Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060637.seq
(555 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 148 6e-37
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 147 8e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 147 8e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 84 1e-17
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 83 4e-17
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 62 4e-11
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.005
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.005
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 31 0.15
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 30 0.20
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 27 2.5
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 27 2.5
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 3.2
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 26 4.3
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 7.5
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 7.5
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 25 7.5
SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine l... 25 9.9
SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.9
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 148 bits (358), Expect = 6e-37
Identities = 74/99 (74%), Positives = 81/99 (81%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET KY VT+IDAPG RDFIK MITGTSQADCAVLI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISK 129
Query: 438 NGQTREHALLAFXLGVKXLIRRSXXMGSTEPPYSEPRFE 554
+GQTREHALLA+ LGVK LI M +T +S+ RFE
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Score = 140 bits (338), Expect = 2e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 147 bits (357), Expect = 8e-37
Identities = 73/99 (73%), Positives = 81/99 (81%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET KY VT+IDAPG RDFIK MITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 438 NGQTREHALLAFXLGVKXLIRRSXXMGSTEPPYSEPRFE 554
+GQTREHALLA+ LGVK LI M +T +S+ RFE
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Score = 140 bits (338), Expect = 2e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 147 bits (357), Expect = 8e-37
Identities = 73/99 (73%), Positives = 81/99 (81%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET KY VT+IDAPG RDFIK MITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 438 NGQTREHALLAFXLGVKXLIRRSXXMGSTEPPYSEPRFE 554
+GQTREHALLA+ LGVK LI M +T +S+ RFE
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFE 166
Score = 140 bits (338), Expect = 2e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 84.2 bits (199), Expect = 1e-17
Identities = 40/99 (40%), Positives = 59/99 (59%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTGEFEAGISK 437
G T+++ FET +++DAPG + ++ MI G SQAD VL+++A GEFEAG +
Sbjct: 301 GKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFER 360
Query: 438 NGQTREHALLAFXLGVKXLIRRSXXMGSTEPPYSEPRFE 554
GQTREHA+LA G+ L+ M +SE R++
Sbjct: 361 GGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYK 399
Score = 79.4 bits (187), Expect = 3e-16
Identities = 34/64 (53%), Positives = 47/64 (73%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 244 AERD 255
ER+
Sbjct: 296 EERE 299
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 82.6 bits (195), Expect = 4e-17
Identities = 40/80 (50%), Positives = 50/80 (62%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTGEFEAGISK 437
G+T+D+A FE+ K I DAPG RDFI MI G S AD AVL+V + FE G +
Sbjct: 240 GVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLE 299
Query: 438 NGQTREHALLAFXLGVKXLI 497
NGQTREHA L LG+ ++
Sbjct: 300 NGQTREHAYLLRALGISEIV 319
Score = 72.5 bits (170), Expect = 4e-14
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 244 AER 252
ER
Sbjct: 235 EER 237
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 62.5 bits (145), Expect = 4e-11
Identities = 36/95 (37%), Positives = 52/95 (54%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITI A ++ET+ + +D PG D+IK MITG + D A+++V+A G+
Sbjct: 101 GITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP----- 155
Query: 438 NGQTREHALLAFXLGVKXLIRRSXXMGSTEPPYSE 542
QTREH LLA +GVK ++ + EP E
Sbjct: 156 --QTREHLLLARQVGVKQIVVYINKVDMVEPDMIE 188
Score = 35.1 bits (77), Expect = 0.007
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 58 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 144
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.005
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.005
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 30.7 bits (66), Expect = 0.15
Identities = 17/52 (32%), Positives = 23/52 (44%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTG 413
GITI+ A F + +ID PG DF + + D AV I+ G
Sbjct: 78 GITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
Score = 28.7 bits (61), Expect = 0.61
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 30.3 bits (65), Expect = 0.20
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = +3
Query: 288 FETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 458
FE S Y + IID PG DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
Score = 29.1 bits (62), Expect = 0.46
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 26.6 bits (56), Expect = 2.5
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +3
Query: 315 IIDAPGXRDFIKXMITGTSQADCAVLIV 398
IID PG F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 26.6 bits (56), Expect = 2.5
Identities = 11/45 (24%), Positives = 22/45 (48%)
Frame = +3
Query: 279 LWKFETSKYYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTG 413
++ + Y + +ID PG DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
Score = 24.6 bits (51), Expect = 9.9
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGIDK 159
N VI H+D GKST + ++ G I++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 26.2 bits (55), Expect = 3.2
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +3
Query: 303 YYVTIIDAPGXRDFIKXMITGTSQADCAVLIVAAGTG 413
Y + +ID+PG DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 4.3
Identities = 18/61 (29%), Positives = 25/61 (40%)
Frame = -3
Query: 292 SNFQRAISIVIPSHAQPLVCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWW 113
SN +R ++ V +PK I I++ F P S S C+Y S D W
Sbjct: 13 SNLRRMNRDHSSNNTNRTVLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWR 72
Query: 112 T 110
T
Sbjct: 73 T 73
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 46 PKMGKEKTHINIVVIGHVDSGKST 117
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 7.5
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -3
Query: 193 PGPPSRTSRWYVCQYHHIC 137
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 25.0 bits (52), Expect = 7.5
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -2
Query: 326 SINDGNIVLASFELPESNIDCDTXSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIP 147
S++ + L S+ P S T A ++YL+ PL S S S + +R SIP
Sbjct: 305 SVDSASTALNSYSTPNSVSRKLTNLTPAARRLVARSYLRSPLHGSSPSASRHTALRTSIP 364
>SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine
ligase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 788
Score = 24.6 bits (51), Expect = 9.9
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = -3
Query: 274 ISIVIPSHAQPLVCPIPKHI*RILYPFPGPPSRTSR 167
+++VIP PLV I H R+ P GP + +R
Sbjct: 71 VNLVIPGPELPLVEGIEGHFRRVGIPCFGPSALAAR 106
>SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 96
Score = 24.6 bits (51), Expect = 9.9
Identities = 12/26 (46%), Positives = 13/26 (50%)
Frame = -2
Query: 362 CDHXLDEISXSRSINDGNIVLASFEL 285
CDH L IS SR G + FEL
Sbjct: 23 CDHLLQYISNSRDSKAGLKIFLVFEL 48
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,066,242
Number of Sequences: 5004
Number of extensions: 37447
Number of successful extensions: 124
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 231978230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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