Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060551.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57970.1 68418.m07253 methyladenine glycosylase family protei... 86 2e-17
At1g15970.1 68414.m01916 methyladenine glycosylase family protei... 84 7e-17
At1g80850.1 68414.m09485 methyladenine glycosylase family protei... 82 3e-16
At5g44680.1 68418.m05474 methyladenine glycosylase family protei... 81 9e-16
At1g75090.1 68414.m08721 methyladenine glycosylase family protei... 79 4e-15
At3g12710.1 68416.m01588 methyladenine glycosylase family protei... 78 6e-15
At1g13635.1 68414.m01602 methyladenine glycosylase family protei... 74 8e-14
>At5g57970.1 68418.m07253 methyladenine glycosylase family protein
similar to SP|P05100 DNA-3-methyladenine glycosylase I
(EC 3.2.2.20) (3-methyladenine-DNA glycosylase I,
constitutive) {Escherichia coli}; contains Pfam profile
PF03352: Methyladenine glycosylase
Length = 347
Score = 86.2 bits (204), Expect = 2e-17
Identities = 38/71 (53%), Positives = 48/71 (67%), Gaps = 2/71 (2%)
Frame = +1
Query: 46 GDNTIKRCEWLS--DDPLYIKYHDEEWGVPQYDSLRLFEMLCLEGQQAGLSWLTVLKKRE 219
G T KRC W++ DP YI +HDEEWGVP +D RLFE+L L G A +W T+L KR+
Sbjct: 149 GSETKKRCTWVTPNSDPCYIVFHDEEWGVPVHDDKRLFELLVLSGALAEHTWPTILSKRQ 208
Query: 220 NYRQVFHGFDP 252
+R+VF FDP
Sbjct: 209 AFREVFADFDP 219
Score = 45.2 bits (102), Expect = 4e-05
Identities = 23/88 (26%), Positives = 46/88 (52%)
Frame = +3
Query: 210 KKRKLQTSFPWFRPVKISNFTKTELLELKKNSSIIRHEGKIQAIFENSLCYLQLVREGTD 389
K++ + F F P I + +++ +S + + K++A+ EN+ L+++ E
Sbjct: 206 KRQAFREVFADFDPNAIVKINEKKIIGPGSPASTLLSDLKLRAVIENARQILKVIEEYGS 265
Query: 390 FSDFIWSFVGKKPIVNYWVQNKDIPKET 473
F +IWSFV K IV+ + + +P +T
Sbjct: 266 FDKYIWSFVKNKAIVSKFRYQRQVPAKT 293
Score = 42.7 bits (96), Expect = 2e-04
Identities = 21/66 (31%), Positives = 33/66 (50%), Gaps = 4/66 (6%)
Frame = +2
Query: 407 EFCWKKTHRKLLGSKQRY----P*RNLNIKGSCKSFEKKGFKFLESKTCYAFMQACGLVN 574
++ W K + SK RY P + + K ++GF+ + Y+FMQA G+ N
Sbjct: 268 KYIWSFVKNKAIVSKFRYQRQVPAKTPKAEVISKDLVRRGFRSVGPTVVYSFMQAAGITN 327
Query: 575 DHIVSC 592
DH+ SC
Sbjct: 328 DHLTSC 333
>At1g15970.1 68414.m01916 methyladenine glycosylase family protein
similar to SP|P05100 DNA-3-methyladenine glycosylase I
(EC 3.2.2.20) (3-methyladenine-DNA glycosylase I,
constitutive) {Escherichia coli}; contains Pfam profile
PF03352: Methyladenine glycosylase
Length = 352
Score = 84.2 bits (199), Expect = 7e-17
Identities = 35/66 (53%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +1
Query: 61 KRCEWLSD--DPLYIKYHDEEWGVPQYDSLRLFEMLCLEGQQAGLSWLTVLKKRENYRQV 234
KRC W++ DP Y+ +HDEEWGVP +D +LFE+LCL G A LSW +L +R R+V
Sbjct: 145 KRCAWITPKADPCYVAFHDEEWGVPVHDDKKLFELLCLSGALAELSWTDILSRRHILREV 204
Query: 235 FHGFDP 252
F FDP
Sbjct: 205 FMDFDP 210
Score = 42.7 bits (96), Expect = 2e-04
Identities = 22/67 (32%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Frame = +2
Query: 407 EFCWKKTHRKLLGSKQRYP*RNLNIKGS-----CKSFEKKGFKFLESKTCYAFMQACGLV 571
++ W + K S+ RY R + +K S K ++GF+ + Y+FMQA GL
Sbjct: 259 KYMWNFVNNKPTQSQFRYQ-RQVPVKTSKAEFISKDLVRRGFRSVSPTVIYSFMQAAGLT 317
Query: 572 NDHIVSC 592
NDH++ C
Sbjct: 318 NDHLIGC 324
Score = 39.1 bits (87), Expect = 0.003
Identities = 21/89 (23%), Positives = 43/89 (48%)
Frame = +3
Query: 210 KKRKLQTSFPWFRPVKISNFTKTELLELKKNSSIIRHEGKIQAIFENSLCYLQLVREGTD 389
++ L+ F F PV ++ +L + + E KI++I +NS +++ E
Sbjct: 197 RRHILREVFMDFDPVAVAELNDKKLTAPGTAAISLLSEVKIRSILDNSRHVRKIIAECGS 256
Query: 390 FSDFIWSFVGKKPIVNYWVQNKDIPKETS 476
++W+FV KP + + + +P +TS
Sbjct: 257 LKKYMWNFVNNKPTQSQFRYQRQVPVKTS 285
>At1g80850.1 68414.m09485 methyladenine glycosylase family protein
similar to SP|P05100 DNA-3-methyladenine glycosylase I
(EC 3.2.2.20) (3-methyladenine-DNA glycosylase I,
constitutive) {Escherichia coli}; contains Pfam profile
PF03352: Methyladenine glycosylase
Length = 327
Score = 82.2 bits (194), Expect = 3e-16
Identities = 36/66 (54%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +1
Query: 61 KRCEWLS--DDPLYIKYHDEEWGVPQYDSLRLFEMLCLEGQQAGLSWLTVLKKRENYRQV 234
KRC W++ D YI +HDEEWGVP +D RLFE+L L G A LSW +L KR+ +R+V
Sbjct: 134 KRCAWITPKSDQCYIAFHDEEWGVPVHDDKRLFELLSLSGALAELSWKDILSKRQLFREV 193
Query: 235 FHGFDP 252
F FDP
Sbjct: 194 FMDFDP 199
Score = 44.8 bits (101), Expect = 5e-05
Identities = 22/89 (24%), Positives = 47/89 (52%)
Frame = +3
Query: 210 KKRKLQTSFPWFRPVKISNFTKTELLELKKNSSIIRHEGKIQAIFENSLCYLQLVREGTD 389
K++ + F F P+ IS T ++ + ++ + E K+++I EN+ +++
Sbjct: 186 KRQLFREVFMDFDPIAISELTNKKITSPEIAATTLLSEQKLRSILENANQVCKIIGAFGS 245
Query: 390 FSDFIWSFVGKKPIVNYWVQNKDIPKETS 476
F +IW+FV +KP + + + +P +TS
Sbjct: 246 FDKYIWNFVNQKPTQSQFRYPRQVPVKTS 274
Score = 43.2 bits (97), Expect = 2e-04
Identities = 22/67 (32%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Frame = +2
Query: 407 EFCWKKTHRKLLGSKQRYP*RNLNIKGS-----CKSFEKKGFKFLESKTCYAFMQACGLV 571
++ W ++K S+ RYP R + +K S K ++GF+ + Y+FMQ GL
Sbjct: 248 KYIWNFVNQKPTQSQFRYP-RQVPVKTSKAELISKDLVRRGFRSVSPTVIYSFMQTAGLT 306
Query: 572 NDHIVSC 592
NDH+ C
Sbjct: 307 NDHLTCC 313
>At5g44680.1 68418.m05474 methyladenine glycosylase family protein
similar to SP|P05100 DNA-3-methyladenine glycosylase I
(EC 3.2.2.20) (3-methyladenine-DNA glycosylase I,
constitutive) {Escherichia coli}; contains Pfam profile
PF03352: Methyladenine glycosylase
Length = 353
Score = 80.6 bits (190), Expect = 9e-16
Identities = 33/65 (50%), Positives = 45/65 (69%), Gaps = 2/65 (3%)
Frame = +1
