Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060506.seq
(686 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_O60832 Cluster: H/ACA ribonucleoprotein complex subunit... 78 2e-13
UniRef50_O13473 Cluster: Centromere/microtubule-binding protein ... 76 9e-13
UniRef50_Q10KT9 Cluster: H/ACA ribonucleoprotein complex subunit... 69 1e-10
UniRef50_Q7QR24 Cluster: GLP_576_9913_8648; n=1; Giardia lamblia... 61 3e-08
UniRef50_Q2R1N7 Cluster: Kinetochore protein, putative; n=1; Ory... 51 2e-05
UniRef50_Q0JAU9 Cluster: Os04g0576400 protein; n=1; Oryza sativa... 46 0.001
UniRef50_Q18GM5 Cluster: TRNA-pseudouridine synthase; n=1; Haloq... 39 0.099
UniRef50_Q41BZ2 Cluster: TRNA pseudouridine synthase B; n=1; Exi... 38 0.17
UniRef50_UPI00015BB216 Cluster: putative rRNA pseudouridine synt... 38 0.23
UniRef50_Q8PV20 Cluster: Probable tRNA pseudouridine synthase B;... 38 0.30
UniRef50_A3CMF2 Cluster: TRNA pseudouridine synthase B, putative... 36 0.70
UniRef50_Q57612 Cluster: Probable tRNA pseudouridine synthase B;... 36 0.93
UniRef50_Q5UXH1 Cluster: Probable tRNA pseudouridine synthase B;... 36 0.93
UniRef50_P60346 Cluster: Probable tRNA pseudouridine synthase B;... 36 1.2
UniRef50_Q190V0 Cluster: TRNA pseudouridine synthase B; n=2; Des... 34 2.8
UniRef50_Q8Y7F3 Cluster: tRNA pseudouridine synthase B; n=31; Ba... 34 2.8
UniRef50_Q9YDC3 Cluster: Probable tRNA pseudouridine synthase B;... 34 2.8
UniRef50_Q9RUQ9 Cluster: tRNA pseudouridine synthase B; n=2; Dei... 34 3.7
UniRef50_Q18NU4 Cluster: Cbf5; n=3; Sulfolobaceae|Rep: Cbf5 - Me... 33 4.9
UniRef50_Q88VL5 Cluster: tRNA pseudouridine synthase B; n=4; Lac... 33 6.5
UniRef50_Q5NER4 Cluster: tRNA pseudouridine synthase B; n=11; Fr... 33 6.5
UniRef50_Q3ABA1 Cluster: tRNA pseudouridine synthase B; n=1; Car... 33 6.5
UniRef50_Q0AYI5 Cluster: Pseudouridylate synthase; n=1; Syntroph... 33 8.6
>UniRef50_O60832 Cluster: H/ACA ribonucleoprotein complex subunit 4;
n=78; Eukaryota|Rep: H/ACA ribonucleoprotein complex
subunit 4 - Homo sapiens (Human)
Length = 514
Score = 77.8 bits (183), Expect = 2e-13
Identities = 39/76 (51%), Positives = 48/76 (63%)
Frame = +1
Query: 388 LKRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDX 567
LKR + DY+++GFIN+DK S + + K GTLD KV GC VCI+
Sbjct: 79 LKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIER 138
Query: 568 ATRLVKSQQNAGKEYV 615
ATRLVKSQQ+AGKEYV
Sbjct: 139 ATRLVKSQQSAGKEYV 154
Score = 64.1 bits (149), Expect = 3e-09
Identities = 28/45 (62%), Positives = 32/45 (71%)
Frame = +3
Query: 258 FKIEPSEXVKKLDXAYWXLLLKNFDRLNVRTNHYTPLPFGNXPFK 392
F I+P V KLD + W LLLKNFD+LNVRT HYTPL G+ P K
Sbjct: 36 FLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLK 80
>UniRef50_O13473 Cluster: Centromere/microtubule-binding protein
CBF5; n=54; cellular organisms|Rep:
Centromere/microtubule-binding protein CBF5 -
Kluyveromyces lactis (Yeast) (Candida sphaerica)
