BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060630.seq (551 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosacchar... 65 7e-12 SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 33 0.028 SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A related|Schizosa... 30 0.20 SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha... 29 0.60 SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|... 25 5.6 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 7.4 >SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 64.9 bits (151), Expect = 7e-12 Identities = 47/136 (34%), Positives = 65/136 (47%) Frame = +1 Query: 121 VAPDWDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRXNHEVTVSGLRFIILFNTLKKQIF 300 V DW + +L PF K+FY H V RS EV EYR E+ V GL T ++ F Sbjct: 70 VKKDWKNETLIPFQKDFYKEHENVRNRSDAEVTEYRKEKEIVVHGLNVPKPVTTFEEAGF 129 Query: 301 LIMCNKV*RQWVTKNPTLFKLKAGPIAMSGKXI*FGVXKTGFRQKRXAYILPXXCAP*TN 480 K +Q + PT + +A P+AMSG+ + G+ TG K +Y LP Sbjct: 130 PNYVLKEVKQLGFEAPTPIQQQAWPMAMSGRDM-VGISATG-SGKTLSYCLP-AIVHINA 186 Query: 481 QPXIXRXDGRLLWVXA 528 QP + DG ++ V A Sbjct: 187 QPLLSPGDGPIVLVLA 202 >SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 33.1 bits (72), Expect = 0.028 Identities = 13/34 (38%), Positives = 23/34 (67%) Frame = +3 Query: 258 EVHNPIQYFEEANFPDYVQQGVKTMGYKEPDAIQ 359 ++ NP++ +EEA P + + +K + YKEP +IQ Sbjct: 243 DLPNPLRNWEEAGLPSEMLKVLKKVNYKEPSSIQ 276 >SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A related|Schizosaccharomyces pombe|chr 1|||Manual Length = 394 Score = 30.3 bits (65), Expect = 0.20 Identities = 11/33 (33%), Positives = 20/33 (60%) Frame = +3 Query: 267 NPIQYFEEANFPDYVQQGVKTMGYKEPDAIQAQ 365 N + FEE N + + +G+ GY+ P A+Q++ Sbjct: 18 NAVSSFEEMNLKEDLLRGIYAYGYETPSAVQSR 50 >SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 578 Score = 28.7 bits (61), Expect = 0.60 Identities = 14/34 (41%), Positives = 20/34 (58%) Frame = +3 Query: 270 PIQYFEEANFPDYVQQGVKTMGYKEPDAIQAQGW 371 PI F+E + +++G+K YKEP IQA W Sbjct: 165 PILQFDELDVSAKLREGLKN--YKEPTPIQAATW 196 >SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 534 Score = 25.4 bits (53), Expect = 5.6 Identities = 12/22 (54%), Positives = 17/22 (77%), Gaps = 2/22 (9%) Frame = -3 Query: 333 PLSLHLVAHNQENL--LLQSIE 274 PL LH+V+HN+++L LQ IE Sbjct: 217 PLYLHIVSHNRKDLTVALQEIE 238 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 25.0 bits (52), Expect = 7.4 Identities = 12/39 (30%), Positives = 20/39 (51%) Frame = +3 Query: 270 PIQYFEEANFPDYVQQGVKTMGYKEPDAIQAQGWADSYV 386 P+Q + E+ FP V Q T+G DA+ + +Y+ Sbjct: 1148 PLQQYAESGFPSEVDQASCTVGQDLLDAMSILRFLVAYL 1186 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,970,408 Number of Sequences: 5004 Number of extensions: 35575 Number of successful extensions: 88 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 86 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 87 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 229961028 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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