Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060648.seq
(436 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g21280.1 68416.m02689 ubiquitin-specific protease 7, putative... 64 3e-11
At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative... 60 6e-10
At5g49570.1 68418.m06135 transglutaminase-like family protein lo... 33 0.11
At5g42220.1 68418.m05139 ubiquitin family protein contains INTER... 32 0.15
At4g30890.2 68417.m04387 ubiquitin-specific protease 24, putativ... 32 0.15
At4g30890.1 68417.m04386 ubiquitin-specific protease 24, putativ... 32 0.15
At1g11980.1 68414.m01384 ubiquitin family protein similar to Cha... 31 0.25
At4g06599.1 68417.m01024 ubiquitin family protein contains Pfam ... 31 0.34
At2g40930.1 68415.m05052 ubiquitin-specific protease 5, putative... 31 0.34
At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15) ... 31 0.34
At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase f... 31 0.44
At3g14400.1 68416.m01822 ubiquitin-specific protease 25 (UBP25) ... 30 0.59
At1g32850.1 68414.m04048 ubiquitin carboxyl-terminal hydrolase f... 30 0.59
At5g22030.2 68418.m02564 ubiquitin-specific protease 8, putative... 29 1.8
At5g22030.1 68418.m02563 ubiquitin-specific protease 8, putative... 29 1.8
At4g10590.2 68417.m01733 ubiquitin carboxyl-terminal hydrolase f... 29 1.8
At4g10590.1 68417.m01732 ubiquitin carboxyl-terminal hydrolase f... 29 1.8
At4g10570.1 68417.m01730 ubiquitin carboxyl-terminal hydrolase f... 29 1.8
At2g32780.1 68415.m04013 ubiquitin-specific protease 1, putative... 29 1.8
At1g04860.1 68414.m00482 ubiquitin-specific protease 2 (UBP2) id... 28 2.4
At4g24560.1 68417.m03520 ubiquitin-specific protease 16, putativ... 27 4.1
At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putativ... 27 5.5
At4g20700.1 68417.m03008 hypothetical protein 27 5.5
At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26) ... 27 5.5
At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putativ... 27 5.5
At5g25010.1 68418.m02964 expressed protein ; expression supporte... 27 7.2
At2g12550.1 68415.m01357 ubiquitin-associated (UBA)/TS-N domain-... 27 7.2
At5g10790.1 68418.m01254 ubiquitin-specific protease 22 (UBP22) ... 26 9.5
At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12) ... 26 9.5
At5g06600.1 68418.m00745 ubiquitin-specific protease 12 (UBP12) ... 26 9.5
At3g11910.1 68416.m01460 ubiquitin-specific protease, putative s... 26 9.5
>At3g21280.1 68416.m02689 ubiquitin-specific protease 7, putative
(UBP7) similar to GI:11993467
Length = 532
Score = 64.5 bits (150), Expect = 3e-11
Identities = 27/61 (44%), Positives = 42/61 (68%), Gaps = 1/61 (1%)
Frame = +2
Query: 71 VSVKVKWGKEMYPDVEVNTDDEPVLFKAQIFALTGVQPERQKVVCKGVTLRDDT-WGNFK 247
++V VKW K+++ +E++T P +FKAQ++ L+GV PERQK++ KG L+DD W
Sbjct: 57 LTVSVKWQKKVFESIEIDTSQPPFVFKAQLYDLSGVPPERQKIMVKGGLLKDDADWSTLG 116
Query: 248 L 250
L
Sbjct: 117 L 117
Score = 45.6 bits (103), Expect = 1e-05
Identities = 22/59 (37%), Positives = 32/59 (54%)
Frame = +1
Query: 256 NALVLVMGSKXXDVPAAPVEQTRFVEDMNXAELATAMDMPEGLINXGNTCYMNXTVQXL 432
N L+M ++ AP + F+ED+ + A + GL+N GNTCYMN T+Q L
Sbjct: 119 NGQKLMMMGTADEIVKAPEKGPVFMEDLPEEQQAANLGYSAGLVNLGNTCYMNSTMQCL 177
>At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative
(UBP6) similar to GI:11993465
Length = 482
Score = 60.1 bits (139), Expect = 6e-10
Identities = 28/56 (50%), Positives = 39/56 (69%)
Frame = +2
Query: 62 MPNVSVKVKWGKEMYPDVEVNTDDEPVLFKAQIFALTGVQPERQKVVCKGVTLRDD 229
MP VSVK W K++ +E++ P +FKAQ++ LTGV PERQK++ KG L+DD
Sbjct: 1 MPTVSVK--WQKKVLDGIEIDVSLPPYVFKAQLYDLTGVPPERQKIMVKGGLLKDD 54
Score = 52.8 bits (121), Expect = 1e-07
Identities = 26/63 (41%), Positives = 37/63 (58%)
Frame = +1
