BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060509.seq (685 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19F8.01c |spn7|SPBC21.08c|septin Spn7|Schizosaccharomyces po... 31 0.20 SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 29 0.47 SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit ... 27 2.5 SPBC365.07c |||TATA element modulatory factor homolog |Schizosac... 27 3.3 SPBC106.17c |cys2||O-acetyltransferase |Schizosaccharomyces pomb... 27 3.3 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 25 7.7 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 25 7.7 SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|... 25 7.7 SPBC947.05c |||ferric-chelate reductase |Schizosaccharomyces pom... 25 7.7 SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 25 7.7 >SPBC19F8.01c |spn7|SPBC21.08c|septin Spn7|Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 30.7 bits (66), Expect = 0.20 Identities = 22/69 (31%), Positives = 31/69 (44%) Frame = +1 Query: 229 KREQTALDKHTTTQLCKQKNSGVNIEEKHDQAIVNHEKNVGFDSSIGDHEPRPNTSYLYS 408 + E+ + D + T L QK + + E D+ VN + SSI E R N S Sbjct: 284 RTEKLSNDSPSNTSLSLQKQNSIVANE--DKRSVNGSERTETRSSIDQSEMRTNVSDSTK 341 Query: 409 RADLKAINS 435 +LK INS Sbjct: 342 SEELKKINS 350 >SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 629 Score = 29.5 bits (63), Expect = 0.47 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%) Frame = -2 Query: 342 LLMVYNSLIVFFFNVHSAIFLFTQLCGC-VFIKSGLFSFPKFSNLLFMACTLFFGCDIII 166 ++ VY L +N F+ CG VFI S + F K S+ L+FG ++I Sbjct: 540 IITVYFQLCSENYNWWWRSFITPGFCGIYVFIFSVFYWFFKISSSSLATAVLYFGYSLLI 599 Query: 165 STLL 154 S L+ Sbjct: 600 SVLV 603 >SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit Sfc4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1006 Score = 27.1 bits (57), Expect = 2.5 Identities = 22/98 (22%), Positives = 43/98 (43%) Frame = +1 Query: 214 IRELRKREQTALDKHTTTQLCKQKNSGVNIEEKHDQAIVNHEKNVGFDSSIGDHEPRPNT 393 I EL+ AL+ T + +Q+ + +N E+ +HEKNVG +G+ + P Sbjct: 509 INELQDNRDAALE--IVTNIFEQRRN-INELEREQSQNEDHEKNVGSQLFVGNQKV-PQD 564 Query: 394 SYLYSRADLKAINSDNKTCLSRQTSDPGKKLHRSHAFR 507 + RA + + + +T + ++ H+ R Sbjct: 565 KW-EKRARISRSKEEARQFTIWKTEETQRRFHKLDILR 601 >SPBC365.07c |||TATA element modulatory factor homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 26.6 bits (56), Expect = 3.3 Identities = 17/49 (34%), Positives = 24/49 (48%) Frame = +3 Query: 507 IDRNQIAPKRHGSCNGRSETANFTMISNQKLSKERLKILGNFLEXQMKK 653 +D NQI G+ + E + Q LSK LK L N ++ Q+KK Sbjct: 25 LDGNQIEEMSRGNAKSKDEIIAKLLTEGQALSKNELK-LNNTIK-QLKK 71 >SPBC106.17c |cys2||O-acetyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 504 Score = 26.6 bits (56), Expect = 3.3 Identities = 21/77 (27%), Positives = 37/77 (48%) Frame = +1 Query: 193 STSHKE*IRELRKREQTALDKHTTTQLCKQKNSGVNIEEKHDQAIVNHEKNVGFDSSIGD 372 S SH+ + E RK+ Q LDK+ + + ++ +N E+ V +++ D + Sbjct: 359 SASHQRSLSENRKKNQHKLDKYLSADVSAEEIIKLN-EDTSVLPDVPYQEIANEDRA--- 414 Query: 373 HEPRPNTSYLYSRADLK 423 EP P T+ + A LK Sbjct: 415 PEPDPETNLIAGLAPLK 431 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 25.4 bits (53), Expect = 7.7 Identities = 17/49 (34%), Positives = 25/49 (51%) Frame = +3 Query: 483 VAQESCF*IDRNQIAPKRHGSCNGRSETANFTMISNQKLSKERLKILGN 629 VA+ES + +N A C G T +++ QK K+RLK +GN Sbjct: 591 VARESISALRKNVTA-----KCYGGDVTRKQKLLNKQKEGKKRLKSVGN 634 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.4 bits (53), Expect = 7.7 Identities = 11/18 (61%), Positives = 15/18 (83%) Frame = -3 Query: 236 SRFLSSRIYSLWLVLCFL 183 SR LSS ++SL L+LC+L Sbjct: 563 SRGLSSTVWSLQLLLCYL 580 >SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1078 Score = 25.4 bits (53), Expect = 7.7 Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 4/50 (8%) Frame = +1 Query: 139 YLKCAQKCRNNDIAAKKQSTSH--KE*IRELRK--REQTALDKHTTTQLC 276 ++K AQKCR + +A + T E IR L K + T HT + C Sbjct: 589 FIKIAQKCRRHFVAQQLGETEPFINEIIRNLAKTTEDLTPQQTHTFYEAC 638 >SPBC947.05c |||ferric-chelate reductase |Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.4 bits (53), Expect = 7.7 Identities = 10/35 (28%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Frame = -1 Query: 493 SCATFFLGHWFDVINRFYCHC*WPS-DQLCYINSW 392 SC FF+ ++F + N +C + S +++ Y++ W Sbjct: 118 SCGLFFVSYFFSLKNNPFCLMLFSSHEKMNYLHRW 152 >SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 453 Score = 25.4 bits (53), Expect = 7.7 Identities = 12/44 (27%), Positives = 17/44 (38%) Frame = +1 Query: 367 GDHEPRPNTSYLYSRADLKAINSDNKTCLSRQTSDPGKKLHRSH 498 G + P NTSY +N N+ C + G + R H Sbjct: 303 GFYPPPENTSYFVLNLSNTTLNVKNELCFPIPSGKKGAPVFRKH 346 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,654,056 Number of Sequences: 5004 Number of extensions: 54111 Number of successful extensions: 153 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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