), Expect = 3e-12
Identities = 52/147 (35%), Positives = 70/147 (47%), Gaps = 1/147 (0%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEVSS 420
P+ I+DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 421 MVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKDAVTISGLKRSPNSFNETHCCC 600
V+ K++ AE L TV AV++VPA F QR AT A +++G+ NE
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGI-TVLRVINEP-TAA 209
Query: 601 RFAYGXDKKGYLKNGNVLYLXTSAGGT 681
AYG KK + N V+ L GGT
Sbjct: 210 ALAYGLHKKEGVANVMVVDL---GGGT 233
Score = 67.7 bits (158), Expect = 8e-12
Identities = 30/65 (46%), Positives = 44/65 (67%), Gaps = 2/65 (3%)
Frame = +2
Query: 65 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 239 MNPNN 253
+NP N
Sbjct: 90 INPKN 94
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 44.0 bits (99), Expect = 1e-04
Identities = 22/67 (32%), Positives = 36/67 (53%)
Frame = +1
Query: 358 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLQRQATKD 537
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +F +R+A
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126
Query: 538 AVTISGL 558
A + GL
Sbjct: 127 AAELVGL 133
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2
Query: 128 GKVEIIANDQGNRTTPSYVAFTDTERLI 211
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.86
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +2
Query: 137 EIIANDQGNRTTPSYVAFT-DTERL 208
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -2
Query: 245 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 147
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_50110| Best HMM Match : Ank (HMM E-Value=4.6e-20)
Length = 296
Score = 28.7 bits (61), Expect = 4.6
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +3
Query: 429 YENEGNCR-SLSRQNCAECSYHGSRVLHDSSKTSHKRCSNHLRLETFSE 572
Y+N CR SLS + Y S +LHD+S+T+ + S L E E
Sbjct: 148 YKNLPGCRYSLSSGSTDSGVYDDSNLLHDNSETTRQPRSQRLARENSYE 196
>SB_48511| Best HMM Match : Ank (HMM E-Value=4.6e-20)
Length = 753
Score = 28.7 bits (61), Expect = 4.6
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +3
Query: 429 YENEGNCR-SLSRQNCAECSYHGSRVLHDSSKTSHKRCSNHLRLETFSE 572
Y+N CR SLS + Y S +LHD+S+T+ + S L E E
Sbjct: 148 YKNLPGCRYSLSSGSTDSGVYDDSNLLHDNSETTRQPRSQRLARENSYE 196
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 4.6
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 5 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 91
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.3 bits (60), Expect = 6.1
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -2
Query: 452 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRP 279
S F+F ST + +S +KV+ SPL + + P+ G S C V + + P
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCP 1165
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,575,163
Number of Sequences: 59808
Number of extensions: 522846
Number of successful extensions: 1486
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1364
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1479
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1757375282
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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