Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060654.seq
         (687 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein s...    79   3e-15
At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein S...    51   6e-07
At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein s...    46   2e-05
At1g51590.1 68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mann...    31   0.54 
At3g21160.1 68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mann...    30   1.3  
At1g29790.1 68414.m03642 expressed protein                             29   2.9  

>At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein
           similar to mannosyl-oligosaccharide
           1,2-alpha-mannosidase IB [Mus musculus][SP|P39098]
          Length = 624

 Score = 79.0 bits (186), Expect = 3e-15
 Identities = 49/133 (36%), Positives = 64/133 (48%)
 Frame = +1

Query: 286 ERLSLRDESRQMFYHAYHAYMENAYPADELMPLSCKGRWXGITPSRGDMDDALGNFXXXX 465
           E   LRDE R MFYHA+  YM NA+P DEL PLSC+G            +D LG +    
Sbjct: 36  EAKQLRDEVRGMFYHAFDGYMNNAFPLDELRPLSCQG------------EDTLGGYALTL 83

Query: 466 XXXXXXXXXMGDFSDLIMQSNSL*KMSLLTKILVVSVFETNIRMLGGLLSAHVLAETLKS 645
                    +GD          + K         VSVFET IR+LGGLLSAH++A    +
Sbjct: 84  IDSLDTLALLGDRERFTSSVEWIGKNLQFNINKTVSVFETTIRVLGGLLSAHLIASDYAT 143

Query: 646 DIPLXQWYNXGIV 684
            + +  + N  +V
Sbjct: 144 GMRIPSYNNELLV 156


>At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein
           Similar to gb|U04299 mannosyl-oligosaccharide
           alpha-1,2-mannosidase from Mus musculus. ESTs gb|R84145
           and gb|AA394707 come from this gene
          Length = 574

 Score = 51.2 bits (117), Expect = 6e-07
 Identities = 46/129 (35%), Positives = 63/129 (48%), Gaps = 12/129 (9%)
 Frame = +1

Query: 280 KAERLSLRDESRQMFYHAYHAYMENAYPADELMPLSCKGRWXGITPSRGDMDDALGNF-- 453
           + ++  +R++ R+MFYHAY  YM  A+P DEL PL         T S  D    LGN   
Sbjct: 31  EVKKKQMREKVREMFYHAYDNYMTYAFPHDELKPL---------TKSFTDSLSELGNLKL 81

Query: 454 -XXXXXXXXXXXXXMGDFSDLIMQSNS--L*KMSL-LTKILV------VSVFETNIRMLG 603
                         +   S L +  NS    K  L L++ L       V++FE NIR+LG
Sbjct: 82  EHLPTDYNGSAVTLVESLSSLAILGNSTEFEKGVLWLSENLTFDIDARVNLFECNIRVLG 141

Query: 604 GLLSAHVLA 630
           GL+SAH+LA
Sbjct: 142 GLISAHLLA 150


>At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein
           similar to GI:5579331 from [Homo sapiens]; contains Pfam
           profile PF01532: Glycosyl hydrolase family 47
          Length = 624

 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 41/129 (31%), Positives = 65/129 (50%), Gaps = 5/129 (3%)
 Frame = +1

Query: 259 TSGLRMSKAE-RLSLRDES-RQMFYHAYHAYMENAYPADELMPLSCKGRWXGITPSRGDM 432
           T+G  +S ++ + + R +S ++ F HA+  Y + A   DELMP+S KG            
Sbjct: 113 TNGSTISNSDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGV----------- 161

Query: 433 DDALGNFXXXXXXXXXXXXXMGDFSDLIMQSNSL*KMSLLTKILV---VSVFETNIRMLG 603
            D LG               MG   +++ ++ S  +  LL +I     V++FET IR+LG
Sbjct: 162 -DGLGGLGATVVDALDTAMIMG-LDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLG 219

Query: 604 GLLSAHVLA 630
           GLLSA+ L+
Sbjct: 220 GLLSAYHLS 228


>At1g51590.1 68414.m05808 mannosyl-oligosaccharide
           1,2-alpha-mannosidase, putative similar to
           mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
           max][GI:6552504]
          Length = 560

 Score = 31.5 bits (68), Expect = 0.54
 Identities = 30/111 (27%), Positives = 47/111 (42%), Gaps = 1/111 (0%)
 Frame = +1

Query: 301 RDESRQMFYHAYHAYMENAYPADELMPLSCKGRWXGITPSRGDMDDALGNFXXXXXXXXX 480
           R + ++   HA+ +Y + A+  DEL P +  G             D+ G           
Sbjct: 98  RQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGT------------DSFGGLGATMVDSLD 145

Query: 481 XXXXMGDFSDLIMQSNSL*KMSL-LTKILVVSVFETNIRMLGGLLSAHVLA 630
               MG   +   ++      SL   K    S+FET IR++GGLLSA+ L+
Sbjct: 146 TLYIMG-LDEQFQKAREWVASSLDFDKDYDASMFETTIRVVGGLLSAYDLS 195


>At3g21160.1 68416.m02673 mannosyl-oligosaccharide
           1,2-alpha-mannosidase, putative similar to
           mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
           max][GI:6552504]
          Length = 572

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 14/25 (56%), Positives = 19/25 (76%)
 Frame = +1

Query: 556 KILVVSVFETNIRMLGGLLSAHVLA 630
           K    S+FET IR++GGLLSA+ L+
Sbjct: 172 KDYAASMFETTIRVVGGLLSAYDLS 196


>At1g29790.1 68414.m03642 expressed protein
          Length = 378

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 13/34 (38%), Positives = 23/34 (67%)
 Frame = -2

Query: 482 VSKLSTNVNEKFPKASSISPRLGVIPXHLPLQLR 381
           V+ L+T +N   P + +++ R G++P H+PLQ R
Sbjct: 248 VTVLTTTMNFNAPYSEAVAMR-GLVPLHVPLQQR 280


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,915,507
Number of Sequences: 28952
Number of extensions: 315924
Number of successful extensions: 674
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 672
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)