Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060521.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20890.1 68418.m02481 chaperonin, putative similar to SWISS-P... 98 4e-21
At3g20050.1 68416.m02536 T-complex protein 1 alpha subunit / TCP... 67 1e-11
At3g11830.1 68416.m01450 chaperonin, putative similar to SWISS-P... 60 1e-09
At1g24510.1 68414.m03086 T-complex protein 1 epsilon subunit, pu... 57 1e-08
At1g24510.2 68414.m03085 T-complex protein 1 epsilon subunit, pu... 55 5e-08
At5g26360.1 68418.m03151 chaperonin, putative similar to SWISS-P... 53 2e-07
At5g16070.1 68418.m01878 chaperonin, putative similar to SWISS-P... 47 1e-05
At3g02530.1 68416.m00241 chaperonin, putative similar to SWISS-P... 47 1e-05
At3g03960.1 68416.m00415 chaperonin, putative similar to SWISS-P... 46 2e-05
At3g18190.1 68416.m02314 chaperonin, putative similar to SWISS-P... 46 3e-05
At5g56500.1 68418.m07051 chaperonin, putative similar to SWISS-P... 31 0.71
At3g13470.1 68416.m01695 chaperonin, putative similar SWISS-PROT... 31 0.71
At1g55490.2 68414.m06348 RuBisCO subunit binding-protein beta su... 31 0.71
At1g55490.1 68414.m06347 RuBisCO subunit binding-protein beta su... 31 0.71
At3g13860.1 68416.m01751 chaperonin, putative similar to SWISS-P... 30 1.6
At5g10530.1 68418.m01219 lectin protein kinase, putative similar... 29 2.9
At2g33210.1 68415.m04069 chaperonin, putative similar to SWISS-P... 29 2.9
At3g23990.1 68416.m03013 chaperonin (CPN60) (HSP60) identical to... 29 3.8
At4g02920.2 68417.m00396 expressed protein 27 8.8
At4g02920.1 68417.m00395 expressed protein 27 8.8
>At5g20890.1 68418.m02481 chaperonin, putative similar to
SWISS-PROT:P78371- T-complex protein 1, beta subunit
(TCP-1-beta) [Homo sapiens]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 527
Score = 98.3 bits (234), Expect = 4e-21
Identities = 53/115 (46%), Positives = 75/115 (65%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
LKS+ +DNPAAK+LVD+SKVQD+EVGDGTTSV V KL+ K+HP TIIAG+
Sbjct: 66 LKSLHIDNPAAKVLVDISKVQDDEVGDGTTSVVVLAGELLREAEKLVASKIHPMTIIAGY 125
Query: 436 RIASDAAKQALAEASFDHQKNLNEVH*EWI*KTSHGTTLSSKXLFXSQGAFHQIS 600
R+AS+ A+ AL + D++ N + + + K + TTL SK L + F +++
Sbjct: 126 RMASECARNALLKRVIDNKDNAEKFRSDLL-KIAM-TTLCSKILSQDKEHFAEMA 178
Score = 70.1 bits (164), Expect = 1e-12
Identities = 37/61 (60%), Positives = 42/61 (68%)
Frame = +2
Query: 71 RILKNXXXXXXXXXXRMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGA 250
+I K+ RM+SF+GA+AI DLVKSTLGPKGMDKIL S GR V VTNDGA
Sbjct: 5 KIFKDDASEEKGERARMASFVGAMAISDLVKSTLGPKGMDKILQSTGRGHA-VTVTNDGA 63
Query: 251 T 253
T
Sbjct: 64 T 64
Score = 48.4 bits (110), Expect = 4e-06
Identities = 23/42 (54%), Positives = 30/42 (71%)
Frame = +2
Query: 560 RXFSNHKEHFTKLAVDAVLRLKXSGNXKAIQIIQISGGXLEE 685
+ S KEHF ++AVDAV RLK S N +AIQII+ GG L++
Sbjct: 165 KILSQDKEHFAEMAVDAVFRLKGSTNLEAIQIIKKPGGSLKD 206
>At3g20050.1 68416.m02536 T-complex protein 1 alpha subunit /
TCP-1-alpha / chaperonin (CCT1) identical to
SWISS-PROT:P28769- T-complex protein 1, alpha subunit
(TCP-1-alpha) [Arabidopsis thaliana]
Length = 545
Score = 66.9 bits (156), Expect = 1e-11
Identities = 31/85 (36%), Positives = 53/85 (62%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
L+ + V++PAAK+LV+++++QD EVGDGTTSV + L+ K+HP +II+G+
Sbjct: 65 LRMLEVEHPAAKVLVELAELQDREVGDGTTSVVIVAAELLKRANDLVRNKIHPTSIISGY 124
Query: 436 RIASDAAKQALAEASFDHQKNLNEV 510
R+A + + + E + L +V
Sbjct: 125 RLAMRESCKYIEEKLVTKVEKLGKV 149
Score = 41.9 bits (94), Expect = 4e-04
Identities = 20/46 (43%), Positives = 31/46 (67%)
Frame = +2