Length = 474
Score = 75.8 bits (178), Expect = 9e-13
Identities = 39/76 (51%), Positives = 44/76 (57%)
Frame = +1
Query: 388 LKRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDX 567
LKR + Y+ SG IN+DK S K + K GTLD KV GC VC+D
Sbjct: 48 LKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCEKTGHSGTLDPKVTGCLIVCVDR 107
Query: 568 ATRLVKSQQNAGKEYV 615
ATRLVKSQQ AGKEYV
Sbjct: 108 ATRLVKSQQGAGKEYV 123
Score = 47.6 bits (108), Expect = 3e-04
Identities = 20/45 (44%), Positives = 28/45 (62%)
Frame = +3
Query: 258 FKIEPSEXVKKLDXAYWXLLLKNFDRLNVRTNHYTPLPFGNXPFK 392
F I+P + + W LLLK++D+L VR+ HYTP+P G P K
Sbjct: 5 FVIKPESVSPSSNTSEWPLLLKDYDKLLVRSGHYTPIPAGASPLK 49
>UniRef50_Q10KT9 Cluster: H/ACA ribonucleoprotein complex subunit 4,
putative, expressed; n=8; Oryza sativa|Rep: H/ACA
ribonucleoprotein complex subunit 4, putative, expressed
- Oryza sativa subsp. japonica (Rice)
Length = 591
Score = 68.5 bits (160), Expect = 1e-10
Identities = 37/76 (48%), Positives = 44/76 (57%)
Frame = +1
Query: 388 LKRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDX 567
LKR + +Y++ G IN+DK S K + K GTLD KV G VC+D
Sbjct: 97 LKRPIAEYLRYGVINLDKPSNPSSHEVVAWIKRLLRVEKTGHSGTLDPKVTGNLIVCVDR 156
Query: 568 ATRLVKSQQNAGKEYV 615
ATRLVKSQQ AGKEYV
Sbjct: 157 ATRLVKSQQGAGKEYV 172
Score = 61.7 bits (143), Expect = 2e-08
Identities = 26/45 (57%), Positives = 31/45 (68%)
Frame = +3
Query: 258 FKIEPSEXVKKLDXAYWXLLLKNFDRLNVRTNHYTPLPFGNXPFK 392
+ I+P LD + W LLLKN+DRLNVRT HYTPLP G+ P K
Sbjct: 54 YLIKPQSVAPPLDTSAWPLLLKNYDRLNVRTGHYTPLPAGHSPLK 98
>UniRef50_Q7QR24 Cluster: GLP_576_9913_8648; n=1; Giardia lamblia
ATCC 50803|Rep: GLP_576_9913_8648 - Giardia lamblia ATCC
50803
Length = 421
Score = 60.9 bits (141), Expect = 3e-08
Identities = 33/76 (43%), Positives = 43/76 (56%)
Frame = +1
Query: 388 LKRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDX 567
L+R + +++K G IN+DK + + + K GTLD V GC VC+D
Sbjct: 39 LQRPIREHLKYGVINLDKTAGPSSHEVVAWIRRILEVEKTGHSGTLDPGVTGCLLVCLDR 98
Query: 568 ATRLVKSQQNAGKEYV 615
ATRLVKSQQ GKEYV
Sbjct: 99 ATRLVKSQQLMGKEYV 114
>UniRef50_Q2R1N7 Cluster: Kinetochore protein, putative; n=1; Oryza
sativa (japonica cultivar-group)|Rep: Kinetochore
protein, putative - Oryza sativa subsp. japonica (Rice)
Length = 142
Score = 51.2 bits (117), Expect = 2e-05
Identities = 24/32 (75%), Positives = 25/32 (78%)
Frame = +1
Query: 517 GTLDRKVXGCXXVCIDXATRLVKSQQNAGKEY 612
GTLD KV G VC+D ATRLVKSQQ AGKEY
Sbjct: 76 GTLDPKVTGNLIVCVDLATRLVKSQQGAGKEY 107
>UniRef50_Q0JAU9 Cluster: Os04g0576400 protein; n=1; Oryza sativa
(japonica cultivar-group)|Rep: Os04g0576400 protein -
Oryza sativa subsp. japonica (Rice)
Length = 97
Score = 45.6 bits (103), Expect = 0.001
Identities = 22/33 (66%), Positives = 23/33 (69%)
Frame = -1
Query: 614 TYSLPAFCCDLTSLVAXSMQTIXQPXTFRSSVP 516
TYSLPA CCDLT VA S QT+ P T SSVP
Sbjct: 29 TYSLPAPCCDLTRRVARSTQTMRLPVTLGSSVP 61
