BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060568.seq (622 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 55 3e-08 At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 55 3e-08 At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 54 1e-07 At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 49 2e-06 At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 46 2e-05 At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 46 2e-05 At2g26960.1 68415.m03234 myb family transcription factor (MYB81)... 31 0.62 At2g29360.1 68415.m03567 tropinone reductase, putative / tropine... 31 0.81 At5g04340.1 68418.m00426 zinc finger (C2H2 type) family protein ... 28 5.7 At2g29370.1 68415.m03568 tropinone reductase, putative / tropine... 27 7.6 >At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME) (SP:P12628) {Phaseolus vulgaris} Length = 588 Score = 55.2 bits (127), Expect = 3e-08 Identities = 22/36 (61%), Positives = 30/36 (83%) Frame = +1 Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621 E+LF++LL +NVE+ LP+VY P VG ACQ++G IYR Sbjct: 117 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 152 Score = 49.2 bits (112), Expect = 2e-06 Identities = 29/70 (41%), Positives = 41/70 (58%) Frame = +2 Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478 +V G +RDPR NKGLAFT +ER A I GLL SQ+ Q ++ +Y L Sbjct: 45 SVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPLQ 104 Query: 479 KYLYLVELQD 508 +Y+ L++LQ+ Sbjct: 105 RYMALMDLQE 114 >At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME) (SP:P51615) {Vitis vinifera} Length = 581 Score = 55.2 bits (127), Expect = 3e-08 Identities = 23/36 (63%), Positives = 30/36 (83%) Frame = +1 Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621 E+LF++LL +NVE+ LPIVY P VG ACQ+FG I+R Sbjct: 110 ERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFR 145 Score = 50.0 bits (114), Expect = 1e-06 Identities = 29/70 (41%), Positives = 39/70 (55%) Frame = +2 Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478 +V G LRDPR NKGLAFT +ER + GLL Q+ Q ++ +Y+ L Sbjct: 38 SVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQ 97 Query: 479 KYLYLVELQD 508 KY+ L ELQ+ Sbjct: 98 KYMALTELQE 107 >At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME) (SP|P12628) {Phaseolus vulgaris} Length = 588 Score = 53.6 bits (123), Expect = 1e-07 Identities = 21/36 (58%), Positives = 30/36 (83%) Frame = +1 Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621 E+LF++LL +NVE+ LP+VY P VG ACQ++G I+R Sbjct: 117 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFR 152 Score = 48.8 bits (111), Expect = 3e-06 Identities = 28/70 (40%), Positives = 41/70 (58%) Frame = +2 Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478 +V G +RDPR NKGLAFT +ER A + GLL SQ+ Q ++ +Y L Sbjct: 45 SVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPLQ 104 Query: 479 KYLYLVELQD 508 +Y+ L++LQ+ Sbjct: 105 RYMALMDLQE 114 >At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to malate oxidoreductase (NADP-dependent malic enzyme) GB:P34105 (Populus balsamifera subsp. trichocarpa) Length = 646 Score = 49.2 bits (112), Expect = 2e-06 Identities = 18/35 (51%), Positives = 29/35 (82%) Frame = +1 Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIY 618 E+LF++LL ++VE+ LP++Y P VG ACQ++G I+ Sbjct: 175 ERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIF 209 Score = 48.8 bits (111), Expect = 3e-06 Identities = 27/70 (38%), Positives = 41/70 (58%) Frame = +2 Query: 299 NVVRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLN 478 +V G LRDP NKGLAF+ ER A + GLL SQ+ Q+ ++ +Y+ L Sbjct: 103 SVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQ 162 Query: 479 KYLYLVELQD 508 KY+ +++LQ+ Sbjct: 163 KYMAMMDLQE 172 >At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to