BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060576.seq (694 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619 C... 94 9e-20 At1g26830.1 68414.m03270 cullin, putative similar to Cullin homo... 58 6e-09 At1g69670.1 68414.m08018 cullin, putative contains similarity to... 57 1e-08 At4g02570.1 68417.m00351 cullin family protein similar to cullin... 36 0.019 At1g59800.1 68414.m06733 cullin-related similar to cullin 3 [Hom... 34 0.078 At1g59790.1 68414.m06732 cullin-related low similarity to Hs-CUL... 32 0.31 At1g30620.1 68414.m03745 UDP-D-xylose 4-epimerase, putative (MUR... 25 2.7 At4g07380.1 68417.m01133 hypothetical protein 29 2.9 At2g40630.1 68415.m05011 expressed protein 29 3.9 At5g26150.1 68418.m03110 protein kinase family protein contains ... 28 5.1 At5g44480.1 68418.m05450 NAD-dependent epimerase/dehydratase fam... 25 7.7 At2g34850.1 68415.m04279 NAD-dependent epimerase/dehydratase fam... 25 8.1 At5g37010.1 68418.m04438 expressed protein 27 8.9 At3g03540.1 68416.m00355 phosphoesterase family protein similar ... 27 8.9 At1g54170.1 68414.m06175 ataxin-2-related similar to SCA2 (GI:17... 27 8.9 >At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619 Cullin homolog 4A (CUL-4A) {Homo sapiens}; contains Pfam profile PF00888: Cullin family Length = 792 Score = 93.9 bits (223), Expect = 9e-20 Identities = 43/102 (42%), Positives = 62/102 (60%) Frame = +3 Query: 252 KSIAYSLEELYQAVENMCSHKMASQLYVNLTNLVEAHVKANIEQFLSESMDRQVFLKRMD 431 K I++ LE LYQAV+N+C HK+ +LY + E H+ A ++ + ++ D VFL R++ Sbjct: 110 KKISFDLESLYQAVDNLCLHKLDGKLYDQIEKECEEHISAALQSLVGQNTDLTVFLSRVE 169 Query: 432 DCWRAHCRQMIMIRSIFLYLDRTYVLKTLVYIQFGTWGLDLF 557 CW+ C QM+MIRSI L LDR YV++ GL LF Sbjct: 170 KCWQDFCDQMLMIRSIALTLDRKYVIQNPNVRSLWEMGLQLF 211 Score = 45.2 bits (102), Expect = 4e-05 Identities = 24/64 (37%), Positives = 35/64 (54%), Gaps = 1/64 (1%) Frame = +1 Query: 55 EKISNFSLV-NPNSNGIKMSSTSMNKPGVTAKKLIIKNFKSKPNLPDNYQETTWSKLREA 231 +K+ F + +P +S P T KK +IK K+KP LP N++E TW KL+ A Sbjct: 44 DKVVGFHMEEDPTPAAANLSRKKATLPQPT-KKFVIKLNKAKPTLPTNFEENTWEKLQSA 102 Query: 232 VVAI 243 + AI Sbjct: 103 IRAI 106 >At1g26830.1 68414.m03270 cullin, putative similar to Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from [Homo sapiens]; contains Pfam profile PF00888: Cullin family Length = 732 Score = 58.0 bits (134), Expect = 6e-09 Identities = 29/83 (34%), Positives = 44/83 (53%) Frame = +3 Query: 267 SLEELYQAVENMCSHKMASQLYVNLTNLVEAHVKANIEQFLSESMDRQVFLKRMDDCWRA 446 S EELY+ NM HK +LY + +H+K + L E+ FL+ ++ W Sbjct: 48 SFEELYRNAYNMVLHKFGEKLYTGFIATMTSHLKEKSK--LIEAAQGGSFLEELNKKWNE 105 Query: 447 HCRQMIMIRSIFLYLDRTYVLKT 515 H + + MIR I +Y+DRTY+ T Sbjct: 106 HNKALEMIRDILMYMDRTYIEST 128 >At1g69670.1 68414.m08018 cullin, putative contains similarity to Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from [Homo sapiens]; contains Pfam profile PF00888: Cullin family Length = 732 Score = 56.8 bits (131), Expect = 1e-08 Identities = 30/83 (36%), Positives = 42/83 (50%) Frame = +3 Query: 267 SLEELYQAVENMCSHKMASQLYVNLTNLVEAHVKANIEQFLSESMDRQVFLKRMDDCWRA 446 S EELY+ NM HK +LY L + H+K + E FL+ ++ W Sbjct: 48 