SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060561.seq
         (686 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC29A3.18 |cyt1||cytochrome c1|Schizosaccharomyces pombe|chr 2...   131   1e-31
SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce...    29   0.63 
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p...    29   0.63 
SPBC2F12.14c |gua1||IMP dehydrogenase Gua1 |Schizosaccharomyces ...    28   1.5  
SPBC1539.01c |||mitochondrial ribosomal protein subunit L15|Schi...    27   2.5  
SPCC1235.06 |sif1||Sad1 interacting factor 1|Schizosaccharomyces...    27   3.4  
SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch...    27   3.4  
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    27   3.4  
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch...    26   5.9  
SPCC61.01c |str2|str1, SPCC622.20c|siderophore-iron transporter ...    25   7.8  

>SPBC29A3.18 |cyt1||cytochrome c1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 307

 Score =  131 bits (316), Expect = 1e-31
 Identities = 57/83 (68%), Positives = 70/83 (84%)
 Frame = +2

Query: 260 DHASVRRGYEVYKQVCKACHSLQYIAFRNLVNVTHTEDEAKAEAAEVMIKDGPDEEGNYF 439
           DHAS+RRG++VY++VC ACHSL  IA+R+LV VTHT DEAK  A+EV  +DGPD+EGN F
Sbjct: 87  DHASLRRGFQVYREVCSACHSLNLIAWRHLVGVTHTADEAKQMASEVEYEDGPDDEGNMF 146

Query: 440 ERPGKLSDYLPSPYPNENAARAA 508
           +RPGKLSD+LP PYPN  AARA+
Sbjct: 147 KRPGKLSDFLPPPYPNVEAARAS 169



 Score = 85.4 bits (202), Expect = 7e-18
 Identities = 36/65 (55%), Positives = 46/65 (70%)
 Frame = +1

Query: 481 PQ*KCGSCCNNGAYPPDLSLICSGRKGGEDYIFALLTGYMEPPAGVVLREGQNYNPYXPG 660
           P  +     NNGA PPDLS +  GR GG+DYI++LLTGY EPPAGV + +G N+NP+ PG
Sbjct: 161 PNVEAARASNNGAAPPDLSCVVRGRHGGQDYIYSLLTGYTEPPAGVEVPDGMNFNPFFPG 220

Query: 661 GAISM 675
             I+M
Sbjct: 221 TQIAM 225


>SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 29.1 bits (62), Expect = 0.63
 Identities = 21/62 (33%), Positives = 32/62 (51%)
 Frame = -2

Query: 652 SMDCSSVPHVALHQQEVPCNQSVARRCNLRRPYVPSRSETSPVGKHHCCSTSRIFIGVWR 473
           S D +SV   +LH++ +  N S  R+ + R+PY    SE S + K H    +R+ IG   
Sbjct: 103 SFDHASV--TSLHRRSLSSNTSTPRKYHSRKPY----SEPSVIDKEHL--QNRVSIGTTH 154

Query: 472 RQ 467
            Q
Sbjct: 155 TQ 156


>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1323

 Score = 29.1 bits (62), Expect = 0.63
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -3

Query: 159 QHLHLPLPKWTATLFCFLSILECWWTADKPEA 64
           +H ++PLPK  A +FC    L C++T    E+
Sbjct: 822 KHQNIPLPKTCAAIFCGNDELVCFFTIKADES 853


>SPBC2F12.14c |gua1||IMP dehydrogenase Gua1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 524

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = +1

Query: 583 LLTGYMEPPAGVVLREGQNYNPYXPGGAIS 672
           LL G  E P    +REGQ Y  Y   G+I+
Sbjct: 395 LLAGTTESPGEYYVREGQRYKSYRGMGSIA 424


>SPBC1539.01c |||mitochondrial ribosomal protein subunit
           L15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 210

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 12/17 (70%), Positives = 15/17 (88%)
 Frame = +2

Query: 35  TSFEKIWSSLASGLSAV 85
           +SFEKI+SS+ SGL AV
Sbjct: 159 SSFEKIYSSIVSGLVAV 175


>SPCC1235.06 |sif1||Sad1 interacting factor 1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 257

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 12/34 (35%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
 Frame = +1

Query: 553 RKGGEDY-IFALLTGYMEPPAGVVLREGQNYNPY 651
           R  G  Y + A L   + PP G ++R   +Y PY
Sbjct: 197 RNNGPSYSLLASLASQLPPPYGAMIRHTTSYVPY 230


>SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1316

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = +2

Query: 188 CAGIRL*SSPTSQPLESQWLVQPLDHASVRR 280
           C G+ +    TS+P  + W++QPL    + R
Sbjct: 112 CVGVLIRDPLTSRPSHTLWVLQPLKPTRITR 142


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 18/56 (32%), Positives = 25/56 (44%)
 Frame = -1

Query: 674 IEMAPPGKYGL*FCPSRSTTPAGGSM*PVSSAKM*SSPPLRPEQIRDKSGG*APLL 507
           I + P G+      P RS    G    P+SS++  S+PP  P  I  +S    P L
Sbjct: 351 IPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPL 406


>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 9/19 (47%), Positives = 14/19 (73%)
 Frame = -2

Query: 610 QEVPCNQSVARRCNLRRPY 554
           +E+PC Q V R C+L++ Y
Sbjct: 103 EEMPCEQLVRRVCDLKQGY 121


>SPCC61.01c |str2|str1, SPCC622.20c|siderophore-iron transporter
           Str2 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 597

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 22/87 (25%), Positives = 35/87 (40%)
 Frame = +2

Query: 116 KSVAVHFGSGRWRCWSSTFCT*AVCAGIRL*SSPTSQPLESQWLVQPLDHASVRRGYEVY 295
           +SV+ H   G       TF +     G  +     S+ L S+ L    DH  V     ++
Sbjct: 471 QSVSSHAKLGMNLTLYLTFSSVGGAFGSAIAGGVWSKRLSSRLLHDLKDHLPVPEIESIF 530

Query: 296 KQVCKACHSLQYIAFRNLVNVTHTEDE 376
           + +  A    Q    RN++NV +T  E
Sbjct: 531 RDLRTALSYPQGTQIRNIINVAYTATE 557


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,035,035
Number of Sequences: 5004
Number of extensions: 61498
Number of successful extensions: 200
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -