BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060597.seq (681 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g49310.1 68418.m06102 importin alpha-1 subunit, putative simi... 46 2e-05 At4g02150.1 68417.m00287 importin alpha-2 subunit identical to i... 44 9e-05 At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMP... 44 1e-04 At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMP... 44 1e-04 At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMP... 42 3e-04 At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMP... 42 3e-04 At1g02690.2 68414.m00220 importin alpha-2 subunit, putative simi... 42 3e-04 At1g02690.1 68414.m00219 importin alpha-2 subunit, putative simi... 42 3e-04 At3g05720.1 68416.m00640 importin alpha-1 subunit, putative simi... 41 7e-04 At5g52000.1 68418.m06453 importin alpha-1 subunit, putative simi... 38 0.006 At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2) sim... 37 0.014 At1g13780.1 68414.m01617 F-box family protein contains F-box dom... 30 1.6 At1g72470.1 68414.m08380 exocyst subunit EXO70 family protein co... 27 8.7 >At5g49310.1 68418.m06102 importin alpha-1 subunit, putative similar to importin alpha-1 subunit (Karyopherin alpha-1 subunit, KAP alpha) [Arabidopsis thaliana] SWISS-PROT:Q96321 Length = 519 Score = 46.4 bits (105), Expect = 2e-05 Identities = 25/74 (33%), Positives = 42/74 (56%), Gaps = 3/74 (4%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVV---LDDSLIYCRY 277 +KEA WAI+N +GG+P Q+ ++ + + C +L D R I+V L+ LI Sbjct: 384 KKEAIWAISNASVGGSPNQIKYLVEQNCIKALCDILVCPDLRIILVSLGGLEMILIAGEV 443 Query: 278 DENLRKFNSHTLLL 319 D+NLR N ++ ++ Sbjct: 444 DKNLRDVNCYSQMI 457 >At4g02150.1 68417.m00287 importin alpha-2 subunit identical to importin alpha-2 subunit (Karyopherin alpha-2 subunit) (KAP alpha) SP:O04294 from [Arabidopsis thaliana] Length = 531 Score = 44.0 bits (99), Expect = 9e-05 Identities = 20/49 (40%), Positives = 29/49 (59%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEAAW I+N GGT +Q+ M+S + P C LL D + + V L+ Sbjct: 389 KKEAAWGISNATSGGTHDQIKFMVSQGCIKPLCDLLTCPDLKVVTVCLE 437 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/40 (30%), Positives = 23/40 (57%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARD 226 +KEA W I+N+ G +Q+ A++ I+ +LQ+ + Sbjct: 347 KKEACWTISNI-TAGNADQIQAVIDAGIIQSLVWVLQSAE 385 >At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMPA4) similar to importin alpha-1 subunit (Karyopherin alpha-1 subunit, KAP alpha) [Arabidopsis thaliana] SWISS-PROT:Q96321 Length = 538 Score = 43.6 bits (98), Expect = 1e-04 Identities = 20/49 (40%), Positives = 30/49 (61%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEAAWAI+N GG+ EQ+ +++ + P C LL D R + V L+ Sbjct: 393 KKEAAWAISNATSGGSHEQIQYLVTQGCIKPLCDLLICPDPRIVTVCLE 441 Score = 30.3 bits (65), Expect = 1.2 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = +2 Query: 83 THN*LHRCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 T N +KEA W I+N+ G Q++A++ I+ P LLQ Sbjct: 343 TQNHKKSIKKEACWTISNI-TAGNKLQIEAVVGAGIILPLVHLLQ 386 >At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMPA4) similar to importin alpha-1 subunit (Karyopherin alpha-1 