>UniRef50_Q18GM5 Cluster: TRNA-pseudouridine synthase; n=1;
Haloquadratum walsbyi DSM 16790|Rep: TRNA-pseudouridine
synthase - Haloquadratum walsbyi (strain DSM 16790)
Length = 339
Score = 39.1 bits (87), Expect = 0.099
Identities = 23/75 (30%), Positives = 32/75 (42%)
Frame = +1
Query: 394 RXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXAT 573
R + D G +N+DK + S + + GTLD KV GC + + AT
Sbjct: 14 RSLADICNFGVVNLDKPVGPSAHQVSAWVRDLIGVERAAHAGTLDPKVTGCLPILLGEAT 73
Query: 574 RLVKSQQNAGKEYVA 618
RL KEY+A
Sbjct: 74 RLAPVFLEGTKEYLA 88
>UniRef50_Q41BZ2 Cluster: TRNA pseudouridine synthase B; n=1;
Exiguobacterium sibiricum 255-15|Rep: TRNA pseudouridine
synthase B - Exiguobacterium sibiricum 255-15
Length = 302
Score = 38.3 bits (85), Expect = 0.17
Identities = 21/66 (31%), Positives = 33/66 (50%)
Frame = +1
Query: 421 GFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXATRLVKSQQNA 600
G + +DK + F + F++ K GTLD +V G +C+ AT+L + +
Sbjct: 5 GVLPLDKPAGMTSHDCVFRLRRLFQTKKVGHTGTLDPEVTGVLPICLGRATKLARFITDE 64
Query: 601 GKEYVA 618
GK YVA
Sbjct: 65 GKRYVA 70
>UniRef50_UPI00015BB216 Cluster: putative rRNA pseudouridine
synthase; n=1; Ignicoccus hospitalis KIN4/I|Rep:
putative rRNA pseudouridine synthase - Ignicoccus
hospitalis KIN4/I
Length = 349
Score = 37.9 bits (84), Expect = 0.23
Identities = 24/75 (32%), Positives = 35/75 (46%)
Frame = +1
Query: 391 KRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXA 570
KR + + V+ G +N+DK K F + GTLD KV G V + A
Sbjct: 39 KRDVKELVELGVVNLDKPPGPTSHEVVAWIKRMFGLERAGHGGTLDPKVTGVLPVALAEA 98
Query: 571 TRLVKSQQNAGKEYV 615
T+++ +GKEYV
Sbjct: 99 TKVIHYVMLSGKEYV 113
>UniRef50_Q8PV20 Cluster: Probable tRNA pseudouridine synthase B;
n=6; Euryarchaeota|Rep: Probable tRNA pseudouridine
synthase B - Methanosarcina mazei (Methanosarcina
frisia)
Length = 338
Score = 37.5 bits (83), Expect = 0.30
Identities = 21/75 (28%), Positives = 34/75 (45%)
Frame = +1
Query: 391 KRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXA 570
KR + +Y++ G +N+DK + K G+LD KV G + A
Sbjct: 33 KRPIHEYIEKGVVNIDKPRGPTSHEVAAWVKAILGVHTAGHAGSLDPKVTGLLPTLLGKA 92
Query: 571 TRLVKSQQNAGKEYV 615
T V + + +GKEY+
Sbjct: 93 TLAVPALRLSGKEYI 107
>UniRef50_A3CMF2 Cluster: TRNA pseudouridine synthase B, putative;
n=7; Streptococcus|Rep: TRNA pseudouridine synthase B,
putative - Streptococcus sanguinis (strain SK36)
Length = 292
Score = 36.3 bits (80), Expect = 0.70
Identities = 22/65 (33%), Positives = 31/65 (47%)
Frame = +1
Query: 418 SGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXATRLVKSQQN 597
+G IN+ K + + F + K+ K GTLD V G + + ATRLV+ Q
Sbjct: 2 NGIINLRKEAGMTSHDAVFKLRKILKTKKIGHGGTLDPDVVGVLPIAVGKATRLVEFMQE 61
Query: 598 AGKEY 612
GK Y
Sbjct: 62 EGKVY 66
>UniRef50_Q57612 Cluster: Probable tRNA pseudouridine synthase B;
n=9; Euryarchaeota|Rep: Probable tRNA pseudouridine
synthase B - Methanococcus jannaschii
Length = 336
