Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060601.seq
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 84 7e-17
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 82 3e-16
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 81 7e-16
At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 50 2e-06
At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical t... 49 3e-06
At1g72190.1 68414.m08347 oxidoreductase family protein similar t... 43 2e-04
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 42 4e-04
At3g28790.1 68416.m03593 expressed protein 36 0.019
At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 35 0.058
At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containi... 34 0.076
At4g35240.1 68417.m05009 expressed protein contains Pfam domains... 32 0.41
At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) simil... 30 1.6
At4g15810.1 68417.m02406 chloroplast outer membrane protein, put... 27 8.8
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 27 8.8
At1g44170.2 68414.m05102 aldehyde dehydrogenase, putative (ALDH)... 27 8.8
At1g44170.1 68414.m05101 aldehyde dehydrogenase, putative (ALDH)... 27 8.8
At1g01390.1 68414.m00054 UDP-glucoronosyl/UDP-glucosyl transfera... 27 8.8
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 84.2 bits (199), Expect = 7e-17
Identities = 38/79 (48%), Positives = 54/79 (68%)
Frame = +2
Query: 272 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARXVVPATTAL 451
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E L+ +AR V A ++
Sbjct: 145 RLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASI 204
Query: 452 KAGRWDRALYTGSELAGKT 508
KAG+W+R+ Y G L GKT
Sbjct: 205 KAGKWERSKYVGVSLVGKT 223
Score = 33.1 bits (72), Expect = 0.18
Identities = 18/59 (30%), Positives = 32/59 (54%)
Frame = +3
Query: 93 VLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHXALVVRSATQVTKECWTQA 269
+L+ + +G LL +G + +S E+L ++ AL+VRS T+VT+E + A
Sbjct: 85 ILVTEKLGEAGVNLLREFGDVDCSY-DLSPEDLKKKVAESDALIVRSGTKVTREVFEAA 142
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 82.2 bits (194), Expect = 3e-16
Identities = 39/79 (49%), Positives = 51/79 (64%)
Frame = +2
Query: 272 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARXVVPATTAL 451
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E LM +AR V A ++
Sbjct: 124 RLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASV 183
Query: 452 KAGRWDRALYTGSELAGKT 508
KAG W R Y G L GKT
Sbjct: 184 KAGEWKRNKYVGVSLVGKT 202
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 81.0 bits (191), Expect = 7e-16
Identities = 37/79 (46%), Positives = 52/79 (65%)
Frame = +2
Query: 272 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARXVVPATTAL 451
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E L+ +AR + A ++
Sbjct: 109 RLKVVGRAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASI 168
Query: 452 KAGRWDRALYTGSELAGKT 508
KAG+W R Y G L GKT
Sbjct: 169 KAGKWTRNKYVGVSLVGKT 187
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/55 (30%), Positives = 30/55 (54%)
Frame = +3
Query: 90 SVLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHXALVVRSATQVTKE 254
++L+ + +G +LL Y + +S EEL +I AL+VRS T+V ++
Sbjct: 48 TILVTEKLGQAGIDLLKKYANVDCSY-DLSLEELCTKISLCDALIVRSGTKVGRD 101
>At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent
hydroxypyruvate reductase identical to hydroxypyruvate
reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant
Cell Physiol 1997 Apr;38(4):449-55)
Length = 386
Score = 49.6 bits (113), Expect = 2e-06
Identities = 27/80 (33%), Positives = 40/80 (50%), Gaps = 3/80 (3%)
Frame = +2
Query: 278 KVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARXVVPATTALKA 457
K G +N+DV++A K G+ V N PG + EL +L L AR +V A ++
Sbjct: 88 KAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRG 147
Query: 458 G---RWDRALYTGSELAGKT 508
G W L+ G+ L G+T
Sbjct: 148 GLYEGWLPHLFVGNLLKGQT 167
>At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical to
GI:7677266
Length = 384
Score = 48.8 bits (111), Expect = 3e-06
Identities = 28/80 (35%), Positives = 41/80 (51%), Gaps = 2/80 (2%)
Frame = +2
Query: 275 LKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARXVVPATTALK 454
LK++ AG G D+ID+ +A G+ V G+N +S E +L+L R VP +
Sbjct: 120 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 179
Query: 455 AGRWDRA--LYTGSELAGKT 508
G W+ A Y +L GKT
Sbjct: 180 KGEWNVAGIAYRAYDLEGKT 199
>At1g72190.1 68414.m08347 oxidoreductase family protein similar to
D-3-phosphoglycerate dehydrogenase from Arabidopsis
thaliana [SP|O04130], glyoxylate reductase from Homo
sapiens (gi:6002730); contains Pfam D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain PF02826
Length = 373
Score = 43.2 bits (97), Expect = 2e-04
Identities = 29/97 (29%), Positives = 44/97 (45%), Gaps = 3/97 (3%)
Frame = +2
Query: 227 AFSNSSDQRVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPG---ANALSACELT 397
A + D V+ +K++ + G G+D +D+D+A K G+ V P NA S E+
Sbjct: 100 AMTMQMDSNVISRASNIKLIMQYGVGLDGVDIDAATKHGIKVARIPSEGTGNAASCSEMA 159
Query: 398 CTLMLVLARXVVPATTALKAGRWDRALYTGSELAGKT 508
LML L + +L+ TG L GKT
Sbjct: 160 IYLMLGLLKKQNEMQISLRNRLLGEP--TGDTLLGKT 194
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 41.9 bits (94), Expect = 4e-04
Identities = 22/93 (23%), Positives = 45/93 (48%), Gaps = 1/93 (1%)
Frame = +2
Query: 233 SNSSDQRVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLML 412
S +D +++ L++V G+D ID+ +KG+ V N P +L L+L
Sbjct: 54 SAGADAQLISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLIL 113
Query: 413 VLARXVVPATTALKAGRWDRALY-TGSELAGKT 508
L R + +++G+W + + ++ +GK+
Sbjct: 114 ALLRRLCECDRYVRSGKWKQGEFQLTTKFSGKS 146
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 36.3 bits (80), Expect = 0.019
Identities = 17/40 (42%), Positives = 22/40 (55%)
Frame = -1
Query: 378 SALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPAS 259
S +P TPTP P ST STP P+ PT + TP++
Sbjct: 271 SGASPSGSPTPTPSTPTPSTPTPSTPTPSTPTPSTPTPST 310
Score = 32.7 bits (71), Expect = 0.23
Identities = 17/33 (51%), Positives = 19/33 (57%)
Frame = -1
Query: 351 TPTPFLPAESTSMLSTPAPARPTTFSFTPASNT 253
TPTP P ST STP P+ PT TPA +T
Sbjct: 285 TPTPSTPTPSTPTPSTPTPSTPT--PSTPAPST 315
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = -1
Query: 351 TPTPFLPAESTSMLSTPAPARP 286
TPTP P ST STPAP+ P
Sbjct: 295 TPTPSTPTPSTPTPSTPAPSTP 316
>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
PF05064 Nsp1-like C-terminal region
Length = 739
Score = 34.7 bits (76), Expect = 0.058
Identities = 24/83 (28%), Positives = 39/83 (46%)
Frame = -1
Query: 501 PASSLPVYRARSHLPAFSAVVAGTTXRARTNINVQVSSHALSALAPGALITPTPFLPAES 322
P SS ++ A P+FS VA + + +I S + A TP+ F + S
Sbjct: 241 PGSSSSIFGATGSSPSFS--VASSASGSSPSIFGATGSSPFFGSSSSAGSTPSLFASSSS 298
Query: 321 TSMLSTPAPARPTTFSFTPASNT 253