subunit, KAP alpha) [Arabidopsis thaliana] SWISS-PROT:Q96321 Length = 538 Score = 43.6 bits (98), Expect = 1e-04 Identities = 20/49 (40%), Positives = 30/49 (61%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEAAWAI+N GG+ EQ+ +++ + P C LL D R + V L+ Sbjct: 393 KKEAAWAISNATSGGSHEQIQYLVTQGCIKPLCDLLICPDPRIVTVCLE 441 Score = 30.3 bits (65), Expect = 1.2 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = +2 Query: 83 THN*LHRCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 T N +KEA W I+N+ G Q++A++ I+ P LLQ Sbjct: 343 TQNHKKSIKKEACWTISNI-TAGNKLQIEAVVGAGIILPLVHLLQ 386 >At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMPA1) similar to importin alpha-1 subunit (Karyopherin alpha-1 subunit, KAP alpha) [Arabidopsis thaliana] SWISS-PROT:Q96321 Length = 532 Score = 42.3 bits (95), Expect = 3e-04 Identities = 20/49 (40%), Positives = 29/49 (59%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEAAWAI+N GG+ +Q+ ++ + P C LL D R I V L+ Sbjct: 386 KKEAAWAISNATSGGSHDQIKYLVEQGCIKPLCDLLVCPDPRIITVCLE 434 Score = 33.5 bits (73), Expect = 0.13 Identities = 15/45 (33%), Positives = 26/45 (57%) Frame = +2 Query: 83 THN*LHRCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 T N +KEA W I+N+ G +Q+ ++ +++SP +LLQ Sbjct: 336 TQNHKKSIKKEACWTISNI-TAGNKDQIQTVVEANLISPLVSLLQ 379 >At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMPA1) similar to importin alpha-1 subunit (Karyopherin alpha-1 subunit, KAP alpha) [Arabidopsis thaliana] SWISS-PROT:Q96321 Length = 532 Score = 42.3 bits (95), Expect = 3e-04 Identities = 20/49 (40%), Positives = 29/49 (59%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEAAWAI+N GG+ +Q+ ++ + P C LL D R I V L+ Sbjct: 386 KKEAAWAISNATSGGSHDQIKYLVEQGCIKPLCDLLVCPDPRIITVCLE 434 Score = 33.5 bits (73), Expect = 0.13 Identities = 15/45 (33%), Positives = 26/45 (57%) Frame = +2 Query: 83 THN*LHRCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 T N +KEA W I+N+ G +Q+ ++ +++SP +LLQ Sbjct: 336 TQNHKKSIKKEACWTISNI-TAGNKDQIQTVVEANLISPLVSLLQ 379 >At1g02690.2 68414.m00220 importin alpha-2 subunit, putative similar to importin alpha-2 subunit (Karyopherin alpha-2 subunit) (KAP alpha) SP:O04294 from [Arabidopsis thaliana] Length = 539 Score = 42.3 bits (95), Expect = 3e-04 Identities = 19/49 (38%), Positives = 28/49 (57%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEA WAI+N GG +Q+ ++S + P C LL D R + V L+ Sbjct: 391 KKEAVWAISNATSGGNHDQIKFLVSQGCIRPLCDLLPCPDPRVVTVTLE 439 Score = 28.7 bits (61), Expect = 3.8 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 +KEA W I+N+ G T Q+ + I+ P LL+ Sbjct: 349 KKEACWTISNITAGNT-SQIQEVFQAGIIRPLINLLE 384 >At1g02690.1 68414.m00219 importin alpha-2 subunit, putative similar to importin alpha-2 subunit (Karyopherin alpha-2 subunit) (KAP alpha) SP:O04294 from [Arabidopsis thaliana] Length = 538 Score = 42.3 bits (95), Expect = 3e-04 Identities = 19/49 (38%), Positives = 28/49 (57%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEA WAI+N GG +Q+ ++S + P C LL D R + V L+ Sbjct: 390 KKEAVWAISNATSGGNHDQIKFLVSQGCIRPLCDLLPCPDPRVVTVTLE 438 Score = 28.7 bits (61), Expect = 3.8 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 +KEA W I+N+ G T