Score = 35.9 bits (79), Expect = 0.93
Identities = 24/75 (32%), Positives = 32/75 (42%)
Frame = +1
Query: 391 KRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXA 570
+R + D +K G + VDK S K K GTLD KV G V ++ A
Sbjct: 36 ERKIEDLIKYGVVVVDKPRGPTSHEVSTWVKKILNLDKAGHGGTLDPKVTGVLPVALERA 95
Query: 571 TRLVKSQQNAGKEYV 615
T+ + KEYV
Sbjct: 96 TKTIPMWHIPPKEYV 110
>UniRef50_Q5UXH1 Cluster: Probable tRNA pseudouridine synthase B;
n=3; Halobacteriaceae|Rep: Probable tRNA pseudouridine
synthase B - Haloarcula marismortui (Halobacterium
marismortui)
Length = 314
Score = 35.9 bits (79), Expect = 0.93
Identities = 21/65 (32%), Positives = 30/65 (46%)
Frame = +1
Query: 421 GFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXATRLVKSQQNA 600
G +N+DK + + + + GTLD KV GC V + A R+ + NA
Sbjct: 37 GVVNLDKPPGPSAHQVAAWIRDATGQDRVAHGGTLDPKVTGCLPVLLGDAARMAQVFDNA 96
Query: 601 GKEYV 615
KEYV
Sbjct: 97 VKEYV 101
>UniRef50_P60346 Cluster: Probable tRNA pseudouridine synthase B;
n=12; Archaea|Rep: Probable tRNA pseudouridine synthase
B - Nanoarchaeum equitans
Length = 348
Score = 35.5 bits (78), Expect = 1.2
Identities = 17/34 (50%), Positives = 23/34 (67%)
Frame = +1
Query: 517 GTLDRKVXGCXXVCIDXATRLVKSQQNAGKEYVA 618
GTLD KV G + I AT+++++ AGKEYVA
Sbjct: 90 GTLDPKVTGVLPIAIGEATKVLQTLLIAGKEYVA 123
>UniRef50_Q190V0 Cluster: TRNA pseudouridine synthase B; n=2;
Desulfitobacterium hafniense|Rep: TRNA pseudouridine
synthase B - Desulfitobacterium hafniense (strain DCB-2)
Length = 294
Score = 34.3 bits (75), Expect = 2.8
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = +1
Query: 517 GTLDRKVXGCXXVCIDXATRLVKSQQNAGKEY 612
GTLD +V G +C+ ATRL + N GK Y
Sbjct: 35 GTLDPEVTGVLPICVGKATRLAEYITNQGKSY 66
>UniRef50_Q8Y7F3 Cluster: tRNA pseudouridine synthase B; n=31;
Bacillales|Rep: tRNA pseudouridine synthase B - Listeria
monocytogenes
Length = 304
Score = 34.3 bits (75), Expect = 2.8
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +1
Query: 517 GTLDRKVXGCXXVCIDXATRLVKSQQNAGKEYVA 618
GTLD +V G +CI AT+L + + GK YVA
Sbjct: 35 GTLDPEVEGVLPICIGRATKLAEYVTDEGKVYVA 68
>UniRef50_Q9YDC3 Cluster: Probable tRNA pseudouridine synthase B;
n=24; Thermoprotei|Rep: Probable tRNA pseudouridine
synthase B - Aeropyrum pernix
Length = 354
Score = 34.3 bits (75), Expect = 2.8
Identities = 22/75 (29%), Positives = 34/75 (45%)
Frame = +1
Query: 391 KRXMXDYVKSGFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXA 570
+R + Y++ G I VDK K + GTLD KV G V ++
Sbjct: 47 ERPLDVYLRYGMIVVDKPPGPTSHEVVAWIKRMLGVSRAGHGGTLDPKVTGVLPVALERM 106
Query: 571 TRLVKSQQNAGKEYV 615
TR++ + ++ KEYV
Sbjct: 107 TRIIGTVMHSSKEYV 121
>UniRef50_Q9RUQ9 Cluster: tRNA pseudouridine synthase B; n=2;
Deinococcus|Rep: tRNA pseudouridine synthase B -
Deinococcus radiodurans
Length = 308
Score = 33.9 bits (74), Expect = 3.7
Identities = 14/35 (40%), Positives = 23/35 (65%)
Frame = +1
Query: 517 GTLDRKVXGCXXVCIDXATRLVKSQQNAGKEYVAF 621
