Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060522.seq
(710 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 98 1e-21
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 82 7e-17
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 73 3e-14
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 72 9e-14
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 69 7e-13
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 68 1e-12
SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 52 6e-08
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 50 2e-07
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 40 5e-04
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 27 2.0
SPBC8D2.16c |||DUF171 family protein|Schizosaccharomyces pombe|c... 25 8.1
>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
Cct2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 97.9 bits (233), Expect = 1e-21
Identities = 68/151 (45%), Positives = 88/151 (58%), Gaps = 2/151 (1%)
Frame = +1
Query: 259 VTNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLH 438
VTNDGATILKSI +DN AAK+LV++SKVQD+EVGDGTTSV V ++ K+H
Sbjct: 59 VTNDGATILKSIALDNAAAKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIH 118
Query: 439 PQTIIAGWRIASDAAKQALAEASL--IIRRT*MRLH*EWI*KTSHGPH*AQRSFSNHKXH 612
PQ II G+RIA+ A AL +S+ R E I +T+ + + S +K H
Sbjct: 119 PQVIIDGYRIATKTAIDALRASSIDNSSDPAKFRSDLENIARTTL----SSKILSQNKNH 174
Query: 613 FTKLAV*WQFCV*KGSXNLKSYPNYPKYLGG 705
F +LAV + KGS NL + K LGG
Sbjct: 175 FAQLAVDAVLRL-KGSTNLDNI-QIIKILGG 203
Score = 63.3 bits (147), Expect = 3e-11
Identities = 31/51 (60%), Positives = 36/51 (70%)
Frame = +2
Query: 80 VSLNPIRILKNXXXXXXXXXXRMSSFIGAIAIGDLVKSTLGPKGMDKILVS 232
+SLNP +I R+SSF+GAIA+GDLVKSTLGPKGMDKIL S
Sbjct: 1 MSLNPHQIFNESGIQERGENARLSSFVGAIAVGDLVKSTLGPKGMDKILQS 51
Score = 37.5 bits (83), Expect = 0.002
Identities = 18/31 (58%), Positives = 24/31 (77%)
Frame = +3
Query: 534 ASLRVDLENIARTTLSSKILFKSQGAFHKIS 626
A R DLENIARTTLSSKIL +++ F +++
Sbjct: 149 AKFRSDLENIARTTLSSKILSQNKNHFAQLA 179
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 82.2 bits (194), Expect = 7e-17
Identities = 37/80 (46%), Positives = 56/80 (70%)
Frame = +1
Query: 232 LREELWSGEVTNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXX 411
L +++ VTNDGATIL + V++PA K+LV++++ QD+EVGDGTTSV +
Sbjct: 50 LVDDIGDVTVTNDGATILSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRA 109
Query: 412 XKLIEQKLHPQTIIAGWRIA 471
+L++ K+HP TII G+R+A
Sbjct: 110 NELVKNKIHPTTIITGYRLA 129
Score = 32.7 bits (71), Expect = 0.053
Identities = 14/29 (48%), Positives = 21/29 (72%)
Frame = +2
Query: 143 RMSSFIGAIAIGDLVKSTLGPKGMDKILV 229
R + + AI ++VKS+LGP G+DK+LV
Sbjct: 23 RNQNVLATTAIANVVKSSLGPVGLDKMLV 51
>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
Cct4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 527
Score = 73.3 bits (172), Expect = 3e-14
Identities = 40/90 (44%), Positives = 54/90 (60%), Gaps = 2/90 (2%)
Frame = +1
Query: 253 GEV--TNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIE 426
GEV TNDGATILK + V +PAAK+LVD+S QD E GDGTTSV + KL++
Sbjct: 53 GEVILTNDGATILKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLK 112
Query: 427 QKLHPQTIIAGWRIASDAAKQALAEASLII 516
+ +HP I ++ A+ + E +L I
Sbjct: 113 KGIHPTVIAESFQRAAGFTVDCMKENALAI 142
Score = 35.1 bits (77), Expect = 0.010
Identities = 12/28 (42%), Positives = 23/28 (82%)
Frame = +2
Query: 143 RMSSFIGAIAIGDLVKSTLGPKGMDKIL 226
R+S+ + A ++ D ++++LGPKGMDK++
Sbjct: 21 RLSNIMAARSVADAIRTSLGPKGMDKMI 48
>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
Cct3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 528
Score = 71.7 bits (168), Expect = 9e-14
Identities = 36/96 (37%), Positives = 58/96 (60%)
Frame = +1
Query: 223 LSFLREELWSGEVTNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXX 402
L L + + S +TNDG IL+ I V +PAAK ++++++ QDEEVGDGTTSV +
Sbjct: 45 LKMLLDPVGSVLLTNDGHAILREIEVAHPAAKSMIELARTQDEEVGDGTTSVIILAGEIL 104
