BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060500.seq (503 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPJ696.02 |||actin cortical patch component Lsb4 |Schizosaccha... 58 1e-09 SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces po... 54 2e-08 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 52 5e-08 SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 50 2e-07 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 47 1e-06 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 47 1e-06 SPBC11C11.02 |imp2||contractile ring protein Imp2|Schizosaccharo... 47 2e-06 SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 46 4e-06 SPAC7D4.02c |||src |Schizosaccharomyces pombe|chr 1|||Manual 40 3e-04 SPBC19C2.10 |||BAR adaptor protein|Schizosaccharomyces pombe|chr... 40 3e-04 SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyce... 38 6e-04 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 38 9e-04 SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces pom... 38 9e-04 SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces ... 37 0.002 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 32 0.056 SPCC24B10.13 |skb5||Shk1 kinase binding protein 5|Schizosaccharo... 29 0.30 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.30 SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 29 0.40 SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schi... 28 0.69 SPBC16H5.11c |skb1|rmt5|type II protein arginine N-methyltransfe... 28 0.92 SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 1.6 SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 26 2.8 SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 26 3.7 SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 26 3.7 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 6.5 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 25 6.5 SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 25 8.5 SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces p... 25 8.5 >SPAPJ696.02 |||actin cortical patch component Lsb4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 57.6 bits (133), Expect = 1e-09 Identities = 22/51 (43%), Positives = 34/51 (66%), Gaps = 2/51 (3%) Frame = +1 Query: 310 QALYDFTPQEAXELEFRRGDVITVTDRSDQH--WWQGXIAHRRGLFPAXYV 456 +A+Y F ++ +L F++GD+I + +RS H WW G I +R G+FPA YV Sbjct: 377 RAMYSFAGEQPGDLSFQKGDIIDIVERSGSHDDWWTGRIGYREGIFPANYV 427 >SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces pombe|chr 2|||Manual Length = 373 Score = 53.6 bits (123), Expect = 2e-08 Identities = 22/50 (44%), Positives = 31/50 (62%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRRGLFPAXYV 456 V+ALYDF E EL F++GD+I V + + WW+G + G+FP YV Sbjct: 220 VRALYDFAATEQGELSFKKGDIILVLESVYKDWWKGSCKNAVGIFPVNYV 269 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 52.0 bits (119), Expect = 5e-08 Identities = 22/50 (44%), Positives = 30/50 (60%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRRGLFPAXYV 456 V+A+YD+ QE EL F ++I D D +WW+G RGLFP+ YV Sbjct: 805 VKAIYDYQAQEDNELSFFEDEIIANVDCVDPNWWEGECHGHRGLFPSNYV 854 Score = 42.3 bits (95), Expect = 4e-05 Identities = 17/50 (34%), Positives = 30/50 (60%), Gaps = 