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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060521.seq
         (685 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C...    87   2e-18
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni...    66   6e-12
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit...    58   1e-09
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...    56   6e-09
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ...    54   2e-08
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu...    51   1e-07
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C...    46   7e-06
SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit...    41   2e-04
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60...    30   0.27 

>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
           Cct2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 87.0 bits (206), Expect = 2e-18
 Identities = 50/115 (43%), Positives = 70/115 (60%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           LKSI +DN AAK+LV++SKVQD+EVGDGTTSV V           ++  K+HPQ II G+
Sbjct: 67  LKSIALDNAAAKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIHPQVIIDGY 126

Query: 436 RIASDAAKQALAEASFDHQKNLNEVH*EWI*KTSHGTTLSSKXLFXSQGAFHQIS 600
           RIA+  A  AL  +S D+  +  +   +   +    TTLSSK L  ++  F Q++
Sbjct: 127 RIATKTAIDALRASSIDNSSDPAKFRSDL--ENIARTTLSSKILSQNKNHFAQLA 179



 Score = 77.4 bits (182), Expect = 2e-15
 Identities = 40/67 (59%), Positives = 47/67 (70%)
 Frame = +2

Query: 53  VSLNPIRILKNXXXXXXXXXXRMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVE 232
           +SLNP +I             R+SSF+GAIA+GDLVKSTLGPKGMDKIL S   +SG + 
Sbjct: 1   MSLNPHQIFNESGIQERGENARLSSFVGAIAVGDLVKSTLGPKGMDKILQS--NSSGDIV 58

Query: 233 VTNDGAT 253
           VTNDGAT
Sbjct: 59  VTNDGAT 65



 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 24/44 (54%), Positives = 31/44 (70%)
 Frame = +2

Query: 554 AQRXFSNHKEHFTKLAVDAVLRLKXSGNXKAIQIIQISGGXLEE 685
           + +  S +K HF +LAVDAVLRLK S N   IQII+I GG L++
Sbjct: 164 SSKILSQNKNHFAQLAVDAVLRLKGSTNLDNIQIIKILGGKLDD 207


>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
           Cct1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 65.7 bits (153), Expect = 6e-12
 Identities = 28/63 (44%), Positives = 44/63 (69%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           L  + V++PA K+LV++++ QD+EVGDGTTSV +          +L++ K+HP TII G+
Sbjct: 67  LSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNKIHPTTIITGY 126

Query: 436 RIA 444
           R+A
Sbjct: 127 RLA 129



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 23/46 (50%), Positives = 31/46 (67%)
 Frame = +2

Query: 116 RMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
           R  + +   AI ++VKS+LGP G+DK+LV    + G V VTNDGAT
Sbjct: 23  RNQNVLATTAIANVVKSSLGPVGLDKMLVD---DIGDVTVTNDGAT 65


>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
           Cct3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 528

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 27/73 (36%), Positives = 45/73 (61%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           L+ I V +PAAK ++++++ QDEEVGDGTTSV +           L+++K+HP  +I  +
Sbjct: 65  LREIEVAHPAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLDRKIHPVVMIRSF 124

Query: 436 RIASDAAKQALAE 474
           + A + A   + E
Sbjct: 125 KQALEDALSIIDE 137


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score = 55.6 bits (128), Expect = 6e-09
 Identities = 28/73 (38%), Positives = 41/73 (56%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           LK + V +PAAK+LVD+S  QD E GDGTTSV +          KL+++ +HP  I   +
Sbjct: 65  LKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESF 124

Query: 436 RIASDAAKQALAE 474
           + A+      + E
Sbjct: 125 QRAAGFTVDCMKE 137



 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 21/46 (45%), Positives = 35/46 (76%)
 Frame = +2

Query: 116 RMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
           R+S+ + A ++ D ++++LGPKGMDK ++  G+  G+V +TNDGAT
Sbjct: 21  RLSNIMAARSVADAIRTSLGPKGMDK-MIQTGK--GEVILTNDGAT 63


>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
           Cct7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 558

