BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060599.seq (682 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1A4.01 |apc10|SPBC1E8.06|anaphase-promoting complex subunit ... 84 2e-17 SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces pombe... 28 1.1 SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 26 5.8 SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 7.7 >SPBC1A4.01 |apc10|SPBC1E8.06|anaphase-promoting complex subunit Apc10|Schizosaccharomyces pombe|chr 2|||Manual Length = 189 Score = 84.2 bits (199), Expect = 2e-17 Identities = 35/78 (44%), Positives = 57/78 (73%), Gaps = 1/78 (1%) Frame = +2 Query: 257 LDESYTPSRISIRAGTHFNDLQEIEVIELIEPSGWEMIPIKDI-HDRPIRTYMIQIAVLS 433 LDESYTPS + I AGT F DL+ + +++ EP+GW +P+ D + + ++IQI +L+ Sbjct: 86 LDESYTPSTLRISAGTGFQDLEIVTTVQVEEPTGWVHVPVGDFGRNGLLDVHLIQIKILA 145 Query: 434 NHQNGRDTHMRQIKVHSP 487 NHQ+G+D+H+R IK+++P Sbjct: 146 NHQSGKDSHVRLIKIYAP 163 Score = 67.7 bits (158), Expect = 1e-12 Identities = 32/84 (38%), Positives = 52/84 (61%), Gaps = 6/84 (7%) Frame = +3 Query: 21 MAGLEKDPLVYERSGTVR------EVGNHAIWSLSSCKPGFGIDQLRDDCMETYWQSDGQ 182 MA + ++ L ++S T + ++GN A W+ SS K GF I +RDD ++TYWQSDG Sbjct: 1 MAQIRQEALKKQKSETQKSTEGFVDIGNLAQWTCSSEKSGFPIRLVRDDNIDTYWQSDGS 60 Query: 183 LPHLVNIQFQKKTMVSHIYIYTDY 254 PH ++I+F K+ + ++ +Y Y Sbjct: 61 QPHTIHIKFVKRVSIKYVSMYLQY 84 >SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces pombe|chr 2|||Manual Length = 959 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +2 Query: 311 NDLQEIEVIELIEPSGWEMIPIKDIHD 391 +D +EI+ E+ E S WEMI +D D Sbjct: 692 DDFEEIDTDEIEEQSDWEMISNQDASD 718 >SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 128 Score = 25.8 bits (54), Expect = 5.8 Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 1/54 (1%) Frame = +3 Query: 465 DRSKCIHHVNLPLLIK*V*KFFNCSIPTICHYKII*-FTFACWKECTLGGLSII 623 ++ K IH + L I + F N + +C+ K F+F CW LG + II Sbjct: 25 EKYKIIHRL---LYISIIFLFLNYVVDIVCYVKNYNGFSFFCWVFLNLGVIGII 75 >SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 7.7 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +2 Query: 425 VLSNHQNGRDTHMRQIKVHSPCEPTSFD*ISLEIFQLFNSNNMPL 559 ++ N QN ++ + I + S CEPT FD + L Q S N + Sbjct: 977 IVMNSQNISESSITSILL-SNCEPTGFDRLVLLGNQYLTSGNQDI 1020 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,957,856 Number of Sequences: 5004 Number of extensions: 63367 Number of successful extensions: 134 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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