1/50 (2%) Frame = +1 Query: 316 LYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAH-RRGLFPAXYVTV 462 +YD++P+E E+E + I + + D WW G + ++GLFP+ YV + Sbjct: 733 IYDYSPEEENEIELVENEQIQILEFVDDGWWLGENSKGQQGLFPSNYVEI 782 >SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 296 Score = 50.4 bits (115), Expect = 2e-07 Identities = 20/50 (40%), Positives = 31/50 (62%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRRGLFPAXYV 456 V A+YDF +A +L F G+VI V + + WW+G + + G+FP+ YV Sbjct: 145 VLAMYDFPGPDAGDLGFHAGEVIIVLEHVNNDWWRGELNGKEGIFPSNYV 194 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 47.2 bits (107), Expect = 1e-06 Identities = 23/59 (38%), Positives = 33/59 (55%), Gaps = 4/59 (6%) Frame = +1 Query: 292 PIEMLVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAH----RRGLFPAXYV 456 PI V ALYD+ Q E+ F++GD + V + WW G I + +RGLFP+ +V Sbjct: 866 PILGYVIALYDYQAQIPEEISFQKGDTLMVLRTQEDGWWDGEIINVPNSKRGLFPSNFV 924 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 47.2 bits (107), Expect = 1e-06 Identities = 23/52 (44%), Positives = 28/52 (53%), Gaps = 2/52 (3%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSD--QHWWQGXIAHRRGLFPAXYV 456 V ALYD+ Q A +L F GD I V R+D WW G + +G FP YV Sbjct: 412 VVALYDYAAQAAGDLSFHAGDRIEVVSRTDNQNEWWIGRLNGAQGQFPGNYV 463 >SPBC11C11.02 |imp2||contractile ring protein Imp2|Schizosaccharomyces pombe|chr 2|||Manual Length = 670 Score = 46.8 bits (106), Expect = 2e-06 Identities = 21/54 (38%), Positives = 29/54 (53%), Gaps = 4/54 (7%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIA----HRRGLFPAXYV 456 V ALYD+ E+ FR+GD I V + WW+G + H RG FP+ +V Sbjct: 612 VSALYDYDAAIPEEISFRKGDTIAVLKLYEDGWWEGFVVGEDDHNRGQFPSNFV 665 >SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 45.6 bits (103), Expect = 4e-06 Identities = 23/52 (44%), Positives = 32/52 (61%), Gaps = 1/52 (1%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWW-QGXIAHRRGLFPAXYVT 459 V+ALY +T Q EL + GD+I VT+R+ + W +G + G FPA YVT Sbjct: 589 VKALYAYTAQSDMELSIQEGDIIQVTNRNAGNGWSEGILNGVTGQFPANYVT 640 >SPAC7D4.02c |||src |Schizosaccharomyces pombe|chr 1|||Manual Length = 415 Score = 39.5 bits (88), Expect = 3e-04 Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 4/62 (6%) Frame = +1 Query: 283 DVVPIEMLVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAH----RRGLFPAX 450 D PI V+ALY + E++ D + V +R WW+G + R GLFP+ Sbjct: 349 DGFPIFAYVRALYAYKATLPSEIDLNVDDTLVVLNRQKDGWWKGLVVSPTVGRIGLFPSN 408 Query: 451 YV 456 Y+ Sbjct: 409 YI 410 >SPBC19C2.10 |||BAR adaptor protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 39.5 bits (88), Expect = 3e-04 Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 7/62 (11%) Frame = +1 Query: 304 LVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWW-------QGXIAHRRGLFPAXYVTV 462 +V+ Y F P+ EL+ ++GD++ V D+ WW G G+FP+ Y Sbjct: 425 VVRMKYSFEPETENELKLKKGDLLLVLKEIDEGWWVGEKLGEDGVFTGNTGMFPSNYCVP 484 Query: 463 XH 468 H Sbjct: 485 AH 486 >SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 38.3 bits (85), Expect = 6e-04 Identities = 19/59 (32%), Positives = 33/59 (55%), Gaps = 3/59 (5%) Frame = +1 Query: 286 VVPIEMLVQALYDFTPQEAXELEFRRGDVITVTDR-SDQHWWQ--GXIAHRRGLFPAXY 453 + P +++ALYD+T ++A E+ F +GD V R +D+ W++ A RG P + Sbjct: 22 IEPPRKVIRALYDYTARKATEVSFAKGDFFHVIGRENDKAWYEVCNPAAGTRGFVPVSH 80 Score = 28.3 bits (60), Expect = 0.69 Identities = 13/38 (34%), Positives = 21/38 (55%) Frame = +1 Query: 292 PIEMLVQALYDFTPQEAXELEFRRGDVITVTDRSDQHW 405 P+ +VQ +DF + ELE + G+ I + RS+ W Sbjct: 125 PLFGIVQ--FDFAAERPDELEAKAGEAIIIIARSNHEW 160 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 37.9 bits (84), Expect = 9e-04 Identities = 16/52 (30%), Positives = 24/52 (46%) Frame = +1 Query: 304 LVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRRGLFPAXYVT 459 L ALYDF + E+ ++ ++I + + WW G PA YVT Sbjct: 1110 LYLALYDFAGRSPNEMTIKKDEIIEIVQKEPSGWWLALKNGAEGWVPATYVT 1161 >SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 420 Score = 37.9 bits (84), Expect = 9e-04 Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 7/60 (11%) Frame = +1 Query: 301 MLVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRR-------GLFPAXYVT 459 + V+A+Y FT + EL+ GDVI V+++ W+ G + + G+FP Y T Sbjct: 282 VFVKAIYSFTGRNEKELDLHTGDVIQVSEQLGPDWYMGEKVNSKAEKLGNSGMFPVNYCT 341 >SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces pombe|chr 1|||Manual Length = 886 Score = 36.7 bits (81), Expect = 0.002 Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 2/53 (3%) Frame = +1 Query: 304 LVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQG--XIAHRRGLFPAXYV 456 +V+ALY + + +L+F GD+I D WW G + +G+FP+ +V Sbjct: 10 MVRALYAWPGEREGDLKFTEGDLIECLSIGDGKWWIGRHINTNTQGIFPSNFV 62 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 31.9 bits (69), Expect = 0.056 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRRGLFPAXYV 456 ++ALYD+T Q E+ F+ + +D W + GL P YV Sbjct: 85 MKALYDYTQQSVDEISFQADQTLDCYGDTDSDWILVGFNNNFGLAPRNYV 134 >SPCC24B10.13 |skb5||Shk1 kinase binding protein 5|Schizosaccharomyces pombe|chr 3|||Manual Length = 140 Score = 29.5 bits (63), Expect = 0.30 Identities = 17/50 (34%), Positives = 21/50 (42%), Gaps = 2/50 (4%) Frame = +1 Query: 313 ALYDFTPQEAXELEFRRGDVITVTDRSDQHWW--QGXIAHRRGLFPAXYV 456 ALYDF P EL F G + + S W + R GL P +V Sbjct: 87 ALYDFEPLHDNELGFTTGQRLCILSESSDGWLIAYDDASGRSGLVPETFV 136 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.5 bits (63), Expect = 0.30 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQG 414 V ALY + + +LEF G VI V D W+ G Sbjct: 8 VVALYPYRSSFSDDLEFDPGQVIDVVSNLDGDWYTG 43 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 29.1 bits (62), Expect = 0.40 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +1 Query: 349 LEFRRGDVITVTDRSDQHWWQGXIAHRRGLFP 444 L+F GD I V + + W G + +RG FP Sbjct: 20 LKFSAGDTIIVIEVLEDGWCDGICSEKRGWFP 51 >SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 28.3 bits (60), Expect = 0.69 Identities = 13/52 (25%), Positives = 25/52 (48%), Gaps = 2/52 (3%) Frame = +1 Query: 307 VQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRR--GLFPAXYV 456 V A+ F +++ +GD++ + D S+ +WW + G PA Y+ Sbjct: 123 VHAIRGFEATVEGQVDATKGDMMILLDDSNSYWWLVKMCKNLAIGYLPAEYI 174 >SPBC16H5.11c |skb1|rmt5|type II protein arginine N-methyltransferase Skb1|Schizosaccharomyces pombe|chr 2|||Manual Length = 645 Score = 27.9 bits (59), Expect = 0.92 Identities = 20/54 (37%), Positives = 