BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060630.seq
(551 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosacchar... 65 7e-12
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 33 0.028
SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A related|Schizosa... 30 0.20
SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha... 29 0.60
SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|... 25 5.6
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 7.4
>SPBP8B7.16c |dbp2||ATP-dependent RNA helicase
Dbp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 64.9 bits (151), Expect = 7e-12
Identities = 47/136 (34%), Positives = 65/136 (47%)
Frame = +1
Query: 121 VAPDWDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRXNHEVTVSGLRFIILFNTLKKQIF 300
V DW + +L PF K+FY H V RS EV EYR E+ V GL T ++ F
Sbjct: 70 VKKDWKNETLIPFQKDFYKEHENVRNRSDAEVTEYRKEKEIVVHGLNVPKPVTTFEEAGF 129
Query: 301 LIMCNKV*RQWVTKNPTLFKLKAGPIAMSGKXI*FGVXKTGFRQKRXAYILPXXCAP*TN 480
K +Q + PT + +A P+AMSG+ + G+ TG K +Y LP
Sbjct: 130 PNYVLKEVKQLGFEAPTPIQQQAWPMAMSGRDM-VGISATG-SGKTLSYCLP-AIVHINA 186
Query: 481 QPXIXRXDGRLLWVXA 528
QP + DG ++ V A
Sbjct: 187 QPLLSPGDGPIVLVLA 202
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 33.1 bits (72), Expect = 0.028
Identities = 13/34 (38%), Positives = 23/34 (67%)
Frame = +3
Query: 258 EVHNPIQYFEEANFPDYVQQGVKTMGYKEPDAIQ 359
++ NP++ +EEA P + + +K + YKEP +IQ
Sbjct: 243 DLPNPLRNWEEAGLPSEMLKVLKKVNYKEPSSIQ 276
>SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A
related|Schizosaccharomyces pombe|chr 1|||Manual
Length = 394
Score = 30.3 bits (65), Expect = 0.20
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +3
Query: 267 NPIQYFEEANFPDYVQQGVKTMGYKEPDAIQAQ 365
N + FEE N + + +G+ GY+ P A+Q++
Sbjct: 18 NAVSSFEEMNLKEDLLRGIYAYGYETPSAVQSR 50
>SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 578
Score = 28.7 bits (61), Expect = 0.60
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 270 PIQYFEEANFPDYVQQGVKTMGYKEPDAIQAQGW 371
PI F+E + +++G+K YKEP IQA W
Sbjct: 165 PILQFDELDVSAKLREGLKN--YKEPTPIQAATW 196
>SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 5.6
Identities = 12/22 (54%), Positives = 17/22 (77%), Gaps = 2/22 (9%)
Frame = -3
Query: 333 PLSLHLVAHNQENL--LLQSIE 274
PL LH+V+HN+++L LQ IE
Sbjct: 217 PLYLHIVSHNRKDLTVALQEIE 238
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 25.0 bits (52), Expect = 7.4
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 270 PIQYFEEANFPDYVQQGVKTMGYKEPDAIQAQGWADSYV 386
P+Q + E+ FP V Q T+G DA+ + +Y+
Sbjct: 1148 PLQQYAESGFPSEVDQASCTVGQDLLDAMSILRFLVAYL 1186
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,970,408
Number of Sequences: 5004
Number of extensions: 35575
Number of successful extensions: 88
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 86
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -