BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060593.seq (685 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0) 66 3e-11 SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06 SB_22212| Best HMM Match : AmoA (HMM E-Value=0.6) 29 2.7 >SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0) Length = 358 Score = 65.7 bits (153), Expect = 3e-11 Identities = 29/48 (60%), Positives = 35/48 (72%) Frame = +3 Query: 246 EVNEIIXETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQ 389 ++ EII H NVKYLP +P N+ A PDVVE KDAD+L+FVVPHQ Sbjct: 53 KLTEIINTKHMNVKYLPDFLIPENIHANPDVVECVKDADILVFVVPHQ 100 Score = 52.4 bits (120), Expect = 3e-07 Identities = 22/49 (44%), Positives = 36/49 (73%), Gaps = 1/49 (2%) Frame = +1 Query: 100 KQPKNKVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 243 ++ + +V I+GSGNWGS IA+IVG+N ++ F + V M+ YEE+++G Sbjct: 3 EEGRKRVAIIGSGNWGSVIARIVGQNVKEHNDVFFEGVEMYTYEELVDG 51 Score = 34.3 bits (75), Expect = 0.093 Identities = 16/23 (69%), Positives = 18/23 (78%) Frame = +1 Query: 535 AVLMGANIASEVAEEKFCETTIG 603 +VLMGAN+A EVA E F E TIG Sbjct: 126 SVLMGANLAKEVARELFGEATIG 148 >SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 772 Score = 49.6 bits (113), Expect = 2e-06 Identities = 21/44 (47%), Positives = 32/44 (72%), Gaps = 1/44 (2%) Frame = +1 Query: 115 KVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 243 KV ++GSGNWG+AIA+I+G N + F ++V M+VY+ +I G Sbjct: 229 KVTVLGSGNWGTAIARIIGDNVRKKPHLFHNKVQMYVYDSLING 272 Score = 40.3 bits (90), Expect = 0.001 Identities = 27/76 (35%), Positives = 37/76 (48%) Frame = +3 Query: 243 KEVNEIIXETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLG 422 ++++EII HENVK LPG K+P NV F+ ++C + Sbjct: 273 RKLSEIINTEHENVKDLPGFKIPPNV-----------------------FLDSVCQKIKS 309 Query: 423 KIKPTAAALSLIKGFD 470 IKP A+SLIKG D Sbjct: 310 SIKPDVLAISLIKGLD 325 Score = 35.5 bits (78), Expect = 0.040 Identities = 17/41 (41%), Positives = 26/41 (63%) Frame = +1 Query: 535 AVLMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDY 657 +V+MGAN+A EVA+ F ETTIG R + ++++ Y Sbjct: 348 SVMMGANLADEVAKGFFSETTIGSRLEEHGYIFKELLNQPY 388 >SB_22212| Best HMM Match : AmoA (HMM E-Value=0.6) Length = 772 Score = 29.5 bits (63), Expect = 2.7 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 1/78 (1%) Frame = +3 Query: 285 KYLPGHKLPSNVVAVPDVVEAAKDA-DLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461 + L G+ P + P+VV+ + A L+I VVP T L G P AA + + Sbjct: 523 RQLNGNDAPMAALVGPEVVDVSVGAASLMIRVVPKFSTATFTRQLNGNDAPIAALVGP-E 581 Query: 462 GFDIAEGWASILYHIFYK 515 D++ G AS++ + K Sbjct: 582 VVDVSVGAASLMIRVVPK 599 Score = 28.7 bits (61), Expect = 4.6 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 1/78 (1%) Frame = +3 Query: 285 KYLPGHKLPSNVVAVPDVVEAAKDAD-LLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461 + L G+ P + P+VV+ + A L+I VVP T L G P AA + + Sbjct: 481 RQLNGNDAPMAALVGPEVVDVSVGAAALMIRVVPKFSTATFTRQLNGNDAPMAALVGP-E 539 Query: 462 GFDIAEGWASILYHIFYK 515 D++ G AS++ + K Sbjct: 540 VVDVSVGAASLMIRVVPK 557 Score = 27.9 bits (59), Expect = 8.1 Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 1/78 (1%) Frame = +3 Query: 285 KYLPGHKLPSNVVAVPDVVEAAKD-ADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461 + L G+ P + P+VV+ + A L+I VVP T L G P AA + + Sbjct: 116 RQLNGNDAPIAALVGPEVVDVSVGAASLMIRVVPKFSTATFTRQLNGNDAPMAALVG-PE 174 Query: 462 GFDIAEGWASILYHIFYK 515 D++ G A+++ + K Sbjct: 175 VVDVSVGAAALMIRVVPK 192 Score = 27.9 bits (59), Expect = 8.1 Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 1/78 (1%) Frame = +3 Query: 285 KYLPGHKLPSNVVAVPDVVEAAKD-ADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 461 + L G+ P + P+VV+ + A L+I VVP T L G P AA + + Sbjct: 565 RQLNGNDAPIAALVGPEVVDVSVGAASLMIRVVPKFSTATFTRQLNGNDAPIAALVG-PE 623 Query: 462 GFDIAEGWASILYHIFYK 515 D++ G A+++ + K Sbjct: 624 VVDVSVGAAALMIRVVPK 641 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,760,249 Number of Sequences: 59808 Number of extensions: 348850 Number of successful extensions: 821 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 775 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 821 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1769412099 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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