27/54 (50%), Gaps = 2/54 (3%) Frame = +1 Query: 1 RHEHSWYYGRITRADAEKLLANKPEGGFLIRISE--SSPGDFSLSVKCPDGVQH 156 R+ W G++T + N P+ I +SE S GD LS +C DGVQH Sbjct: 391 RNRQDWA-GKVTLVFGDMRTWN-PDYKIDILVSELLGSMGDNELSPECLDGVQH 442 >SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 27.1 bits (57), Expect = 1.6 Identities = 19/60 (31%), Positives = 29/60 (48%) Frame = -1 Query: 314 ACTNISMGTTSRSLSA*RRLQTRYDDNXASSLSELNLTTQRKNLLEASLNTLKCWTPSGH 135 A TN + TT++ A + R D + ASS S+ T K+ ++S + T SGH Sbjct: 568 ASTNAAATTTTKKPKAAKSTAVRDDVSSASSDSDKGTTGLGKSESKSSRKERRSSTSSGH 627 >SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1328 Score = 26.2 bits (55), Expect = 2.8 Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = -1 Query: 416 SPCHQCWSERSVTV-MTSPRLNSNSXASCGVKSYRACTNISMGTTSR 279 SP H E S+ + +T+P+ N+ A K+ + N+S GT SR Sbjct: 1185 SPYHGMQVEVSMVLNVTNPQFVVNTRAGKNRKTNVSANNVSQGTDSR 1231 >SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces pombe|chr 2|||Manual Length = 2493 Score = 25.8 bits (54), Expect = 3.7 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +1 Query: 145 GVQHFKVLRDASSKFFLWVVKFN 213 G++ F V RDA +FFLW + N Sbjct: 832 GIKEF-VNRDARKEFFLWALTIN 853 >SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces pombe|chr 3|||Manual Length = 1364 Score = 25.8 bits (54), Expect = 3.7 Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 3/67 (4%) Frame = -1 Query: 305 NISMGTTSRSLSA*RRLQTRYDDNXASSLSELNLTTQRKNL---LEASLNTLKCWTPSGH 135 N +G +S ++A +RLQ L +L+ T RK+L + + + W P H Sbjct: 76 NTVIGISSNVMAASKRLQME-------DLQQLSRETSRKSLNRRVSFASHARVRWYPKDH 128 Query: 134 LTDKEKS 114 +D EKS Sbjct: 129 QSDSEKS 135 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.0 bits (52), Expect = 6.5 Identities = 10/17 (58%), Positives = 13/17 (76%) Frame = +1 Query: 193 LWVVKFNSLNELAXLSS 243 +W +K+NSLN LA L S Sbjct: 690 VWNIKYNSLNALARLLS 706 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 25.0 bits (52), Expect = 6.5 Identities = 16/43 (37%), Positives = 21/43 (48%), Gaps = 1/43 (2%) Frame = -2 Query: 499 RTMMRAPXMTSXXPSRTKPGTT-RGGELXRPATSAGRSDLSRL 374 R+ +R S P K TT R G+L P + + RS SRL Sbjct: 622 RSHVRTGSNNSEAPLAAKTSTTKRNGDLLEPQSPSLRSHKSRL 664 >SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 24.6 bits (51), Expect = 8.5 Identities = 17/63 (26%), Positives = 31/63 (49%) Frame = -1 Query: 326 KSYRACTNISMGTTSRSLSA*RRLQTRYDDNXASSLSELNLTTQRKNLLEASLNTLKCWT 147 +S + +S+G+ S S+ R+LQT D S E N Q+K+ E ++++ + Sbjct: 69 QSRTTVSRVSLGSRQHSSSSIRKLQTNVSD--VRSYDERN---QKKSAFENFVSSMSSFL 123 Query: 146 PSG 138 G Sbjct: 124 TGG 126 >SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 24.6 bits (51), Expect = 8.5 Identities = 9/23 (39%), Positives = 15/23 (65%) Frame = +3 Query: 75 RWLLDKDQRIKPWRLLLISQMSG 143 R +LD+ + +PW L L+ Q+ G Sbjct: 125 RRVLDRTSKEEPWALRLLRQLEG 147 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,779,612 Number of Sequences: 5004 Number of extensions: 31503 Number of successful extensions: 126 Number of sequences better than 10.0: 28 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 200198394 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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