Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060589.seq
(684 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 229 2e-62
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 229 2e-62
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 229 2e-62
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 222 3e-60
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 37 2e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase... 24 1.6
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.7
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 22 6.3
AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cycl... 22 6.3
AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellif... 21 8.3
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 229 bits (560), Expect = 2e-62
Identities = 110/143 (76%), Positives = 118/143 (82%)
Frame = +1
Query: 256 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 435
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 436 REHALLAFTLGVKQLIVGVNKMDSMNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVAFVPI 615
REHALLAFTLGVKQLIVGVNKMDS + +++ +++GYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 616 SGWHGDNMLELQPKCLGSXGWQV 684
SGWHGDNMLE+ K GW V
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTV 143
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 229 bits (560), Expect = 2e-62
Identities = 110/143 (76%), Positives = 118/143 (82%)
Frame = +1
Query: 256 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 435
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 17 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 76
Query: 436 REHALLAFTLGVKQLIVGVNKMDSMNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVAFVPI 615
REHALLAFTLGVKQLIVGVNKMDS + +++ +++GYNPAAVAFVPI
Sbjct: 77 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 136
Query: 616 SGWHGDNMLELQPKCLGSXGWQV 684
SGWHGDNMLE+ K GW V
Sbjct: 137 SGWHGDNMLEVSSKMPWFKGWTV 159
Score = 32.7 bits (71), Expect = 0.003
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +2
Query: 209 WVLDKLKAERELGIT 253
WVLDKLKAERE GIT
Sbjct: 1 WVLDKLKAERERGIT 15
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 229 bits (560), Expect = 2e-62
Identities = 110/143 (76%), Positives = 118/143 (82%)
Frame = +1
Query: 256 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 435
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 74 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 133
Query: 436 REHALLAFTLGVKQLIVGVNKMDSMNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVAFVPI 615
REHALLAFTLGVKQLIVGVNKMDS + +++ +++GYNPAAVAFVPI
Sbjct: 134 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 193
Query: 616 SGWHGDNMLELQPKCLGSXGWQV 684
SGWHGDNMLE+ K GW V
Sbjct: 194 SGWHGDNMLEVSSKMPWFKGWTV 216
Score = 151 bits (365), Expect = 8e-39
Identities = 70/72 (97%), Positives = 70/72 (97%)
Frame = +2
Query: 38 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 217
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 218 DKLKAERELGIT 253
DKLKAERE GIT
Sbjct: 61 DKLKAERERGIT 72
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 222 bits (542), Expect = 3e-60
Identities = 106/143 (74%), Positives = 117/143 (81%)
Frame = +1
Query: 256 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 435
DIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQT
Sbjct: 74 DIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQT 133
Query: 436 REHALLAFTLGVKQLIVGVNKMDSMNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVAFVPI 615
REHALLAFTLGVKQLIVGVNKMD + + +++ +++GYN A+VAFVPI
Sbjct: 134 REHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPI 193
Query: 616 SGWHGDNMLELQPKCLGSXGWQV 684
SGWHGDNMLE PK GW+V
Sbjct: 194 SGWHGDNMLEPSPKTPWYKGWKV 216
Score = 151 bits (365), Expect = 8e-39
Identities = 70/72 (97%), Positives = 70/72 (97%)
Frame = +2
Query: 38 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 217
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 218 DKLKAERELGIT 253
DKLKAERE GIT
Sbjct: 61 DKLKAERERGIT 72
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.1 bits (82), Expect = 2e-04
Identities = 37/118 (31%), Positives = 50/118 (42%)
Frame = +1
Query: 295 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 474
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 475 QLIVGVNKMDSMNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVAFVPISGWHGDNMLEL 648
+IV +NK+D N + ++ K+ LG + V IS G N+ EL
Sbjct: 247 PIIVAINKIDKPNIDIIK--VQYELAKHGIVIEELG---GEIQCVKISALKGINLREL 299
Score = 25.4 bits (53), Expect = 0.51
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 50 KTHINIVVIGHVDSGKST 103
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 59 INIVVIGHVDSGKST 103
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase
protein.
Length = 1143
Score = 23.8 bits (49), Expect = 1.6
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = -3
Query: 628 RAIQKWARKRQQLGCSPIFLMYEDTSFLISSNLGS 524
+A +KWA + C L +DT ++ +LGS
Sbjct: 652 QAFRKWAADTFAVACETFCLDDDDTLLEVALSLGS 686
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 2.7
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 51 FSLPIFG*SRITNCV*Y 1
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 280 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 381
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 280 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 381
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 21.8 bits (44), Expect = 6.3
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -1
Query: 657 FWLKLQHVVSVPSRNGHESDSSWV 586
F L L H++ V +N + + WV
Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58
>AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cyclase
beta-3 protein.
Length = 832
Score = 21.8 bits (44), Expect = 6.3
Identities = 6/14 (42%), Positives = 11/14 (78%)
Frame = +3
Query: 615 FWMARRQHVGASTK 656
+W+ +R+H +STK
Sbjct: 593 YWLEKREHRSSSTK 606
>AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellifera
ORF for hypotheticalprotein. ).
Length = 998
Score = 21.4 bits (43), Expect = 8.3
Identities = 10/46 (21%), Positives = 22/46 (47%)
Frame = -1
Query: 261 NIVVIPSSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIPPHL 124
N VV+ + + + +N K P P ++ ++V S+P ++
Sbjct: 613 NCVVVEETIPSLNSTNVTLSTKCPYPSYYSYIGVLTLVATSMPTYI 658
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 197,568
Number of Sequences: 438
Number of extensions: 4023
Number of successful extensions: 27
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20830365
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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