Query: 247 IDDNALVLVMGSKXXDVPAAPVEQTRFVEDMNXAELATAMDMPEGLINXGNTCYMNXTVQ 426
+ D +++MG+ ++ AP + F ED+ LAT + GL+N GNTCYMN TVQ
Sbjct: 62 VKDGQKLMMMGTAD-EIVKAPEKAIVFAEDLPEEALATNLGYSAGLVNLGNTCYMNSTVQ 120
Query: 427 XLK 435
LK
Sbjct: 121 CLK 123
>At5g49570.1 68418.m06135 transglutaminase-like family protein low
similarity to peptide:N-glycanase PNGase [Mus musculus]
GI:8347622; contains Pfam profile PF01841:
Transglutaminase-like superfamily
Length = 721
Score = 32.7 bits (71), Expect = 0.11
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +2
Query: 110 DVEVNTDDEPVLFKAQIFALTGVQPERQKVVCK 208
DV+ NT+D + + IF+LT V PE QK+V +
Sbjct: 16 DVDYNTEDGLEVLRFLIFSLTLVPPEEQKIVAE 48
>At5g42220.1 68418.m05139 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 879
Score = 32.3 bits (70), Expect = 0.15
Identities = 14/38 (36%), Positives = 26/38 (68%)
Frame = +2
Query: 116 EVNTDDEPVLFKAQIFALTGVQPERQKVVCKGVTLRDD 229
+VN ++ +LFK +I + TGV +Q+++ +G L+DD
Sbjct: 39 QVNKNETVLLFKEKIASETGVPVGQQRLIFRGRVLKDD 76
>At4g30890.2 68417.m04387 ubiquitin-specific protease 24, putative
(UBP24) identical to ubiquitin-specific protease 24
[Arabidopsis thaliana] GI:11993488
Length = 551
Score = 32.3 bits (70), Expect = 0.15
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +1
Query: 373 PEGLINXGNTCYMNXTVQXL 432
P GLIN GN C++N T+Q L
Sbjct: 196 PRGLINAGNLCFLNATLQAL 215
>At4g30890.1 68417.m04386 ubiquitin-specific protease 24, putative
(UBP24) identical to ubiquitin-specific protease 24
[Arabidopsis thaliana] GI:11993488
Length = 551
Score = 32.3 bits (70), Expect = 0.15
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +1
Query: 373 PEGLINXGNTCYMNXTVQXL 432
P GLIN GN C++N T+Q L
Sbjct: 196 PRGLINAGNLCFLNATLQAL 215
>At1g11980.1 68414.m01384 ubiquitin family protein similar to Chain
A, Structure Of Ubiquitin-Like Protein, Rub1 GI:6729726
from [Arabidopsis thaliana]
Length = 78
Score = 31.5 bits (68), Expect = 0.25
Identities = 17/53 (32%), Positives = 25/53 (47%)
Frame = +2
Query: 71 VSVKVKWGKEMYPDVEVNTDDEPVLFKAQIFALTGVQPERQKVVCKGVTLRDD 229
+ +KVK E D+E+ D K +I G+ P Q++V G L DD
Sbjct: 1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADD 53
>At4g06599.1 68417.m01024 ubiquitin family protein contains Pfam
domian PF00240: Ubiquitin family
Length = 340
Score = 31.1 bits (67), Expect = 0.34
Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +2
Query: 71 VSVKVKWGKEMYPDVEVNTDDEPVLFKAQIFALTGVQPERQKVVCK--GVTLRDDT 232
+++ VKW + Y V + DD K +I LT V P+RQK++ G L DD+
Sbjct: 24 LTLTVKWNGKEYT-VRICADDSVAELKRRICLLTTVLPKRQKLLYPKIGNKLSDDS 78
>At2g40930.1 68415.m05052 ubiquitin-specific protease 5, putative
(UBP5) similar to GI:6648604
Length = 924
Score = 31.1 bits (67), Expect = 0.34
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL+N GNTC+MN +Q L
Sbjct: 318 GLLNLGNTCFMNSAIQCL 335
>At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15)
almost identical to ubiquitin-specific protease 15
GI:11993475 [Arabidopsis thaliana], 7 amino acid
difference
Length = 924
Score = 31.1 bits (67), Expect = 0.34
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 373 PEGLINXGNTCYMNXTVQXL 432
P GL+N GN+CY N +Q L
Sbjct: 437 PRGLVNCGNSCYANAVLQSL 456
>At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase
family protein / zinc finger (MYND type) family protein
similar to ubiquitin-specific protease 16 (UBP16)
[Arabidopsis thaliana] GI:11993477; contains Pfam
profiles PF00443: Ubiquitin carboxyl-terminal hydrolase,
PF01753: MYND finger
Length = 731
Score = 30.7 bits (66), Expect = 0.44
Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 298 PAAPVEQTRFVEDMNXAELATAMDM-PEGLINXGNTCYMNXTVQXL 432
P++ +++ F ++ +++ P GL+N GN+CY N +Q L
