BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060643.seq
(686 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22H10.13 |zym1||metallothionein |Schizosaccharomyces pombe|c... 31 0.12
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.27
SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual 29 0.63
SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch... 27 1.9
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 27 2.5
SPCPJ732.01 |vps5||retromer complex subunit Vps5|Schizosaccharom... 27 2.5
SPAC20G8.10c ||SPAC3A12.01c|beclin family protein|Schizosaccharo... 27 2.5
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 27 2.5
SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosacch... 27 3.4
>SPAC22H10.13 |zym1||metallothionein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 50
Score = 31.5 bits (68), Expect = 0.12
Identities = 13/40 (32%), Positives = 16/40 (40%), Gaps = 1/40 (2%)
Frame = -2
Query: 205 HAAPDSAPDGXLCDCLNTVGC-DCLNTVGCDCLKTVGCDC 89
H + G CDC + GC DC + GC C C
Sbjct: 3 HTTQCKSKQGKPCDCQSKCGCQDCKESCGCKSSAVDNCKC 42
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with
EF hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.3 bits (65), Expect = 0.27
Identities = 14/25 (56%), Positives = 14/25 (56%)
Frame = +3
Query: 96 QPTVFKQSQPTVFKQSQPTVFKQSQ 170
QPT F Q QPT F QP F Q Q
Sbjct: 126 QPTGFVQPQPTGFMSQQPASFMQPQ 150
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/26 (50%), Positives = 13/26 (50%)
Frame = +3
Query: 75 PAVFKQSQPTVFKQSQPTVFKQSQPT 152
P F Q QPT F QP F Q Q T
Sbjct: 127 PTGFVQPQPTGFMSQQPASFMQPQRT 152
>SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual
Length = 706
Score = 29.1 bits (62), Expect = 0.63
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +3
Query: 90 QSQPTVFKQSQPTVFKQSQPTVFKQSQ 170
Q QPT F Q QPT F + Q T F Q Q
Sbjct: 310 QPQPTGFLQQQPTGFIRPQNTGFVQPQ 336
>SPAC688.11 |end4|sla2|Huntingtin-interacting protein
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1092
Score = 27.5 bits (58), Expect = 1.9
Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = +3
Query: 318 DPLLSQEDDTIESVDRASQQYQNSIASETAAQR---ALQRGLDLESQLMS 458
DP L Q+ ++ +A+QQ A + AAQ+ A Q+ ++E Q M+
Sbjct: 326 DPTLDQQLAAQQAAQQAAQQQAELAAQQAAAQQAQLAAQQAAEMERQRMA 375
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 27.1 bits (57), Expect = 2.5
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = -1
Query: 413 LRSCLRRNAILVLLRSTIHTFNGVIFLT*QRIVIANVNLTVHVIRDTLTMV 261
LRSC +VL IH G I T +R+V A+ L +I++ + +V
Sbjct: 681 LRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLV 731
>SPCPJ732.01 |vps5||retromer complex subunit
Vps5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 576
Score = 27.1 bits (57), Expect = 2.5
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = +3
Query: 282 YHVHGQVNISNDDPLLSQEDDTIESVD 362
+H G S DP+L + DT++S++
Sbjct: 351 FHTSGSSKFSEQDPILIEAKDTLDSLE 377
>SPAC20G8.10c ||SPAC3A12.01c|beclin family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 464
Score = 27.1 bits (57), Expect = 2.5
Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -3
Query: 540 ESNKSGDCDNTHSTDKVDYKRETLGKFHSSVVTQDLNLAAEPSAQL-SPT 394
E N+SG+ D+ ++ DY + L + ++ D N P +L +PT
Sbjct: 41 EMNESGESDDQMNSSSEDYPAQRLQLYKKTISEGDYNFDNVPPPELRTPT 90
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 27.1 bits (57), Expect = 2.5
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = -1
Query: 413 LRSCLRRNAILVLLRSTIHTFNGVIFLT*QRIVIANVNLTVHVIRDTLTMV 261
LRSC +VL IH G I T +R+V A+ L +I++ + +V
Sbjct: 681 LRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLV 731
>SPBC428.08c |clr4||histone H3 methyltransferase
Clr4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 490
Score = 26.6 bits (56), Expect = 3.4
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = -2
Query: 148 GCDCLNTVGCDCLKTVGCDCL 86
GC+C + GCD C+CL
Sbjct: 259 GCNCSSLGGCDLNNPSRCECL 279
Score = 26.6 bits (56), Expect = 3.4
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = -2
Query: 169 CDCLNTVGCDCLNTVGCDCL 110
C+C + GCD N C+CL
Sbjct: 260 CNCSSLGGCDLNNPSRCECL 279
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,717,041
Number of Sequences: 5004
Number of extensions: 25713
Number of successful extensions: 94
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 93
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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