BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060619.seq (695 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 139 3e-34 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 132 6e-32 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 117 2e-27 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 110 2e-25 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 82 6e-17 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 49 6e-07 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 1e-04 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.039 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.84 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 5.9 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.8 SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 25 7.8 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 139 bits (337), Expect = 3e-34 Identities = 63/86 (73%), Positives = 74/86 (86%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435 NP+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SS Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 MVL KM+ETAEAYLG V +AV+TVP Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVP 145 Score = 107 bits (257), Expect = 1e-24 Identities = 49/58 (84%), Positives = 53/58 (91%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 Score = 79.4 bits (187), Expect = 5e-16 Identities = 43/61 (70%), Positives = 47/61 (77%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGSLTKXGYWENEIVLIF*TSGXR 689 PAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYG L + E+ VLIF G Sbjct: 145 PAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYG-LDRSNQGESN-VLIFDLGGGT 202 Query: 690 Y 692 + Sbjct: 203 F 203 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 132 bits (318), Expect = 6e-32 Identities = 59/86 (68%), Positives = 72/86 (83%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435 NP+NTIFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SS Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 MVL KM+E+AEA+LG V +AV+TVP Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVP 145 Score = 107 bits (257), Expect = 1e-24 Identities = 49/58 (84%), Positives = 53/58 (91%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 Score = 79.0 bits (186), Expect = 6e-16 Identities = 43/61 (70%), Positives = 46/61 (75%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGSLTKXGYWENEIVLIF*TSGXR 689 PAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYG L + E VLIF G Sbjct: 145 PAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYG-LDRSNQHETN-VLIFDLGGGT 202 Query: 690 Y 692 + Sbjct: 203 F 203 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 117 bits (281), Expect = 2e-27 Identities = 53/86 (61%), Positives = 65/86 (75%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435 NP NT+FDAKRLIGR++ED Q D+KHWPF+V+ G P I+V Y GE K F +E+S+ Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISA 123 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 MVLTKMKE +EA L K V+ AVITVP Sbjct: 124 MVLTKMKEISEAKLNKRVEKAVITVP 149 Score = 95.5 bits (227), Expect = 6e-21 Identities = 43/58 (74%), Positives = 49/58 (84%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A P Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65 Score = 75.4 bits (177), Expect = 7e-15 Identities = 41/61 (67%), Positives = 45/61 (73%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGSLTKXGYWENEIVLIF*TSGXR 689 PAYF+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYG K +N VLIF G Sbjct: 149 PAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKN--VLIFDLGGGT 206 Query: 690 Y 692 + Sbjct: 207 F 207 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 110 bits (265), Expect = 2e-25 Identities = 49/86 (56%), Positives = 67/86 (77%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435 NP NTIFD KRLIGRKF++ T+ D+K +PF +V+ +P ++V G+ K F PEE+S+ Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 M+L+KMK+TAEAYLGK V +AV+TVP Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVP 178 Score = 95.9 bits (228), Expect = 5e-21 Identities = 42/57 (73%), Positives = 49/57 (85%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNP 94 Score = 77.4 bits (182), Expect = 2e-15 Identities = 34/38 (89%), Positives = 38/38 (100%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYG 623 PAYFND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYG Sbjct: 178 PAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYG 215 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 82.2 bits (194), Expect = 6e-17 Identities = 38/86 (44%), Positives = 54/86 (62%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVSS 435 NP NT F KRLIGR+F++ VQ D+K P+++V + KT+ P ++ Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGG 165 Query: 436 MVLTKMKETAEAYLGKTVQNAVITVP 513 +L+KM+ETA YLGK V+NAV+TVP Sbjct: 166 FILSKMRETASTYLGKDVKNAVVTVP 191 Score = 72.9 bits (171), Expect = 4e-14 Identities = 32/38 (84%), Positives = 36/38 (94%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYG 623 PAYFNDSQRQATK AG I+GLNVLR++NEPTAAA+AYG Sbjct: 191 PAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYG 228 Score = 69.3 bits (162), Expect = 5e-13 Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%) Frame = +2 Query: 80 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAR 256 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 257 TPTTQY 274 P + Sbjct: 109 NPENTF 114 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 49.2 bits (112), Expect = 6e-07 Identities = 20/59 (33%), Positives = 38/59 (64%) Frame = +2 Query: 77 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61 Score = 47.2 bits (107), Expect = 2e-06 Identities = 24/49 (48%), Positives = 33/49 (67%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGSLTKXGYWENE 656 PA+F D QR+A +A I+GLN LR++N+ AAA+ YG +TK E E Sbjct: 149 PAWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYG-ITKTDLPEPE 196 Score = 41.9 bits (94), Expect = 8e-05 Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 1/87 (1%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSXGGKPKIKVAYKGEDKTFFPEEVS 432 N NT+ KRL GR ++D ++ + ++ G KV Y E+ F ++ Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122 Query: 433 SMVLTKMKETAEAYLGKTVQNAVITVP 513 + TK+K AEA L +V + VI++P Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISIP 149 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/55 (30%), Positives = 31/55 (56%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAR 256 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ R Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVR 81 Score = 39.5 bits (88), Expect = 4e-04 Identities = 17/41 (41%), Positives = 26/41 (63%) Frame = +3 Query: 495 CSYHGPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIA 617 C P YF D+QR+A + A +GL VL++I++P A +A Sbjct: 166 CVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILA 206 Score = 32.3 bits (70), Expect = 0.068 Identities = 22/92 (23%), Positives = 45/92 (48%), Gaps = 6/92 (6%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXGGKPKIKVAYKGED------KTFF 417 N +N++ + + L+G+ ++ T+ H+ V+ G+ K+ + ++ K Sbjct: 82 NADNSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDEESDPKEKILT 139 Query: 418 PEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 513 E S L ++ E+AE +LG V V++VP Sbjct: 140 AHEASVRHLRRLTESAEDFLGTKVNGCVMSVP 171 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 33.1 bits (72), Expect = 0.039 Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTPTT 268 + ID GT ++ + + G +EI+ R S VAF ER+ G A N R P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83 Query: 269 QYSMPNV 289 +S+ NV Sbjct: 84 -HSIRNV 89 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.84 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 84 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 179 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/53 (30%), Positives = 26/53 (49%) Frame = -1 Query: 170 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 12 G W SP S + H ++++ +F +RVL C+ L K +K+A L Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/34 (35%), Positives = 17/34 (50%) Frame = +2 Query: 200 AFTDTERLIGDAAKNQVARTPTTQYSMPNVSSDV 301 AF D + G AK A P+T+ + N+S V Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188 >SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 25.4 bits (53), Expect = 7.8 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = -2 Query: 262 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 113 WGS +G + TLG+ + + + L+VGD LHL V ++ +A +R Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,019,634 Number of Sequences: 5004 Number of extensions: 66024 Number of successful extensions: 233 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 210 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 232 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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