Sbjct: 302 PSSSIDEMSFSYELFVKLYCDRVELQPFGLVNLGNSCYANAVLQCL 347
>At3g14400.1 68416.m01822 ubiquitin-specific protease 25 (UBP25)
similar to GI:11993490
Length = 661
Score = 30.3 bits (65), Expect = 0.59
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +1
Query: 373 PEGLINXGNTCYMNXTVQXL 432
P GL N GNTCY+N +Q L
Sbjct: 23 PLGLRNLGNTCYLNSVLQCL 42
>At1g32850.1 68414.m04048 ubiquitin carboxyl-terminal hydrolase
family protein similar to ubiquitin-specific protease
UBP5 [Arabidopsis thaliana] GI:6648604; contains Pfam
profile PF00443: Ubiquitin carboxyl-terminal hydrolase
Length = 892
Score = 30.3 bits (65), Expect = 0.59
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N GNTC+MN T+Q L
Sbjct: 302 GLQNLGNTCFMNSTLQCL 319
>At5g22030.2 68418.m02564 ubiquitin-specific protease 8, putative
(UBP8) similar to ubiquitin-specific protease 8 partial
sequence GI:11993469 [Arabidopsis thaliana]
Length = 622
Score = 28.7 bits (61), Expect = 1.8
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N GNTC+MN ++Q L
Sbjct: 31 GLQNLGNTCFMNSSLQCL 48
>At5g22030.1 68418.m02563 ubiquitin-specific protease 8, putative
(UBP8) similar to ubiquitin-specific protease 8 partial
sequence GI:11993469 [Arabidopsis thaliana]
Length = 622
Score = 28.7 bits (61), Expect = 1.8
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N GNTC+MN ++Q L
Sbjct: 31 GLQNLGNTCFMNSSLQCL 48
>At4g10590.2 68417.m01733 ubiquitin carboxyl-terminal hydrolase
family protein similar to ubiquitin-specific protease
UBP5 [Arabidopsis thaliana] GI:6648604; contains Pfam
profile PF00443: Ubiquitin carboxyl-terminal hydrolase
Length = 910
Score = 28.7 bits (61), Expect = 1.8
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N GNTC+MN +Q L
Sbjct: 304 GLSNLGNTCFMNSALQCL 321
>At4g10590.1 68417.m01732 ubiquitin carboxyl-terminal hydrolase
family protein similar to ubiquitin-specific protease
UBP5 [Arabidopsis thaliana] GI:6648604; contains Pfam
profile PF00443: Ubiquitin carboxyl-terminal hydrolase
Length = 910
Score = 28.7 bits (61), Expect = 1.8
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N GNTC+MN +Q L
Sbjct: 304 GLSNLGNTCFMNSALQCL 321
>At4g10570.1 68417.m01730 ubiquitin carboxyl-terminal hydrolase
family protein similar to ubiquitin-specific protease
UBP5 [Arabidopsis thaliana] GI:6648604; contains Pfam
profile PF00443: Ubiquitin carboxyl-terminal hydrolase
Length = 923
Score = 28.7 bits (61), Expect = 1.8
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N GNTC+MN +Q L
Sbjct: 305 GLSNLGNTCFMNSALQCL 322
>At2g32780.1 68415.m04013 ubiquitin-specific protease 1, putative
(UBP1) similar to GI:11993461
Length = 1083
Score = 28.7 bits (61), Expect = 1.8
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL+N GNTC+ N +Q L
Sbjct: 203 GLVNLGNTCFFNSVMQNL 220
>At1g04860.1 68414.m00482 ubiquitin-specific protease 2 (UBP2)
identical to GI:11993463
Length = 961
Score = 28.3 bits (60), Expect = 2.4
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL+N GNTC+ N +Q L
Sbjct: 232 GLVNLGNTCFFNSIMQNL 249
>At4g24560.1 68417.m03520 ubiquitin-specific protease 16, putative
(UBP16) similar to ubiquitin-specific protease 16
GI:11993477 [Arabidopsis thaliana]
Length = 1008
Score = 27.5 bits (58), Expect = 4.1
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1
Query: 373 PEGLINXGNTCYMNXTVQXL 432
P GLIN GN+C+ N Q L
Sbjct: 541 PCGLINVGNSCFANVVFQCL 560
>At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putative
(UBP23) identical to GI:11993486
Length = 859
Score = 27.1 bits (57), Expect = 5.5
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N GNTC++N +Q L
Sbjct: 108 GLQNLGNTCFLNSVLQCL 125
>At4g20700.1 68417.m03008 hypothetical protein
Length = 529
Score = 27.1 bits (57), Expect = 5.5
