Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060517.seq
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g17070.1 68415.m01971 expressed protein contains Pfam profile... 35 0.054
At5g38130.1 68418.m04594 transferase family protein similar to a... 31 0.67
At2g33490.1 68415.m04105 hydroxyproline-rich glycoprotein family... 31 0.67
At1g46768.1 68414.m05217 AP2 domain-containing protein RAP2.1 (R... 31 0.88
At3g42690.1 68416.m04439 Ulp1 protease family protein contains P... 29 2.7
At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing ... 29 3.6
At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing ... 29 3.6
At5g20750.1 68418.m02466 Ulp1 protease family protein contains P... 29 3.6
At4g04130.1 68417.m00583 Ulp1 protease family protein contains P... 29 3.6
At1g51140.1 68414.m05749 basic helix-loop-helix (bHLH) family pr... 29 3.6
At1g29670.1 68414.m03626 GDSL-motif lipase/hydrolase family prot... 29 3.6
At5g46750.1 68418.m05759 human Rev interacting-like family prote... 28 4.7
At1g68820.1 68414.m07868 membrane protein, putative contains 7 t... 28 4.7
At3g18215.1 68416.m02317 expressed protein contains Pfam profile... 28 6.2
At3g17770.1 68416.m02267 dihydroxyacetone kinase family protein ... 27 8.2
At1g43870.1 68414.m05054 hypothetical protein similar to fructos... 27 8.2
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 27 8.2
>At2g17070.1 68415.m01971 expressed protein contains Pfam profile
PF03087: Arabidopsis protein of unknown function;
expression supported by MPSS
Length = 263
Score = 34.7 bits (76), Expect = 0.054
Identities = 19/55 (34%), Positives = 31/55 (56%), Gaps = 1/55 (1%)
Frame = -2
Query: 162 NASRDAARLKRSAYVAWDNARRRRDPNISGERRKYNAASRSYKKVIAKA-KSARA 1
N S+DA + + + RR+ ++SGE +KY A+ +S+KK K KS +A
Sbjct: 95 NISKDALSQMKEGLMEIQSILRRKRGDLSGEVKKYLASRKSFKKTFQKVQKSLKA 149
>At5g38130.1 68418.m04594 transferase family protein similar to
anthranilate N-hydroxycinnamoyl/benzoyltransferase,
Dianthus caryophyllus [gi:2239091]; contains Pfam
transferase family domain PF002458
Length = 462
Score = 31.1 bits (67), Expect = 0.67
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = -3
Query: 356 WRYMSADWDGLREFYASYPWGRFCFSSADPDVCATVLKTWCSRG 225
+ +M AD +F+ + W + C + +DP + + VLK W G
Sbjct: 183 YNHMVADGTCFWKFF--HTWSKICLNGSDPSIQSIVLKDWFCDG 224
>At2g33490.1 68415.m04105 hydroxyproline-rich glycoprotein family
protein Common family member:At3g26910 [Arabidopsis
thaliana]
Length = 623
Score = 31.1 bits (67), Expect = 0.67
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = -2
Query: 654 KTLTLTTKSGWGPEPLTSRVGLPTISPWPTASPSW*HSPPVSQI 523
K L+ T+ GP P LP +S PTASP++ +P +S++
Sbjct: 467 KPLSTTSHLYSGPIPRNPVSKLPKVSSSPTASPTFVSTPKISEL 510
>At1g46768.1 68414.m05217 AP2 domain-containing protein RAP2.1
(RAP2.1) identical to AP2 domain containing protein
RAP2.1 GI:2281627 from [Arabidopsis thaliana]
Length = 153
Score = 30.7 bits (66), Expect = 0.88
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -1
Query: 646 NAHHQEWLGSRTTDLPGRTAYDFALAY 566
N + WLGS TTD+ AYD A+ Y
Sbjct: 50 NKRSRLWLGSYTTDIAAARAYDVAVFY 76
>At3g42690.1 68416.m04439 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At2g11345, At4g04130
Length = 1009
Score = 29.1 bits (62), Expect = 2.7
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -3
Query: 320 EFYASYPWGRFCFSS 276
EF+ SYPWGR F S
Sbjct: 149 EFFMSYPWGRVAFES 163
>At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 420
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 228 PGAPRL*DGRTDVRISRGKAEPPP 299
P PR+ DG + RGKA PPP
Sbjct: 388 PPGPRMPDGTRGFTMGRGKAIPPP 411
>At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 422
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 228 PGAPRL*DGRTDVRISRGKAEPPP 299
P PR+ DG + RGKA PPP
Sbjct: 390 PPGPRMPDGTRGFTMGRGKAIPPP 413
>At5g20750.1 68418.m02466 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 944
Score = 28.7 bits (61), Expect = 3.6
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -3
Query: 320 EFYASYPWGRFCFSS 276
EF+ +YPWGR FSS
Sbjct: 223 EFFMAYPWGREAFSS 237
>At4g04130.1 68417.m00583 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At2g11345, At3g42690
Length = 1200
Score = 28.7 bits (61), Expect = 3.6
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -3
Query: 320 EFYASYPWGRFCFSS 276
EF+ SYPWGR F S
Sbjct: 284 EFFLSYPWGRVSFES 298
>At1g51140.1 68414.m05749 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 379
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = +2
Query: 203 EEGINNSIPWSTTSLR--RSHRRQDQQRKSRTAPMGRMRKIHAVHPNLLTYT 352
E+ +++SIP + R +H R +R RT RMRK+ + PN+ T T
Sbjct: 293 EQLLSDSIPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDTQT 344
>At1g29670.1 68414.m03626 GDSL-motif lipase/hydrolase family protein
similar to family II lipase EXL1 GI:15054382 from
[Arabidopsis thaliana]; contains Pfam profile: PF00657
Lipase/Acylhydrolase with GDSL-like motif
Length = 363
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -2
Query: 132 RSAYVAWDNARRRRDPNISGERRKYNAASRS 40
R+AYV WD N+ RR YNA S S
Sbjct: 320 RNAYVFWDAFHPTEAANVIIARRSYNAQSAS 350
>At5g46750.1 68418.m05759 human Rev interacting-like family protein
/ hRIP family protein contains Pfam profile PF01412:
Putative GTP-ase activating protein for Arf
Length = 402
Score = 28.3 bits (60), Expect = 4.7
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 325 SPSQSADIYRQTLRYPVVVRRSGRESGTAAR 417
SP S + T + P+V R+SG+ G AR
Sbjct: 169 SPKASQKVVASTFKKPLVSRKSGKTGGLGAR 199
>At1g68820.1 68414.m07868 membrane protein, putative contains 7
transmembrane domains; similar to inhibitor of
apoptosis-2 IAP-2 (GI:20043383) [Mamestra configurata
nucleopolyhedrovirus]
Length = 468
Score = 28.3 bits (60), Expect = 4.7
Identities = 27/83 (32%), Positives = 40/83 (48%), Gaps = 1/83 (1%)
Frame = +3
Query: 252 GRTDVRISRGKAEPPPWVGCVKFT-QSIPIC*HIPPNSSIPGRRSTIRTREWYGSTNKAV 428
G D+ I+ G AE ++ C K++ QSI HIP SS +R +W N+ +
Sbjct: 181 GWWDLFINFGIAECFAFLVCTKWSNQSIHRYSHIPEPSS---SSMVVRYLDW----NRGL 233
Query: 429 IRRSKRSVDHHTVSGRICGQQKV 497
+ + D H S RICG Q +
Sbjct: 234 VVTAD---DEHQQSNRICGLQDI 253
>At3g18215.1 68416.m02317 expressed protein contains Pfam profile
PF04654: Protein of unknown function, DUF599
Length = 244
Score = 27.9 bits (59), Expect = 6.2
Identities = 19/64 (29%), Positives = 25/64 (39%), Gaps = 1/64 (1%)
Frame = -3
Query: 428 HCLIRAAVPLSRPDRRTTTGYRRVWRYMSADWD-GLREFYASYPWGRFCFSSADPDVCAT 252
H VP+SR R R S W GLR FY S+P + F VC
Sbjct: 143 HVSFLVTVPVSRGKREHCEYVSRNLNRASYFWSLGLRAFYFSFPLFLWTFGPIPMFVCCC 202
Query: 251 VLKT 240
++ +
Sbjct: 203 MMSS 206
>At3g17770.1 68416.m02267 dihydroxyacetone kinase family protein
contains Pfam domains, PF02733: DAK1 domain and PF02734:
DAK2 domain
Length = 595
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = -2
Query: 144 ARLKRSAYVAWDNARRRRDPNISGERRKYNAASRSYKK 31
A+ RS+YV+ +N DP YNAA+R+ K+
Sbjct: 552 AQAGRSSYVSAENLATVPDPGAMAAAGWYNAAARAVKE 589
>At1g43870.1 68414.m05054 hypothetical protein similar to fructose
binding protein FrcB (GI:11066207)[Sinorhizobium
meliloti]
Length = 295
Score = 27.5 bits (58), Expect = 8.2
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -1
Query: 457 WSTLRLDRLITALFVLP 407
WSTLR DR++ AL + P
Sbjct: 263 WSTLRRDRIVAALAIAP 279
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 27.5 bits (58), Expect = 8.2
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +2
Query: 572 QGEIVGSPTREVSGSGPQPLLVVSVK 649
Q +I+ P + VSG G +P++VVS K
Sbjct: 86 QSDILHWPFKVVSGPGEKPMIVVSYK 111
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,131,967
Number of Sequences: 28952
Number of extensions: 426073
Number of successful extensions: 1401
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1334
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1398
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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