 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 28/73 (38%), Positives = 40/73 (54%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           +K + + +PAAK LVD+++ QD EVGDGTTSV V            +E  +    II G+
Sbjct: 70  MKLLDIVHPAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVEDGVSSHLIIRGY 129

Query: 436 RIASDAAKQALAE 474
           R A+  A   + E
Sbjct: 130 RKAAQLAVNKIKE 142



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 21/63 (33%), Positives = 36/63 (57%)
 Frame = +2

Query: 65  PIRILKNXXXXXXXXXXRMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTND 244
           P+ +LK            +S+    +A+ D +++TLGP G DK++V    + G+V ++ND
Sbjct: 9   PVIVLKEGTDDSQGRGQLLSNINACVAVQDTIRTTLGPLGADKLMVD---DRGEVVISND 65

Query: 245 GAT 253
           GAT
Sbjct: 66  GAT 68



 Score = 27.9 bits (59), Expect = 1.4
 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = +2

Query: 587 FTKLAVDAVLRLKXSG-NXKAIQIIQISGGXLEE 685
           FTK+ VDAVL L     N   I I ++ GG +E+
Sbjct: 179 FTKMVVDAVLTLDQEDLNENMIGIKKVPGGAMED 212


>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
           Cct5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 546

 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 27/71 (38%), Positives = 39/71 (54%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           L  + V++  AK+LV +SK QD+E+GDGTT V V           LI++ +HP  I  G+
Sbjct: 80  LDQMEVEHQIAKLLVQLSKSQDDEIGDGTTGVVVLAGALLEQAEALIDKGIHPIRIADGY 139

Query: 436 RIASDAAKQAL 468
             A   A + L
Sbjct: 140 EKACQVAVKHL 150



 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 19/44 (43%), Positives = 31/44 (70%)
 Frame = +2

Query: 122 SSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
           S  +    + ++V+++LGP+G+DKIL+S     G++ VTNDGAT
Sbjct: 38  SHILATKTVANIVRTSLGPRGLDKILIS---PDGEITVTNDGAT 78


>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
           Cct6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 535

 Score = 45.6 bits (103), Expect = 7e-06
 Identities = 32/102 (31%), Positives = 46/102 (45%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           L  + + NP A  +   +  QD+  GDGTTSV +            I + LHP  I  G+
Sbjct: 62  LTEMQIQNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREGLHPSLISDGF 121

Query: 436 RIASDAAKQALAEASFDHQKNLNEVH*EWI*KTSHGTTLSSK 561
            +A + A   L     D + +  EV    + KTS  T +SSK
Sbjct: 122 NLAKNEALTFLDSFKTDFEVD-REVLLN-VAKTSLSTKISSK 161


>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
           Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 546

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 22/77 (28%), Positives = 36/77 (46%)
 Frame = +1

Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
           ++ + V +PAAK++VD ++ Q+ E+GD    V V           +I   L P  I  G+
Sbjct: 72  IRELEVIHPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLTPLEIAKGY 131

Query: 436 RIASDAAKQALAEASFD 486
            +A     + L E   D
Sbjct: 132 EMALSHTMEVLEEICAD 148


>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
           Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 582

 Score = 30.3 bits (65), Expect = 0.27
 Identities = 15/59 (25%), Positives = 29/59 (49%)
 Frame = +1

Query: 274 DNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGWRIASD 450
           +N  A+++ D++   +E  GDGTT+ TV          + +    +P  +  G ++A D
Sbjct: 98  ENLGARLVQDVASKTNEVAGDGTTTATVLTRAIFSETVRNVAAGCNPMDLRRGIQLAVD 156



 Score = 26.2 bits (55), Expect = 4.4
 Identities = 15/46 (32%), Positives = 22/46 (47%)
 Frame = +2

Query: 116 RMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
           R S   G   +   V  TLGPKG + ++    +  G  ++T DG T
Sbjct: 44  RASLLTGVDTLARAVSVTLGPKGRNVLI---DQPFGSPKITKDGVT 86


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,379,586
Number of Sequences: 5004
Number of extensions: 41225
Number of successful extensions: 100
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 83
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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