Q+ + I+ P LL+ Sbjct: 348 KKEACWTISNITAGNT-SQIQEVFQAGIIRPLINLLE 383 >At3g05720.1 68416.m00640 importin alpha-1 subunit, putative similar to importin alpha subunit (Karyopherin alpha subunit) (KAP alpha) SP:O22478 from [Lycopersicon esculentum] Length = 528 Score = 41.1 bits (92), Expect = 7e-04 Identities = 18/49 (36%), Positives = 30/49 (61%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLD 253 +KEAAWAI N GG+ +Q+ ++ + + P C LL D + ++V L+ Sbjct: 374 KKEAAWAICNAIAGGSYKQIMFLVKQECIKPLCDLLTCSDTQLVMVCLE 422 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 +KEA W ++N+ G Q+ A+ DI LLQ Sbjct: 332 RKEACWTVSNI-TAGCQSQIQAVFDADICPALVNLLQ 367 >At5g52000.1 68418.m06453 importin alpha-1 subunit, putative similar to importin alpha-1 subunit (Karyopherin alpha-1 subunit, KAP alpha) [Arabidopsis thaliana] SWISS-PROT:Q96321 Length = 441 Score = 37.9 bits (84), Expect = 0.006 Identities = 18/52 (34%), Positives = 27/52 (51%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQARDHRAIIVVLDDSL 262 +KEA WAI+N+ L G+ +Q+ M + C +L D R I+ D L Sbjct: 314 KKEAVWAISNMALNGSHDQIKYMAEQSCIKQLCDILVYSDERTTILKCLDGL 365 >At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2) similar to importin alpha 2 [Capsicum annuum] GI:13752562; contains Pfam profiles PF01749: Importin beta binding domain, PF00514: Armadillo/beta-catenin-like repeat; non-consensus GG donor splice site at exon 1 and 6; CT acceptor splice site at exon 2 Length = 444 Score = 36.7 bits (81), Expect = 0.014 Identities = 17/41 (41%), Positives = 28/41 (68%), Gaps = 4/41 (9%) Frame = +2 Query: 107 QKEAAWAITNLCLGGTPEQLDAMLSCDILSP----YCALLQ 217 +KEAAWAI+N GG+P+Q+ M+ +++ +CAL+Q Sbjct: 391 KKEAAWAISNATSGGSPDQIKYMVEQGVVNHCVIFWCALIQ 431 Score = 33.1 bits (72), Expect = 0.17 Identities = 16/45 (35%), Positives = 24/45 (53%) Frame = +2 Query: 83 THN*LHRCQKEAAWAITNLCLGGTPEQLDAMLSCDILSPYCALLQ 217 THN +KEA W I+N+ G +Q+ A+ ++ P LLQ Sbjct: 341 THNHKKSIKKEACWTISNI-TAGNRDQIQAVCEAGLICPLVNLLQ 384 >At1g13780.1 68414.m01617 F-box family protein contains F-box domain Pfam:PF00646 Length = 451 Score = 29.9 bits (64), Expect = 1.6 Identities = 16/52 (30%), Positives = 28/52 (53%) Frame = -3 Query: 274 PAIDE*VIQHYDDGSVVPGLEQGAVRRQNITGQHGVQLFRGASKAQVCDGPG 119 P ++E + H DD VV + +++R +I +H + LFR ++ D PG Sbjct: 190 PVLEELIYLH-DDKLVVTRVRSRSLKRFSIPFRHKLSLFRRVTQTFEIDAPG 240 >At1g72470.1 68414.m08380 exocyst subunit EXO70 family protein contains Pfam domain PF03081: Exo70 exocyst complex subunit; Length = 633 Score = 27.5 bits (58), Expect = 8.7 Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 4/59 (6%) Frame = -2 Query: 296 IFLNFHHTCNR*VSHPALR*WLGGPW----PGAGRSTATEYHRTAWRPVVQGCLQGTGL 132 I N H+ + P LR +G + G R AT Y R AW V+ CL+ GL Sbjct: 467 IMNNAHYIVQKIKGSPELREMIGDLYLRKLTGKFRQAATYYQRAAWVKVLY-CLRDEGL 524 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,336,636 Number of Sequences: 28952 Number of extensions: 288631 Number of successful extensions: 593 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 554 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 591 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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