Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060527.seq
(687 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p... 140 1e-34
SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces p... 46 7e-06
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 38 0.001
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 27 1.9
SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 1.9
SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 26 4.4
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 26 5.9
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 26 5.9
SPAPJ696.02 |||actin cortical patch component Lsb4 |Schizosaccha... 26 5.9
SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 25 7.8
SPBC725.16 |res1|sct1|MBF transcription factor complex subunit R... 25 7.8
SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm... 25 7.8
>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 525
Score = 140 bits (340), Expect = 1e-34
Identities = 78/138 (56%), Positives = 92/138 (66%), Gaps = 2/138 (1%)
Frame = +3
Query: 279 RLVLEVAQHLGENTVRTIAMDGTEGLVRGQPVLDSGSPIRIPVGAETLGRIINVIGEPID 458
RLVLEVAQH+GENTVRTIAMDGTEGLVRG V+D+GSPI IPVG TLGRI+NVIGEP+D
Sbjct: 91 RLVLEVAQHVGENTVRTIAMDGTEGLVRGTAVIDTGSPISIPVGPGTLGRIMNVIGEPVD 150
Query: 459 ERGPIPTDKTAAIHAEAQSLSTCLCSRRFS*LV*KSSICSLLMP--KEERLGCLAELVWA 632
ERGPI K + IHA+A S + L + LL P + ++G
Sbjct: 151 ERGPIKAVKYSPIHADAPSFEEQSTTPEI--LETGIKVVDLLAPYARGGKIGLFGGAGVG 208
Query: 633 KLY*LWELINNVAKAHGG 686
K + ELINN+AKAHGG
Sbjct: 209 KTVFIQELINNIAKAHGG 226
Score = 25.4 bits (53), Expect = 7.8
Identities = 13/21 (61%), Positives = 15/21 (71%), Gaps = 2/21 (9%)
Frame = +1
Query: 214 DVQFED--NLPPILNALRCKI 270
D QFED +LP ILNAL K+
Sbjct: 66 DCQFEDADSLPSILNALEVKL 86
>SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 536
Score = 45.6 bits (103), Expect = 7e-06
Identities = 25/76 (32%), Positives = 43/76 (56%), Gaps = 1/76 (1%)
Frame = +3
Query: 294 VAQHLGENTVRTIAMDGTEGLVR-GQPVLDSGSPIRIPVGAETLGRIINVIGEPIDERGP 470
+A +L +TV + G + LVR G+ V + + +PVG LGR+++ +G PID +GP
Sbjct: 90 MALNLEADTVGCVLF-GNDRLVREGEVVKRTRHIVDVPVGEALLGRVVDALGNPIDGKGP 148
Query: 471 IPTDKTAAIHAEAQSL 518
I T + + +A +
Sbjct: 149 IKTTERRRVQLKAPGI 164
>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 503
Score = 37.9 bits (84), Expect = 0.001
Identities = 27/63 (42%), Positives = 35/63 (55%), Gaps = 1/63 (1%)
Frame = +3
Query: 285 VLEVAQHLGENTVRTIAMDGTEGL-VRGQPVLDSGSPIRIPVGAETLGRIINVIGEPIDE 461
VLEVA H V +GT G+ VR + +G +RIPV + LGR+ N G PID
Sbjct: 63 VLEVAGHKAIVQV----FEGTSGVDVRKTTIDFTGHSMRIPVSEDMLGRVFNGSGLPID- 117
Query: 462 RGP 470
+GP
Sbjct: 118 KGP 120
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 27.5 bits (58), Expect = 1.9
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = -3
Query: 580 SEQIDDFYTSYENLLLHRHVDKLWASA*IAAVLSVGMGPRS 458
S++ F ++NL H H+++LW + LS+G+G RS
Sbjct: 1020 SQKFHGFLILHDNLKDHIHLEELWLN---LLFLSLGLGIRS 1057
>SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 860
Score = 27.5 bits (58), Expect = 1.9
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -3
Query: 469 GPRSSIGSPITLMMRPRVSA-PTGIRIGEPESSTGCPRTKPSVPSM 335
G R++ G+P + R+++ PT I PES K S PS+
Sbjct: 154 GKRTAPGNPWAIRSAERLASNPTSIGTSSPESIDNNSNNKKSAPSL 199
>SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 732
Score = 26.2 bits (55), Expect = 4.4
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = +2
Query: 95 NPFQNVEKSLQSMPLTRGTMLLNLQEKAKVRLLPLSV 205
NPF N ++S+++MP L+NLQE + + P S+
Sbjct: 115 NPFPNGKQSIKAMP-----SLVNLQEDSVISKFPNSL 146
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.8 bits (54), Expect = 5.9
Identities = 23/80 (28%), Positives = 35/80 (43%), Gaps = 4/80 (5%)
Frame = -2
Query: 617 RQTTQSFLLWHKERADRRLLYQLRESPAAQTCR----QTLGFSMNSSSLVGGDGTTLVNR 450
+ T ++ L K+ D + E+P +T +TL FS++ L D T V
Sbjct: 242 KDTVLAYALSLKQSFDDDRYHDEDETPVTKTISLNDVRTLFFSLSGKLLRLDDVQTPV-L 300
Query: 449 FADYIDDATEGFSSHRDTNW 390
Y DD TE S+ +NW
Sbjct: 301 LLKYADDETEYSSNTAPSNW 320
>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 619
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = +3
Query: 345 TEGLVRGQPVLDSGSPIRIPVGAETLGRIINVIGEPIDE 461
T GL G PV +G P+ + +G I + I P+ +
Sbjct: 77 TSGLTVGDPVQRTGKPLSVELGPGLAETIYDGIQRPLKQ 115
>SPAPJ696.02 |||actin cortical patch component Lsb4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 25.8 bits (54), Expect = 5.9
Identities = 16/54 (29%), Positives = 31/54 (57%), Gaps = 3/54 (5%)
Frame = -3
Query: 541 LLLHRHVDKLWASA*IAAVLSVGMGPRSSIGSPIT---LMMRPRVSAPTGIRIG 389
L++ R D W++ +AV++ GMG + IGS +T +++ + + T R+G
Sbjct: 69 LIVARLDDGTWSAP--SAVMTGGMGVGAQIGSELTDFVIILNSKAAVQTFARLG 120
>SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -3
Query: 409 PTGIRIGEPESSTGCPRTKPSVPSMAMV 326
P G +G PES+ KPS PS + +
Sbjct: 795 PLGHALGNPESNNSSNSFKPSHPSQSFL 822
>SPBC725.16 |res1|sct1|MBF transcription factor complex subunit
Res1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 637
Score = 25.4 bits (53), Expect = 7.8
Identities = 18/68 (26%), Positives = 27/68 (39%)
Frame = -2
Query: 638 QFCPHQFRQTTQSFLLWHKERADRRLLYQLRESPAAQTCRQTLGFSMNSSSLVGGDGTTL 459
Q P+ F Q ++ A+++L Y L QT R+T + D L
Sbjct: 451 QTLPNVFTQLSELSKCHEASLAEKQLTYNLAMEALEQTVRETETCQRLWNERTNNDENYL 510
Query: 458 VNRFADYI 435
VN+ D I
Sbjct: 511 VNQREDLI 518
>SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1292
Score = 25.4 bits (53), Expect = 7.8
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = -3
Query: 541 LLLHRHVDKLWASA*IAAVLSVGMGPRSSIGSPITL 434
L+L DK+ IAAV+S+ +G + G P TL
Sbjct: 228 LMLEAFKDKVLILLSIAAVVSLALGLYQTFGQPPTL 263
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,909,958
Number of Sequences: 5004
Number of extensions: 60760
Number of successful extensions: 207
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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