Query: 116 RMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
R + + A+ ++VK++LGP G+DK+LV + G V +TNDGAT
Sbjct: 21 RTQNVMACQAVSNIVKTSLGPVGLDKMLVD---DIGDVTITNDGAT 63
>At3g11830.1 68416.m01450 chaperonin, putative similar to
SWISS-PROT:P80313 T-complex protein 1, eta subunit
(TCP-1-eta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 557
Score = 60.1 bits (139), Expect = 1e-09
Identities = 38/104 (36%), Positives = 55/104 (52%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
+K + + +PAAKILVD++K QD EVGDGTT+V + IE +H Q +I +
Sbjct: 69 MKLLDIVHPAAKILVDIAKSQDSEVGDGTTTVVLLAAEFLKEAKPFIEDGVHAQNLIRSY 128
Query: 436 RIASDAAKQALAEASFDHQKNLNEVH*EWI*KTSHGTTLSSKXL 567
R AS A + E + + E + K + TTLSSK +
Sbjct: 129 RTASTLAIAKVKELAVSIEGKSVEEKKGLLAKCA-ATTLSSKLI 171
Score = 45.2 bits (102), Expect = 4e-05
Identities = 20/45 (44%), Positives = 32/45 (71%)
Frame = +2
Query: 119 MSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
+S+ A+GD+V++TLGP+GMDK++ + G V ++NDGAT
Sbjct: 26 VSNINACTAVGDVVRTTLGPRGMDKLIHD---DKGSVTISNDGAT 67
>At1g24510.1 68414.m03086 T-complex protein 1 epsilon subunit,
putative / TCP-1-epsilon, putative / chaperonin,
putative identical to SWISS-PROT:O04450- T-complex
protein 1, epsilon subunit (TCP-1-epsilon) [Arabidopsis
thaliana]; strong similarity to SP|P54411 T-complex
protein 1, epsilon subunit (TCP-1-epsilon) (CCT-epsilon)
(TCP-K36) {Avena sativa}; contains Pfam:PF00118 domain,
TCP-1/cpn60 chaperonin family
Length = 535
Score = 56.8 bits (131), Expect = 1e-08
Identities = 35/102 (34%), Positives = 57/102 (55%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
L+ + VDN AK++V++S+ QD E+GDGTT V V + +++ +HP I G+
Sbjct: 74 LEQMDVDNQIAKLMVELSRSQDYEIGDGTTGVVVMAGALLEQAERQLDRGIHPIRIAEGY 133
Query: 436 RIASDAAKQALAEASFDHQKNLNEVH*EWI*KTSHGTTLSSK 561
+AS A + L + + ++N E + +T TTLSSK
Sbjct: 134 EMASRVAVEHLERIAQKFEFDVNNY--EPLVQTCM-TTLSSK 172
Score = 39.5 bits (88), Expect = 0.002
Identities = 18/37 (48%), Positives = 26/37 (70%)
Frame = +2
Query: 143 AIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
A+ +++S+LGPKGMDK+L G + +TNDGAT
Sbjct: 39 AVARILRSSLGPKGMDKMLQG---PDGDITITNDGAT 72
>At1g24510.2 68414.m03085 T-complex protein 1 epsilon subunit,
putative / TCP-1-epsilon, putative / chaperonin,
putative identical to SWISS-PROT:O04450- T-complex
protein 1, epsilon subunit (TCP-1-epsilon) [Arabidopsis
thaliana]; strong similarity to SP|P54411 T-complex
protein 1, epsilon subunit (TCP-1-epsilon) (CCT-epsilon)
(TCP-K36) {Avena sativa}; contains Pfam:PF00118 domain,
TCP-1/cpn60 chaperonin family
Length = 459
Score = 54.8 bits (126), Expect = 5e-08
Identities = 34/97 (35%), Positives = 54/97 (55%)
Frame = +1
Query: 271 VDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGWRIASD 450
VDN AK++V++S+ QD E+GDGTT V V + +++ +HP I G+ +AS
Sbjct: 3 VDNQIAKLMVELSRSQDYEIGDGTTGVVVMAGALLEQAERQLDRGIHPIRIAEGYEMASR 62
Query: 451 AAKQALAEASFDHQKNLNEVH*EWI*KTSHGTTLSSK 561
A + L + + ++N E + +T TTLSSK
Sbjct: 63 VAVEHLERIAQKFEFDVNNY--EPLVQTCM-TTLSSK 96
>At5g26360.1 68418.m03151 chaperonin, putative similar to
SWISS-PROT:P50143- T-complex protein 1, gamma subunit
(TCP-1-gamma) [Xenopus laevis]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 555
Score = 52.8 bits (121), Expect = 2e-07
Identities = 24/56 (42%), Positives = 34/56 (60%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTI 423
L+ + V +PAAK ++++S+ QDEEVGDGTTSV V +E+ HP I
Sbjct: 65 LRELDVAHPAAKSMIELSRTQDEEVGDGTTSVIVLAGEMLHVAEAFLEKNYHPTVI 120
>At5g16070.1 68418.m01878 chaperonin, putative similar to
