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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060568.seq
         (622 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g25880.1 68418.m03071 malate oxidoreductase, putative similar...    55   3e-08
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar...    55   3e-08
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar...    54   1e-07
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar...    49   2e-06
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar...    46   2e-05
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar...    46   2e-05
At2g26960.1 68415.m03234 myb family transcription factor (MYB81)...    31   0.62 
At2g29360.1 68415.m03567 tropinone reductase, putative / tropine...    31   0.81 
At5g04340.1 68418.m00426 zinc finger (C2H2 type) family protein ...    28   5.7  
At2g29370.1 68415.m03568 tropinone reductase, putative / tropine...    27   7.6  

>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP:P12628) {Phaseolus vulgaris}
          Length = 588

 Score = 55.2 bits (127), Expect = 3e-08
 Identities = 22/36 (61%), Positives = 30/36 (83%)
 Frame = +1

Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621
           E+LF++LL +NVE+ LP+VY P VG ACQ++G IYR
Sbjct: 117 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 152



 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 29/70 (41%), Positives = 41/70 (58%)
 Frame = +2

Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478
           +V  G   +RDPR NKGLAFT +ER A  I GLL     SQ+ Q      ++ +Y   L 
Sbjct: 45  SVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPLQ 104

Query: 479 KYLYLVELQD 508
           +Y+ L++LQ+
Sbjct: 105 RYMALMDLQE 114


>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP:P51615) {Vitis vinifera}
          Length = 581

 Score = 55.2 bits (127), Expect = 3e-08
 Identities = 23/36 (63%), Positives = 30/36 (83%)
 Frame = +1

Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621
           E+LF++LL +NVE+ LPIVY P VG ACQ+FG I+R
Sbjct: 110 ERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFR 145



 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 29/70 (41%), Positives = 39/70 (55%)
 Frame = +2

Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478
           +V  G   LRDPR NKGLAFT +ER    + GLL      Q+ Q      ++ +Y+  L 
Sbjct: 38  SVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQ 97

Query: 479 KYLYLVELQD 508
           KY+ L ELQ+
Sbjct: 98  KYMALTELQE 107


>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP|P12628) {Phaseolus vulgaris}
          Length = 588

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 21/36 (58%), Positives = 30/36 (83%)
 Frame = +1

Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621
           E+LF++LL +NVE+ LP+VY P VG ACQ++G I+R
Sbjct: 117 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFR 152



 Score = 48.8 bits (111), Expect = 3e-06
 Identities = 28/70 (40%), Positives = 41/70 (58%)
 Frame = +2

Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478
           +V  G   +RDPR NKGLAFT +ER A  + GLL     SQ+ Q      ++ +Y   L 
Sbjct: 45  SVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPLQ 104

Query: 479 KYLYLVELQD 508
           +Y+ L++LQ+
Sbjct: 105 RYMALMDLQE 114


>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
           malate oxidoreductase (NADP-dependent malic enzyme)
           GB:P34105 (Populus balsamifera subsp. trichocarpa)
          Length = 646

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 18/35 (51%), Positives = 29/35 (82%)
 Frame = +1

Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIY 618
           E+LF++LL ++VE+ LP++Y P VG ACQ++G I+
Sbjct: 175 ERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIF 209



 Score = 48.8 bits (111), Expect = 3e-06
 Identities = 27/70 (38%), Positives = 41/70 (58%)
 Frame = +2

Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478
           +V  G   LRDP  NKGLAF+  ER A  + GLL     SQ+ Q+     ++ +Y+  L 
Sbjct: 103 SVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQ 162

Query: 479 KYLYLVELQD 508
           KY+ +++LQ+
Sbjct: 163 KYMAMMDLQE 172


>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
           NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
           precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
           tuberosum}
          Length = 607

 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 16/36 (44%), Positives = 26/36 (72%)
 Frame = +1

Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621
           E L++R+L +N++ + PI+Y P VGL CQ +  +YR
Sbjct: 117 ETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYR 152



 Score = 38.7 bits (86), Expect = 0.003
 Identities = 20/43 (46%), Positives = 23/43 (53%)
 Frame = +2

Query: 308 RGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLD 436
           RG D L DP  NK   F L ER  LGI GLL  +  +  +Q D
Sbjct: 38  RGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVMTCVQQCD 80


>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
           NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
           precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
           tuberosum}
          Length = 623

 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 16/36 (44%), Positives = 26/36 (72%)
 Frame = +1

Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621
           E +++++L  N+E+Y PIVY P VGL CQ +  ++R
Sbjct: 124 ETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159



 Score = 43.2 bits (97), Expect = 1e-04
 Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 2/60 (3%)
 Frame = +2

Query: 296 PNVV--RGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYED 469
           P +V  +G+D L DP  NKG AFT+ ER  L + GLL       E+Q+      + R E+
Sbjct: 39  PTIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEE 98


>At2g26960.1 68415.m03234 myb family transcription factor (MYB81)
           contains PFAM profile: myb DNA binding domain PF00249;
           identical to cDNA  putative transcription factor (MYB81)
           mRNA, partial cds GI:3941513
          Length = 427

 Score = 31.1 bits (67), Expect = 0.62
 Identities = 16/43 (37%), Positives = 24/43 (55%)
 Frame = +2

Query: 305 VRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQL 433
           +R ++HLR P L KG     EE+  + +H LL  K+    E+L
Sbjct: 63  LRWVNHLR-PDLKKGAFTEKEEKRVIELHALLGNKWARMAEEL 104


>At2g29360.1 68415.m03567 tropinone reductase, putative / tropine
           dehydrogenase, putative similar to tropinone reductase
           SP:P50165 from [Datura stramonium]
          Length = 271

 Score = 30.7 bits (66), Expect = 0.81
 Identities = 16/45 (35%), Positives = 22/45 (48%)
 Frame = -1

Query: 313 ASDNIRANDIAGYFVVIPTFIEEGSRIEFFVRMYARALFGXIAFV 179
           ASDNIR N +  +F+  P   E  S  EF   + +R   G +  V
Sbjct: 187 ASDNIRTNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEV 231


>At5g04340.1 68418.m00426 zinc finger (C2H2 type) family protein
           contains Pfam domain, PF00096: Zinc finger, C2H2 type
          Length = 238

 Score = 27.9 bits (59), Expect = 5.7
 Identities = 25/81 (30%), Positives = 38/81 (46%)
 Frame = +2

Query: 314 IDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLNKYLYL 493
           ++ L  PRL+  +  TL +  ALG HG    + K    + D   ++ D Y   +   L L
Sbjct: 3   LETLTSPRLSSPMP-TLFQDSALGFHGSKGKRSKRSRSEFDRQSLTEDEY---IALCLML 58

Query: 494 VELQDEXRNCSLDFWPRTSRS 556
           +  +D  RN  LD  P +S S
Sbjct: 59  L-ARDGDRNRDLDL-PSSSSS 77


>At2g29370.1 68415.m03568 tropinone reductase, putative / tropine
           dehydrogenase, putative similar to SP|P50162 Tropinone
           reductase-I (EC 1.1.1.206) (TR-I) (Tropine
           dehydrogenase) {Datura stramonium}
          Length = 268

 Score = 27.5 bits (58), Expect = 7.6
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = -1

Query: 313 ASDNIRANDIAGYFVVIPT 257
           ASDNIRAN +  +F+  P+
Sbjct: 187 ASDNIRANSVCPWFITTPS 205


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,207,730
Number of Sequences: 28952
Number of extensions: 229767
Number of successful extensions: 542
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 542
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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