BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060529.seq (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 31 0.20 SPAC19B12.10 |sst2||human AMSH protein homolog|Schizosaccharomyc... 29 0.80 SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 28 1.4 SPCC1393.10 |ctr4||copper transporter complex subunit Ctr4 |Schi... 28 1.4 SPAC13G6.08 |||Cdc20/Fizzy family WD repeat protein|Schizosaccha... 27 2.4 SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 26 4.3 SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharo... 25 7.5 SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.9 SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 9.9 SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 25 9.9 >SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1402 Score = 30.7 bits (66), Expect = 0.20 Identities = 17/55 (30%), Positives = 29/55 (52%), Gaps = 5/55 (9%) Frame = -3 Query: 197 RFILVLFLFHFEHDCFRIS-VVRHLYIR----IFKILLYALYNDYLINYLFTYVF 48 RF+ L L H D R++ +V + + + F + Y +YN++ NYLF Y + Sbjct: 1049 RFLSKLVLVHGRWDYNRVAEMVNNFFYKSVVWTFTLFWYQIYNNFDANYLFDYTY 1103 >SPAC19B12.10 |sst2||human AMSH protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 28.7 bits (61), Expect = 0.80 Identities = 28/95 (29%), Positives = 37/95 (38%) Frame = +2 Query: 245 LIKSSNSDSEQWVEKESSIVQKETREDWMAMTGLLKTYSKQDIKPPRDVKKKNGIDSYNP 424 L K S DS+ +EK S V + E W + S D+ P D +K + S P Sbjct: 122 LKKLSMKDSQPSLEKPVSYVDEPILEQWALSDLQILPPSSTDLLSP-DSQKLSKSSSDLP 180 Query: 425 STSSRELNPYWKDGGSGLPQTPESFRKADLSSNHL 529 LN S LP + F K LS + L Sbjct: 181 QFDYPSLNSS-PTFNSNLPISSSRFEKTSLSDSKL 214 >SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein Mug36|Schizosaccharomyces pombe|chr 2|||Manual Length = 1646 Score = 27.9 bits (59), Expect = 1.4 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = +2 Query: 248 IKSSNSDSEQWVEKESSIVQKETREDWM 331 I SSN + E + S+ QK+TR W+ Sbjct: 285 IISSNREEESFYSVHDSVTQKKTRTSWL 312 >SPCC1393.10 |ctr4||copper transporter complex subunit Ctr4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 289 Score = 27.9 bits (59), Expect = 1.4 Identities = 17/50 (34%), Positives = 28/50 (56%) Frame = -3 Query: 188 LVLFLFHFEHDCFRISVVRHLYIRIFKILLYALYNDYLINYLFTYVFYDF 39 L +FL HF CF + V+++ + +LL YN Y+I +LF F+ + Sbjct: 200 LRIFL-HFLRSCFYL--VQYI-VAYIAMLLAMYYNGYVILFLFCGTFFGY 245 >SPAC13G6.08 |||Cdc20/Fizzy family WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 535 Score = 27.1 bits (57), Expect = 2.4 Identities = 12/45 (26%), Positives = 23/45 (51%) Frame = +2 Query: 308 KETREDWMAMTGLLKTYSKQDIKPPRDVKKKNGIDSYNPSTSSRE 442 KET L ++Y K + + + +NG+D + P TS+++ Sbjct: 52 KETANVCPISKNLFQSYPKGSYRNSQRLTSRNGLDRFIPMTSNKD 96 >SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 26.2 bits (55), Expect = 4.3 Identities = 16/52 (30%), Positives = 27/52 (51%) Frame = +2 Query: 272 EQWVEKESSIVQKETREDWMAMTGLLKTYSKQDIKPPRDVKKKNGIDSYNPS 427 EQW++ +S ++ + + GLL SK+ K R+ +K + DS N S Sbjct: 470 EQWLKDKSKKARRRAEREKLRSEGLLGKKSKK--KLLRESQKPSSSDSDNAS 519 >SPAC7D4.06c |alg3||dolichol-P-Man dependent alpha|Schizosaccharomyces pombe|chr 1|||Manual Length = 406 Score = 25.4 bits (53), Expect = 7.5 Identities = 18/52 (34%), Positives = 26/52 (50%) Frame = -3 Query: 188 LVLFLFHFEHDCFRISVVRHLYIRIFKILLYALYNDYLINYLFTYVFYDFSC 33 +V +LF F I V+ L R+ I + L+ND N LF+ +F SC Sbjct: 119 IVGYLFKIVRAPFYIYVLLILSKRLHSIFILRLFNDG-FNSLFSSLFILSSC 169 >SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 977 Score = 25.0 bits (52), Expect = 9.9 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = +2 Query: 416 YNPSTSSRELNPYWKDGGSGLPQTPESFRKADLSSN 523 +NP +P ++G S + P S ++ DLSSN Sbjct: 92 FNPFEFLEATSPLQQNGKSRDTEKPPSMKEKDLSSN 127 >SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 188 Score = 25.0 bits (52), Expect = 9.9 Identities = 13/36 (36%), Positives = 17/36 (47%) Frame = -3 Query: 233 CFFFTLKLFRLMRFILVLFLFHFEHDCFRISVVRHL 126 CFFF LF F+ F+F FR+ +HL Sbjct: 109 CFFFYFSLFSFFSFLFTSLHFNF---FFRLCRHKHL 141 >SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 710 Score = 25.0 bits (52), Expect = 9.9 Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Frame = +2 Query: 431 SSRELNPY-WKDGGSGLPQTPESFRKADLSSNHLKKMTTIHYLLEV 565 S E++P +K GG+ TPE+ + + N K ++T + +L++ Sbjct: 579 SKDEIHPRGYKCGGNERNLTPEATSEIEREKNESKDISTFNIILDL 624 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,272,811 Number of Sequences: 5004 Number of extensions: 41373 Number of successful extensions: 136 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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