Query: 61 KRCEWL--SDDPLYIKYHDEEWGVPQYDSLRLFEMLCLEGQQAGLSWLTVLKKRENYRQV 234
KRC ++ S DP+Y+ YHD+EWGVP +D LFE+L L G Q G W +VLK+R +R+
Sbjct: 164 KRCSFITTSSDPIYVAYHDKEWGVPVHDDNLLFELLVLTGAQVGSDWTSVLKRRNTFREA 223
Query: 235 FHGFD 249
F GF+
Sbjct: 224 FSGFE 228
Score = 39.9 bits (89), Expect = 0.002
Identities = 16/49 (32%), Positives = 28/49 (57%)
Frame = +2
Query: 446 SKQRYP*RNLNIKGSCKSFEKKGFKFLESKTCYAFMQACGLVNDHIVSC 592
S Q+ P + + K ++GF+F+ ++ MQA GL NDH+++C
Sbjct: 293 SCQKIPVKTSKSETISKDMVRRGFRFVGPTVIHSLMQAAGLTNDHLITC 341
Score = 37.5 bits (83), Expect = 0.008
Identities = 18/89 (20%), Positives = 46/89 (51%)
Frame = +3
Query: 210 KKRKLQTSFPWFRPVKISNFTKTELLELKKNSSIIRHEGKIQAIFENSLCYLQLVREGTD 389
++ + +F F +++F + ++ + + I + ++ A+ +N+ L++ R+
Sbjct: 216 RRNTFREAFSGFEAELVADFNEKKIQSIVNDYGI--NLSQVLAVVDNAKQILKVKRDLGS 273
Query: 390 FSDFIWSFVGKKPIVNYWVQNKDIPKETS 476
F+ +IW F+ KP+ + + IP +TS
Sbjct: 274 FNKYIWGFMKHKPVTTKYTSCQKIPVKTS 302
>At1g75090.1 68414.m08721 methyladenine glycosylase family protein
similar to SP|P05100 DNA-3-methyladenine glycosylase I
(EC 3.2.2.20) (3-methyladenine-DNA glycosylase I,
constitutive) {Escherichia coli}; contains Pfam profile
PF03352: Methyladenine glycosylase
Length = 329
Score = 78.6 bits (185), Expect = 4e-15
Identities = 30/67 (44%), Positives = 47/67 (70%), Gaps = 2/67 (2%)
Frame = +1
Query: 58 IKRCEWLS--DDPLYIKYHDEEWGVPQYDSLRLFEMLCLEGQQAGLSWLTVLKKRENYRQ 231
+KRC W++ DP+Y+ +HDEEWGVP D +LFE+L A SW ++L++R+++R+
Sbjct: 118 VKRCHWITPNSDPIYVLFHDEEWGVPVRDDKKLFELLVFSQALAEFSWPSILRRRDDFRK 177
Query: 232 VFHGFDP 252
+F FDP
Sbjct: 178 LFEEFDP 184
Score = 56.8 bits (131), Expect = 1e-08
Identities = 27/77 (35%), Positives = 44/77 (57%)
Frame = +3
Query: 234 FPWFRPVKISNFTKTELLELKKNSSIIRHEGKIQAIFENSLCYLQLVREGTDFSDFIWSF 413
F F P I+ FT+ L+ L+ N +I E K++AI EN+ L++ +E FS++ W F
Sbjct: 179 FEEFDPSAIAQFTEKRLMSLRVNGCLILSEQKLRAIVENAKSVLKVKQEFGSFSNYCWRF 238
Query: 414 VGKKPIVNYWVQNKDIP 464
V KP+ N + + +P
Sbjct: 239 VNHKPLRNGYRYGRQVP 255
Score = 47.6 bits (108), Expect = 8e-06
Identities = 26/92 (28%), Positives = 45/92 (48%), Gaps = 4/92 (4%)
Frame = +2
Query: 401 YLEFCWKKTHRKLLGSKQRY----P*RNLNIKGSCKSFEKKGFKFLESKTCYAFMQACGL 568
+ +CW+ + K L + RY P ++ + K ++GF+ + Y+F+QA G+
Sbjct: 231 FSNYCWRFVNHKPLRNGYRYGRQVPVKSPKAEYISKDMMQRGFRCVGPTVMYSFLQASGI 290
Query: 569 VNDHIVSCICRQTLQXSTID*SQAQSHSLPTK 664
VNDH+ +C Q T + +SH TK
Sbjct: 291 VNDHLTACFRYQECNVETE--RETKSHETETK 320
>At3g12710.1 68416.m01588 methyladenine glycosylase family protein
similar to SP|P05100 DNA-3-methyladenine glycosylase I
(EC 3.2.2.20) (3-methyladenine-DNA glycosylase I,
constitutive) {Escherichia coli}; contains Pfam profile
PF03352: Methyladenine glycosylase
Length = 312
