BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060643.seq (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H10.13 |zym1||metallothionein |Schizosaccharomyces pombe|c... 31 0.12 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.27 SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual 29 0.63 SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch... 27 1.9 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 27 2.5 SPCPJ732.01 |vps5||retromer complex subunit Vps5|Schizosaccharom... 27 2.5 SPAC20G8.10c ||SPAC3A12.01c|beclin family protein|Schizosaccharo... 27 2.5 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 27 2.5 SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosacch... 27 3.4 >SPAC22H10.13 |zym1||metallothionein |Schizosaccharomyces pombe|chr 1|||Manual Length = 50 Score = 31.5 bits (68), Expect = 0.12 Identities = 13/40 (32%), Positives = 16/40 (40%), Gaps = 1/40 (2%) Frame = -2 Query: 205 HAAPDSAPDGXLCDCLNTVGC-DCLNTVGCDCLKTVGCDC 89 H + G CDC + GC DC + GC C C Sbjct: 3 HTTQCKSKQGKPCDCQSKCGCQDCKESCGCKSSAVDNCKC 42 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 30.3 bits (65), Expect = 0.27 Identities = 14/25 (56%), Positives = 14/25 (56%) Frame = +3 Query: 96 QPTVFKQSQPTVFKQSQPTVFKQSQ 170 QPT F Q QPT F QP F Q Q Sbjct: 126 QPTGFVQPQPTGFMSQQPASFMQPQ 150 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/26 (50%), Positives = 13/26 (50%) Frame = +3 Query: 75 PAVFKQSQPTVFKQSQPTVFKQSQPT 152 P F Q QPT F QP F Q Q T Sbjct: 127 PTGFVQPQPTGFMSQQPASFMQPQRT 152 >SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual Length = 706 Score = 29.1 bits (62), Expect = 0.63 Identities = 15/27 (55%), Positives = 16/27 (59%) Frame = +3 Query: 90 QSQPTVFKQSQPTVFKQSQPTVFKQSQ 170 Q QPT F Q QPT F + Q T F Q Q Sbjct: 310 QPQPTGFLQQQPTGFIRPQNTGFVQPQ 336 >SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1092 Score = 27.5 bits (58), Expect = 1.9 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 3/50 (6%) Frame = +3 Query: 318 DPLLSQEDDTIESVDRASQQYQNSIASETAAQR---ALQRGLDLESQLMS 458 DP L Q+ ++ +A+QQ A + AAQ+ A Q+ ++E Q M+ Sbjct: 326 DPTLDQQLAAQQAAQQAAQQQAELAAQQAAAQQAQLAAQQAAEMERQRMA 375 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 27.1 bits (57), Expect = 2.5 Identities = 17/51 (33%), Positives = 26/51 (50%) Frame = -1 Query: 413 LRSCLRRNAILVLLRSTIHTFNGVIFLT*QRIVIANVNLTVHVIRDTLTMV 261 LRSC +VL IH G I T +R+V A+ L +I++ + +V Sbjct: 681 LRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLV 731 >SPCPJ732.01 |vps5||retromer complex subunit Vps5|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 27.1 bits (57), Expect = 2.5 Identities = 9/27 (33%), Positives = 16/27 (59%) Frame = +3 Query: 282 YHVHGQVNISNDDPLLSQEDDTIESVD 362 +H G S DP+L + DT++S++ Sbjct: 351 FHTSGSSKFSEQDPILIEAKDTLDSLE 377 >SPAC20G8.10c ||SPAC3A12.01c|beclin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 464 Score = 27.1 bits (57), Expect = 2.5 Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 1/50 (2%) Frame = -3 Query: 540 ESNKSGDCDNTHSTDKVDYKRETLGKFHSSVVTQDLNLAAEPSAQL-SPT 394 E N+SG+ D+ ++ DY + L + ++ D N P +L +PT Sbjct: 41 EMNESGESDDQMNSSSEDYPAQRLQLYKKTISEGDYNFDNVPPPELRTPT 90 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 27.1 bits (57), Expect = 2.5 Identities = 17/51 (33%), Positives = 26/51 (50%) Frame = -1 Query: 413 LRSCLRRNAILVLLRSTIHTFNGVIFLT*QRIVIANVNLTVHVIRDTLTMV 261 LRSC +VL IH G I T +R+V A+ L +I++ + +V Sbjct: 681 LRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLV 731 >SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosaccharomyces pombe|chr 2|||Manual Length = 490 Score = 26.6 bits (56), Expect = 3.4 Identities = 9/21 (42%), Positives = 12/21 (57%) Frame = -2 Query: 148 GCDCLNTVGCDCLKTVGCDCL 86 GC+C + GCD C+CL Sbjct: 259 GCNCSSLGGCDLNNPSRCECL 279 Score = 26.6 bits (56), Expect = 3.4 Identities = 9/20 (45%), Positives = 12/20 (60%) Frame = -2 Query: 169 CDCLNTVGCDCLNTVGCDCL 110 C+C + GCD N C+CL Sbjct: 260 CNCSSLGGCDLNNPSRCECL 279 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,717,041 Number of Sequences: 5004 Number of extensions: 25713 Number of successful extensions: 94 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 85 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 93 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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