BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060600.seq (682 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 122 4e-29 SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 119 4e-28 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 29 0.62 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 27 1.9 SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 27 1.9 SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 27 1.9 SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 27 2.5 SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces pom... 27 2.5 SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.5 SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 3.3 SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 4.4 SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 5.8 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 25 7.7 SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 7.7 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 122 bits (295), Expect = 4e-29 Identities = 58/86 (67%), Positives = 71/86 (82%), Gaps = 2/86 (2%) Frame = +2 Query: 257 EERNLLSVAYKNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYD 430 EERNLLSVAYKN++GARR+SWR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+D Sbjct: 41 EERNLLSVAYKNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHD 100 Query: 431 VLGLLDKHLIPKASNPESKVFYLKMK 508 VL +L+KHLIP A+ ESKVFY KMK Sbjct: 101 VLSVLEKHLIPAATTGESKVFYYKMK 126 Score = 44.0 bits (99), Expect = 2e-05 Identities = 20/47 (42%), Positives = 27/47 (57%) Frame = +1 Query: 511 DYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTSPQHTHL 651 DYYRYLAE GE + S +AY+ A +I+ A++ PT P L Sbjct: 128 DYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGL 174 Score = 39.1 bits (87), Expect = 6e-04 Identities = 21/39 (53%), Positives = 26/39 (66%) Frame = +3 Query: 138 MSVDREELVQRAKLAEQAERYDDMAAAMKEVTETGVELS 254 MS RE V AKLAEQAERY++M MK+V + +LS Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLS 39 >SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 119 bits (286), Expect = 4e-28 Identities = 56/86 (65%), Positives = 71/86 (82%), Gaps = 2/86 (2%) Frame = +2 Query: 257 EERNLLSVAYKNVVGARRSSWRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYD 430 EERNLLSVAYKNV+GARR+SWR++SSIEQK E G+ + ++ KEYR K+E+EL IC D Sbjct: 42 EERNLLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQD 101 Query: 431 VLGLLDKHLIPKASNPESKVFYLKMK 508 +L +L+KHLIP A++ ESKVFY KMK Sbjct: 102 ILTVLEKHLIPNAASAESKVFYYKMK 127 Score = 46.8 bits (106), Expect = 3e-06 Identities = 21/42 (50%), Positives = 27/42 (64%) Frame = +1 Query: 511 DYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTSP 636 DYYRYLAE A GE R + S + Y+ A EI+ A++ PT P Sbjct: 129 DYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHP 170 Score = 40.3 bits (90), Expect = 3e-04 Identities = 20/38 (52%), Positives = 24/38 (63%) Frame = +3 Query: 141 SVDREELVQRAKLAEQAERYDDMAAAMKEVTETGVELS 254 + RE+ V AKLAEQAERY+ M MK V T EL+ Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELT 40 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 29.1 bits (62), Expect = 0.62 Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Frame = +2 Query: 344 KTEGSERKQQMAKEYRVKVEKELREI-CYDVLGLLDKHLIPKASNPESKVFYLKMKEITT 520 K +G RK ++ R E LR C+ + +L + PES+ +YL++KE Sbjct: 1476 KLQGRARKTELHGFIRANAEMVLRSPECF--IKILKDCCVLGHFTPESEHYYLELKESLP 1533 Query: 521 GTLQ 532 G LQ Sbjct: 1534 GVLQ 1537 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 27.5 bits (58), Expect = 1.9 Identities = 18/57 (31%), Positives = 26/57 (45%) Frame = +2 Query: 356 SERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKEITTGT 526 S KQ + KE + +LREIC ++ L K L A V+ E+ TG+ Sbjct: 87 SSVKQSLKKELTIGDLSQLREICPQIIELNYKSLASLALEINKNVYTDLNPELYTGS 143 >SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 