BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060574.seq (686 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family pro... 31 0.95 At4g33810.1 68417.m04798 glycosyl hydrolase family 10 protein si... 28 6.7 At5g28650.1 68418.m03508 WRKY family transcription factor DNA-bi... 27 8.8 >At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family protein low similarity to ubiquitin-conjugating BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831, ubiquitin-conjugating enzyme [Mus musculus] GI:3319990; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 907 Score = 30.7 bits (66), Expect = 0.95 Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 1/55 (1%) Frame = +3 Query: 231 VSAIPGVSNDAWDLGDDEIISGIXDTKIS-KSISETAALNVINSHRNSTKNVNRS 392 ++ +P VS+ +W LGD I+ G I+ + AA N+ H+ K NR+ Sbjct: 232 LTLLPCVSHASWQLGDWCILPGSSHCDIAERQTPNVAAYNLNECHKTFQKGFNRN 286 >At4g33810.1 68417.m04798 glycosyl hydrolase family 10 protein similar to tapetum specific protein GI:3885492 from [Zea mays] Length = 536 Score = 27.9 bits (59), Expect = 6.7 Identities = 8/13 (61%), Positives = 12/13 (92%) Frame = -3 Query: 222 GHNLVWDDPLVRP 184 GH ++WDDPL++P Sbjct: 248 GHTVLWDDPLMQP 260 >At5g28650.1 68418.m03508 WRKY family transcription factor DNA-binding protein WRKY3, parsley, PIR:S72445 Length = 330 Score = 27.5 bits (58), Expect = 8.8 Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 4/47 (8%) Frame = -1 Query: 647 LTGIPRPAESV*SSSRLTNCANRVSAGSAPI*THCSP----RVKRAL 519 L G+P+ ++ + SR T+C+ + GS HCS RVKR++ Sbjct: 203 LIGLPQGSDHISQHSRRTSCSGSLKCGSKSK-CHCSKKRKLRVKRSI 248 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,688,532 Number of Sequences: 28952 Number of extensions: 231646 Number of successful extensions: 611 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 590 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 611 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1457719448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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