Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060610.seq
(628 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 166 2e-42
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 96 3e-21
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 92 7e-20
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 68 1e-12
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 50 4e-07
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 44 2e-05
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 40 4e-04
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 35 0.011
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 27 1.7
SPBC651.09c |||RNA polymerase II associated Paf1 complex |Schizo... 25 6.8
SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces... 25 6.8
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 166 bits (404), Expect = 2e-42
Identities = 88/134 (65%), Positives = 94/134 (70%)
Frame = +1
Query: 7 SPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASXXXXXXXXXXX 186
+PEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQAVLSLYAS
Sbjct: 97 APEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSG 156
Query: 187 XXCLPHRAHLRRIRTPPRHPASGLAGXDLTDYLMKILTERGYSFTTTAEREIVRDIKEKL 366
P LAG DLTDYLMKIL ERGY+F+TTAEREIVRDIKEKL
Sbjct: 157 DGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKL 216
Query: 367 WYVALDFEQEMXTA 408
YVALDFEQE+ TA
Sbjct: 217 CYVALDFEQELQTA 230
Score = 77.8 bits (183), Expect = 1e-15
Identities = 37/54 (68%), Positives = 39/54 (72%)
Frame = +3
Query: 423 LEKSYELPDGQVITXGNXRFRCXEVLFQPCSWVWKLAGFHXTTYXSIXKCDVDI 584
LEKSYELPDGQVIT GN RFR E LFQP + + AG H TY SI KCDVDI
Sbjct: 236 LEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDI 289
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 96.3 bits (229), Expect = 3e-21
Identities = 54/133 (40%), Positives = 73/133 (54%), Gaps = 1/133 (0%)
Frame = +1
Query: 4 TSPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASXXXXXXXXXX 183
T PEEHP+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS
Sbjct: 101 TLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLEC 160
Query: 184 XXXCLPHRAHL-RRIRTPPRHPASGLAGXDLTDYLMKILTERGYSFTTTAEREIVRDIKE 360
L H + P + G D+TDYL L + G+ ++AE+EIVR+IKE
Sbjct: 161 GDG-LTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKE 219
Query: 361 KLWYVALDFEQEM 399
K YVA DF E+
Sbjct: 220 KCCYVASDFRSEI 232
Score = 42.3 bits (95), Expect = 6e-05
Identities = 20/51 (39%), Positives = 27/51 (52%)
Frame = +3
Query: 432 SYELPDGQVITXGNXRFRCXEVLFQPCSWVWKLAGFHXTTYXSIXKCDVDI 584
+Y+LPD Q IT G F EVLF P + +G H + SI D+D+
Sbjct: 244 TYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDL 294
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 91.9 bits (218), Expect = 7e-20
Identities = 52/127 (40%), Positives = 67/127 (52%)
Frame = +1
Query: 10 PEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASXXXXXXXXXXXX 189
P +LLTE P+NP ANREKM + MFE + +YVAIQAVLSLYA
Sbjct: 98 PRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGD 157
Query: 190 XCLPHRAHLRRIRTPPRHPASGLAGXDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLW 369
+ +AG D T YL+ +L +GY+F TA+ E VR++KEKL
Sbjct: 158 GVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLC 217
Query: 370 YVALDFE 390
YV+ D E
Sbjct: 218 YVSYDLE 224
Score = 44.0 bits (99), Expect = 2e-05
Identities = 22/54 (40%), Positives = 30/54 (55%)
Frame = +3
Query: 423 LEKSYELPDGQVITXGNXRFRCXEVLFQPCSWVWKLAGFHXTTYXSIXKCDVDI 584
L ++Y LPDG+VI G+ R+ C E LFQP + G + +I DVDI
Sbjct: 236 LMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDI 289
Score = 37.5 bits (83), Expect = 0.002
Identities = 15/26 (57%), Positives = 19/26 (73%)
Frame = +2
Query: 176 LDSGDGVSHTVPIYEGYALPHAILRL 253
+DSGDGV+H VP+YE L H + RL
Sbjct: 153 VDSGDGVTHIVPVYESVVLNHLVGRL 178
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 67.7 bits (158), Expect = 1e-12
Identities = 58/173 (33%), Positives = 80/173 (46%), Gaps = 10/173 (5%)
