Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060501.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g38070.1 68417.m05377 bHLH family protein contains Pfam profi... 35 0.058
At1g06530.1 68414.m00692 myosin heavy chain-related similar to m... 34 0.076
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 33 0.18
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 33 0.18
At2g46180.1 68415.m05742 intracellular protein transport protein... 30 1.6
At1g34320.1 68414.m04259 expressed protein contains Pfam domain ... 30 1.6
At1g19230.1 68414.m02393 respiratory burst oxidase protein E (Rb... 30 1.6
At5g48310.1 68418.m05968 expressed protein 29 2.2
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 29 2.2
At2g38720.1 68415.m04755 microtubule associated protein (MAP65/A... 29 2.2
At1g44880.1 68414.m05142 Ulp1 protease family protein similar to... 29 2.9
At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative (CH... 28 5.0
At1g33970.1 68414.m04212 avirulence-responsive protein, putative... 28 6.6
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 28 6.6
At5g46860.1 68418.m05774 syntaxin 22 (SYP22) (VAM3) identical to... 27 8.8
At5g25500.1 68418.m03034 expressed protein ; expression supporte... 27 8.8
At3g49010.2 68416.m05354 60S ribosomal protein L13 (RPL13B) / br... 27 8.8
At3g49010.1 68416.m05353 60S ribosomal protein L13 (RPL13B) / br... 27 8.8
At1g02330.1 68414.m00178 expressed protein contains similarity t... 27 8.8
>At4g38070.1 68417.m05377 bHLH family protein contains Pfam profile:
PF00010 helix-loop-helix DNA-binding domain; PMID:
12679534; putative bHLH131 transcription factor
Length = 1513
Score = 34.7 bits (76), Expect = 0.058
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = +1
Query: 325 RAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKL 432
+ EW E +++ DEI +L+T L S R S DL++KL
Sbjct: 211 KKEWEEEKSKLLDEIYSLQTKLDSVTRISEDLQKKL 246
>At1g06530.1 68414.m00692 myosin heavy chain-related similar to
myosin heavy chain (GI:1408194) {Placopecten
magellanicus}; similar to Myosin heavy chain, clone 203
(Fragment) (SP:P39922){Hydra attenuata}; contains one
transmembrane domain
Length = 323
Score = 34.3 bits (75), Expect = 0.076
Identities = 19/54 (35%), Positives = 34/54 (62%), Gaps = 3/54 (5%)
Frame = +1
Query: 310 QADQLRAEWSRE---LARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITE 462
+A++LR+E S++ + +E E+A LRTV + ++ +L+ KLG KE+ E
Sbjct: 131 EAEKLRSEISQKGGGIEELEKEVAGLRTVKEENEKRMKELESKLGALEVKELDE 184
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 33.1 bits (72), Expect = 0.18
Identities = 34/118 (28%), Positives = 51/118 (43%), Gaps = 2/118 (1%)
Frame = +1
Query: 307 EQADQLRAEWSRELARVEDEIATLRTVLQSK--IRQSSDLKRKLGITVWKEITEDVNQGL 480
E AD+L E RE++R+ DE+ LR ++SK +S G TE Q +
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESKPVWNKSPSQVHHYG----NNNTESQQQDV 640
Query: 481 KNVKESQVYHR*RIAWLLLQMAVTETPIYQKTESVIKTTAEKTSSIHRRHHGRRVQQA 654
N+ S H +I L Q A E + Q T+ I E+ + R + Q+A
Sbjct: 641 SNLSTSAAEH--QILILARQQAQREEELAQ-TQRHILALQEEIEELERENRLHSQQEA 695
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 33.1 bits (72), Expect = 0.18
Identities = 34/118 (28%), Positives = 51/118 (43%), Gaps = 2/118 (1%)
Frame = +1
Query: 307 EQADQLRAEWSRELARVEDEIATLRTVLQSK--IRQSSDLKRKLGITVWKEITEDVNQGL 480
E AD+L E RE++R+ DE+ LR ++SK +S G TE Q +
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESKPVWNKSPSQVHHYG----NNNTESQQQDV 640
