Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060642.seq
(687 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 173 9e-44
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 173 9e-44
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 173 9e-44
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 173 9e-44
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 100 1e-21
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 95 3e-20
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 72 4e-13
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 69 4e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 38 0.005
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 38 0.005
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 38 0.006
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 38 0.006
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 36 0.033
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 36 0.033
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 35 0.044
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 35 0.058
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.18
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 33 0.18
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.23
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.23
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 0.72
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.7
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.7
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.7
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.2
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.2
At5g27540.1 68418.m03297 GTP-binding protein-related low similar... 28 5.0
At5g46380.1 68418.m05708 hypothetical protein 28 6.7
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 173 bits (421), Expect = 9e-44
Identities = 91/146 (62%), Positives = 106/146 (72%), Gaps = 3/146 (2%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSC 607
+GQTREHALLAFTLGVKQ+I NK P ++ + S L ++ P
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYL--KKVGYNPDK 187
Query: 608 CRFSVPISGWHGDNMLELSTKMPWFK 685
F VPISG+ GDNM+E ST + W+K
Sbjct: 188 IPF-VPISGFEGDNMIERSTNLDWYK 212
Score = 129 bits (312), Expect = 1e-30
Identities = 61/69 (88%), Positives = 62/69 (89%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERXR 258
DKLKAER R
Sbjct: 61 DKLKAERER 69
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 173 bits (421), Expect = 9e-44
Identities = 91/146 (62%), Positives = 106/146 (72%), Gaps = 3/146 (2%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSC 607
+GQTREHALLAFTLGVKQ+I NK P ++ + S L ++ P
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYL--KKVGYNPDK 187
Query: 608 CRFSVPISGWHGDNMLELSTKMPWFK 685
F VPISG+ GDNM+E ST + W+K
Sbjct: 188 IPF-VPISGFEGDNMIERSTNLDWYK 212
Score = 129 bits (312), Expect = 1e-30
Identities = 61/69 (88%), Positives = 62/69 (89%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERXR 258
DKLKAER R
Sbjct: 61 DKLKAERER 69
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 173 bits (421), Expect = 9e-44
Identities = 91/146 (62%), Positives = 106/146 (72%), Gaps = 3/146 (2%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSC 607
+GQTREHALLAFTLGVKQ+I NK P ++ + S L ++ P
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYL--KKVGYNPDK 187
Query: 608 CRFSVPISGWHGDNMLELSTKMPWFK 685
F VPISG+ GDNM+E ST + W+K
Sbjct: 188 IPF-VPISGFEGDNMIERSTNLDWYK 212
Score = 129 bits (312), Expect = 1e-30
Identities = 61/69 (88%), Positives = 62/69 (89%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERXR 258
DKLKAER R
Sbjct: 61 DKLKAERER 69
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 173 bits (421), Expect = 9e-44
Identities = 91/146 (62%), Positives = 106/146 (72%), Gaps = 3/146 (2%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKW---IPLNHHTVSPDLRNQEGSILIHQEDWLQPSC 607
+GQTREHALLAFTLGVKQ+I NK P ++ + S L ++ P
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYL--KKVGYNPDK 187
Query: 608 CRFSVPISGWHGDNMLELSTKMPWFK 685
F VPISG+ GDNM+E ST + W+K
Sbjct: 188 IPF-VPISGFEGDNMIERSTNLDWYK 212
Score = 129 bits (312), Expect = 1e-30
Identities = 61/69 (88%), Positives = 62/69 (89%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERXR 258
DKLKAER R
Sbjct: 61 DKLKAERER 69
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 100 bits (239), Expect = 1e-21
Identities = 48/93 (51%), Positives = 62/93 (66%)
Frame = +2
Query: 233 TN*RLSVXGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
TN + G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A G
Sbjct: 156 TNEEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKG 215
Query: 413 EFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
EFE G + GQTREH LA TLGV +LIV VNK
Sbjct: 216 EFETGYERGGQTREHVQLAKTLGVSKLIVVVNK 248
Score = 68.5 bits (160), Expect = 4e-12
Identities = 28/66 (42%), Positives = 47/66 (71%)
Frame = +1
Query: 61 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 240
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 241 KAERXR 258
+ ER +
Sbjct: 158 EEERLK 163
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 95.5 bits (227), Expect = 3e-20
Identities = 52/135 (38%), Positives = 80/135 (59%), Gaps = 2/135 (1%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG
Sbjct: 302 GITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDN 361
Query: 437 -NGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRNQE-GSILIHQEDWLQPSCC 610
GQTREHA + GV+Q+IV +NK + + DL Q GS L Q + S
Sbjct: 362 LKGQTREHARVLRGFGVEQVIVAINKMDIVGYSKERFDLIKQHVGSFL--QSCRFKDSSL 419
Query: 611 RFSVPISGWHGDNML 655
+ +P+S N++
Sbjct: 420 TW-IPLSAMENQNLV 433
Score = 77.0 bits (181), Expect = 1e-14
Identities = 32/64 (50%), Positives = 46/64 (71%)
Frame = +1
Query: 67 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 246
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 247 ERXR 258
ER R
Sbjct: 298 ERER 301
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 71.7 bits (168), Expect = 4e-13
Identities = 36/85 (42%), Positives = 52/85 (61%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G
Sbjct: 127 GITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP----- 181
Query: 437 NGQTREHALLAFTLGVKQLIVGVNK 511
QT+EH LLA +GV ++V +NK
Sbjct: 182 --QTKEHILLAKQVGVPDMVVFLNK 204
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 49 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 183
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 68.5 bits (160), Expect = 4e-12
Identities = 36/85 (42%), Positives = 51/85 (60%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 115 GITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP----- 169
Query: 437 NGQTREHALLAFTLGVKQLIVGVNK 511