+ S+P+P +TF+ + SNT
Sbjct: 299 GATTSSPSPFGVSTFNSSSTSNT 321
>At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containing
protein ESTs gb|F20110 and gb|F20109 come from this gene;
contains Pfam profile PF00515: TPR Domain
Length = 1558
Score = 34.3 bits (75), Expect = 0.076
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +2
Query: 92 GFDRRRGWRQVCRTPQR-LRNRHHHQGQDLQGRTSYGDTQPRXSGCAFSNSSDQRVL 259
G R+ RQ +R L N+HH++ QD+Q + Y Q G + S SS +R L
Sbjct: 1165 GAGRKSRQRQPDLMKKRMLLNKHHNRNQDVQQQNIYSPLQKTSKGPSLSKSSPRRAL 1221
>At4g35240.1 68417.m05009 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 828
Score = 31.9 bits (69), Expect = 0.41
Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -3
Query: 544 SRPDPAKAQGWRGL--ASELAASVQSPVPPASLQRSGSWD 431
S P P G+ G AS AA+ + P PP S RS WD
Sbjct: 240 SNPVPGPGPGYYGSSSASTTAAATKPPPPPPSPPRSNGWD 279
>At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) similar
to glutamate decarboxylase (gad) GI:294111 from [Petunia
hybrida]
Length = 494
Score = 29.9 bits (64), Expect = 1.6
Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = +3
Query: 69 KMVVDIKSVLI-VDGVGAKCAELLNAYGIATTTKAKISKEELLMEI 203
++V DI VL +D + +K ++ + GIA K K ++E+LME+
Sbjct: 431 RLVADISKVLHELDTLPSKISKKMGIEGIAENVKEKKMEKEILMEV 476
>At4g15810.1 68417.m02406 chloroplast outer membrane protein,
putative similar to chloroplast protein import component
Toc159 [Pisum sativum] GI:8489806, chloroplast outer
envelope protein 86 [Pisum sativum] GI:599958,
GTP-binding protein [Pisum sativum] GI:576509
Length = 898
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +2
Query: 155 HHHQGQDLQGRTSYGDTQPRXSGCAFSNSSD 247
H+H +L GDT + +G +F NSS+
Sbjct: 491 HNHTFMELDEYEGTGDTSEKLTGSSFQNSSE 521
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/45 (33%), Positives = 28/45 (62%)
Frame = -1
Query: 393 SSHALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPAS 259
SS LS+L+P ++P+P + S++ S+ +P+ P S +P+S
Sbjct: 74 SSSPLSSLSPS--LSPSPPSSSPSSAPPSSLSPSSPPPLSLSPSS 116
>At1g44170.2 68414.m05102 aldehyde dehydrogenase, putative (ALDH)
similar to aldehyde dehydrogenase ALDH [Craterostigma
plantagineum] gi|17065918|emb|CAC84900
Length = 484
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +2
Query: 431 VPATTALKAGRWDRALYTGSELAGK 505
V T+AL +WD+ YTGS G+
Sbjct: 178 VTETSALLEQKWDKIFYTGSSKIGR 202
>At1g44170.1 68414.m05101 aldehyde dehydrogenase, putative (ALDH)
similar to aldehyde dehydrogenase ALDH [Craterostigma
plantagineum] gi|17065918|emb|CAC84900
Length = 484
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +2
Query: 431 VPATTALKAGRWDRALYTGSELAGK 505
V T+AL +WD+ YTGS G+
Sbjct: 178 VTETSALLEQKWDKIFYTGSSKIGR 202
>At1g01390.1 68414.m00054 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 480
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/39 (33%), Positives = 18/39 (46%)
Frame = -1
Query: 369 APGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASNT 253
A G L+ L + + L PAP +PT + P NT
Sbjct: 207 AKGILVNSFVDLESNAIKALQEPAPDKPTVYPIGPLVNT 245
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,217,847
Number of Sequences: 28952
Number of extensions: 281270
Number of successful extensions: 1019
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 950
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1012
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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