Query: 403 XXXXKLIEQKLHPQTIIAGWRIASDAAKQALAEASL 510
L+++K+HP +I ++ A + A + E +L
Sbjct: 105 AAASPLLDRKIHPVVMIRSFKQALEDALSIIDEITL 140
>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
Cct7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 558
Score = 68.9 bits (161), Expect = 7e-13
Identities = 34/84 (40%), Positives = 50/84 (59%)
Frame = +1
Query: 259 VTNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLH 438
++NDGATI+K + + +PAAK LVD+++ QD EVGDGTTSV V +E +
Sbjct: 62 ISNDGATIMKLLDIVHPAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVEDGVS 121
Query: 439 PQTIIAGWRIASDAAKQALAEASL 510
II G+R A+ A + E ++
Sbjct: 122 SHLIIRGYRKAAQLAVNKIKEIAI 145
Score = 33.9 bits (74), Expect = 0.023
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +2
Query: 92 PIRILKNXXXXXXXXXXRMSSFIGAIAIGDLVKSTLGPKGMDKILV 229
P+ +LK +S+ +A+ D +++TLGP G DK++V
Sbjct: 9 PVIVLKEGTDDSQGRGQLLSNINACVAVQDTIRTTLGPLGADKLMV 54
>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
Cct5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 546
Score = 68.1 bits (159), Expect = 1e-12
Identities = 36/86 (41%), Positives = 50/86 (58%)
Frame = +1
Query: 259 VTNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLH 438
VTNDGATIL + V++ AK+LV +SK QD+E+GDGTT V V LI++ +H
Sbjct: 72 VTNDGATILDQMEVEHQIAKLLVQLSKSQDDEIGDGTTGVVVLAGALLEQAEALIDKGIH 131
Query: 439 PQTIIAGWRIASDAAKQALAEASLII 516
P I G+ A A + L S ++
Sbjct: 132 PIRIADGYEKACQVAVKHLDAISDVV 157
Score = 31.9 bits (69), Expect = 0.093
Identities = 11/28 (39%), Positives = 21/28 (75%)
Frame = +2
Query: 149 SSFIGAIAIGDLVKSTLGPKGMDKILVS 232
S + + ++V+++LGP+G+DKIL+S
Sbjct: 38 SHILATKTVANIVRTSLGPRGLDKILIS 65
>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 546
Score = 52.4 bits (120), Expect = 6e-08
Identities = 27/81 (33%), Positives = 42/81 (51%)
Frame = +1
Query: 259 VTNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLH 438
+TND ATI++ + V +PAAK++VD ++ Q+ E+GD V V +I L
Sbjct: 64 LTNDAATIIRELEVIHPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLT 123
Query: 439 PQTIIAGWRIASDAAKQALAE 501
P I G+ +A + L E
Sbjct: 124 PLEIAKGYEMALSHTMEVLEE 144
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 50.4 bits (115), Expect = 2e-07
Identities = 24/76 (31%), Positives = 37/76 (48%)
Frame = +1
Query: 256 EVTNDGATILKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKL 435
++T DG +L + + NP A + + QD+ GDGTTSV + I + L
Sbjct: 53 KLTKDGKVLLTEMQIQNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREGL 112
Query: 436 HPQTIIAGWRIASDAA 483
HP I G+ +A + A
Sbjct: 113 HPSLISDGFNLAKNEA 128
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 39.5 bits (88), Expect = 5e-04
Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 4/80 (5%)
Frame = +1
Query: 250 SGEVTNDGATILKSIGV----DNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXK 417
S ++T DG T+ +S+ + +N A+++ D++ +E GDGTT+ TV +
Sbjct: 77 SPKITKDGVTVARSVSLKDKFENLGARLVQDVASKTNEVAGDGTTTATVLTRAIFSETVR 136
Query: 418 LIEQKLHPQTIIAGWRIASD 477
+ +P + G ++A D
Sbjct: 137 NVAAGCNPMDLRRGIQLAVD 156
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 27.5 bits (58), Expect = 2.0
Identities = 11/32 (34%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = -3
Query: 321 DFSSRI-IHTDRFQYRSTIICYFA*PEFLPQE 229
D+ +RI + + YR +++C F +FLP+E
Sbjct: 105 DYKTRITLRSPSGSYRGSVVCLFKRSKFLPEE 136
>SPBC8D2.16c |||DUF171 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 315
Score = 25.4 bits (53), Expect = 8.1
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = -3
Query: 294 DRFQYRSTIICYFA*PEFLPQETKILSIPLGPKVLFTKSPMAIAPMKLDIRAT 136
D ++ + ++CYF P F+ +E PL P + +T S + P++ D +T
Sbjct: 80 DPTKFLTDLLCYFETPFFMRKEL----FPLNPHLKYT-SCFPLLPLRNDKAST 127
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,654,705
Number of Sequences: 5004
Number of extensions: 49221
Number of successful extensions: 115
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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