Query: 481 KNVKESQVYHR*RIAWLLLQMAVTETPIYQKTESVIKTTAEKTSSIHRRHHGRRVQQA 654
N+ S H +I L Q A E + Q T+ I E+ + R + Q+A
Sbjct: 641 SNLSTSAAEH--QILILARQQAQREEELAQ-TQRHILALQEEIEELERENRLHSQQEA 695
>At2g46180.1 68415.m05742 intracellular protein transport protein
USO1-related similar to Intracellular protein transport
protein USO1 (Swiss-Prot:P25386) [Saccharomyces
cerevisiae]
Length = 725
Score = 29.9 bits (64), Expect = 1.6
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +1
Query: 313 ADQLRAEWSRELARVEDEIATLRTVLQSKI 402
A+ + AEW +++VED+ A +R VL+ +
Sbjct: 528 AENIAAEWKNRVSKVEDDNAKVRRVLEQSM 557
>At1g34320.1 68414.m04259 expressed protein contains Pfam domain
PF05003: protein of unknown function (DUF668)
Length = 657
Score = 29.9 bits (64), Expect = 1.6
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = +1
Query: 355 VEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNV 489
V D +A LRT L+S+ + +LK+K ++W I E+V + L +V
Sbjct: 308 VGDTLAILRTELKSQKKHVRNLKKK---SLWSRILEEVMEKLVDV 349
>At1g19230.1 68414.m02393 respiratory burst oxidase protein E
(RbohE) / NADPH oxidase nearly identical to respiratory
burst oxidase protein E GI:3242787 [gi:3242787] from
[Arabidopsis thaliana]
Length = 926
Score = 29.9 bits (64), Expect = 1.6
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = +3
Query: 177 HSFHTVRVFHCKI*AVFKQLKKQCQARSGRGHT 275
H TV VF+C I V K+LKKQ Q S + T
Sbjct: 885 HPNSTVGVFYCGIQTVAKELKKQAQDMSQKTTT 917
>At5g48310.1 68418.m05968 expressed protein
Length = 1156
Score = 29.5 bits (63), Expect = 2.2
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 3/56 (5%)
Frame = +1
Query: 268 DIHTPDELAGLTPEQADQLRAEWSRELARVEDEI---ATLRTVLQSKIRQSSDLKR 426
DI TP + Q D + E +++ ++EDEI A + + Q +R SS L R
Sbjct: 159 DIRTPSAPPIMESGQEDSISLEIEKDIQKIEDEICGEAGVESSKQESMRSSSHLYR 214
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 29.5 bits (63), Expect = 2.2
Identities = 19/60 (31%), Positives = 31/60 (51%)
Frame = +1
Query: 319 QLRAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKES 498
Q+R+E ELA I L TV+ ++ ++ L KL +T D+N+ L VK++
Sbjct: 2415 QMRSEKDEELAAARLRIEELETVVSTRQKEIFLLNSKLAKV--DSMTHDINRVLLGVKQN 2472
>At2g38720.1 68415.m04755 microtubule associated protein
(MAP65/ASE1) family protein low similarity to myosin
[Schistosoma japonicum] GI:3941320; contains Pfam
profile PF03999: Microtubule associated protein
(MAP65/ASE1 family)
Length = 587
Score = 29.5 bits (63), Expect = 2.2
Identities = 19/56 (33%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +1
Query: 307 EQADQLRAEWSRELARVEDEIATLRTVLQSKI--RQSSDLKRKLGITVWKEITEDV 468
E+ + RAE R LA+ E EIA+L + L K+ + + K I+ K + ED+
Sbjct: 50 EKTRKFRAELQRSLAQAEAEIASLMSALGEKVSFAKKKEGSLKEQISSVKPVLEDL 105
>At1g44880.1 68414.m05142 Ulp1 protease family protein similar to
At5g28170, At1g35110, At3g42530, At4g19320, At5g36020,
At4g03970, At3g43010, At2g10350; contains Pfam profile
PF02902: Ulp1 protease family, C-terminal catalytic
domain
Length = 1038
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = +1
Query: 487 VKESQVYHR*RIAWLLLQMAVTETPIYQKTESVIKTTAEKTSSIHRRH 630
VK+ +V H + W Q T+ P T I +K S +H++H
Sbjct: 343 VKDKKVAHMYNLIWSSHQFQKTDWPGGDATLPPISVPKKKNSGVHKKH 390
>At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative
(CHX16) monovalent cation:proton antiporter family 2
(CPA2) member, PMID:11500563
Length = 847
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 433 QVYVLSPSFD-GSCFAVQFSKSRFHLRHGLARDSIQRVTG 317
++ V PS G ++ +F+K L++ L RDS+Q +TG
Sbjct: 71 EIIVSPPSTGLGQSYSFRFNKYPTRLKYELYRDSVQLITG 110
