BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060529.seq (670 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_47057| Best HMM Match : Merozoite_SPAM (HMM E-Value=0.94) 29 2.6 SB_15380| Best HMM Match : RA (HMM E-Value=0.11) 29 3.4 SB_28397| Best HMM Match : DUF1605 (HMM E-Value=6.1) 29 4.5 SB_54665| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.0 >SB_47057| Best HMM Match : Merozoite_SPAM (HMM E-Value=0.94) Length = 969 Score = 29.5 bits (63), Expect = 2.6 Identities = 37/133 (27%), Positives = 50/133 (37%) Frame = +2 Query: 155 NHVQNEREKAQE*IS*AERA*E*KKSTYY*LIKSSNSDSEQWVEKESSIVQKETREDWMA 334 N+ NE+ A+E S + A KK Y SS+ + VE+E + W Sbjct: 31 NNNSNEKLTAREGGSGRKNAAN-KKGIYR---TSSHDHVRKLVEQEGRTARNLVT--WSV 84 Query: 335 MTGLLKTYSKQDIKPPRDVKKKNGIDSYNPSTSSRELNPYWKDGGSGLPQTPESFRKADL 514 ++ K PR +K +S RE +P K GGS P S RK L Sbjct: 85 PVSTSQSEEKPKRTKPRVTARKRYSTPDKSGSSEREGSPVTK-GGSKSPNRGRSSRKTSL 143 Query: 515 SSNHLKKMTTIHY 553 SS K Y Sbjct: 144 SSTGRKSDLRARY 156 >SB_15380| Best HMM Match : RA (HMM E-Value=0.11) Length = 2124 Score = 29.1 bits (62), Expect = 3.4 Identities = 21/90 (23%), Positives = 33/90 (36%) Frame = +2 Query: 263 SDSEQWVEKESSIVQKETREDWMAMTGLLKTYSKQDIKPPRDVKKKNGIDSYNPSTSSRE 442 SD E W EKE + + E +E W G + K+ K ++ + + + Sbjct: 1138 SDKEGWKEKEGEVDESE-KEGWKEKEGEVDESEKEGWKEKEGEVDESEKEGWKTEGEGEK 1196 Query: 443 LNPYWKDGGSGLPQTPESFRKADLSSNHLK 532 WK G + E D S +H K Sbjct: 1197 TEEEWKKEVEGQREEKED----DKSDSHTK 1222 >SB_28397| Best HMM Match : DUF1605 (HMM E-Value=6.1) Length = 325 Score = 28.7 bits (61), Expect = 4.5 Identities = 20/70 (28%), Positives = 34/70 (48%), Gaps = 2/70 (2%) Frame = +2 Query: 323 DWMAMTGLLKT--YSKQDIKPPRDVKKKNGIDSYNPSTSSRELNPYWKDGGSGLPQTPES 496 D A GL +T +S +++ +DVK K+G+++ PS + E P W P +S Sbjct: 122 DSAAANGLHETGSFSTPELES-KDVKSKDGLETLIPSKNGGESAPLWSQRQELHPPLSDS 180 Query: 497 FRKADLSSNH 526 +A + H Sbjct: 181 PCQAPRYNGH 190 >SB_54665| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 573 Score = 28.3 bits (60), Expect = 6.0 Identities = 14/47 (29%), Positives = 23/47 (48%) Frame = +2 Query: 284 EKESSIVQKETREDWMAMTGLLKTYSKQDIKPPRDVKKKNGIDSYNP 424 E++ S + ++ ED +K+ K PR KKK G + Y+P Sbjct: 406 ERQGSEIDEDVSEDGEPSQRKRSKGAKKGTKKPRVPKKKEGEEGYDP 452 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,132,278 Number of Sequences: 59808 Number of extensions: 278249 Number of successful extensions: 772 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 723 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 771 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1729817375 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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