GTLD G +C+D +T+LV+ ++ KEY+A+
Sbjct: 34 GTLDPLATGVVVLCVDDSTKLVQFMEHDTKEYLAW 68
>UniRef50_Q18NU4 Cluster: Cbf5; n=3; Sulfolobaceae|Rep: Cbf5 -
Metallosphaera sedula
Length = 73
Score = 33.5 bits (73), Expect = 4.9
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = +1
Query: 469 SFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXATRLVKSQQNAGKEYV 615
+F KT F + GTLD KV G + + AT+++ +GKEYV
Sbjct: 1 AFWVKTMFNLPRVGHGGTLDPKVTGVLPIGLGKATKIMSVVTKSGKEYV 49
>UniRef50_Q88VL5 Cluster: tRNA pseudouridine synthase B; n=4;
Lactobacillus|Rep: tRNA pseudouridine synthase B -
Lactobacillus plantarum
Length = 307
Score = 33.1 bits (72), Expect = 6.5
Identities = 15/32 (46%), Positives = 20/32 (62%)
Frame = +1
Query: 517 GTLDRKVXGCXXVCIDXATRLVKSQQNAGKEY 612
GTLD V G +CI AT++V+ +GKEY
Sbjct: 36 GTLDPNVDGVLPICIGNATKVVQFLVASGKEY 67
>UniRef50_Q5NER4 Cluster: tRNA pseudouridine synthase B; n=11;
Francisella tularensis|Rep: tRNA pseudouridine synthase
B - Francisella tularensis subsp. tularensis
Length = 302
Score = 33.1 bits (72), Expect = 6.5
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +1
Query: 481 KTYFKSXKDCXLGTLDRKVXGCXXVCIDXATRLVKSQQNAGKEYVA 618
K F + K GTLD G +C AT++ + +A KEY+A
Sbjct: 30 KYLFNAQKAGHTGTLDPMATGVLPICFGRATKIAQYLLDADKEYIA 75
>UniRef50_Q3ABA1 Cluster: tRNA pseudouridine synthase B; n=1;
Carboxydothermus hydrogenoformans Z-2901|Rep: tRNA
pseudouridine synthase B - Carboxydothermus
hydrogenoformans (strain Z-2901 / DSM 6008)
Length = 288
Score = 33.1 bits (72), Expect = 6.5
Identities = 24/67 (35%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 421 GFINVDKXSKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXATRLVKSQQN- 597
GFIN+ K + K+ K LGTLD G V + ATRL++ +N
Sbjct: 3 GFINLLKPPGMTSHDVVAYVRKKLKTGKVGHLGTLDPAAAGVLPVAVGQATRLIEYLENV 62
Query: 598 AGKEYVA 618
A KEY+A
Sbjct: 63 AMKEYLA 69
>UniRef50_Q0AYI5 Cluster: Pseudouridylate synthase; n=1;
Syntrophomonas wolfei subsp. wolfei str. Goettingen|Rep:
Pseudouridylate synthase - Syntrophomonas wolfei subsp.
wolfei (strain Goettingen)
Length = 299
Score = 32.7 bits (71), Expect = 8.6
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
Frame = +1
Query: 421 GFINVDKX-SKXQLXRSSFMDKTYFKSXKDCXLGTLDRKVXGCXXVCIDXATRLVKSQQN 597
GF+N++K + + K + K LGTLD G V + TR++ ++
Sbjct: 3 GFLNINKPQAMTSFDVIKKLKKVLPRKYKLGHLGTLDPMAEGVLPVAVGCGTRIIPFVED 62
Query: 598 AGKEYVAFST 627
KEY+A T
Sbjct: 63 ETKEYIATMT 72
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 492,091,423
Number of Sequences: 1657284
Number of extensions: 6657481
Number of successful extensions: 11164
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 10917
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 11146
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53719013270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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