SWISS-PROT:P80317 T-complex protein 1, zeta subunit
(TCP-1-zeta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 535
Score = 47.2 bits (107), Expect = 1e-05
Identities = 22/71 (30%), Positives = 37/71 (52%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
LK + + NP A ++ + QD+ GDGTTS + + I++ +HP+ ++ G+
Sbjct: 63 LKEMQIQNPTAIMIARTAVAQDDISGDGTTSTVIFIGELMKQSERCIDEGMHPRVLVDGF 122
Query: 436 RIASDAAKQAL 468
IA A Q L
Sbjct: 123 EIAKRATLQFL 133
>At3g02530.1 68416.m00241 chaperonin, putative similar to
SWISS-PROT:P80317- T-complex protein 1, zeta subunit
(TCP-1-zeta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 535
Score = 47.2 bits (107), Expect = 1e-05
Identities = 22/71 (30%), Positives = 37/71 (52%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
LK + + NP A ++ + QD+ GDGTTS + + I++ +HP+ ++ G+
Sbjct: 63 LKEMQIQNPTAIMIARTAVAQDDISGDGTTSTVIFIGELMKQSERCIDEGMHPRVLVDGF 122
Query: 436 RIASDAAKQAL 468
IA A Q L
Sbjct: 123 EIAKRATLQFL 133
>At3g03960.1 68416.m00415 chaperonin, putative similar to
SWISS-PROT:P42932- T-complex protein 1, theta subunit
(TCP-1-theta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 549
Score = 46.0 bits (104), Expect = 2e-05
Identities = 24/73 (32%), Positives = 36/73 (49%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTIIAGW 435
+ + + +PAAK+LV +K Q EE+GDG +LI LHP II+G+
Sbjct: 73 VNELEIQHPAAKLLVLAAKAQQEEIGDGANLTISFAGELLQNAEELIRMGLHPSEIISGY 132
Query: 436 RIASDAAKQALAE 474
A A + L +
Sbjct: 133 TKAVSKAVEILEQ 145
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/36 (33%), Positives = 25/36 (69%)
Frame = +2
Query: 146 IGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
+ + +++LGP GM+K++++ + ++ VTND AT
Sbjct: 39 LSTITRTSLGPNGMNKMVIN---HLDKLFVTNDAAT 71
>At3g18190.1 68416.m02314 chaperonin, putative similar to
SWISS-PROT:P50991- T-complex protein 1, delta subunit
(TCP-1-delta) [Homo sapiens]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 536
Score = 45.6 bits (103), Expect = 3e-05
Identities = 23/56 (41%), Positives = 30/56 (53%)
Frame = +1
Query: 256 LKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVTVXXXXXXXXXXKLIEQKLHPQTI 423
L + V PAAK+LV++SK QD GDGTT+V V L+ +HP I
Sbjct: 74 LNKMEVLQPAAKMLVELSKSQDSAAGDGTTTVVVIAGALLKECQSLLTNGIHPTVI 129
Score = 44.8 bits (101), Expect = 5e-05
Identities = 21/46 (45%), Positives = 33/46 (71%)
Frame = +2
Query: 116 RMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
R ++ A A+ D V+++LGPKGMDK++ + +G+V +TNDGAT
Sbjct: 30 RFANINSARAVSDAVRTSLGPKGMDKMISTA---NGEVIITNDGAT 72
>At5g56500.1 68418.m07051 chaperonin, putative similar to
SWISS-PROT:P08927- RuBisCO subunit binding-protein beta
subunit, chloroplast precursor (60 kDa chaperonin beta
subunit, CPN-60 beta) [Pisum sativum]; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 597
Score = 31.1 bits (67), Expect = 0.71
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +2
Query: 134 GAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
G + DLV TLGPKG + +L S G + NDG T
Sbjct: 70 GVNKLADLVGVTLGPKGRNVVLES---KYGSPRIVNDGVT 106
>At3g13470.1 68416.m01695 chaperonin, putative similar
SWISS-PROT:P21240- RuBisCO subunit binding-protein beta
subunit, chloroplast precursor (60 kDa chaperonin beta
subunit, CPN-60 beta) [Arabidopsis thaliana]; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 596
Score = 31.1 bits (67), Expect = 0.71
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +2
Query: 134 GAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
G + DLV TLGPKG + +L S G + NDG T
Sbjct: 70 GVNKLADLVGVTLGPKGRNVVLES---KYGSPRIVNDGVT 106
