BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060654.seq (687 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 73 3e-14 SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide 1,2-al... 33 0.029 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 33 0.029 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 26 4.4 SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomy... 26 4.4 SPAC10F6.04 |||RCC domain protein Ats1 |Schizosaccharomyces pomb... 26 5.9 SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 26 5.9 >SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 787 Score = 73.3 bits (172), Expect = 3e-14 Identities = 45/127 (35%), Positives = 66/127 (51%), Gaps = 4/127 (3%) Frame = +1 Query: 274 MSKAERLSLRDESRQMFYHAYHAYMENAYPADELMPLSCKGRWXGI-TPSRGDMDDALGN 450 ++ A + LR+ SR++FYH Y+ YM+ A+P DEL PLSC+G P+ ++D G+ Sbjct: 27 INSARMVELRETSRRLFYHGYNNYMQFAFPNDELAPLSCEGLGPDYENPNNIGVNDVRGD 86 Query: 451 FXXXXXXXXXXXXXMGD---FSDLIMQSNSL*KMSLLTKILVVSVFETNIRMLGGLLSAH 621 + +GD F D + + TK V VFE IR+LGGLLS+H Sbjct: 87 YLLTLVDVLDTLVVLGDREGFQDAVDKVIHHINFERDTK---VQVFEATIRILGGLLSSH 143 Query: 622 VLAETLK 642 + A K Sbjct: 144 IFASEEK 150 >SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide 1,2-alpha-mannosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 33.5 bits (73), Expect = 0.029 Identities = 15/22 (68%), Positives = 20/22 (90%) Frame = +1 Query: 568 VSVFETNIRMLGGLLSAHVLAE 633 VSVFET IR+LGGLLS++ L++ Sbjct: 126 VSVFETTIRILGGLLSSYHLSQ 147 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 33.5 bits (73), Expect = 0.029 Identities = 16/43 (37%), Positives = 24/43 (55%) Frame = -2 Query: 557 LVKRDIFYNELDCMIKSEKSPITTKVSKLSTNVNEKFPKASSI 429 L+++ Y E + SE ++ KVS L+ VNE PKAS + Sbjct: 1208 LLRQSASYKEKLSLASSENKDLSNKVSSLTKQVNELSPKASKV 1250 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 26.2 bits (55), Expect = 4.4 Identities = 9/22 (40%), Positives = 12/22 (54%) Frame = -3 Query: 370 HQLDMHSPCKHDKHDRTSALIH 305 H D H C H + DR S L++ Sbjct: 325 HYFDFHKECSHMRWDRVSLLLN 346 >SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 735 Score = 26.2 bits (55), Expect = 4.4 Identities = 10/18 (55%), Positives = 14/18 (77%) Frame = -1 Query: 600 QHPNICFKD*NNQYLGQK 547 Q N+CFKD + +Y+GQK Sbjct: 423 QLQNLCFKDNDIKYIGQK 440 >SPAC10F6.04 |||RCC domain protein Ats1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 351 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/25 (44%), Positives = 17/25 (68%), Gaps = 3/25 (12%) Frame = +3 Query: 444 RKFLIDIGGQLRHFSC---DGGLFG 509 ++F+IDI LRH+ C +G L+G Sbjct: 144 KEFIIDISAGLRHWICVTNEGNLYG 168 >SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/42 (28%), Positives = 19/42 (45%) Frame = -3 Query: 565 PISWSKETSFITSWIA*LNPKSPPSQLKCLSCPPMSMRNFLK 440 P +W F+T + + +P + K L C P M N L+ Sbjct: 236 PENWKGSVGFVTQELIKAHFPAPSPETKVLICGPTPMVNSLR 277 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,901,105 Number of Sequences: 5004 Number of extensions: 60833 Number of successful extensions: 131 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 130 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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