Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060559.seq
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 169 4e-43
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 168 6e-43
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 168 6e-43
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 92 6e-20
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 92 8e-20
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 75 9e-15
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.007
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.007
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 30 0.27
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 30 0.35
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 27 3.3
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 26 5.7
SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 5.7
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 10.0
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 10.0
SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 25 10.0
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 25 10.0
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 169 bits (410), Expect = 4e-43
Identities = 84/126 (66%), Positives = 93/126 (73%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET KY T+I APGHRDFIKNMITGTSQADCAVLI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISK 129
Query: 438 NGQTREHALLAFXLGVKQLXRRSNKMDSTEPPYSEPXFEXXXXXXXXXXXXXGYNPAAVA 617
+GQTREHALLA+ LGVKQL NKMD+T +S+ FE G+NP V
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVP 187
Query: 618 FVAISG 635
FV +SG
Sbjct: 188 FVPVSG 193
Score = 140 bits (338), Expect = 2e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 168 bits (409), Expect = 6e-43
Identities = 83/126 (65%), Positives = 93/126 (73%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET KY T+I APGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 438 NGQTREHALLAFXLGVKQLXRRSNKMDSTEPPYSEPXFEXXXXXXXXXXXXXGYNPAAVA 617
+GQTREHALLA+ LGVKQL NKMD+T +S+ FE G+NP V
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVP 187
Query: 618 FVAISG 635
FV +SG
Sbjct: 188 FVPVSG 193
Score = 140 bits (338), Expect = 2e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 168 bits (409), Expect = 6e-43
Identities = 83/126 (65%), Positives = 93/126 (73%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET KY T+I APGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 438 NGQTREHALLAFXLGVKQLXRRSNKMDSTEPPYSEPXFEXXXXXXXXXXXXXGYNPAAVA 617
+GQTREHALLA+ LGVKQL NKMD+T +S+ FE G+NP V
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVP 187
Query: 618 FVAISG 635
FV +SG
Sbjct: 188 FVPVSG 193
Score = 140 bits (338), Expect = 2e-34
Identities = 63/68 (92%), Positives = 66/68 (97%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 92.3 bits (219), Expect = 6e-20
Identities = 43/99 (43%), Positives = 61/99 (61%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
G T+++ FET +++ APGH+ ++ NMI G SQAD VL+++A GEFEAG +
Sbjct: 301 GKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFER 360
Query: 438 NGQTREHALLAFXLGVKQLXRRSNKMDSTEPPYSEPXFE 554
GQTREHA+LA G+ L NKMD +SE ++
Sbjct: 361 GGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYK 399
Score = 79.4 bits (187), Expect = 4e-16
Identities = 34/64 (53%), Positives = 47/64 (73%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 244 AERD 255
ER+
Sbjct: 296 EERE 299
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 91.9 bits (218), Expect = 8e-20
Identities = 53/129 (41%), Positives = 69/129 (53%), Gaps = 1/129 (0%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
G+T+D+A FE+ K I APGHRDFI MI G S AD AVL+V + FE G +
Sbjct: 240 GVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLE 299
Query: 438 NGQTREHALLAFXLGVKQLXRRSNKMDSTEPPYSEPXF-EXXXXXXXXXXXXXGYNPAAV 614
NGQTREHA L LG+ ++ NK+D +SE F E G+ + V
Sbjct: 300 NGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGFKTSNV 357
Query: 615 AFVAISGMA 641
FV IS ++
Sbjct: 358 HFVPISAIS 366
Score = 72.5 bits (170), Expect = 5e-14
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 244 AER 252
ER
Sbjct: 235 EER 237
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 74.9 bits (176), Expect = 9e-15
Identities = 41/95 (43%), Positives = 56/95 (58%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITI A ++ET+ + + PGH D+IKNMITG + D A+++V+A G+
Sbjct: 101 GITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP----- 155
Query: 438 NGQTREHALLAFXLGVKQLXRRSNKMDSTEPPYSE 542
QTREH LLA +GVKQ+ NK+D EP E
Sbjct: 156 --QTREHLLLARQVGVKQIVVYINKVDMVEPDMIE 188
Score = 35.1 bits (77), Expect = 0.009
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 58 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 144
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 30.3 bits (65), Expect = 0.27
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
GITI+ A F +I PGH DF + + D AV I+ G
Sbjct: 78 GITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
Score = 28.7 bits (61), Expect = 0.81
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.9 bits (64), Expect = 0.35
Identities = 20/57 (35%), Positives = 27/57 (47%)
Frame = +3
Query: 288 FETSKYYXTIIXAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 458
FE S Y II PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
Score = 29.1 bits (62), Expect = 0.61
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +3
Query: 315 IIXAPGHRDFIKNMITGTSQADCAVLIV 398
II PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 5.7
Identities = 18/61 (29%), Positives = 25/61 (40%)
Frame = -1
Query: 292 SNFQRAISIVIPSHAQPLVCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWW 113
SN +R ++ V +PK I I++ F P S S C+Y S D W
Sbjct: 13 SNLRRMNRDHSSNNTNRTVLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWR 72
Query: 112 T 110
T
Sbjct: 73 T 73
>SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 96
Score = 25.8 bits (54), Expect = 5.7
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -3
Query: 362 CDHVLDEISVSRSXNDGXIVLASFEL 285
CDH+L IS SR G + FEL
Sbjct: 23 CDHLLQYISNSRDSKAGLKIFLVFEL 48
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 10.0
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 46 PKMGKEKTHINIVVIGHVDSGKST 117
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 10.0
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 193 PGPPSRTSRWYVCQYHHIC 137
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 25.0 bits (52), Expect = 10.0
Identities = 18/65 (27%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = -3
Query: 656 TCXSPCHPRNGHESDSSWVVANLLDV*GXFLLDXLKXGLTVW-WFSGIHFVTPTXELFDT 480
T +PC P + +SSW+ N D+ ++ LTV ++S + V PT D
Sbjct: 367 TLVTPCSPGDPDAFESSWLEVNPEDI--------MEPKLTVRDFYSAVRKVKPTLNAGDI 418
Query: 479 EXESE 465
E ++
Sbjct: 419 EKHTQ 423
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 25.0 bits (52), Expect = 10.0
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +1
Query: 574 PHTSRRLATTQLLSLSWPFLG 636
PH RL ++L LS PFLG
Sbjct: 675 PHDKLRLYALKVLKLSVPFLG 695
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,514,109
Number of Sequences: 5004
Number of extensions: 45413
Number of successful extensions: 155
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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