>At1g55490.2 68414.m06348 RuBisCO subunit binding-protein beta
subunit, chloroplast / 60 kDa chaperonin beta subunit /
CPN-60 beta identical to SWISS-PROT:P21240- RuBisCO
subunit binding-protein beta subunit, chloroplast
precursor (60 kDa chaperonin beta subunit, CPN-60 beta)
[Arabidopsis thaliana]
Length = 600
Score = 31.1 bits (67), Expect = 0.71
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +2
Query: 134 GAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
G + DLV TLGPKG + +L S G + NDG T
Sbjct: 74 GVNKLADLVGVTLGPKGRNVVLES---KYGSPRIVNDGVT 110
>At1g55490.1 68414.m06347 RuBisCO subunit binding-protein beta
subunit, chloroplast / 60 kDa chaperonin beta subunit /
CPN-60 beta identical to SWISS-PROT:P21240- RuBisCO
subunit binding-protein beta subunit, chloroplast
precursor (60 kDa chaperonin beta subunit, CPN-60 beta)
[Arabidopsis thaliana]
Length = 600
Score = 31.1 bits (67), Expect = 0.71
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +2
Query: 134 GAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
G + DLV TLGPKG + +L S G + NDG T
Sbjct: 74 GVNKLADLVGVTLGPKGRNVVLES---KYGSPRIVNDGVT 110
>At3g13860.1 68416.m01751 chaperonin, putative similar to
SWISS-PROT:P29197- chaperonin CPN60, mitochondrial
precursor (HSP60) [Arabidopsis thaliana] ; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 572
Score = 29.9 bits (64), Expect = 1.6
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +2
Query: 116 RMSSFIGAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
R + G + + VK T+GPKG + I+ S + G ++T DG T
Sbjct: 43 RAAMLQGVSEVAEAVKVTMGPKGRNVIIES---SYGGPKITKDGVT 85
>At5g10530.1 68418.m01219 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 651
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = +1
Query: 244 WCYELKSIGVDNPAAKILVDMSKVQDEEVGDGTTSVT 354
W Y+L S ++N + ++D+SKV EV G ++ +
Sbjct: 200 WTYDLTSDPLENSSLSYIIDLSKVLPSEVTIGFSATS 236
>At2g33210.1 68415.m04069 chaperonin, putative similar to
SWISS-PROT:Q05046- chaperonin CPN60-2, mitochondrial
precursor (HSP60-2) [Cucurbita maxima]; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 585
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +2
Query: 134 GAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
G + D VK T+GPKG + I+ ++ G +VT DG T
Sbjct: 50 GVEDLADAVKVTMGPKGRNVII---EQSWGAPKVTKDGVT 86
>At3g23990.1 68416.m03013 chaperonin (CPN60) (HSP60) identical to
SWISS-PROT:P29197- chaperonin CPN60, mitochondrial
precursor (HSP60) [Arabidopsis thaliana]
Length = 577
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +2
Query: 134 GAIAIGDLVKSTLGPKGMDKILVSCGRNSGQVEVTNDGAT 253
G + D VK T+GPKG + ++ ++ G +VT DG T
Sbjct: 49 GVEDLADAVKVTMGPKGRNVVI---EQSWGAPKVTKDGVT 85
>At4g02920.2 68417.m00396 expressed protein
Length = 419
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -3
Query: 128 NWTSEQLQLSPLLLHF*GYELDLMIPFWMK 39
N T++ QLSPL+ HF +E D+ P +K
Sbjct: 211 NSTTKWRQLSPLIPHFQRFESDVFTPAKLK 240
>At4g02920.1 68417.m00395 expressed protein
Length = 418
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -3
Query: 128 NWTSEQLQLSPLLLHF*GYELDLMIPFWMK 39
N T++ QLSPL+ HF +E D+ P +K
Sbjct: 210 NSTTKWRQLSPLIPHFQRFESDVFTPAKLK 239
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,282,087
Number of Sequences: 28952
Number of extensions: 210832
Number of successful extensions: 389
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 368
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 385
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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