BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060631.seq (541 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g08280.1 68418.m00975 hydroxymethylbilane synthase / porphobi... 66 2e-11 At3g10650.1 68416.m01281 expressed protein 29 2.0 At4g28410.1 68417.m04067 aminotransferase-related similar to nic... 27 6.1 At1g53350.1 68414.m06048 disease resistance protein (CC-NBS-LRR ... 27 8.0 >At5g08280.1 68418.m00975 hydroxymethylbilane synthase / porphobilinogen deaminase, chloroplast / pre-uroporphyrinogen synthase identical to SP|Q43316 Length = 382 Score = 65.7 bits (153), Expect = 2e-11 Identities = 33/85 (38%), Positives = 51/85 (60%), Gaps = 4/85 (4%) Frame = +2 Query: 254 VALVQTNFVIDSLKRNYPEK----EFKIVTMTTLGDRVLDQPLPXIGEKSLXTKXLXDAL 421 +AL Q + LK+ +PE I + T GD++L QPL IG K L TK + +AL Sbjct: 84 LALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEAL 143 Query: 422 MSKNVDFVVHSLKDLPTTLPDGLVI 496 ++ ++D VHS+KD+PT LP+ ++ Sbjct: 144 INGHIDIAVHSMKDVPTYLPEKTIL 168 >At3g10650.1 68416.m01281 expressed protein Length = 1309 Score = 29.1 bits (62), Expect = 2.0 Identities = 21/57 (36%), Positives = 28/57 (49%), Gaps = 1/57 (1%) Frame = -2 Query: 501 APMTXP-SGNVVGKSFXECTTKSTFLLISASXKXFVXSDFSPIXGRG*SSTLSPSVV 334 AP + P SG V SF +T +TF S V S +PI G ++T SPS + Sbjct: 1095 APNSSPNSGPVFSSSFTTSSTPTTFSF-GGSSAATVSSTTTPIFGASTNNTPSPSPI 1150 >At4g28410.1 68417.m04067 aminotransferase-related similar to nicotianamine aminotransferase [Hordeum vulgare subsp. vulgare] GI:6469090 Length = 447 Score = 27.5 bits (58), Expect = 6.1 Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 1/40 (2%) Frame = +2 Query: 353 VLDQPLPXIGEKSLXTKX-LXDALMSKNVDFVVHSLKDLP 469 +L LP I EK+ ++++S+NVDF +LKD+P Sbjct: 321 ILQFALPNILEKTKKEFFEKNNSILSQNVDFAFDALKDIP 360 >At1g53350.1 68414.m06048 disease resistance protein (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists, suggestive of a disease resistance protein. Length = 906 Score = 27.1 bits (57), Expect = 8.0 Identities = 11/33 (33%), Positives = 22/33 (66%) Frame = +3 Query: 75 SVGDMLSQK*LNLFKKNDSNKVTSTSNLKNIIC 173 S+GD++ + L+L++ S+ +S NLK ++C Sbjct: 594 SIGDLIHLRFLSLYEAGVSHLPSSLGNLKLLLC 626 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,295,391 Number of Sequences: 28952 Number of extensions: 155856 Number of successful extensions: 269 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 267 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 269 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1003808112 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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