27.5 bits (58), Expect = 1.9 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%) Frame = +1 Query: 31 FSAREKLILC-----LNLLVQIHFSPSDKGISELVLFHRPRCPSTGKNWCNVPNWPNKLS 195 FS +E ILC L+L+V + ++ S+ + F +PR K N WPN+ S Sbjct: 39 FSDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETS 98 Query: 196 DM 201 + Sbjct: 99 HL 100 >SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pombe|chr 1|||Manual Length = 986 Score = 27.5 bits (58), Expect = 1.9 Identities = 14/56 (25%), Positives = 23/56 (41%) Frame = +3 Query: 114 ISPLPSSTMSVDREELVQRAKLAEQAERYDDMAAAMKEVTETGVELSTRRGTSFQL 281 I L S D +Q E+Y D AA + ++ +G + R+G+ L Sbjct: 6 IIKLLEQVQSADPNSRIQAELGLRDLEKYHDFAAKLTDIASSGASVPLRQGSLIYL 61 >SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator Prp45|Schizosaccharomyces pombe|chr 3|||Manual Length = 557 Score = 27.1 bits (57), Expect = 2.5 Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 3/71 (4%) Frame = +2 Query: 212 GRDEGSDGNRRRT*HEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSE---RKQQMAK 382 GR+ S G H + SV+ + +R S+ S E + E SE R+Q++ + Sbjct: 341 GRNAASSGPS----HAKPRSTSVSSEERSRSRAGSFSHHSESENEDEDSEAFRRRQELRR 396 Query: 383 EYRVKVEKELR 415 E R + EK+LR Sbjct: 397 ERRRQAEKDLR 407 >SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1185 Score = 27.1 bits (57), Expect = 2.5 Identities = 15/49 (30%), Positives = 23/49 (46%) Frame = +1 Query: 496 PQNERDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTSPQH 642 P N R R + GE RH VED + A + +++ + P +P H Sbjct: 1071 PLNPRTGQREVYFELNGENRHVTVEDKKAAIE---TVTRPRADPGNPGH 1116 >SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 27.1 bits (57), Expect = 2.5 Identities = 25/91 (27%), Positives = 41/91 (45%), Gaps = 9/91 (9%) Frame = +2 Query: 320 RVISSIEQKTEGSERKQQMAKEYRVKVEKE-LREICYDVLGL--LDKHL---IPKASNPE 481 +V+SS + E S + + R E E L E+ +V L +D+ +P+ NP Sbjct: 263 KVLSSSSEDDESSSPEDLLKPIIRSTEEMENLNELEQEVQDLDPIDEGFEEKVPRFRNPS 322 Query: 482 SKVFYLKMKEI---TTGTLQKWPQEKPDILL 565 K FY K+ + + L+ EK D L+ Sbjct: 323 KKAFYEKLHSLRNRSYSKLESLTSEKSDTLI 353 >SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr 2|||Manual Length = 463 Score = 26.6 bits (56), Expect = 3.3 Identities = 12/39 (30%), Positives = 20/39 (51%) Frame = +2 Query: 287 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 403 KN+ S+ R +SS + K +E + M +Y K+E Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388 >SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 26.2 bits (55), Expect = 4.4 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +2 Query: 341 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 466 +K + + KEY K +++LR+ D+ LL KH I + Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369 >SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 25.8 bits (54), Expect = 5.8 Identities = 12/45 (26%), Positives = 22/45 (48%) Frame = +2 Query: 275 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 409 S +Y + G S W+ I ++ K+ G +R ++ Y +KE Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 25.4 bits (53), Expect = 7.7 Identities = 16/46 (34%), Positives = 21/46 (45%) Frame = +1 Query: 43 EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTGKNWCNVPNW 180 EKL+ C HF +G+ + L RC +T CNVP W Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720 >SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosaccharomyces pombe|chr 2|||Manual Length = 421 Score = 25.4 bits (53), Expect = 7.7 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = +2 Query: 359 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 481 E++Q A +YR+KVE+ +I V + L+ L + SNPE Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,833,723 Number of Sequences: 5004 Number of extensions: 57107 Number of successful extensions: 170 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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