SFEELYRNAYNMVLHKYGDKLYTGLVTTMTFHLKEICKSI--EEAQGGAFLELLNRKWND 105 Query: 447 HCRQMIMIRSIFLYLDRTYVLKT 515 H + + MIR I +Y+DRTYV T Sbjct: 106 HNKALQMIRDILMYMDRTYVSTT 128 >At4g02570.1 68417.m00351 cullin family protein similar to cullin 3 [Homo sapiens] GI:3639052; contains Pfam profile PF00888: Cullin family Length = 738 Score = 36.3 bits (80), Expect = 0.019 Identities = 21/82 (25%), Positives = 36/82 (43%), Gaps = 4/82 (4%) Frame = +3 Query: 279 LYQAVENMCS----HKMASQLYVNLTNLVEAHVKANIEQFLSESMDRQVFLKRMDDCWRA 446 LY + NMC+ H + QLY E ++ + + L E D + L+ + W Sbjct: 42 LYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHD-EFMLRELFKRWSN 100 Query: 447 HCRQMIMIRSIFLYLDRTYVLK 512 H + + F YLDR ++ + Sbjct: 101 HKVMVRWLSRFFYYLDRYFIAR 122 >At1g59800.1 68414.m06733 cullin-related similar to cullin 3 [Homo sapiens] GI:3639052 Length = 255 Score = 34.3 bits (75), Expect = 0.078 Identities = 19/78 (24%), Positives = 36/78 (46%) Frame = +3 Query: 279 LYQAVENMCSHKMASQLYVNLTNLVEAHVKANIEQFLSESMDRQVFLKRMDDCWRAHCRQ 458 ++ A +C++K QLY L+E + + L E D + L+ + W AH Sbjct: 43 MHTATYRICAYKNPQQLYDKYRELIENYAIQTVLPSLREKHD-ECMLRELAKRWNAHKLL 101 Query: 459 MIMIRSIFLYLDRTYVLK 512 + + +YLD +++ K Sbjct: 102 VRLFSRRLVYLDDSFLSK 119 >At1g59790.1 68414.m06732 cullin-related low similarity to Hs-CUL-1 [Homo sapiens] GI:1381142 Length = 374 Score = 32.3 bits (70), Expect = 0.31 Identities = 20/86 (23%), Positives = 39/86 (45%), Gaps = 2/86 (2%) Frame = +3 Query: 276 ELYQAVENMCSHKM--ASQLYVNLTNLVEAHVKANIEQFLSESMDRQVFLKRMDDCWRAH 449 +LY + +MC + + QLY ++E + + L E D + L+ + W H Sbjct: 47 KLYTIIYDMCVQRSDYSQQLYEKYRKVIEDYTIQTVLPSLREKHDEDM-LRELVKRWNNH 105 Query: 450 CRQMIMIRSIFLYLDRTYVLKTLVYI 527 + + F+Y+DR V ++ + I Sbjct: 106 KIMVKWLSKFFVYIDRHLVRRSKIPI 131 >At1g30620.1 68414.m03745 UDP-D-xylose 4-epimerase, putative (MUR4) similar to SP|P55180 UDP-glucose 4-epimerase (EC 5.1.3.2) from Bacillus subtilis, GI:3021357 UDP-galactose 4-epimerase from Cyamopsis tetragonoloba; contains Pfam profile PF01370 NAD dependent epimerase/dehydratase family; contains TIGRfam profile TIGR01179: UDP-glucose 4-epimerase Length = 419 Score = 25.4 bits (53), Expect(2) = 2.7 Identities = 9/14 (64%), Positives = 14/14 (100%) Frame = +3 Query: 330 YVNLTNLVEAHVKA 371 Y+++T+LV+AHVKA Sbjct: 306 YIDVTDLVDAHVKA 319 Score = 22.2 bits (45), Expect(2) = 2.7 Identities = 8/19 (42%), Positives = 11/19 (57%) Frame = +3 Query: 183 PSRQLSGNDMEQAAGSCCR 239 P Q+ G D + A G+C R Sbjct: 286 PGLQIKGTDYKTADGTCVR 304 >At4g07380.1 68417.m01133 hypothetical protein Length = 168 Score = 29.1 bits (62), Expect = 2.9 Identities = 16/62 (25%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Frame = +1 Query: 4 HEESCSKRVKINEMSAE-EKISNFSLVNPNSNGIKMSSTSMNKPGVTAKKLIIKNFKSKP 180 +EE + V +NE + E +K+ + N NG ++ + + + AKK ++KN P Sbjct: 91 NEEVSQEEVMVNEETKEVDKMEEETQTNGEDNGTEVINPEIADVELPAKKRVVKNKAGLP 150 Query: 181 NL 186 + Sbjct: 151 GV 152 >At2g40630.1 68415.m05011 expressed protein Length = 535 Score = 28.7 bits (61), Expect = 3.9 Identities = 15/50 (30%), Positives = 29/50 (58%) Frame = +1 Query: 7 EESCSKRVKINEMSAEEKISNFSLVNPNSNGIKMSSTSMNKPGVTAKKLI 156 +E SKRVK+ +++++ K S+ + NSN ++ + S K +K L+ Sbjct: 35 