BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060564.seq (685 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 87 8e-18 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 84 1e-16 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 79 2e-15 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.8 At5g41460.1 68418.m05035 fringe-related protein strong similarit... 28 6.6 At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 6.6 At1g80300.1 68414.m09401 chloroplast ADP, ATP carrier protein 1 ... 28 6.6 At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 6.6 At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 8.8 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 87.4 bits (207), Expect = 8e-18 Identities = 36/72 (50%), Positives = 52/72 (72%) Frame = +2 Query: 101 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHVKFTWLGLI 280 E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGH K ++ + Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 281 SSMVKSMKISVP 316 K ++ VP Sbjct: 64 IFTSKKLEDIVP 75 Score = 83.4 bits (197), Expect = 1e-16 Identities = 35/57 (61%), Positives = 42/57 (73%) Frame = +1 Query: 256 KVHLVGXDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLRE 426 K H V DIF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++ Sbjct: 56 KCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKD 112 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 83.8 bits (198), Expect = 1e-16 Identities = 35/72 (48%), Positives = 51/72 (70%) Frame = +2 Query: 101 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHVKFTWLGLI 280 ++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGH K ++ + Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 281 SSMVKSMKISVP 316 K ++ VP Sbjct: 64 IFTSKKLEDIVP 75 Score = 81.4 bits (192), Expect = 5e-16 Identities = 34/57 (59%), Positives = 42/57 (73%) Frame = +1 Query: 256 KVHLVGXDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLRE 426 K H V DIF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++ Sbjct: 56 KCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKD 112 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 79.4 bits (187), Expect = 2e-15 Identities = 33/72 (45%), Positives = 48/72 (66%) Frame = +2 Query: 101 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHVKFTWLGLI 280 ++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGH K ++ + Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 281 SSMVKSMKISVP 316 K ++ VP Sbjct: 64 IFTAKKLEDIVP 75 Score = 77.8 bits (183), Expect = 6e-15 Identities = 32/57 (56%), Positives = 42/57 (73%) Frame = +1 Query: 256 KVHLVGXDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLRE 426 K H V DIF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++ Sbjct: 56 KCHFVAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKD 112 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 3.8 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -2 Query: 201 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 94 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 27.9 bits (59), Expect = 6.6 Identities = 11/26 (42%), Positives = 14/26 (53%) Frame = +3 Query: 75 FKTQQWVTSKTHTSRPETPGPQPPSP 152 F T Q K++ S P +P P PP P Sbjct: 83 FHTNQTAVIKSYASPPPSPPPPPPPP 108 >At3g53330.1 68416.m05884 plastocyanin-like domain-containing protein similar to mavicyanin SP:P80728 from [Cucurbita pepo] Length = 310 Score = 27.9 bits (59), Expect = 6.6 Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%) Frame = +3 Query: 99 SKTHT-SRPETPGPQPPS 149 SKTH SRP TP P PPS Sbjct: 127 SKTHERSRPITPSPPPPS 144 >At1g80300.1 68414.m09401 chloroplast ADP, ATP carrier protein 1 / ADP, ATP translocase 1 / adenine nucleotide translocase 1 (AATP1) identical to SP|Q39002 Chloroplast ADP,ATP carrier protein 1, chloroplast precursor (ADP/ATP translocase 1) (Adenine nucleotide translocase 1) {Arabidopsis thaliana} Length = 624 Score = 27.9 bits (59), Expect = 6.6 Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +1 Query: 244 ARPRKVHLVGXDIFNG-KKYEDICPSTHNMDVPHVKR 351 A+PR +H + FNG KK++ P+ H + + H +R Sbjct: 37 AKPRNLHGLSLS-FNGHKKFQTFEPTLHGISISHKER 72 >At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein contains INTERPRO domain, IPR001878: Zn-finger CCHC type Length = 353 Score = 27.9 bits (59), Expect = 6.6 Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%) Frame = +3 Query: 102 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 227 KT T P PP PCN PC S + + R CP Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66 >At1g20860.1 68414.m02613 phosphate transporter family protein similar to phosphate transporter [Catharanthus roseus] GI:2208908, inorganic phosphate transporter 1 [Solanum tuberosum] GI:1420871; contains Pfam profile PF00083: major facilitator superfamily protein Length = 534 Score = 27.5 bits (58), Expect = 8.8 Identities = 17/45 (37%), Positives = 20/45 (44%) Frame = +3 Query: 78 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 212 K Q V S++H S T P PP P P K S C +H R Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,380,471 Number of Sequences: 28952 Number of extensions: 280574 Number of successful extensions: 817 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 740 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 806 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1447936096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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