Score = 77.8 bits (183), Expect = 6e-15
Identities = 32/65 (49%), Positives = 44/65 (67%), Gaps = 2/65 (3%)
Frame = +1
Query: 61 KRCEWLS--DDPLYIKYHDEEWGVPQYDSLRLFEMLCLEGQQAGLSWLTVLKKRENYRQV 234
+RC +L+ DP+Y+ YHDEEWGVP +D LFE+L L G Q G W + L+KR +YR+
Sbjct: 122 QRCSFLTPTSDPIYVAYHDEEWGVPVHDDKTLFELLTLSGAQVGSDWTSTLRKRHDYRKA 181
Query: 235 FHGFD 249
F F+
Sbjct: 182 FMEFE 186
Score = 41.9 bits (94), Expect = 4e-04
Identities = 16/36 (44%), Positives = 25/36 (69%)
Frame = +2
Query: 494 KSFEKKGFKFLESKTCYAFMQACGLVNDHIVSCICR 601
K ++GF+F+ ++FMQA GL NDH+++C CR
Sbjct: 267 KDMVRRGFRFVGPTVVHSFMQAAGLTNDHLITC-CR 301
Score = 34.7 bits (76), Expect = 0.058
Identities = 22/90 (24%), Positives = 43/90 (47%), Gaps = 1/90 (1%)
Frame = +3
Query: 210 KKRKLQTSFPWFRPVKISNFTKTELLELKKNSSIIRHEGKIQAIFENSLCYLQLVREGTD 389
K+ + +F F ++ T+ E+ + I K++ + EN+ +++ +
Sbjct: 174 KRHDYRKAFMEFEAEVVAKLTEKEMNAISIEYKI--EMSKVRGVVENAKKIVEIKKAFVS 231
Query: 390 FSDFIWSFVGKKPI-VNYWVQNKDIPKETS 476
++W FV KPI NY + +K IP +TS
Sbjct: 232 LEKYLWGFVNHKPISTNYKLGHK-IPVKTS 260
>At1g13635.1 68414.m01602 methyladenine glycosylase family protein
Contains Pfam profile PF03352: Methyladenine
glycosylase
Length = 311
Score = 74.1 bits (174), Expect = 8e-14
Identities = 28/66 (42%), Positives = 43/66 (65%), Gaps = 2/66 (3%)
Frame = +1
Query: 61 KRCEWLS--DDPLYIKYHDEEWGVPQYDSLRLFEMLCLEGQQAGLSWLTVLKKRENYRQV 234
KRC W++ D +Y+ +HD++WGVP YD LFE L + G +W +LK++E++R+
Sbjct: 115 KRCNWITKKSDEVYVMFHDQQWGVPVYDDNLLFEFLAMSGMLMDYNWTEILKRKEHFREA 174
Query: 235 FHGFDP 252
F FDP
Sbjct: 175 FCEFDP 180
Score = 62.1 bits (144), Expect = 3e-10
Identities = 24/85 (28%), Positives = 50/85 (58%)
Frame = +3
Query: 210 KKRKLQTSFPWFRPVKISNFTKTELLELKKNSSIIRHEGKIQAIFENSLCYLQLVREGTD 389
+K + +F F P +++ + E+ E+ N +I+ E +++ I +N+ C ++V E
Sbjct: 167 RKEHFREAFCEFDPNRVAKMGEKEIAEIASNKAIMLQESRVRCIVDNAKCITKVVNEFGS 226
Query: 390 FSDFIWSFVGKKPIVNYWVQNKDIP 464
FS F+W F+ KPI+N + ++++P
Sbjct: 227 FSSFVWGFMDYKPIINKFKYSRNVP 251
Score = 37.9 bits (84), Expect = 0.006
Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 4/68 (5%)
Frame = +2
Query: 401 YLEFCWKKTHRKLLGSKQRY----P*RNLNIKGSCKSFEKKGFKFLESKTCYAFMQACGL 568
+ F W K + +K +Y P R+ + K K+GF+F+ ++FMQA GL
Sbjct: 227 FSSFVWGFMDYKPIINKFKYSRNVPLRSPKAEIISKDMIKRGFRFVGPVIVHSFMQAAGL 286
Query: 569 VNDHIVSC 592
DH+V C
Sbjct: 287 TIDHLVDC 294
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,794,060
Number of Sequences: 28952
Number of extensions: 273730
Number of successful extensions: 624
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 598
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 624
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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