QT+EH LLA +GV L+ +NK
Sbjct: 170 --QTKEHILLARQVGVPSLVCFLNK 192
Score = 32.7 bits (71), Expect = 0.23
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 58 KEKTHINIVVIGHVDSGKSTTT 123
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 38.3 bits (85), Expect = 0.005
Identities = 27/90 (30%), Positives = 38/90 (42%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 118 GITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITV 177
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLN 526
+ Q R + + K +G + W LN
Sbjct: 178 DRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 6.7
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 38.3 bits (85), Expect = 0.005
Identities = 27/90 (30%), Positives = 38/90 (42%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 118 GITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITV 177
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLN 526
+ Q R + + K +G + W LN
Sbjct: 178 DRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 6.7
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 37.9 bits (84), Expect = 0.006
Identities = 19/56 (33%), Positives = 27/56 (48%)
Frame = +2
Query: 245 LSVXGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
+S+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 190 ISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 37.9 bits (84), Expect = 0.006
Identities = 19/56 (33%), Positives = 27/56 (48%)
Frame = +2
Query: 245 LSVXGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
+S+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 190 ISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 35.5 bits (78), Expect = 0.033
Identities = 21/73 (28%), Positives = 35/73 (47%)
Frame = +2
Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 472
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 473 TLGVKQLIVGVNK 511
+ +K +I+ NK
Sbjct: 178 MMRLKHIIILQNK 190
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 35.5 bits (78), Expect = 0.033
Identities = 21/73 (28%), Positives = 35/73 (47%)
Frame = +2
Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 472
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 473 TLGVKQLIVGVNK 511
+ +K +I+ NK
Sbjct: 178 MMRLKHIIILQNK 190
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 35.1 bits (77), Expect = 0.044
Identities = 24/79 (30%), Positives = 37/79 (46%), Gaps = 4/79 (5%)
Frame = +2
Query: 287 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 454
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 455 HALLAFTLGVKQLIVGVNK 511
H + +K +I+ NK
Sbjct: 166 HLAAVEIMQLKHIIILQNK 184
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 34.7 bits (76), Expect = 0.058
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = +2
Query: 305 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 484
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 485 KQLIVGVNK 511
K +I+ NK
Sbjct: 174 KDIIIIQNK 182
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +2
Query: 290 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
E S Y + +ID PGH DF + S A+L+V A G
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG 171
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +1
Query: 40 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 159
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +2
Query: 260 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 60 ITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
Score = 32.7 bits (71), Expect = 0.23
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGG 150
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 33.1 bits (72), Expect = 0.18
Identities = 28/93 (30%), Positives = 40/93 (43%), Gaps = 1/93 (1%)
Frame = +2
Query: 317 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLI 496
+D PGH F G D A+++VAA G QT E A+ ++
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG-------IRPQTNE-AIAHAKAAAVPIV 608
Query: 497 VGVNKWIPLNHHTVSPDLRNQE-GSILIHQEDW 592
+ +NK ++ SPD QE SI + EDW
Sbjct: 609 IAINK---IDKEGASPDRVMQELSSIGLMPEDW 638
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.23
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCG 147
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
Score = 29.1 bits (62), Expect = 2.9
Identities = 17/52 (32%), Positives = 23/52 (44%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
GITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 146 GITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 31.1 bits (67), Expect = 0.72
Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = +2
Query: 257 GITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
GITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 133 GITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 29.1 bits (62), Expect = 2.9
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERX 255
N +I H+D GKST L+ G + R +++ + ++ + + KL+A R
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKEQFLDNMDLE----RERGITIKLQAARM 143
Query: 256 RY 261
RY
Sbjct: 144 RY 145
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 314 IIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 314 IIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 314 IIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +2
Query: 296 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 397
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 2.9
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2
Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At5g27540.1 68418.m03297 GTP-binding protein-related low similarity
to Mig-2-like GTPase Mtl [Drosophila melanogaster]
GI:7271872; contains Pfam profile PF00036: EF hand
Length = 648
Score = 28.3 bits (60), Expect = 5.0
Identities = 21/98 (21%), Positives = 41/98 (41%)
Frame = +2
Query: 296 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFT 475
++Y +D PG ++ ++ G + LIVAA T F + + + F
Sbjct: 2 ARYAAGAVDCPGSPKSVRIVVVGDKGTGKSSLIVAAATDSFPPNVPPVLPDYKLPIEFFP 61
Query: 476 LGVKQLIVGVNKWIPLNHHTVSPDLRNQEGSILIHQED 589
G+ IV + P + V+ +L+ + +L + D
Sbjct: 62 DGIPVTIVDTSS-RPEDRDIVAEELKRADAVVLTYACD 98
>At5g46380.1 68418.m05708 hypothetical protein
Length = 607
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -3
Query: 262 DTXHAQPLVCPIPKHI*RILYPFP-----GPPSRTSRW 164
D H +PLV P+P H R YP P GP +R+S +
Sbjct: 483 DAYHLRPLVAPLPLH--RDSYPCPPTLAAGPYTRSSAY 518
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,312,996
Number of Sequences: 28952
Number of extensions: 323115
Number of successful extensions: 973
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 911
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 958
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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