DELGSKRVKVPDLASDAKTSSLQ-SHGNSNSVQQPNLSSEKLSKVSKVLV 83 >At5g26150.1 68418.m03110 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 703 Score = 28.3 bits (60), Expect = 5.1 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +3 Query: 222 AGSCCRHSDLKSIAYSLEELYQAVENMCSHK 314 A S H+D++ YS+EE+ +A E +H+ Sbjct: 396 AVSALAHNDVRYRKYSIEEIEEATERFANHR 426 >At5g44480.1 68418.m05450 NAD-dependent epimerase/dehydratase family protein similar to SP|P55180 UDP-glucose 4-epimerase (EC 5.1.3.2) from Bacillus subtilis, GI:3021357 UDP-galactose 4-epimerase from Cyamopsis tetragonoloba; contains Pfam profile PF01370 NAD dependent epimerase/dehydratase family Length = 436 Score = 25.4 bits (53), Expect(2) = 7.7 Identities = 9/14 (64%), Positives = 14/14 (100%) Frame = +3 Query: 330 YVNLTNLVEAHVKA 371 Y+++T+LV+AHVKA Sbjct: 330 YIDVTDLVDAHVKA 343 Score = 20.6 bits (41), Expect(2) = 7.7 Identities = 7/19 (36%), Positives = 11/19 (57%) Frame = +3 Query: 183 PSRQLSGNDMEQAAGSCCR 239 P Q+ G D + + G+C R Sbjct: 310 PGLQVKGTDYKTSDGTCIR 328 >At2g34850.1 68415.m04279 NAD-dependent epimerase/dehydratase family protein similar to UDP-galactose 4-epimerase from Cyamopsis tetragonoloba GI:3021357, Lactococcus lactis GI:3703056; contains Pfam profile PF01370 NAD dependent epimerase/dehydratase family Length = 236 Score = 25.4 bits (53), Expect(2) = 8.1 Identities = 9/14 (64%), Positives = 14/14 (100%) Frame = +3 Query: 330 YVNLTNLVEAHVKA 371 Y+++T+LV+AHVKA Sbjct: 124 YIDVTDLVDAHVKA 137 Score = 20.6 bits (41), Expect(2) = 8.1 Identities = 7/19 (36%), Positives = 10/19 (52%) Frame = +3 Query: 183 PSRQLSGNDMEQAAGSCCR 239 P Q+ G D + G+C R Sbjct: 104 PGLQIKGTDYKTVDGTCVR 122 >At5g37010.1 68418.m04438 expressed protein Length = 637 Score = 27.5 bits (58), Expect = 8.9 Identities = 10/21 (47%), Positives = 17/21 (80%) Frame = +1 Query: 73 SLVNPNSNGIKMSSTSMNKPG 135 S +NPNS+G ++S++ N+PG Sbjct: 222 SEINPNSSGNSVNSSNNNRPG 242 >At3g03540.1 68416.m00355 phosphoesterase family protein similar to SP|P95246 Phospholipase C 2 precursor (EC 3.1.4.3) {Mycobacterium tuberculosis}; contains Pfam profile PF04185: Phosphoesterase family Length = 521 Score = 27.5 bits (58), Expect = 8.9 Identities = 10/13 (76%), Positives = 11/13 (84%) Frame = +1 Query: 631 RERGGDSVDIIFC 669 RERGGD DI+FC Sbjct: 482 RERGGDENDIVFC 494 >At1g54170.1 68414.m06175 ataxin-2-related similar to SCA2 (GI:1770390) [Homo sapiens]; similar to ataxin-2 (GI:3005020) [Mus musculus] Length = 587 Score = 27.5 bits (58), Expect = 8.9 Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = +1 Query: 37 NEMSAEEKISNFSLVNPNSNGIKMSSTSMNKPGVTAKKL--IIKNFKSKPN 183 +E SA SN +++P+S+G S S P KL K+FK P+ Sbjct: 439 SENSAASSASNLPILSPSSSGSLSSEKSTLNPNAKEFKLNPNAKSFKPSPS 489 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,871,913 Number of Sequences: 28952 Number of extensions: 273860 Number of successful extensions: 749 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 729 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 746 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1477286152 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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