Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060541.seq
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 124 7e-29
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 124 7e-29
At1g59850.1 68414.m06741 expressed protein 31 0.71
At5g05790.1 68418.m00637 myb family transcription factor contain... 29 3.8
At3g11960.2 68416.m01476 cleavage and polyadenylation specificit... 29 3.8
At3g11960.1 68416.m01475 cleavage and polyadenylation specificit... 29 3.8
At4g05420.1 68417.m00824 UV-damaged DNA-binding protein, putativ... 28 5.0
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B subunit
3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 124 bits (298), Expect = 7e-29
Identities = 53/84 (63%), Positives = 67/84 (79%)
Frame = +3
Query: 255 LNGASQKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFF 434
LNGA K D +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L FTSR+D DFF
Sbjct: 1080 LNGAPNKVDEIVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFF 1139
Query: 435 QHLEMHMRSENSPLCGRDHLSFRS 506
HLEMHMR E PLCGRDH+++RS
Sbjct: 1140 SHLEMHMRQEYPPLCGRDHMAYRS 1163
Score = 105 bits (252), Expect = 3e-23
Identities = 46/86 (53%), Positives = 64/86 (74%)
Frame = +1
Query: 1 RHEIFVSDVQESVFCVKHKKRENQLIIFADDTNPRWITNSCILDYDTIAVSDKFGNVAIM 180
R I+V D+QES K+++ ENQL IFADD PRW+T S +D+DT+A +DKFGNV +
Sbjct: 995 RDRIYVGDIQESFHYCKYRRDENQLYIFADDCVPRWLTASHHVDFDTMAGADKFGNVYFV 1054
Query: 181 RLPQSVSDDVDEDPTGNKALWDRGFL 258
RLPQ +S++++EDPTG K W++G L
Sbjct: 1055 RLPQDLSEEIEEDPTGGKIKWEQGKL 1080
Score = 59.3 bits (137), Expect = 2e-09
Identities = 26/41 (63%), Positives = 33/41 (80%)
Frame = +2
Query: 509 YYPVKNVIDGDLCEQFNSLDPGKQKAIAXDLERTPAEVSKK 631
Y+PVK+VIDGDLCEQF +L Q+ IA +L+RTPAE+ KK
Sbjct: 1165 YFPVKDVIDGDLCEQFPTLPMDLQRKIADELDRTPAEILKK 1205
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B subunit
3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 124 bits (298), Expect = 7e-29
Identities = 53/84 (63%), Positives = 67/84 (79%)
Frame = +3
Query: 255 LNGASQKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFF 434
LNGA K D +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L FTSR+D DFF
Sbjct: 1080 LNGAPNKVDEIVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFF 1139
Query: 435 QHLEMHMRSENSPLCGRDHLSFRS 506
HLEMHMR E PLCGRDH+++RS
Sbjct: 1140 SHLEMHMRQEYPPLCGRDHMAYRS 1163
Score = 105 bits (252), Expect = 3e-23
Identities = 46/86 (53%), Positives = 64/86 (74%)
Frame = +1
Query: 1 RHEIFVSDVQESVFCVKHKKRENQLIIFADDTNPRWITNSCILDYDTIAVSDKFGNVAIM 180
R I+V D+QES K+++ ENQL IFADD PRW+T S +D+DT+A +DKFGNV +
Sbjct: 995 RDRIYVGDIQESFHYCKYRRDENQLYIFADDCVPRWLTASHHVDFDTMAGADKFGNVYFV 1054
Query: 181 RLPQSVSDDVDEDPTGNKALWDRGFL 258
RLPQ +S++++EDPTG K W++G L
Sbjct: 1055 RLPQDLSEEIEEDPTGGKIKWEQGKL 1080
Score = 59.3 bits (137), Expect = 2e-09
Identities = 26/41 (63%), Positives = 33/41 (80%)
Frame = +2
Query: 509 YYPVKNVIDGDLCEQFNSLDPGKQKAIAXDLERTPAEVSKK 631
Y+PVK+VIDGDLCEQF +L Q+ IA +L+RTPAE+ KK
Sbjct: 1165 YFPVKDVIDGDLCEQFPTLPMDLQRKIADELDRTPAEILKK 1205
>At1g59850.1 68414.m06741 expressed protein
Length = 498
Score = 31.1 bits (67), Expect = 0.71
Identities = 25/74 (33%), Positives = 34/74 (45%)
Frame = -1
Query: 251 PLSQSALFPVGSSSTSSLTDCGNRIIATLPNLSDTAIVS*SRIQLFVIHLGLVSSAKIIN 72
P Q P S ++TD R+IA L LSD ++ + +L I L L
Sbjct: 2 PSVQIRSSPSHSQPAMTVTDLKQRVIACLNRLSDRDTLALAAAELDSIALNLSPET---- 57
Query: 71 *FSLFLCLTQNTDS 30
FSLF+ Q+TDS
Sbjct: 58 -FSLFINCLQSTDS 70
>At5g05790.1 68418.m00637 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 277
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 49 KHKKRENQLIIFADDTNPRWITNSCILDYDTIA 147
++KK E L ++ADDT RW + ++ TI+
Sbjct: 35 ENKKFERALAVYADDTPDRWFKVAAMIPGKTIS 67
>At3g11960.2 68416.m01476 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1329
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/80 (21%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +3
Query: 270 QKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLE- 446
+KG + + + S + I + ++ T+ GS+ V P +S E+++ + ++
Sbjct: 1180 KKGCNIYKLPADDVLRSYGLSKSIDTADDTIIAGTLLGSIFVFAPISS-EEYELLEGVQA 1238
Query: 447 -MHMRSENSPLCGRDHLSFR 503
+ + +P+ G DH FR
Sbjct: 1239 KLGIHPLTAPVLGNDHNEFR 1258
>At3g11960.1 68416.m01475 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1379
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/80 (21%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +3
Query: 270 QKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLE- 446
+KG + + + S + I + ++ T+ GS+ V P +S E+++ + ++
Sbjct: 1230 KKGCNIYKLPADDVLRSYGLSKSIDTADDTIIAGTLLGSIFVFAPISS-EEYELLEGVQA 1288
Query: 447 -MHMRSENSPLCGRDHLSFR 503
+ + +P+ G DH FR
Sbjct: 1289 KLGIHPLTAPVLGNDHNEFR 1308
>At4g05420.1 68417.m00824 UV-damaged DNA-binding protein, putative
similar to UV-damaged DNA binding protein (GI:12082087)
[Oryza sativa]; contains Pfam PF03178 : CPSF A subunit
region
Length = 1088
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/67 (23%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 10 IFVSDVQESVFCVKHKKRENQLIIFADDTNPRWITNSCILDYDT-IAVSDKFGNVAIMRL 186
I V D+ +S+ + +K E + A D N W++ ILD D + + F + + +
Sbjct: 880 IVVGDLMKSISLLLYKHEEGAIEERARDYNANWMSAVEILDDDIYLGAENNFNLLTVKKN 939
Query: 187 PQSVSDD 207
+ +D+
Sbjct: 940 SEGATDE 946
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,317,486
Number of Sequences: 28952
Number of extensions: 294524
Number of successful extensions: 706
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 691
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 706
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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