BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060549.seq (687 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 227 6e-62 AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 227 6e-62 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 220 9e-60 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 220 1e-59 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2 AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase... 24 1.6 DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.7 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6 DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450 monoo... 23 3.6 DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. 22 4.8 DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. 21 8.3 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 8.3 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 8.3 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 227 bits (556), Expect = 6e-62 Identities = 110/139 (79%), Positives = 118/139 (84%) Frame = +2 Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72 Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVA 616 NGQTREHALLAFTLGVKQLIVGVNK + +++ +++GYNPAAVA Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 132 Query: 617 FVPISGWHGDNMLEPSTKM 673 FVPISGWHGDNMLE S+KM Sbjct: 133 FVPISGWHGDNMLEVSSKM 151 Score = 28.3 bits (60), Expect = 0.072 Identities = 11/12 (91%), Positives = 12/12 (100%) Frame = +1 Query: 223 WVLDKLKAERDR 258 WVLDKLKAER+R Sbjct: 1 WVLDKLKAERER 12 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 227 bits (556), Expect = 6e-62 Identities = 110/139 (79%), Positives = 118/139 (84%) Frame = +2 Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129 Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVA 616 NGQTREHALLAFTLGVKQLIVGVNK + +++ +++GYNPAAVA Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 189 Query: 617 FVPISGWHGDNMLEPSTKM 673 FVPISGWHGDNMLE S+KM Sbjct: 190 FVPISGWHGDNMLEVSSKM 208 Score = 146 bits (354), Expect = 2e-37 Identities = 67/69 (97%), Positives = 68/69 (98%) Frame = +1 Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 232 DKLKAERDR 258 DKLKAER+R Sbjct: 61 DKLKAERER 69 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 220 bits (538), Expect = 9e-60 Identities = 106/138 (76%), Positives = 116/138 (84%) Frame = +2 Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436 GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISK Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129 Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVA 616 NGQTREHALLAFTLGVKQLIVGVNK + + +++ +++GYN A+VA Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVA 189 Query: 617 FVPISGWHGDNMLEPSTK 670 FVPISGWHGDNMLEPS K Sbjct: 190 FVPISGWHGDNMLEPSPK 207 Score = 146 bits (354), Expect = 2e-37 Identities = 67/69 (97%), Positives = 68/69 (98%) Frame = +1 Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 232 DKLKAERDR 258 DKLKAER+R Sbjct: 61 DKLKAERER 69 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 220 bits (537), Expect = 1e-59 Identities = 106/135 (78%), Positives = 114/135 (84%) Frame = +2 Query: 269 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 448 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 449 REHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVAFVPI 628 REHALLAFTLGVKQLIVGVNK + +++ +++GYNPAAVAFVPI Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120 Query: 629 SGWHGDNMLEPSTKM 673 SGWHGDNMLE S+KM Sbjct: 121 SGWHGDNMLEVSSKM 135 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 33.1 bits (72), Expect = 0.003 Identities = 16/35 (45%), Positives = 18/35 (51%) Frame = +2 Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412 VT +D PGH FI G D VL+VAA G Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229 Score = 25.4 bits (53), Expect = 0.51 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +1 Query: 64 KTHINIVVIGHVDSGKST 117 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.2 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +1 Query: 73 INIVVIGHVDSGKST 117 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase protein. Length = 1143 Score = 23.8 bits (49), Expect = 1.6 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = -2 Query: 641 RAIQKWARKRQQLGCSPIFLMYEDTSFLISSNLGS 537 +A +KWA + C L +DT ++ +LGS Sbjct: 652 QAFRKWAADTFAVACETFCLDDDDTLLEVALSLGS 686 >DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monooxygenase protein. Length = 517 Score = 23.0 bits (47), Expect = 2.7 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = -2 Query: 65 FSLPIFG*SRITNCV*Y 15 FSLPIFG I +C+ Y Sbjct: 57 FSLPIFGTRWIFSCIGY 73 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 2.7 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 3.6 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450 monooxygenase protein. Length = 499 Score = 22.6 bits (46), Expect = 3.6 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = -2 Query: 260 YRSRSAFSLSNTQAYLKDPLPISWASFSN 174 Y +RS F L N +KD L ++ F+N Sbjct: 76 YATRSPFLLLNDPELIKDILIRDFSKFAN 104 >DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. Length = 495 Score = 22.2 bits (45), Expect = 4.8 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = -3 Query: 307 IVLASFELPESNIDCDTDHAQPLVCPIPKHI*RILY 200 IV+ FE+ +S D + A IP ++ R+L+ Sbjct: 54 IVIGGFEIEKSEDDSFNNQADKSEKRIPLYVCRVLH 89 Score = 21.4 bits (43), Expect = 8.3 Identities = 24/107 (22%), Positives = 46/107 (42%), Gaps = 3/107 (2%) Frame = -3 Query: 532 MVVQWNPFVYSYDELFDTEGESEQGMLTGLTVLRDTSFEFTGTGSYDEHSAISLRGSCDH 353 M V ++ + S+ E +T+ G+ + T+ D EF +S + + Sbjct: 329 MEVPYSGTLISHYEDGETKSRFISGIRSEQTIF-DVKPEFGSIYFLGNYSLVPTTTASPT 387 Query: 352 VLDEISVSRSINDGNI---VLASFELPESNIDCDTDHAQPLVCPIPK 221 + S +I+ +I V+ L E N+D + DH + ++ P PK Sbjct: 388 TEPSTTTSTTISQKHIKVFVVNKDILHEHNVDDNEDHDENMIIP-PK 433 >DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. Length = 828 Score = 21.4 bits (43), Expect = 8.3 Identities = 9/28 (32%), Positives = 16/28 (57%) Frame = +1 Query: 487 TAHRRSKQMDSTEPPYSEPRFEEIKKEV 570 T+ R++ S PP ++PR E + + V Sbjct: 725 TSLERTQPTMSQMPPTAQPRMERLAEAV 752 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 8.3 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -3 Query: 670 FG*RLQHVVSVPSRNGHESDSSWV 599 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 8.3 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +2 Query: 512 WIPLNHHTVSPDLRKSRRKY 571 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 200,315 Number of Sequences: 438 Number of extensions: 4302 Number of successful extensions: 35 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 22 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 27 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 20952180 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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