BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060642.seq (687 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 217 8e-59 AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 217 8e-59 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 209 2e-56 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 202 3e-54 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2 DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.7 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6 DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450 monoo... 23 3.6 AF388659-1|AAK71995.1| 782|Apis mellifera 1D-myo-inositol-trisp... 22 6.3 DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. 21 8.3 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 217 bits (530), Expect = 8e-59 Identities = 110/146 (75%), Positives = 120/146 (82%), Gaps = 3/146 (2%) Frame = +2 Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72 Query: 437 NGQTREHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSC 607 NGQTREHALLAFTLGVKQLIVGVNK P T +++ + S + ++ P+ Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYI--KKIGYNPAA 130 Query: 608 CRFSVPISGWHGDNMLELSTKMPWFK 685 F VPISGWHGDNMLE+S+KMPWFK Sbjct: 131 VAF-VPISGWHGDNMLEVSSKMPWFK 155 Score = 27.1 bits (57), Expect = 0.17 Identities = 11/12 (91%), Positives = 11/12 (91%) Frame = +1 Query: 223 WVLDKLKAERXR 258 WVLDKLKAER R Sbjct: 1 WVLDKLKAERER 12 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 217 bits (530), Expect = 8e-59 Identities = 110/146 (75%), Positives = 120/146 (82%), Gaps = 3/146 (2%) Frame = +2 Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129 Query: 437 NGQTREHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSC 607 NGQTREHALLAFTLGVKQLIVGVNK P T +++ + S + ++ P+ Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYI--KKIGYNPAA 187 Query: 608 CRFSVPISGWHGDNMLELSTKMPWFK 685 F VPISGWHGDNMLE+S+KMPWFK Sbjct: 188 VAF-VPISGWHGDNMLEVSSKMPWFK 212 Score = 145 bits (351), Expect = 4e-37 Identities = 67/69 (97%), Positives = 67/69 (97%) Frame = +1 Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 232 DKLKAERXR 258 DKLKAER R Sbjct: 61 DKLKAERER 69 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 209 bits (511), Expect = 2e-56 Identities = 106/142 (74%), Positives = 116/142 (81%), Gaps = 3/142 (2%) Frame = +2 Query: 269 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 448 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 449 REHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSCCRFS 619 REHALLAFTLGVKQLIVGVNK P T +++ + S + ++ P+ F Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYI--KKIGYNPAAVAF- 117 Query: 620 VPISGWHGDNMLELSTKMPWFK 685 VPISGWHGDNMLE+S+KMPWFK Sbjct: 118 VPISGWHGDNMLEVSSKMPWFK 139 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 202 bits (492), Expect = 3e-54 Identities = 104/146 (71%), Positives = 114/146 (78%), Gaps = 3/146 (2%) Frame = +2 Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436 GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISK Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129 Query: 437 NGQTREHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSC 607 NGQTREHALLAFTLGVKQLIVGVNK P +++ + S + ++ + Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYI--KKIGYNTAS 187 Query: 608 CRFSVPISGWHGDNMLELSTKMPWFK 685 F VPISGWHGDNMLE S K PW+K Sbjct: 188 VAF-VPISGWHGDNMLEPSPKTPWYK 212 Score = 145 bits (351), Expect = 4e-37 Identities = 67/69 (97%), Positives = 67/69 (97%) Frame = +1 Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 232 DKLKAERXR 258 DKLKAER R Sbjct: 61 DKLKAERER 69 Score = 41.5 bits (93), Expect = 7e-06 Identities = 19/21 (90%), Positives = 20/21 (95%) Frame = +3 Query: 555 IKKEVSSYIKKIGYNPAAVAF 617 IKKEVSSYIKKIGYN A+VAF Sbjct: 170 IKKEVSSYIKKIGYNTASVAF 190 Score = 32.7 bits (71), Expect = 0.003 Identities = 12/20 (60%), Positives = 17/20 (85%) Frame = +1 Query: 508 QMDSTEPPYSEPRFEESRRK 567 +MD T+PPYSE RFEE +++ Sbjct: 154 KMDMTDPPYSEARFEEIKKE 173 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 33.1 bits (72), Expect = 0.003 Identities = 16/35 (45%), Positives = 18/35 (51%) Frame = +2 Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412 VT +D PGH FI G D VL+VAA G Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229 Score = 25.4 bits (53), Expect = 0.51 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +1 Query: 64 KTHINIVVIGHVDSGKST 117 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.2 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +1 Query: 73 INIVVIGHVDSGKST 117 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monooxygenase protein. Length = 517 Score = 23.0 bits (47), Expect = 2.7 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = -2 Query: 65 FSLPIFG*SRITNCV*Y 15 FSLPIFG I +C+ Y Sbjct: 57 FSLPIFGTRWIFSCIGY 73 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 2.7 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 3.6 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450 monooxygenase protein. Length = 499 Score = 22.6 bits (46), Expect = 3.6 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = -2 Query: 260 YRSRSAFSLSNTQAYLKDPLPISWASFSN 174 Y +RS F L N +KD L ++ F+N Sbjct: 76 YATRSPFLLLNDPELIKDILIRDFSKFAN 104 Score = 22.6 bits (46), Expect = 3.6 Identities = 11/34 (32%), Positives = 16/34 (47%) Frame = +1 Query: 481 CQTAHRRSKQMDSTEPPYSEPRFEESRRKYPHTS 582 C ++ K D E Y + F+E+ R YP S Sbjct: 338 CPKNNKELKYDDIKEMEYLDKVFKETLRMYPPAS 371 >AF388659-1|AAK71995.1| 782|Apis mellifera 1D-myo-inositol-trisphosphate 3-kinaseisoform A protein. Length = 782 Score = 21.8 bits (44), Expect = 6.3 Identities = 10/36 (27%), Positives = 20/36 (55%) Frame = +1 Query: 484 QTAHRRSKQMDSTEPPYSEPRFEESRRKYPHTSRRL 591 ++ +RR+ + STE YS+ ++ + P +S L Sbjct: 371 ESENRRNSCLGSTETYYSKHNTQQFTQYIPESSSNL 406 >DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. Length = 495 Score = 21.4 bits (43), Expect = 8.3 Identities = 11/36 (30%), Positives = 18/36 (50%) Frame = -3 Query: 307 IVLASFELPESNIDCDTXHAQPLVCPIPKHI*RILY 200 IV+ FE+ +S D A IP ++ R+L+ Sbjct: 54 IVIGGFEIEKSEDDSFNNQADKSEKRIPLYVCRVLH 89 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 197,266 Number of Sequences: 438 Number of extensions: 4208 Number of successful extensions: 36 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 27 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 20952180 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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