Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060598.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 106 2e-23
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 102 2e-22
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 102 2e-22
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 102 2e-22
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 101 4e-22
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 106 bits (254), Expect = 2e-23
Identities = 49/87 (56%), Positives = 66/87 (75%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 TGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGV 434
T MV++ GEIT+KANVDY+++VR+T + IG+ + G D C V++ ++QQSP+IA GV
Sbjct: 48 TNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGV 107
Query: 435 HXN--RNDEEVGAGDQGLMFGYATDET 509
H + + EEVGAGDQG MFGYATDET
Sbjct: 108 HGHLTKKPEEVGAGDQGHMFGYATDET 134
Score = 83.8 bits (198), Expect = 1e-16
Identities = 42/70 (60%), Positives = 50/70 (71%), Gaps = 4/70 (5%)
Frame = +1
Query: 124 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKP----VWCFCVAKSHP 291
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TK V+ K++
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63
Query: 292 KLTWIIKKLC 321
I++K C
Sbjct: 64 DYEQIVRKTC 73
Score = 56.8 bits (131), Expect = 1e-08
Identities = 26/42 (61%), Positives = 30/42 (71%)
Frame = +2
Query: 512 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYV 637
E MPLT VLA KL K+ E+R+NG W RPD KTQVT EY+
Sbjct: 136 ELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYI 177
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 102 bits (245), Expect = 2e-22
Identities = 46/87 (52%), Positives = 64/87 (73%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 TGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGV 434
T MV++ GEIT+KA +DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GV
Sbjct: 48 TNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGV 107
Query: 435 HXN--RNDEEVGAGDQGLMFGYATDET 509
H + + E++GAGDQG MFGYATDET
Sbjct: 108 HGHFTKRPEDIGAGDQGHMFGYATDET 134
Score = 84.2 bits (199), Expect = 7e-17
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +1
Query: 124 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITK 255
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TK
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47
Score = 52.4 bits (120), Expect = 3e-07
Identities = 23/41 (56%), Positives = 29/41 (70%)
Frame = +2
Query: 512 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEY 634
E MPL+ VLA K+ ++ E+R+NG W RPD KTQVT EY
Sbjct: 136 ELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEY 176
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 102 bits (245), Expect = 2e-22
Identities = 48/87 (55%), Positives = 64/87 (73%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 TGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGV 434
T MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GV
Sbjct: 48 TNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGV 107
Query: 435 HXN--RNDEEVGAGDQGLMFGYATDET 509
H + + EE+GAGDQG MFGYATDET
Sbjct: 108 HGHFTKCPEEIGAGDQGHMFGYATDET 134
Score = 84.2 bits (199), Expect = 7e-17
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +1
Query: 124 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITK 255
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TK
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47
Score = 53.2 bits (122), Expect = 2e-07
Identities = 24/41 (58%), Positives = 29/41 (70%)
Frame = +2
Query: 512 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEY 634
E MPL+ VLA KL ++ E+R+NG W RPD KTQVT EY
Sbjct: 136 ELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEY 176
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 102 bits (245), Expect = 2e-22
Identities = 48/87 (55%), Positives = 64/87 (73%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 TGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGV 434
T MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GV
Sbjct: 48 TNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGV 107
Query: 435 HXN--RNDEEVGAGDQGLMFGYATDET 509
H + + EE+GAGDQG MFGYATDET
Sbjct: 108 HGHFTKCPEEIGAGDQGHMFGYATDET 134
Score = 84.2 bits (199), Expect = 7e-17
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +1
Query: 124 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITK 255
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TK
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTK 47
Score = 53.2 bits (122), Expect = 2e-07
Identities = 24/41 (58%), Positives = 29/41 (70%)
Frame = +2
Query: 512 ECMPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEY 634
E MPL+ VLA KL ++ E+R+NG W RPD KTQVT EY
Sbjct: 136 ELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEY 176
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 101 bits (242), Expect = 4e-22
Identities = 59/145 (40%), Positives = 82/145 (56%), Gaps = 2/145 (1%)
Frame = +3
Query: 255 TGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGV 434
T MV++ GEIT+ A VDY+K+VR T + IG+ + G D C+V++ ++QQSP+IA GV
Sbjct: 48 TNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGV 107
Query: 435 HXN--RNDEEVGAGDQGLMFGYATDETKNACR*L*CLHTNSIRKLQSSGEMENFGGQDQI 608
H + + E++GAGDQG MFGYATDET L T KL + +
Sbjct: 108 HGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPD 167
Query: 609 QKHRLLANMYLAGGATVPHREFHTV 683
K ++ GGA +P R HTV
Sbjct: 168 GKTQVTVEYKNDGGAMIPIR-VHTV 191
Score = 83.0 bits (196), Expect = 2e-16
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +1
Query: 124 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITK 255
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TK
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTK 47
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,991,453
Number of Sequences: 28952
Number of extensions: 388064
Number of successful extensions: 1045
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1000
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1039
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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