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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060593.seq
         (685 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0)              66   3e-11
SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.)              50   2e-06
SB_22212| Best HMM Match : AmoA (HMM E-Value=0.6)                      29   2.7  

>SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0)
          Length = 358

 Score = 65.7 bits (153), Expect = 3e-11
 Identities = 29/48 (60%), Positives = 35/48 (72%)
 Frame = +3

Query: 246 EVNEIIXETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQ 389
           ++ EII   H NVKYLP   +P N+ A PDVVE  KDAD+L+FVVPHQ
Sbjct: 53  KLTEIINTKHMNVKYLPDFLIPENIHANPDVVECVKDADILVFVVPHQ 100



 Score = 52.4 bits (120), Expect = 3e-07
 Identities = 22/49 (44%), Positives = 36/49 (73%), Gaps = 1/49 (2%)
 Frame = +1

Query: 100 KQPKNKVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 243
           ++ + +V I+GSGNWGS IA+IVG+N    ++ F + V M+ YEE+++G
Sbjct: 3   EEGRKRVAIIGSGNWGSVIARIVGQNVKEHNDVFFEGVEMYTYEELVDG 51



 Score = 34.3 bits (75), Expect = 0.093
 Identities = 16/23 (69%), Positives = 18/23 (78%)
 Frame = +1

Query: 535 AVLMGANIASEVAEEKFCETTIG 603
           +VLMGAN+A EVA E F E TIG
Sbjct: 126 SVLMGANLAKEVARELFGEATIG 148


>SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 772

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 21/44 (47%), Positives = 32/44 (72%), Gaps = 1/44 (2%)
 Frame = +1

Query: 115 KVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 243
           KV ++GSGNWG+AIA+I+G N     + F ++V M+VY+ +I G
Sbjct: 229 KVTVLGSGNWGTAIARIIGDNVRKKPHLFHNKVQMYVYDSLING 272



 Score = 40.3 bits (90), Expect = 0.001
 Identities = 27/76 (35%), Positives = 37/76 (48%)
 Frame = +3

Query: 243 KEVNEIIXETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLG 422
           ++++EII   HENVK LPG K+P NV                       F+ ++C  +  
Sbjct: 273 RKLSEIINTEHENVKDLPGFKIPPNV-----------------------FLDSVCQKIKS 309

Query: 423 KIKPTAAALSLIKGFD 470
            IKP   A+SLIKG D
Sbjct: 310 SIKPDVLAISLIKGLD 325



 Score = 35.5 bits (78), Expect = 0.040
 Identities = 17/41 (41%), Positives = 26/41 (63%)
 Frame = +1

Query: 535 AVLMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDY 657
           +V+MGAN+A EVA+  F ETTIG R      + ++++   Y
Sbjct: 348 SVMMGANLADEVAKGFFSETTIGSRLEEHGYIFKELLNQPY 388


>SB_22212| Best HMM Match : AmoA (HMM E-Value=0.6)
          Length = 772

 Score = 29.5 bits (63), Expect = 2.7
 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
 Frame = +3

Query: 285 KYLPGHKLPSNVVAVPDVVEAAKDA-DLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461
           + L G+  P   +  P+VV+ +  A  L+I VVP     T    L G   P AA +   +
Sbjct: 523 RQLNGNDAPMAALVGPEVVDVSVGAASLMIRVVPKFSTATFTRQLNGNDAPIAALVGP-E 581

Query: 462 GFDIAEGWASILYHIFYK 515
             D++ G AS++  +  K
Sbjct: 582 VVDVSVGAASLMIRVVPK 599



 Score = 28.7 bits (61), Expect = 4.6
 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
 Frame = +3

Query: 285 KYLPGHKLPSNVVAVPDVVEAAKDAD-LLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461
           + L G+  P   +  P+VV+ +  A  L+I VVP     T    L G   P AA +   +
Sbjct: 481 RQLNGNDAPMAALVGPEVVDVSVGAAALMIRVVPKFSTATFTRQLNGNDAPMAALVGP-E 539

Query: 462 GFDIAEGWASILYHIFYK 515
             D++ G AS++  +  K
Sbjct: 540 VVDVSVGAASLMIRVVPK 557



 Score = 27.9 bits (59), Expect = 8.1
 Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
 Frame = +3

Query: 285 KYLPGHKLPSNVVAVPDVVEAAKD-ADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461
           + L G+  P   +  P+VV+ +   A L+I VVP     T    L G   P AA +   +
Sbjct: 116 RQLNGNDAPIAALVGPEVVDVSVGAASLMIRVVPKFSTATFTRQLNGNDAPMAALVG-PE 174

Query: 462 GFDIAEGWASILYHIFYK 515
             D++ G A+++  +  K
Sbjct: 175 VVDVSVGAAALMIRVVPK 192



 Score = 27.9 bits (59), Expect = 8.1
 Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
 Frame = +3

Query: 285 KYLPGHKLPSNVVAVPDVVEAAKD-ADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461
           + L G+  P   +  P+VV+ +   A L+I VVP     T    L G   P AA +   +
Sbjct: 565 RQLNGNDAPIAALVGPEVVDVSVGAASLMIRVVPKFSTATFTRQLNGNDAPIAALVG-PE 623

Query: 462 GFDIAEGWASILYHIFYK 515
             D++ G A+++  +  K
Sbjct: 624 VVDVSVGAAALMIRVVPK 641


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,760,249
Number of Sequences: 59808
Number of extensions: 348850
Number of successful extensions: 821
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 775
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 821
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1769412099
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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