Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060539.seq
         (686 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      51   2e-07
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    44   2e-05
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    43   4e-05
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar...    35   0.013
SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr 3||...    27   3.4  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    27   3.4  
SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosacch...    25   7.8  

>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 24/89 (26%), Positives = 41/89 (46%), Gaps = 1/89 (1%)
 Frame = +3

Query: 213 FCL-CPVLSHVS*WCFYEFEGRKYCEQDFQVLFAPCCAKCGEFVIGRVIKAMNSNWHPAC 389
           FC  C  + +V+  C Y  +   +C+  +   +A  C KC + ++G  +K  +  +H  C
Sbjct: 343 FCAGCSEVFNVNIPCIYR-DDLYWCQTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQC 401

Query: 390 FRCEECNVELADAGFIKHAGRALCHVCNA 476
           + C  CN  L D G+       +C  C A
Sbjct: 402 WTCGACNALLGDEGYFMIENTPICRPCKA 430



 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
 Frame = +3

Query: 318 CAKCGEFV-IGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALCHV 467
           C  CG  +  GR+I A     HP CF+C+ C+  L   GF    G+  CH+
Sbjct: 258 CHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308



 Score = 34.3 bits (75), Expect = 0.017
 Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 4/62 (6%)
 Frame = +3

Query: 231 LSHVS*WCFYEFEGRKYCEQDFQVLFAPCCAKCGEFVIGRVIKAMNSNW----HPACFRC 398
           L HV    FY  EG+ YC  D+   F+P C  C   +  + +  +N++W    H  C  C
Sbjct: 292 LEHVG---FYYREGKFYCHLDYHEQFSPRCKHCKTPIEDQAVH-INNDWFHENHHFCAGC 347

Query: 399 EE 404
            E
Sbjct: 348 SE 349



 Score = 25.8 bits (54), Expect = 5.9
 Identities = 13/47 (27%), Positives = 21/47 (44%)
 Frame = +1

Query: 136 CTRCGDGFEPNEKIVNSNGELWHTNCFVCAQCFRMFPDGVSMNLRDE 276
           C  C    E  ++ V+ N + +H N   CA C  +F   +    RD+
Sbjct: 318 CKHCKTPIE--DQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDD 362


>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 15/39 (38%), Positives = 25/39 (64%)
 Frame = +3

Query: 318 CAKCGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGF 434
           CA CG+ + G+ ++A+ + +H  CFRC +CN  +A   F
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFF 154



 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +3

Query: 255 FYEFEGRKYCEQDFQVLFAPCCAKCGEFVIGRVIK----AMNSNWHPAC 389
           +YE+EG+ YC   +  LFA  C  C   ++ + ++     ++ NWH  C
Sbjct: 219 YYEYEGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 19/62 (30%), Positives = 28/62 (45%), Gaps = 1/62 (1%)
 Frame = +3

Query: 282 CEQDFQVLFAPCCAKCGEFVIGRVIKAMNSNWHPACFRCEEC-NVELADAGFIKHAGRAL 458
           CE D+       CA CG  + G  I A+N  +H   F C  C  V   +  + ++ G+  
Sbjct: 168 CETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVY 227

Query: 459 CH 464
           CH
Sbjct: 228 CH 229



 Score = 25.4 bits (53), Expect = 7.8
 Identities = 10/32 (31%), Positives = 15/32 (46%)
 Frame = +1

Query: 136 CTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 231
           C  CG     + + V + G ++H  CF C  C
Sbjct: 116 CASCGQVI--SGQYVRALGNIYHLECFRCHDC 145


>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 15/45 (33%), Positives = 24/45 (53%)
 Frame = +1

Query: 97  SLFLATMSLDNMYCTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 231
           S+  +  S  +  C RCG  F+  E  ++  G +WH +CF C +C
Sbjct: 6   SISKSPSSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKC 50



 Score = 34.3 bits (75), Expect = 0.017
 Identities = 13/54 (24%), Positives = 24/54 (44%)
 Frame = +3

Query: 309 APCCAKCGEFVIGRVIKAMNSNWHPACFRCEECNVELADAGFIKHAGRALCHVC 470
           A  C  C   +    + +   ++H  CFRC +C  ++ D+ F +      C+ C
Sbjct: 75  AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128



 Score = 29.1 bits (62), Expect = 0.63
 Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 5/56 (8%)
 Frame = +3

Query: 318 CAKCGEFVIGRV--IKAMNSNWHPACFRCEECN--VELADAGFIKHA-GRALCHVC 470
           C +CG+    R   I      WH  CF C +C+  +E +D   ++ + GR +C  C
Sbjct: 19  CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC 74


>SPBC28E12.03 |rga4||GTPase activating protein
           Rga4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 933

 Score = 34.7 bits (76), Expect = 0.013
 Identities = 15/44 (34%), Positives = 22/44 (50%)
 Frame = +1

Query: 100 LFLATMSLDNMYCTRCGDGFEPNEKIVNSNGELWHTNCFVCAQC 231
           L L T S    +C +C +   P+   V   G+ WH++CF C  C
Sbjct: 12  LSLETPSERTCFCIKCWESV-PSTSQVWFGGKCWHSDCFKCVNC 54



 Score = 28.3 bits (60), Expect = 1.1
 Identities = 13/35 (37%), Positives = 15/35 (42%), Gaps = 1/35 (2%)
 Frame = +3

Query: 318 CAKCGEFVIGRVIKAMNSN-WHPACFRCEECNVEL 419
           C KC E V            WH  CF+C  CN +L
Sbjct: 24  CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKL 58


>SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 465

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = +2

Query: 533 DGEPLRYRGEVYHGLSLYLWLPV 601
           + E L +R + YH L ++L+LPV
Sbjct: 261 EDEVLNFRRKTYHALVVFLFLPV 283


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 18/74 (24%), Positives = 34/74 (45%), Gaps = 1/74 (1%)
 Frame = -2

Query: 427 ASASSTLHSSHRKQAGCQLEFIAFITRPITNSPHFAQHGANKTWKSCSQYFRPSNS*KHH 248
           +S SST+ SS    +       +  +RP ++S H +   ++K+  S      P +S  +H
Sbjct: 366 SSFSSTVSSSSSTSSSTLTSSSSSSSRPASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYH 425

Query: 247 QET-CESTGHKQNN 209
             T   S+ H  ++
Sbjct: 426 NSTSSRSSSHSSSH 439


>SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 213

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 12/34 (35%), Positives = 20/34 (58%), Gaps = 2/34 (5%)
 Frame = +1

Query: 109 ATMSLDNMYCTRCGDG--FEPNEKIVNSNGELWH 204
           AT S+ N +CT+C D   F   ++I+ ++ E  H
Sbjct: 58  ATNSIINKFCTQCSDNDEFPTPKQIMETDVETLH 91


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,110,943
Number of Sequences: 5004
Number of extensions: 69684
Number of successful extensions: 203
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)