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 378 LWHIHGCSQFSXIHVFDKTCLFHWCCW 298
LWH G S FS + FD L+ C W
Sbjct: 20 LWH-RGTSSFSLLSCFDSLFLWFRCRW 45
>At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26)
similar to GI:11993492; RNA binding protein - Homo
sapiens, EMBL:AB016089 (N-terminus), several ubiquitin
carboxyl-terminal hydrolases from aa pos. 712
Length = 1067
Score = 27.1 bits (57), Expect = 5.5
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +1
Query: 373 PEGLINXGNTCYMNXTVQXL 432
P GL N G TCY N +Q L
Sbjct: 105 PAGLTNLGATCYANSILQCL 124
>At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putative
(UBP14) similar to ubiquitin-specific protease 14
GI:11993473 [Arabidopsis thaliana]
Length = 797
Score = 27.1 bits (57), Expect = 5.5
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQ 426
GL+N GN+CY+ T+Q
Sbjct: 309 GLVNLGNSCYLAATMQ 324
>At5g25010.1 68418.m02964 expressed protein ; expression supported
by MPSS
Length = 286
Score = 26.6 bits (56), Expect = 7.2
Identities = 13/32 (40%), Positives = 16/32 (50%), Gaps = 1/32 (3%)
Frame = +1
Query: 319 TRFVEDMNXA-ELATAMDMPEGLINXGNTCYM 411
T V D+ E T ++PE LI G CYM
Sbjct: 229 TNLVADVGFVVEAQTEEELPERLIGGGRVCYM 260
>At2g12550.1 68415.m01357 ubiquitin-associated (UBA)/TS-N
domain-containing protein low similarity to NUB1
(NEDD8-interacting protein) [Homo sapiens] GI:13383476;
contains Pfam profile PF00627: UBA/TS-N domain
Length = 562
Score = 26.6 bits (56), Expect = 7.2
Identities = 20/81 (24%), Positives = 40/81 (49%)
Frame = +2
Query: 62 MPNVSVKVKWGKEMYPDVEVNTDDEPVLFKAQIFALTGVQPERQKVVCKGVTLRDDTWGN 241
M + V WG + ++E T + + + ++ +G+ PE K++ G L+DD G+
Sbjct: 1 MAKLKVAGTWGGILEVELENWTLN---MLRDEVAKRSGLDPESIKLIFAGKILKDD--GD 55
Query: 242 FKLTITP*YLSWAVKXXTCQQ 304
LT++ + K +C+Q
Sbjct: 56 ATLTLSQLGIKENSKILSCRQ 76
>At5g10790.1 68418.m01254 ubiquitin-specific protease 22 (UBP22)
almost identical to ubiquitin-specific protease 22
GI:11993484 [Arabidopsis thaliana], one amino acid
difference
Length = 557
Score = 26.2 bits (55), Expect = 9.5
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N G+TC+MN +Q L
Sbjct: 178 GLNNLGSTCFMNAVLQAL 195
>At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12)
almost identical to ubiquitin-specific protease 12
GI:11993471 [Arabidopsis thaliana], one amino acid
difference
Length = 1115
Score = 26.2 bits (55), Expect = 9.5
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N G TCYMN +Q L
Sbjct: 199 GLKNQGATCYMNSLLQTL 216
>At5g06600.1 68418.m00745 ubiquitin-specific protease 12 (UBP12)
almost identical to ubiquitin-specific protease 12
GI:11993471 [Arabidopsis thaliana], one amino acid
difference
Length = 1116
Score = 26.2 bits (55), Expect = 9.5
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N G TCYMN +Q L
Sbjct: 200 GLKNQGATCYMNSLLQTL 217
>At3g11910.1 68416.m01460 ubiquitin-specific protease, putative
strong similarity to ubiquitin-specific protease 12
(UBP12) [Arabidopsis thaliana] GI:11993471; contains
Pfam profiles PF00443: Ubiquitin carboxyl-terminal
hydrolase, PF00917: MATH domain
Length = 1115
Score = 26.2 bits (55), Expect = 9.5
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +1
Query: 379 GLINXGNTCYMNXTVQXL 432
GL N G TCYMN +Q L
Sbjct: 199 GLKNQGATCYMNSLLQTL 216
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,239,332
Number of Sequences: 28952
Number of extensions: 178072
Number of successful extensions: 346
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 342
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 346
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 683042040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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