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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060574.seq
         (686 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family pro...    31   0.95 
At4g33810.1 68417.m04798 glycosyl hydrolase family 10 protein si...    28   6.7  
At5g28650.1 68418.m03508 WRKY family transcription factor DNA-bi...    27   8.8  

>At2g33770.1 68415.m04141 ubiquitin-conjugating enzyme family
           protein low similarity to ubiquitin-conjugating
           BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831,
           ubiquitin-conjugating enzyme [Mus musculus] GI:3319990;
           contains Pfam profile PF00179: Ubiquitin-conjugating
           enzyme
          Length = 907

 Score = 30.7 bits (66), Expect = 0.95
 Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
 Frame = +3

Query: 231 VSAIPGVSNDAWDLGDDEIISGIXDTKIS-KSISETAALNVINSHRNSTKNVNRS 392
           ++ +P VS+ +W LGD  I+ G     I+ +     AA N+   H+   K  NR+
Sbjct: 232 LTLLPCVSHASWQLGDWCILPGSSHCDIAERQTPNVAAYNLNECHKTFQKGFNRN 286


>At4g33810.1 68417.m04798 glycosyl hydrolase family 10 protein
           similar to tapetum specific protein GI:3885492 from [Zea
           mays]
          Length = 536

 Score = 27.9 bits (59), Expect = 6.7
 Identities = 8/13 (61%), Positives = 12/13 (92%)
 Frame = -3

Query: 222 GHNLVWDDPLVRP 184
           GH ++WDDPL++P
Sbjct: 248 GHTVLWDDPLMQP 260


>At5g28650.1 68418.m03508 WRKY family transcription factor
           DNA-binding protein WRKY3, parsley, PIR:S72445
          Length = 330

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 4/47 (8%)
 Frame = -1

Query: 647 LTGIPRPAESV*SSSRLTNCANRVSAGSAPI*THCSP----RVKRAL 519
           L G+P+ ++ +   SR T+C+  +  GS     HCS     RVKR++
Sbjct: 203 LIGLPQGSDHISQHSRRTSCSGSLKCGSKSK-CHCSKKRKLRVKRSI 248


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,688,532
Number of Sequences: 28952
Number of extensions: 231646
Number of successful extensions: 611
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 590
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 611
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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