>At1g33970.1 68414.m04212 avirulence-responsive protein, putative /
avirulence induced gene protein, putative / AIG protein,
putative similar to SP|P54120 AIG1 protein {Arabidopsis
thaliana}; contains Pfam profile PF04548: AIG1 family
Length = 342
Score = 27.9 bits (59), Expect = 6.6
Identities = 19/48 (39%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
Frame = +1
Query: 298 LTPEQADQLRAEW-SRELA-RVEDEIATLRTVLQSKIRQSSDLKRKLG 435
L EQA +L AE + E+ R DEI LR L+ +++ +L++KLG
Sbjct: 290 LGEEQAARLEAEKRANEVQKRSSDEIKKLRENLERAEKETKELQKKLG 337
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = -3
Query: 317 SACSGVRPANSSGVCMSPATPCLTLLLQLFEHRSYFT 207
S+CS V+ NS SP+ +L F HR Y T
Sbjct: 5 SSCSSVKAVNSRWTSPSPSPSSRFAVLPAFLHRRYAT 41
>At5g46860.1 68418.m05774 syntaxin 22 (SYP22) (VAM3) identical to
GP|8809669| syntaxin related protein AtVam3p
[Arabidopsis thaliana]
Length = 268
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 3/62 (4%)
Frame = +1
Query: 304 PEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGIT--VWKEITEDVN-Q 474
PEQ QL+ +EL +++EIA V++ + + ++ +++G ++K++ VN Q
Sbjct: 150 PEQRAQLQESKRQELVLLDNEIAFNEAVIEEREQGIQEIHQQIGEVNEIFKDLAVLVNDQ 209
Query: 475 GL 480
G+
Sbjct: 210 GV 211
>At5g25500.1 68418.m03034 expressed protein ; expression supported
by MPSS
Length = 420
Score = 27.5 bits (58), Expect = 8.8
Identities = 19/75 (25%), Positives = 34/75 (45%)
Frame = +1
Query: 391 QSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYHR*RIAWLLLQMAVTETPIYQ 570
Q K+ +S+ + +W+ ED+ GLK + V HR +L V + +
Sbjct: 149 QRKLVRSTVCSQVTQYRIWRGTLEDILNGLKEEVDWLVEHREMSQGRVLAQQVILSCLRF 208
Query: 571 KTESVIKTTAEKTSS 615
+ES + EK++S
Sbjct: 209 LSESSVSFEVEKSTS 223
>At3g49010.2 68416.m05354 60S ribosomal protein L13 (RPL13B) /
breast basic conserved protein 1-related (BBC1)
Length = 206
Score = 27.5 bits (58), Expect = 8.8
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +1
Query: 262 AGDIHTPDELAGLTPEQADQL---RAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKL 432
AGD TP+ELA T Q D L R + + EL ++ E+ + + KIR KR
Sbjct: 134 AGD-STPEELANATQVQGDYLPIVREKPTMELVKLTSEMKSFKAF--DKIRLERTNKRHA 190
Query: 433 G 435
G
Sbjct: 191 G 191
>At3g49010.1 68416.m05353 60S ribosomal protein L13 (RPL13B) /
breast basic conserved protein 1-related (BBC1)
Length = 206
Score = 27.5 bits (58), Expect = 8.8
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +1
Query: 262 AGDIHTPDELAGLTPEQADQL---RAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKL 432
AGD TP+ELA T Q D L R + + EL ++ E+ + + KIR KR
Sbjct: 134 AGD-STPEELANATQVQGDYLPIVREKPTMELVKLTSEMKSFKAF--DKIRLERTNKRHA 190
Query: 433 G 435
G
Sbjct: 191 G 191
>At1g02330.1 68414.m00178 expressed protein contains similarity to
hepatocellular carcinoma-associated antigen 59
GI:7158847 from [Homo sapiens]
Length = 279
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/57 (31%), Positives = 30/57 (52%)
Frame = +1
Query: 331 EWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQ 501
E EL RVEDE+ + L+ K ++SS+ T E+ + LKN++E++
Sbjct: 122 EVENELKRVEDELYKIPDHLKVK-KRSSEESSTQWTTGIAEVQLPIEYKLKNIEETE 177
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,084,741
Number of Sequences: 28952
Number of extensions: 286397
Number of successful extensions: 755
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 736
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 755
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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