NAD-dependent malic enzyme 59 kDa isoform, mitochondrial precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum tuberosum} Length = 607 Score = 46.0 bits (104), Expect = 2e-05 Identities = 16/36 (44%), Positives = 26/36 (72%) Frame = +1 Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621 E L++R+L +N++ + PI+Y P VGL CQ + +YR Sbjct: 117 ETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYR 152 Score = 38.7 bits (86), Expect = 0.003 Identities = 20/43 (46%), Positives = 23/43 (53%) Frame = +2 Query: 308 RGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLD 436 RG D L DP NK F L ER LGI GLL + + +Q D Sbjct: 38 RGADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVMTCVQQCD 80 >At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to NAD-dependent malic enzyme 62 kDa isoform, mitochondrial precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum tuberosum} Length = 623 Score = 46.0 bits (104), Expect = 2e-05 Identities = 16/36 (44%), Positives = 26/36 (72%) Frame = +1 Query: 514 EKLFFRLLAENVEKYLPIVYXPNVGLACQRFGLIYR 621 E +++++L N+E+Y PIVY P VGL CQ + ++R Sbjct: 124 ETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159 Score = 43.2 bits (97), Expect = 1e-04 Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 2/60 (3%) Frame = +2 Query: 296 PNVV--RGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYED 469 P +V +G+D L DP NKG AFT+ ER L + GLL E+Q+ + R E+ Sbjct: 39 PTIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEE 98 >At2g26960.1 68415.m03234 myb family transcription factor (MYB81) contains PFAM profile: myb DNA binding domain PF00249; identical to cDNA putative transcription factor (MYB81) mRNA, partial cds GI:3941513 Length = 427 Score = 31.1 bits (67), Expect = 0.62 Identities = 16/43 (37%), Positives = 24/43 (55%) Frame = +2 Query: 305 VRGIDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQL 433 +R ++HLR P L KG EE+ + +H LL K+ E+L Sbjct: 63 LRWVNHLR-PDLKKGAFTEKEEKRVIELHALLGNKWARMAEEL 104 >At2g29360.1 68415.m03567 tropinone reductase, putative / tropine dehydrogenase, putative similar to tropinone reductase SP:P50165 from [Datura stramonium] Length = 271 Score = 30.7 bits (66), Expect = 0.81 Identities = 16/45 (35%), Positives = 22/45 (48%) Frame = -1 Query: 313 ASDNIRANDIAGYFVVIPTFIEEGSRIEFFVRMYARALFGXIAFV 179 ASDNIR N + +F+ P E S EF + +R G + V Sbjct: 187 ASDNIRTNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEV 231 >At5g04340.1 68418.m00426 zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type Length = 238 Score = 27.9 bits (59), Expect = 5.7 Identities = 25/81 (30%), Positives = 38/81 (46%) Frame = +2 Query: 314 IDHLRDPRLNKGLAFTLEERXALGIHGLLAAKFKSQEEQLDICRISVDRYEDNLNKYLYL 493 ++ L PRL+ + TL + ALG HG + K + D ++ D Y + L L Sbjct: 3 LETLTSPRLSSPMP-TLFQDSALGFHGSKGKRSKRSRSEFDRQSLTEDEY---IALCLML 58 Query: 494 VELQDEXRNCSLDFWPRTSRS 556 + +D RN LD P +S S Sbjct: 59 L-ARDGDRNRDLDL-PSSSSS 77 >At2g29370.1 68415.m03568 tropinone reductase, putative / tropine dehydrogenase, putative similar to SP|P50162 Tropinone reductase-I (EC 1.1.1.206) (TR-I) (Tropine dehydrogenase) {Datura stramonium} Length = 268 Score = 27.5 bits (58), Expect = 7.6 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = -1 Query: 313 ASDNIRANDIAGYFVVIPT 257 ASDNIRAN + +F+ P+ Sbjct: 187 ASDNIRANSVCPWFITTPS 205 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,207,730 Number of Sequences: 28952 Number of extensions: 229767 Number of successful extensions: 542 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 529 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 542 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1255974912 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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