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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060585.seq
         (686 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   142   6e-35
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   141   7e-35
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c...    26   4.4  
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra...    26   5.9  
SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosacch...    26   5.9  

>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  142 bits (343), Expect = 6e-35
 Identities = 74/139 (53%), Positives = 82/139 (58%)
 Frame = +3

Query: 255 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXX 434
           AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH     
Sbjct: 58  AGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQ 117

Query: 435 XXXXXXXXXXXXXXXXXXXXQARGQLLKRFPELPLVVADKVQEINKTKQGCHLS*GASKA 614
                                ARG  ++  PE+PLVV D VQ   KTK+   L     KA
Sbjct: 118 NEKRYAIASAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALL-KEIKA 176

Query: 615 XSDILXVYQVSAVFRAGXG 671
             D++ V   S   RAG G
Sbjct: 177 YRDVIKVAN-SRKLRAGKG 194



 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 32/58 (55%), Positives = 42/58 (72%)
 Frame = +1

Query: 82  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 255
           ++ ARP VS+Y+ K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++
Sbjct: 1   MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57


>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  141 bits (342), Expect = 7e-35
 Identities = 74/139 (53%), Positives = 82/139 (58%)
 Frame = +3

Query: 255 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXX 434
           AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH     
Sbjct: 58  AGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQ 117

Query: 435 XXXXXXXXXXXXXXXXXXXXQARGQLLKRFPELPLVVADKVQEINKTKQGCHLS*GASKA 614
                                ARG  ++  PE+PLVV D VQ   KTK+   L     KA
Sbjct: 118 NEKRYAISSAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALL-KEIKA 176

Query: 615 XSDILXVYQVSAVFRAGXG 671
             D++ V   S   RAG G
Sbjct: 177 YRDVVKVAN-SRKLRAGKG 194



 Score = 70.5 bits (165), Expect = 2e-13
 Identities = 33/58 (56%), Positives = 42/58 (72%)
 Frame = +1

Query: 82  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 255
           ++ ARP VS+YS K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++
Sbjct: 1   MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57


>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 606

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = -1

Query: 143 APCTVSLFSEYTDTKGRATDRLISLL 66
           A C V ++SEYT   G ++  L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515


>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
           3-phosphatidyltransferase Pis1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 251

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%)
 Frame = -2

Query: 100 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 23
           K+G LP+ SF Y +  +PL Y TL+AV+
Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213


>SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 593

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 14/48 (29%), Positives = 22/48 (45%)
 Frame = -1

Query: 548 GYNQGKLGKSFQ*LSSSLNERWDAGSSKRCRQGRSPLSEVDATVPTPP 405
           G+ + K GK    + +    R+D   SKR     +    V A +P+PP
Sbjct: 216 GFPENKHGKFHFHIPNERRSRFDQPPSKRMALTSTARESVPAPLPSPP 263


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,575,499
Number of Sequences: 5004
Number of extensions: 49434
Number of successful extensions: 128
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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