Frame = +1
Query: 10 PEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASXXXXXXXXXXXX 189
PE+H LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS
Sbjct: 121 PEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLT 180
Query: 190 XCLPHR----AHLRRIRTPPRHPAS----GLAGXDLTDYLMKILTERGY--SFTTTAERE 339
+ H+ + +S LAG D+T ++ +L +R S TAER
Sbjct: 181 GTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER- 239
Query: 340 IVRDIKEKLWYVALDFEQEMXTAYPAAPSRSLTNFPTVRSSLSETXDSVAQRF 498
IKE+ YV D +E + + P R L + S T D +RF
Sbjct: 240 ----IKEECCYVCPDIVKEF-SRFDREPDRYLKYASESITGHSTTIDVGFERF 287
Score = 33.9 bits (74), Expect = 0.019
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = +2
Query: 176 LDSGDGVSHTVPIYEGYALPHAI 244
+DSGDGV+H +P+ EGY + +I
Sbjct: 184 VDSGDGVTHIIPVAEGYVIGSSI 206
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 49.6 bits (113), Expect = 4e-07
Identities = 23/50 (46%), Positives = 31/50 (62%)
Frame = +1
Query: 4 TSPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAS 153
T+P EHP+L+TE NP NR K + MFE+ PA Y+A Q + +AS
Sbjct: 94 TNPLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFAS 143
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 426 EKSYELPDGQVITXGNXRFRCXEVLFQPCS 515
E +E PDG + G R++ E LF P S
Sbjct: 261 ESEFEFPDGSRMMFGAERYQIPEHLFVPGS 290
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 43.6 bits (98), Expect = 2e-05
Identities = 18/49 (36%), Positives = 27/49 (55%)
Frame = +1
Query: 1 GTSPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLY 147
G EHP+ +TE NP R MT+++FE +N P++ I + S Y
Sbjct: 110 GIDRMEHPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFY 158
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 39.5 bits (88), Expect = 4e-04
Identities = 16/49 (32%), Positives = 31/49 (63%)
Frame = +1
Query: 7 SPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAS 153
+P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+
Sbjct: 98 NPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFAN 146
Score = 35.1 bits (77), Expect = 0.008
Identities = 21/61 (34%), Positives = 26/61 (42%), Gaps = 9/61 (14%)
Frame = +3
Query: 429 KSYELPDGQVITXGNXRFRCXEVLFQPCSWVWKLA---------GFHXTTYXSIXKCDVD 581
K +E PDG G RFR E+LF P + A G H Y SI CD +
Sbjct: 279 KPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSE 338
Query: 582 I 584
+
Sbjct: 339 L 339
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 34.7 bits (76), Expect = 0.011
Identities = 18/58 (31%), Positives = 34/58 (58%)
Frame = +1
Query: 256 LAGXDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLWYVALDFEQEMXTAYPAAPSR 429
+ G LT+YL ++++ R Y+ E IV +IKE + YV+ +F+++M + S+
Sbjct: 188 VGGKLLTNYLKEVISYRKYNMME--ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSK 243
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 27.5 bits (58), Expect = 1.7
Identities = 14/35 (40%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +1
Query: 1 GTSPEEHPVLLTE-APLNPKANREKMTQIMFETFN 102
G PEE + E APL+ + +KM +++FET+N
Sbjct: 171 GPIPEEDFFRVLETAPLHAEEVSKKMKELLFETYN 205
>SPBC651.09c |||RNA polymerase II associated Paf1 complex
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 560
Score = 25.4 bits (53), Expect = 6.8
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +1
Query: 1 GTSPEEHPVLLTEAPLNPKANREKMTQIMFETFNTP 108
GTSP+ P L T PK N + + E ++P
Sbjct: 468 GTSPQPSPSLSTSIMSTPKLNPSESVVVASEKASSP 503
>SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 565
Score = 25.4 bits (53), Expect = 6.8
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +3
Query: 180 TPATVSPTPCPSTKDTHSPTPSCVWLSRXRPHRLPHEDPH 299
T AT + P STK+ +P P L +RL H+ H
Sbjct: 5 TTATTTGKPSRSTKNPDAPRPYKCPLCTKAFYRLEHQTRH 44
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,331,714
Number of Sequences: 5004
Number of extensions: 42315
Number of successful extensions: 130
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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